Magnetic resonance microimaging of cancer cell spheroid constructs

2017 ◽  
Vol 5 (1) ◽  
pp. 41-54 ◽  
Author(s):  
Konstantin I. Momot ◽  
Onur Bas ◽  
Nina Pauline Holzapfel ◽  
Daniela Loessner
Keyword(s):  
Magnetic Resonance ◽  
Cancer Cell ◽  
Cell Spheroid ◽  
Magnetic Resonance Microimaging

scholarly journals SPIONs/3D SiSBA-16 based Multifunctional Nanoformulation for target specific cisplatin release in colon and cervical cancer cell lines

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
B. Rabindran Jermy ◽  
Munther Alomari ◽  
Vijaya Ravinayagam ◽  
Sarah Ameen Almofty ◽  
Sultan Akhtar ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Magnetic Resonance ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Dapi Staining ◽  
Tem Analysis ◽  
Human Cancer Cell Lines

Abstract Multifunctional nanomaterials can be used for dual applications: drug delivery as well as in bioimaging. In current study, we investigated potential use of silica based supports; 3D cage type SiSBA-16 (S-16), monodispersed hydrophilic spherical silica (HYPS) and mesocellular foam (MSU-F) for cisplatin (Cp) delivery. To obtain magnetic resonance characteristics, 10 wt% iron oxide was loaded through enforced adsorption technique. For pH stimuli responsive release of Cp, 10 wt%SPIONs/S-16 was functionalized with 3-(Aminopropyl)triethoxysilane (A) and poly acrylic acid (PAA) termed as 10 wt%SPIONs/S-16-A-Cp and 10 wt%SPIONs/S-16-APAA-Cp. By TEM analysis, the average diameter of the SPIONs was found to range between 10–60 nm. VSM analysis showed saturation magnetization over S-16, HYPS and MSU-F were in the following order: 10 wt%SPIONs/HYPS (4.08 emug−1) > 10 wt%SPIONs /S-16 (2.39 emug−1) > 10 wt%SPIONs/MSU-F (0.23 emug−1). Cp release study using dialysis membrane in PBS solution over 10 wt%SPIONs/S-16 nanoformulations showed highest cumulative release (65%) than 10 wt%SPIONs/MSU-F-A-Cp (63%), 10 wt%SPIONs/HYPS-A-Cp (58%), and Cp-F127/S-16 (53%), respectively. 10 wt%SPIONs/S-16-A-Cp and 10 wt%SPIONs/S-16-APAA-Cp were evaluated for in vitro target anticancer efficiency in human cancer cell lines (colon cancer (HCT 116), cervical cancer (HeLa)) and normal cells (Human embryonic kidney cells (HEK293) using MTT and DAPI staining. 10 wt%SPIONs/S-16-A-Cp treated Hela and HCT116 cancerous cell lines showed significant control of cell growth, apoptotic activity and less cytotoxic effect as compared to Cp and 10 wt%SPIONs/S-16. Target specific Cp release in the cells shows that 10 wt%SPIONs/S-16-A-Cp can be easily upgraded for magnetic resonance imaging capability.

Application of Magnetic Resonance Microimaging to the Study of Dental Caries

1999 ◽  
Vol 34 (1) ◽  
pp. 53-58 ◽  
Author(s):  
C.H. Lloyd ◽  
S.N. Scrimgeour ◽  
J.A. Chudek ◽  
G. Hunter ◽  
R.L. MacKay
Keyword(s):  
Magnetic Resonance ◽  
Dental Caries ◽  
Magnetic Resonance Microimaging

Nuclear magnetic resonance microimaging of mouse spinal cord in vivo

2004 ◽  
Vol 15 (3) ◽  
pp. 474-482 ◽  
Author(s):  
Jean-Marie Bonny ◽  
Manuel Gaviria ◽  
Jean-Pierre Donnat ◽  
Beatrix Jean ◽  
Alain Privat ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Mouse Spinal Cord ◽  
Magnetic Resonance Microimaging ◽  
Nuclear Magnetic Resonance Microimaging ◽  
Nuclear Magnetic
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