scholarly journals THE EFFECT OF MORINGA LEAF EXTRACT (MORINGA OLEIFERA L) AGAINST MOTILITY OF SPERMATOZOA MICE EXPOSED TO MONOSODIUM GLUTAMATE

2022 ◽  
Vol 29 (1) ◽  
Author(s):  
Ilham Saptia Nugraha ◽  
Dimas Sindhu Wibisono ◽  
Indah Saraswati ◽  
Achmad Zulfa Juniarto
Keyword(s):  
Free Radicals ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Active Movement ◽  
Male Mice ◽  
Control Group Design ◽  
Anova Test ◽  
Post Test ◽  
The One ◽  
One Way Anova

Objective: Excessive use of MSG in everyday life can cause infertility to men. Exposure to MSG can cause cells to experience oxidative stress which subsequently triggers the generation of free radicals. Free radicals can be resisted with antioxidants. Material & Methods: This research was a laboratory experimental test with a post-test only control group design using male mice as the experimental object. The statistical test used the One Way ANOVA test. Results: In this research, 5 male mice were assigned into 4 groups and 1 control group. Using the One Way ANOVA test, it was found that there were significant differences in ≥ 2 treatment groups in the motility group of spermatozoa with active movement (p < 0.001) and the motility group of spermatozoa with weak movement (p = 0.036). The two groups showed significant differences. The Post hoc LSD test in the motility group with active movement showed significant differences between the control group with MSG treatment (p < 0.001) and the treatment group given Moringa leaves extract of 600 mg/kgW (p < 0.001) and 1200 mg/kgW (p < 0.001). Conclusion: Moringa leaves extract can effect the improvement of the motility of spermatozoa exposed to monosodium glutamate at a given dose of 300 mg/kgW and 600 mg/kgW.

THE EFFECT OF GINGER RHIZOMES (Curcuma xanthorriza Roxb.) TOWARDS THE NUMBER OF OVARIAN FOLLICLES OF FEMALE MICE (Mus musculus) EXPOSED TO MONOSODIUM GLUTAMAT (MSG)

2017 ◽  
Vol 2 (1) ◽  
pp. 12
Author(s):  
Meilina Ratna Dianti
Keyword(s):  
Monosodium Glutamate ◽  
Antioxidant Properties ◽  
Ovarian Follicles ◽  
Control Group ◽  
Endocrine Gland ◽  
Control Group Design ◽  
Female Mice ◽  
Post Test ◽  
One Way Anova ◽  
Pituitary Adrenal Axis

<p><em>Monosodium Glutamat activates an axis ablation in arcuate nucleus hypothalamus which happens to possibly initiate malfunctions in Hipothalamic Pituitary Adrenal axis. Yet, it is known that hypothalamus produces Gonadotropin Releasing Hormone which is able to stimulate the secretion of Gonadotropin Follicle Stimulating Hormon and Luteinizing Hormone from the anterior pituitary gland. These two hormones, however, are essentials for the development of gonad and during the cycles of oogenesis. Hence, any malfunctions resided in the human’s hypothalamus can possibly put endocrine gland into troubles, such as reproduction hormones which affect the workings of gonad. Therefore, the extract of Temulawak is promoted to be the attempt to resolve the issue for its antioxidant properties. Temulawak extract contains antioxidant, properties for as much as 87.01 ppm, according to Rosidi research’s result, analyzing the Temulawak’s potentials. The amount of antioxidant found Temulawak extract is categorized as active and applicable as natural antioxidant source. However, this research adopts true experimental post-test only control group design. It includes 5 (five) groups of female mice as which are K1 (CMC), K2 (CMC+MSG), P1 (MSG+0.4 mg Temulawak extract), P2 (MSG+0.8 MG Temulawak extract), P3 (MSG+1.2 mg Temulawak extract). In addition, the mice’s ovaries are removed to be subsequently tested using Hematoxillin-Eosin and the ovaries’ follicles quantity is observed and counted. The data is analyzed using One Way ANOVA. The research finally comes with a result showing that there are not any significant differences discovered from the ovaries’ follicles quantity from the five tested groups (p&gt;0.05). Therefore, it is implied that Temulawak extract does not provide any influential results to the house mice’s ovaries’ follicles quantity which has been exposed to MSG</em>.</p><p><em> </em></p><strong><em>Keywords</em></strong><em>: Monosodium Glutamate (MSG), Temulawak (Curcuma xanthorriza Roxb.), Ovarian follicles quantity </em>

THEEFFECTS OF CURCUMA RHIZOME EXTRACT (Curcuma xanthorrhiza Roxb.)TREATMENT USING VARIOUS DOSES TOWARDS ENDOMETRIAL THICKNESSIN MICE (Mus musculus) TREATED WITH MONOSODIUM GLUTAMATE (MSG)

2017 ◽  
Vol 2 (1) ◽  
pp. 39
Author(s):  
Fidia Rizkiah Inayatilah
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Endometrial Thickness ◽  
Monosodium Glutamate ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Curcuma Xanthorrhiza ◽  
One Way Anova

<p><em>Monosodium Glutamate </em>(MSG) is a food seasoning that is widely used. Consumption of MSG in excessive doses can cause an excess of free radicals in the body that trigger oxidative stress and finally affect the reproductive system hormones. These free radicals can be suppressed by administration of antioxidants. Rhizomes of Curcuma xanthorrhiza extracts containantioxidants and they are widely grown. Theyare used as traditional medicine in Indonesia. This experiment was conducted to investigatewhether rhizomes of Curcuma xanthorrhiza extracts areable to inhibit decreasing endometrial thickness in female mice after receiving orally MSG-treatment.This research adoptedtrue experimental posttest only control group design<em>. </em>It includes 25 mice that were divided into 5 groups: control K1 (CMC) and K2 (MSG), treatment group (MSG and rhizomes of Curcuma xanthorrhiza extracts with dose of 0,4 mg/gBW, 0,8 mg/gBW, 1,2 mg/gBW).In addition, the mice’s endometrium were removed to be subsequently stained using Hematoxillin-Eosin. Thickening of uterus was observed and measured with 40x magnitude using Microphoto Microscope Olympus. Data was analized using One Way ANOVA. The research finally comes with a result showing that there were not any significant differences discovered from the endometrium thickening from the five tested groups (p&gt;0,05). Therefore, it is implied that rhizomes of Curcuma xanthorrhiza extracts has no effect on endometrial thickness of uterus by oral MSG-treatment.</p><p> </p><p>Key words: MSG, curcuma, endometrium</p>

scholarly journals THE EFFECT OF GINGER RHIZOMES (Curcuma xanthorriza Roxb.) TOWARDS THE NUMBER OF OVARIAN FOLLICLES OF FEMALE MICE (Mus musculus) EXPOSED TO MONOSODIUM GLUTAMAT (MSG)

2017 ◽  
Vol 2 (1) ◽  
pp. 12
Author(s):  
Meilina Ratna Dianti
Keyword(s):  
Monosodium Glutamate ◽  
Antioxidant Properties ◽  
Ovarian Follicles ◽  
Control Group ◽  
Endocrine Gland ◽  
Control Group Design ◽  
Female Mice ◽  
Post Test ◽  
One Way Anova ◽  
Pituitary Adrenal Axis

<p><em>Monosodium Glutamat activates an axis ablation in arcuate nucleus hypothalamus which happens to possibly initiate malfunctions in Hipothalamic Pituitary Adrenal axis. Yet, it is known that hypothalamus produces Gonadotropin Releasing Hormone which is able to stimulate the secretion of Gonadotropin Follicle Stimulating Hormon and Luteinizing Hormone from the anterior pituitary gland. These two hormones, however, are essentials for the development of gonad and during the cycles of oogenesis. Hence, any malfunctions resided in the human’s hypothalamus can possibly put endocrine gland into troubles, such as reproduction hormones which affect the workings of gonad. Therefore, the extract of Temulawak is promoted to be the attempt to resolve the issue for its antioxidant properties. Temulawak extract contains antioxidant, properties for as much as 87.01 ppm, according to Rosidi research’s result, analyzing the Temulawak’s potentials. The amount of antioxidant found Temulawak extract is categorized as active and applicable as natural antioxidant source. However, this research adopts true experimental post-test only control group design. It includes 5 (five) groups of female mice as which are K1 (CMC), K2 (CMC+MSG), P1 (MSG+0.4 mg Temulawak extract), P2 (MSG+0.8 MG Temulawak extract), P3 (MSG+1.2 mg Temulawak extract). In addition, the mice’s ovaries are removed to be subsequently tested using Hematoxillin-Eosin and the ovaries’ follicles quantity is observed and counted. The data is analyzed using One Way ANOVA. The research finally comes with a result showing that there are not any significant differences discovered from the ovaries’ follicles quantity from the five tested groups (p&gt;0.05). Therefore, it is implied that Temulawak extract does not provide any influential results to the house mice’s ovaries’ follicles quantity which has been exposed to MSG</em>.</p><p><em> </em></p><p><strong><em>Keywords</em></strong><em>: Monosodium Glutamate (MSG), Temulawak (Curcuma xanthorriza Roxb.), Ovarian follicles quantity </em></p>

scholarly journals Pengaruh Perendaman Minuman Berkarbonasi terhadap Gaya Elastik pada Elastomerik Ligatur dengan Variasi Panjang Penarikan

e-GIGI ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 282
Author(s):  
Hafidz I. Pradipta ◽  
Budi Wibowo ◽  
Diah A, Purbaningrum ◽  
Yoghi B. Prabowo
Keyword(s):  
Testing Machine ◽  
Elastic Force ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Post Test ◽  
Carbonated Drinks ◽  
The One ◽  
Post Hoc ◽  
One Way Anova

Abstract: Elastomeric ligature is used to fasten orthodontic wires with brackets and can be stretched 1-3 mm as a simulation of bracket in the oral cavity. Its elastic force could decrease caused by salivary pH and water absorption of the elastomeric ligature. Consuming carbonated drinks causes the oral cavity to become acidic which increases the loss of elastic force in the elastomeric ligature with length of stretch variations. This study was aimed to determine the effect of immersion in carbonated drinks on the elastic force of the elastomeric ligature with length of stretch variations. This was an experimental laboratory study with a post-test only control group design. There were a total of 24 samples of American Orthodontic brand elastomeric ligature with royal blue color divided into six groups, consisting of three control groups immersed in artificial saliva, and three treatment groups immersed in carbonated drinks. Each control group and treatment group were given three variations of stretch length, as follows: 1, 2, and 3 mm. The calculation of the elastic force on the elastomeric ligature was performed by using the Universal Testing Machine. Data were analyzed by using the One Way Anova test and LSD post hoc test (p <0.05). The One Way Anova test showed a significant difference in the 1, 2, and 3 mm length of stretch variations for treatment groups. In conclusion, carbonated drinks affect the elastic force of elastomeric ligature with length of stretch variations.Keywords: elastomeric ligature; carbonated drinks; length of stretch variations Abstrak: Elastomerik ligatur adalah alat yang digunakan untuk mengikatkan kawat ortodonti dengan braket, dapat diregangkan 1-3 mm sebagai simulasi braket dalam rongga mulut. Elastomerik ligatur mengalami penurunan gaya elastik salah satunya disebabkan oleh pH saliva, dan penyerapan air dari elastomerik ligatur. Mengonsumsi minuman berkarbonasi menyebabkan pH saliva menjadi asam sehingga meningkatkan kehilangan gaya elastik pada elastomerik ligatur dengan variasi panjang penarikan. Tujuan penelitian ini untuk mengetahui pengaruh perendaman minuman berkarbonasi terhadap gaya elastik pada elastomerik ligatur dengan variasi panjang penarikan. Desain penelitian ialah eksperimental laboratorium dengan post-test only control group design. Sebanyak 24 sampel elastomerik ligatur merek American Orthodontic dengan warna royal blue, dibagi menjadi enam kelompok, terdiri dari tiga kelompok kontrol perendaman pada saliva buatan, dan tiga kelompok perlakuan perendaman pada minuman berkarbonasi. Setiap kelompok kontrol dan perlakuan diberi tiga variasi panjang penarikan, yaitu penarikan 1, 2, dan 3 mm. Perhitungan gaya elastik pada elastomerik ligatur menggunakan alat Universal Testing Machine. Analisis data menggunakan uji One Way Anova dan uji post hoc LSD pada p<0,05. Hasil uji One Way Anova menunjukkan perbedaan bermakna pada penarikan 1, 2, 3 mm dalam perendaman minuman berkarbonasi. Simpulan penelitian ini ialah terdapat pengaruh perendaman minuman berkarbonasi terhadap gaya elastik pada elastomerik ligatur dengan variasi panjang penarikan.Kata kunci: elastomerik ligatur; minuman berkarbonasi; variasi panjang penarikan

scholarly journals Pengaruh perendaman plat resin akrilik dalam larutan kopi dengan berbagai kekentalan terhadap perubahan volume larutan kopi

e-GIGI ◽  
2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Rachel S. Togatorop ◽  
Jimmy F. Rumampuk ◽  
Vonny N.S. Wowor
Keyword(s):  
Base Material ◽  
Acrylic Resin ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Denture Base ◽  
Group Design ◽  
Post Test ◽  
The One ◽  
One Way Anova

Abstract: Acrylic resin is a denture base material that is still used in the field of dentistry. Porosity, one of the properties of a denture base material, can lead to absorption of fluid in the mouth, one of them is coffee. Porosity that occurs in a denture base material can cause discoloration. Coffee is favored by most people. Coffee contains tannins, a polyphenol compound, that can be absorbed through the porous of the denture base which can cause discoloration of the denture base. This study was aimed to determine the effect of acrylic resin plate immersed in coffee solution with various viscosity to the changes in volume of coffee solution. This was a pre laboratory experimental study with a pre and post test only control group design. Samples were 18 acrylic resin plates with a size of 65 x 10 x 2.5 mm. Each treatment group consisted of 6 samples. The samples were divided into 3 treatment groups that were soaked in coffee 2 g, 4 g, and 6 g for 7 days. The results showed that after 7 days of immersing resin acrylic plates in coffee solution with various viscosity, the changes of the volumes of coffee solution had a mean of 0.064 for 2 g; 0.147 for 4 g; and 0.244 for 6 g. The one-way ANOVA test resulted in a p value of 0.00. Conclusion: There was a significant effect of resin acrylic immersed in a coffee solution with various viscosity to the changes in the volume of coffee solution.Keywords: acrylic resin, changes in volume of the solution of coffee, coffee Abstrak: Resin akrilik merupakan bahan basis gigi tiruan yang sampai saat ini masih digunakan dalam bidang Kedokteran Gigi. Porositas merupakan salah satu sifat basis gigi tiruan. Adanya porositas pada basis gigi tiruan dapat mengakibatkan terserapnya cairan yang masuk dalam mulut salah satunya yaitu kopi. Porositas yang terjadi pada basis gigi tiruan dapat menyebabkan perubahan warna pada basis gigi tiruan. Kopi merupakan salah satu minuman yang banyak digemari masyarakat. Kopi mengandung tanin suatu senyawa polifenol yang dapat terserap masuk melalui porus pada basis gigi tiruan dan menyebabkan perubahan warna pada basis gigi tiruan. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman plat resin akrilik dalam larutan kopi dengan berbagai kekentalan terhadap perubahan volume larutan kopi. Jenis penelitian ialah pra eksperimental laboratorium dengan pre and post test only control group design. Sampel terdiri dari 18 plat resin akrilik dengan ukuran 65 x 10 x 2,5 mm. Sampel dibagi menjadi 3 kelompok perlakuan yang di rendam dalam kopi 2gr, 4gr, dan 6gr selama 7 hari. Masing-masing kelompok perlakuan terdiri dari 6 sampel. Hasil penelitian mendapatkan setelah 7 hari perendaman plat akrilik dalam larutan kopi dengan berbagai kekentalan terhadap perubahan volume larutan kopi memiliki rerata kopi 2 gr sebesar 0,064, kopi 4 gr sebesar 0,147. dan kopi 6 gr sebesar 0,244. Uji One-way Anova menunjukkan adanya pengaruh perendaman resin akrilik dalam larutan kopi dengan berbagai kekentalan terhadap perubahan volume larutan kopi dengan p=0,00. Simpulan: Terdapat pengaruh bermakna dari perendaman resin akrilik dalam larutan kopi dengan berbagai kekentalan terhadap perubahan volume larutan kopiKata kunci: resin akrilik, perubahan volume larutan kopi, kopi

scholarly journals THEEFFECTS OF CURCUMA RHIZOME EXTRACT (Curcuma xanthorrhiza Roxb.)TREATMENT USING VARIOUS DOSES TOWARDS ENDOMETRIAL THICKNESSIN MICE (Mus musculus) TREATED WITH MONOSODIUM GLUTAMATE (MSG)

2017 ◽  
Vol 2 (1) ◽  
pp. 38
Author(s):  
Fidia Rizkiah Inayatilah
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Endometrial Thickness ◽  
Monosodium Glutamate ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Curcuma Xanthorrhiza ◽  
One Way Anova

<p><em>Monosodium Glutamate </em>(MSG) is a food seasoning that is widely used. Consumption of MSG in excessive doses can cause an excess of free radicals in the body that trigger oxidative stress and finally affect the reproductive system hormones. These free radicals can be suppressed by administration of antioxidants. Rhizomes of Curcuma xanthorrhiza extracts containantioxidants and they are widely grown. Theyare used as traditional medicine in Indonesia. This experiment was conducted to investigatewhether rhizomes of Curcuma xanthorrhiza extracts areable to inhibit decreasing endometrial thickness in female mice after receiving orally MSG-treatment.This research adoptedtrue experimental posttest only control group design<em>. </em>It includes 25 mice that were divided into 5 groups: control K1 (CMC) and K2 (MSG), treatment group (MSG and rhizomes of Curcuma xanthorrhiza extracts with dose of 0,4 mg/gBW, 0,8 mg/gBW, 1,2 mg/gBW).In addition, the mice’s endometrium were removed to be subsequently stained using Hematoxillin-Eosin. Thickening of uterus was observed and measured with 40x magnitude using Microphoto Microscope Olympus. Data was analized using One Way ANOVA. The research finally comes with a result showing that there were not any significant differences discovered from the endometrium thickening from the five tested groups (p&gt;0,05). Therefore, it is implied that rhizomes of Curcuma xanthorrhiza extracts has no effect on endometrial thickness of uterus by oral MSG-treatment.</p><p> </p><p><strong>Keywords</strong>: MSG, curcuma, endometrium</p>

scholarly journals Efektifitas Debridemen Mekanik Pada Luka Bakar Derajat III Terhadap Kecepatan Penyembuhan Luka

JKEP ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 93-103
Author(s):  
Nurma Afiani ◽  
Sanarto Santoso ◽  
Tina Handayani N ◽  
M. Fendi Nur Yahya
Keyword(s):  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Degree Burn ◽  
Significant Difference ◽  
Post Test ◽  
Long Time ◽  
The One ◽  
Post Hoc ◽  
One Way Anova

Third degree burn is a special wound cause consist of eschar for a long time. Kept eschar in third degree burn will delay healing procces. That is why eschar should be disapper by debridement process. Mechanical debridement used in this research. The aim of this research is to know the differences between mechanical debridement 24 hour and 48 hour in healing acceleration. This research is a true experimental by using  post test only control group design. The samples was selected by simple random sampling and was divided into three groups, each group consist of six rats, they are 24 hour group, 48 hour group and control group. The data was analyzed by one-way ANOVA then followed by Post Hoc LSD. The one way ANOVA result  p = 0,018 (p < 0,05) so there was a significant difference between control group and experimental groups. But the Post Hoc LSD results p = 0,123 ( p > 0,05)  so there was no significant difference between 24 hour group and 48 hour group in healing acceleration. For further researchers, it is suggested to use microscopic technique to know the effect of mechanical debridement in healing acceleration.

Pengaruh Pemberian Ekstrak Rumput Laut Merah (Kappaphycus alvarezii) terhadap Kadar LDL Pada Tikus Putih (Rattus Norvegicus) Jantan Galur Wistar Yang Diberi Diet Tinggi Lemak

2018 ◽  
Vol 15 (2) ◽  
pp. 112
Author(s):  
MENTARI AMENDA SAPUTRI ◽  
HERIN SETIANINGSIH
Keyword(s):  
Rattus Norvegicus ◽  
Kappaphycus Alvarezii ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova ◽  
Test Control

<p class="Default">Penyakit kardiovaskular merupakan penyebab kematian nomor satu di dunia. Gaya hidup masyarakat terutama dalam mengkonsumsi diet yang tidak sehat dapat meningkatkan kadar LDL yang dapat menyebabkan  penyakit kardiovaskular. Rumput laut merah (<em>Kappaphycus alvarezii</em>)<em> </em>yang banyak dibudidayakan di Indonesia mengandung flavonoid dan triterpenoid yang diduga dapat menurunkan kadar LDL. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) <em> </em>terhadap kadar LDL pada tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang diberi diet tinggi lemak. Penelitian ini adalah penelitian eksperimental murni laboratorik dengan rancangan penelitian <em>Post Test Control Group Design. </em>Sampel yang digunakan adalah 24 ekor tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang dibagi ke dalam tiga kelompok: kelompok yang diberi diet standar selama 28 hari (K1), kelompok yang diberi diet tinggi lemak selama 28 hari (K2), dan kelompok yang diberi diet tinggi lemak selama 28 hari dan pada hari ke-15 sampai hari ke-28 diberi ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) dengan dosis 140mg/200grBB/hari (K3). Hasil analisis statistik <em>One Way Anova </em>menunjukkan bahwa terdapat perbedaan kadar LDL yang signifikan antara ketiga kelompok pada penelitian ini (p&lt;0,001). Kadar LDL pada K2 (=16,00±3,29) meningkat secara bermakna dibandingkan dengan K1 (=10,62±1,77). Sedangkan kadar LDL pada K3 (=6,88±2,42) menurun secara bermakna dibandingkan dengan K2. Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) berpengaruh terhadap kadar LDL darah pada tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang diberi diet tinggi lemak.</p><p><strong>Kata kunci</strong> : diet tinggi lemak, LDL, <em>Kappaphycus alvarezii</em></p>

Application of Hydroxyapatite scaffold from Portunus pelagicus on OPG and RANKL expression after tooth extraction of Cavia cobaya

2021 ◽  
pp. 4647-4651
Author(s):  
Michael Josef Kridanto Kamadjaja ◽  
Sherman Salim ◽  
Birgitta Dwitya Swastyayana Subiakto
Keyword(s):  
Tooth Extraction ◽  
Alveolar Bone ◽  
Control Group ◽  
Rankl Expression ◽  
Control Group Design ◽  
Portunus Pelagicus ◽  
Group Design ◽  
Post Test ◽  
Hydroxyapatite Scaffold ◽  
One Way Anova

Objective: This study was to determine OPG and RANKL expression after hydroxyapatite (HA) scaffold from crab shells (Portunus pelagicus) application in tooth socket of Cavia cobaya. Methods: This study was a post-test only control group design. Twenty four Cavia cobaya was divided into 4 groups. The lower left incisor was extracted and given a combination of HA and gelatin scaffold. Experimental animals were sacrificed on the 7th and 14th day. The amount of OPG and RANKL expression was calculated under a light microscope at 1000x magnification. The statistical analysis was done by One Way ANOVA Test and Tukey HSD. Results: Compared to other groups, the lowest and the highest level of OPG and RANKL were in P14 group. Conclusion: HA scaffold from crab shells (Portunus pelagicus) can increase OPG expression and decrease RANKL expression in the process of regenerating alveolar bone after tooth extraction.

scholarly journals Status Kerentanan Aedes Aegypti Vektor Demam Berdarah Dengue di Kota Padang

2017 ◽  
Vol 6 (2) ◽  
pp. 440
Author(s):  
Kharisma Putra D ◽  
Hasmiwati Hasmiwati ◽  
Arni Amir
Keyword(s):  
Aedes Aegypti ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Salah satu upaya untuk mengurangi kasus DBD adalah dengan pengendalian vektor DBD dengan larvisida. Temephos merupakan salah satu insektisida yang telah digunakan lebih dari 30 tahun dan berfungsi mengendalikan larva vektor. Penggunaan temephos yang tidak sesuai aturan dapat menyebabkan penurunan kerentanan pada vektor DBD.  Tujuan penelitian ini  adalah untuk  menilai  status  kerentanan larva Aedes aegypti di tiga kecamatan di Kota Padang. Penelitian ini menggunakan post  test  only  with  control  group design. Telur diambil dan dipelihara di laboratorium hingga mencapai larva instar III/IV. Uji kerentanan untuk temephos dilakukan berdasarkan standar WHO. Hasil penelitian menunjukkan pada Kecamatan Kuranji, kematian larva pada konsentrasi 0,005 mg/L sebesar 10%, 0,01 mg/L sebesar 45%, 0,02 mg/L sebesar 86%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Koto Tangah, kematian larva pada konsentrasi 0,005 mg/L sebesar 24%, 0,01 mg/L sebesar 48%, 0,02 mg/L sebesar 99%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Padang Timur pada konsentrasi 0,005 mg/L didapatkan kematian larva sebesar 12%, pada 0,01 mg/L sebesar 43%, pada 0,02 mg/L sebesar 99%, dan pada 0,03 mg/L sebesar 100%. Hasil uji One way-Anova adalah bermakna dengan nilai p<0,05 pada ketiga kecamatan dan LC99 sedikit diatas 0,02 mg/L. Simpulan penelitian ini adalah status kerentanan Aedes aegypti terhadap temephos di tiga kecamatan berkisar antara rentan dan toleran, belum mencapai resisten sehingga temephos masih dapat digunakan dalam pengendalian vektor DBD.

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