scholarly journals nxiolytic Effect of the Hydro-alcoholic Extract of Anethum Graveolens Seed in Adult Female Wistar Rats: Modulation of GABA Receptors

2022 ◽  
Vol 8 (1) ◽  
pp. 1-6
Author(s):  
Rana Shahabi ◽  
◽  
Mohammad Rostampour ◽  
Behrooz Khakpour ◽  
Bahram Soltani ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Gaba Receptors ◽  
Industrial Development ◽  
Wistar Rats ◽  
Anxiolytic Effect ◽  
Control Group ◽  
Alcoholic Extract ◽  
Gamma Aminobutyric Acid ◽  
Anethum Graveolens ◽  
Female Wistar Rats

Background: Along with industrial development and the increasing social complexity of societies, anxiety is one of the most prevalent psychological disorders. Medicinal plants are considered as an enrichment source of ingredients with biological activity. Objectives: The aim of this study was to evaluate the anxiolytic effect of Anethum Graveolens seed (AGS) and the possible involvement of Gamma-Aminobutyric Acid (GABA)-ergic system in the AGS effect. Materials & Methods: In the present experimental study, 64 female Wistar rats were divided into eight groups and received various concentrations of hydroalcoholic extract of AGS. To measure the level of anxiety, an elevated plus maze was used in a way that the animal’s head turned to an open arm. Prior to the injections of AGS extract, the GABA receptor antagonist was used. The results were analyzed by one-way analysis of variance using IBM SPSS v. 16. Results: Dose-response experiments showed that the AGS extract significantly decreased the anxiety indices compared to the control group (P<0.05). To analyze locomotor activity, our data showed that AGS extract at 0.1, 1, and 10 mg/kg could significantly increase locomotor activity compared to the control group (P<0.001). Pentylenetetrazol (PTZ (+extract significantly decreased the anxiolytic effect of AGS extract (P<0.01). Conclusion: Considering the anti-anxiety effects of AGS extract and a reduction in this effect caused by PTZ, part of the anti-anxiety effect of extract might be assumed via its interaction with GABA-ergic receptors. Further experimental trials; however, are required for the establishment of the anti-anxiety impact of AGS.

Curative effects of Dhatryadi ghrita bioactive extracts on ethanol withdrawal syndrome in Wistar rats

2020 ◽  
Vol 16 ◽  
Author(s):  
Rashmi Saxena Pal ◽  
Amrita Mishra
Keyword(s):  
Locomotor Activity ◽  
Alcohol Withdrawal ◽  
Withdrawal Syndrome ◽  
Wistar Rats ◽  
Alcohol Withdrawal Syndrome ◽  
Signs And Symptoms ◽  
Normal Diet ◽  
Ethanol Withdrawal ◽  
Control Group ◽  
Alcoholic Extract

Background: Alcohol withdrawal syndrome leads to irritability, aggressiveness, body posture and motor abnormalities, sensory hyper reactivity and changes in various enzyme levels. Dhatryadi ghrita penetrates the blood- brain barrier to decrease the cravings for alcohol in this syndrome. Objective: To evaluate the effect of alcoholic extract of Dhatryadi ghrita on alcohol withdrawal syndrome in Wistar rats. Material & Methods: A liquid diet with 7.2%, v/v ethanol was administered to the Wistar rats for 21 days. Control group animals received saline and normal diet. After alcohol withdrawal, rats were examined at 6th and 24th hour for anxiety and hyper locomotor activity as major withdrawal signs. Anxiety due to ethanol withdrawal was tested with the help of elevated plus maze, light and dark models. The hyper locomotor activity was assessed using Actophotometer. The hepatic enzymes level was determined with the help of the Bio-chemical Analyzer. Ghrita extract (100, 200,300 mg/kg, oral) were administered to different groups and diazepam as standard (2 mg/kg, i.p) were administered to the treatment group animals 30 minutes before alcohol withdrawal estimation. Drug treatment was administered 30 minutes before the second observation at the 24th hour. Results: Findings from the present study revealed that Ghrita extract treatment at doses 100, 200 and 300 mg/kg, oral in ethanol-dependent rats had a significant protective effect on signs and symptoms of ethanol withdrawal in alcohol-dependent rats. Conclusion: Dhatryadi extract acts effectively for the treatment of alcohol abstinence syndrome. The extract treat¬ment has beneficial effects on ethanol withdrawal depressive-like behavior in rats.

scholarly journals Effects of alcoholic extract of Myristica fragrans (nutmeg) on pregnancy outcome in female Wistar rats

2021 ◽  
Vol 25 (8) ◽  
pp. 1483-1491
Author(s):  
C.L. Sakpa ◽  
D.O. Eguavoen
Keyword(s):  
Wistar Rats ◽  
Ovarian Follicles ◽  
Control Group ◽  
Alcoholic Extract ◽  
Alcohol Extract ◽  
Orogastric Tube ◽  
Group 4 ◽  
Myristica Fragrans ◽  
Female Wistar Rats ◽  
Group 2

Myristica fragrans has been reported to be a medicinal plant as it possesses hepatoprotective, antimicrobial, anti-oxidant, antiulcer, anticancer and immunostimulatory properties, as well as antifertility potentials. This study therefore aimed to investigate the effects of alcoholic extract of M. fragrans on dam size, litter size, pup weight, ovarian follicles, endometrial glands and histopathological indices on pregnancy outcomes in female Wistar rats using standard methods. Pregnancy was induced in forty-eight (48) female Wistar rats that were randomly selected and assigned into four (4) groups of twelve (12) rats each: Group 1 (pregestational phase of pregnancy), Group 2 (germinal phase of pregnancy), Group 3 (embryonic phase of pregnancy) and Group 4 (Fetal phase of pregnancy). The treated animals all received 1500 mg of alcoholic extract of M. fragrans. Administration of distilled water and the extract was by orogastric tube. Data were subjected to statistical analysis using the IBM SPSS statistics software (Statistical Package for Social Science) (Version 25) and relevant statistical values were obtained. The extract prevented the proliferation of ovarian follicles and endometrial glands. In addition, during the pregestational, germinal and embryonic phases, there were significant increases in weight of pups when the treated animals were compared to the control group. The dams also showed decreases in body weight during the embryonic phase. This study therefore demonstrated that alcohol extract of Myristica fragrans may serve as a contraceptive agent in view of its anti-fertility effect.

scholarly journals ANTIFUNGAL EFFECT OF NIGELLA SATIVA EXTRACT ON FEMALE WISTAR RATS VULVOVAGINAL CANDIDIASIS MODEL

2020 ◽  
pp. 66-68
Author(s):  
MUHAMMAD RUSDA
Keyword(s):  
Candida Albicans ◽  
Therapeutic Effect ◽  
Wistar Rats ◽  
Nigella Sativa ◽  
Vulvovaginal Candidiasis ◽  
Analytic Study ◽  
Control Group ◽  
Antifungal Effect ◽  
Female Wistar Rats

Objective: To assess the antifungal effect of Nigella sativa extract on female Wistar rats vulvovaginal candidiasis. Methods: This was an analytic study that assess the therapeutic effect of Nigella sativa in rats’ vulvovaginal candidiasis model. The subjects were 28 rats that had been inoculated with Candida albicans and divided into 4 groups: control group (G1), fluconazole group (G2), Nigella sativa group (G3) and combination of Nigella sativa with fluconazole group (G4). Candida albicans colony was measured to assess the therapeutic effect of the treatment. Results: There were no difference number of Candida albicans colony between all group before inoculation (p = 0,274) and after inoculation (p = 0,323). There were a significant decreased number of Candida albicans colony on the 72 h after the treatment between the three types of treatment with the control group (Nigella sativa group p = 0,002; Fluconazole group p = 0,001; Nigella sativa+fluconazole p = 0,001) Conclusion: Nigella sativa has a potential antifungal effect by reducing the number of Candida albicans colonies.

scholarly journals Comparison of the absorption efficiency of α- and β-cryptoxanthin in female Wistar rats

2007 ◽  
Vol 97 (2) ◽  
pp. 329-336 ◽  
Author(s):  
Dietmar E. Breithaupt ◽  
Elhadi M. Yahia ◽  
Francisco J. Valdés Velázquez
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Corn Oil ◽  
Absorption Efficiency ◽  
Array Detector ◽  
Control Group ◽  
Plasma Samples ◽  
Female Wistar Rats ◽  
Positive Correlations ◽  
Rat Livers

Xanthophylls, such as lutein and zeaxanthin, have received increasing interest in recent years because of positive correlations between their consumption and the prevention of eye diseases. Numerous human intervention studies have been conducted with lutein to estimate the bioavailability from different formulations. The present study was designed to obtain basic data on the absorbance efficiency of the monohydroxylated counterparts of lutein and zeaxanthin: α- and β-cryptoxanthin. A corn-oil-based diet comprising ß-cryptoxanthin from papaya purée and α-cryptoxanthin from green carrot leaves was fed to five female Wistar rats for 8 consecutive days at a rate of 17·3 nmol/d and 9·2 nmol/d, respectively. The identity of the xanthophylls in the supplement was ascertained by LC-(APCI)MS analyses, and xanthophylls present in liver and plasma samples were determined by HPLC/diode array detector (DAD). The β-cryptoxanthin concentrations of rat livers in the treatment group were statistically distinguishable (P < 0·01) from those present in the livers of the control group that were fed a basic diet. α-Cryptoxanthin, the second xanthophyll present in the supplement, was not found in rat livers in the treatment group. Plasma samples were free of xanthophylls. This is the first report proving that β-cryptoxanthin has a higher absorption efficiency than α-cryptoxanthin in rats, at least from a minimally processed oil-based xanthophyll supplement.

scholarly journals Fluoxetine effect on gestation and fetal development

2014 ◽  
Vol 60 (4) ◽  
pp. 157-159
Author(s):  
Bianca Eugenia Ösz ◽  
C. E. Vari ◽  
Maria Dogaru
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Fetal Development ◽  
Wistar Rats ◽  
Selective Serotonin Reuptake Inhibitors ◽  
Gravid Uterus ◽  
Control Group ◽  
Increased Risk ◽  
Female Wistar Rats ◽  
In Pregnancy

Abstract The prenatal exposure to selective serotonin reuptake inhibitors (SSRIs) is very controversial. There is no conclusive evidence for increased risk of malformations after SSRI use in pregnancy. The aim of the study was to determine how fluoxetine is affecting gestation and fetal development in rats. Twenty sexually mature female Wistar rats weighting between 250-260 g received 20 mg/kg body weight fluoxetine from the first day of gestation and during the entire gestation period.The drug was administered by oral route. Healthy, primipareus animals were selected along with 20 female Wistar rats, as control group. Mature males were caged with virgin females for an entire week. Rat’s behaviour during gestation, after birth and rats body weight was examined. The number of healthy pups was also noted. The females not giving birth after 21 days to any pup were anesthetized (halothane through gas scavenging apparatus untilled death) and the gravid uterus were dissected out and examined. Compared to the controlled group, in which weight gain was more significant, the animals from the experimental group had a slight increase in body weight. The weight gain normally induced by gestation, is less significant in fluoxetine treated rats due to the increase serotonin levels in the brain. The uteri examination of pregnant rats showed an increase in the number of dead and resorbed rat embryos. Preclinical studies suggest that the inclusion of fluoxetine in pregnancy category C is justified and the appropriateness of its administration in pregnancy is still an unresolved issue.

scholarly journals Mung bean sprout extract suppresses Monosodium Glutamate (MSG) effect on the reproductive hormones (FSH and Estrogen) in female Wistar rats

2019 ◽  
Vol 27 (1) ◽  
pp. 24 ◽  
Author(s):  
Widati Fatmaningrum ◽  
Woro Setia Ningtyas
Keyword(s):  
Rattus Norvegicus ◽  
Mung Bean ◽  
Wistar Rats ◽  
Monosodium Glutamate ◽  
Follicle Stimulating Hormone ◽  
Control Group ◽  
Bean Sprout ◽  
Mung Bean Sprouts ◽  
Female Wistar Rats ◽  
Bean Sprouts

Objectives: The purpose of this study was to analyze the effects of mung bean sprout extracts on Follicle stimulating hormone (FSH) and estrogen hormone in female Wistar rats (Rattus norvegicus) exposed to monosodium glutamate (MSG).Materials and Methods: This true experimental study was conducted by using post-test only control group design in the laboratory for animal experimentation of Faculty of Veterinary Medicine - Airlangga University in which Wistar rats (Rattus norvegicus) aged 2 months weighing 150-200 grams were used in this experiment. The samples comprised of 5 rats distributed in each group, totaling 7 groups. I Control Group (P1) was provided with Aquades for 37 days; II (P2) was provided with Aquades for 7 days + MSG 0.03 mg/g of weight on day 8-37; III (P3) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0,03mg/g of weight; IV (P4) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.03mg/g of weight; V (P5) was provided with Aquades for 7 days + MSG 0.7 mg/g of weight on day 8-37; VI (P6) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight; VII (P7) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight.Serum samples were taken for Follicle Stimulating Hormone (FSH) and Estrogen with ELISA method. Data analysis to test the differences between groups was done by using one way Anova statistical test.Results: MSG dosage 0,03mg/gBB or 0,7mg/g BB was significant difference in FSH (p = 0,011) and estrogen (p = 0,008).Conclusion: obtained from this research that giving green bean sprout extract influence to the level of FSH and estrogen hormone.

scholarly journals Methyl Gallate Attenuates Doxorubicin-Induced Cardiotoxicity in Rats by Suppressing Oxidative Stress

Scientifica ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Akheruz Zaman Ahmed ◽  
Shakta Mani Satyam ◽  
Prakashchandra Shetty ◽  
Melanie Rose D’Souza
Keyword(s):  
Oxidative Stress ◽  
Normal Control ◽  
Wistar Rats ◽  
Group Iv ◽  
Control Group ◽  
Methyl Gallate ◽  
Group Iii ◽  
Group I ◽  
Female Wistar Rats ◽  
Group Ii

Doxorubicin-induced cardiotoxicity is the leading cause of morbidity and mortality among cancer survivors. The present study was aimed to investigate the cardioprotective potential of methyl gallate; an active polyphenolic nutraceutical, against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats (150–200 g) were divided into four groups (n = 6) which consist of normal control (group I), doxorubicin control (group II), test-A (group III), and test-B (group IV). Group III and group IV animals were prophylactically treated with methyl gallate 150 mg/kg/day and 300 mg/kg/day orally, respectively, for seven days. Doxorubicin (25 mg/kg; single dose) was administered through an intraperitoneal route to group II, III, and IV animals on the seventh day to induce acute cardiotoxicity. On the 8th day, besides ECG analysis, serum CK, CK-MB, LDH, AST, MDA, and GSH were assayed. Following gross examination of isolated hearts, histopathological evaluation was performed by light microscopy. A significant ( p  < 0.05) cardiac injury, as well as oxidative stress, was observed in doxorubicin control rats in comparison to normal control rats. Methyl gallate at both the doses significantly ( p  < 0.05) reduced doxorubicin-induced ECG changes, dyslipidaemia, and elevation of CK, CK-MB, LDH, AST, MDA and increased GSH level. Methyl gallate reversed the doxorubicin-induced histopathological changes in the heart. The present study revealed that methyl gallate exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats by suppressing oxidative stress. Our study opens the perspective to clinical studies for consideration of methyl gallate as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

scholarly journals Haematological Study on the Effects of Goko Cleanser (Herbal Mixture) on Adult Female Wistar Rats

2021 ◽  
pp. 8-19
Author(s):  
Darlington Nnamdi Onyejike ◽  
McWilliams, Winifred Chidera ◽  
Mmaju, Chidinma Ifeyinwa ◽  
Okeke, Somadina Nnamdi ◽  
Obiesie, Ifechukwu Justicia ◽  
...  
Keyword(s):  
Adult Female ◽  
Wistar Rats ◽  
The Body ◽  
Control Group ◽  
Herbal Mixture ◽  
Female Wistar Rats ◽  
Group 3 ◽  
Haematological Study ◽  
The Brain ◽  
Herbal Mixtures

Introduction: Goko cleanser is one of the popular herbal mixtures used by the Nigerian populace. This study was carried out to evaluate the effects of this herbal mixture on some haematological parameters of adult female Wistar rats. Methodology: A total of twenty five Wistar rats weighing about 160 – 280g were divided into five groups according to their corresponding weights. The groups were designated as groups 1 – 5. Group 1 served as the control group, while groups 2, 3, 4, and 5 received 1000mg/Kg, 1500mg/Kg, 2000mg/Kg and 4000mg/Kg respectively. Results: The TWBC, PCV, PLATELET count, and RBC and Hb showed a statistically significant (p<0.05) increase for the test groups 2 – 5 when compared to the control group. The body weight had a statistically significant (p<0.05) decrease in groups 2, 4, and 5; while group 3 had a statistically insignificant (p>0.05) decrease. Conclusion and Recommendation: This study revealed that Goko Cleanser contains some haemopoietic benefits, but inhibit appetite centres. Hence, this study recommends that further study be carried out on the effects of this herbal mixture on the brain so as to reveal the extent of its effect on the appetite centres.

scholarly journals Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome

2015 ◽  
Vol 6 (2) ◽  
pp. 67-72
Author(s):  
Manizheh Karami ◽  
Fatemeh Lakzaei ◽  
MohammadReza Jalali Nadoushan
Keyword(s):  
Nitric Oxide ◽  
Polycystic Ovary Syndrome ◽  
Wistar Rats ◽  
Polycystic Ovary ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Ovary Syndrome ◽  
Crown Rump Length ◽  
Female Wistar Rats

ABSTRACT Background and objective Polycystic ovary syndrome (PCOS) can be induced in Wistar rats by over production of nitric oxide (NO). This study evaluated the efficacy of naloxone on the breeding characteristics of rats suffering from nitric oxide induced PCOS. Materials and methods Twenty-four female Wistar rats(200–250 gm) were kept as virgin under standard conditions. They were divided into four groups (n = 6). One group of the animals received L-arginine (50 mg/kg) intraperitoneally (i.p.) for 9 days/once a day. Another group was administered naloxone hydrochloride (0.4 mg/kg, i.p.) prior to injection of L-arginine. The third group was injected solely naloxone. Control group received saline solution (1 ml/kg, i.p.). After the treatments, all female rats were coupled with the intact males. They were then separated by observation of vaginal plaques; it was considered as day 0 of pregnancy. Eventually, they were operated on days 18 to 19 of the gestation to collect the animals’ ovaries. The samples were studied for pathological evidence. The fetal number and weight along with the fetal crown-rump length (CRL) were measured. Results The ovaries obtained from the L-arginine treated group had large cysts with thickened granulosa cell layer in contrast to those of the control or naloxone treated rats (p < 0.0001). The number of fetus though showed a decrease in the L-arginine treated rats (3 ± 1), but the fetal weight or fetal CRL did not change (p > 0.05). Conclusion This study may clearly illustrate the polycystic characteristics in the L-arginine treated group. It may particularly display the breeding efficacy of naloxone in rats with PCOS. How to cite this article Karami M, Lakzaei F, Nadoushan MRJ. Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome. Int J Infertil Fetal Med 2015;6(2):67-72.

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