Ameliorative Effect of Trevo Dietary Supplement Against Lead Acetate Nephrotoxicity

Background: Trevo is a nutritional supplement with numerous bioactive natural products, with detoxifying and antioxidant properties. The purpose of this study was to investigate the ability of Trévo to protect against oxidative stress induced by lead in the kidneys of male Wistar rats. Methods: Thirty-five healthy male Wistar rats were divided into five groups of seven rats each, using a randomized design. I=control; II=15 mg/kg of lead acetate (PbA); III=2 ml/kg of trevo+PbA; IV=5 ml/kg of trevo+PbA; V=5 ml/kg of trevo. Animals were treated with trevo for five days before co-administration with lead intraperitoneally for 10 consecutive days. Animals were sacrificed 24hr after the last administration, blood samples were collected via cardiac puncture, and processed for assessment of urea, creatinine, and uric acid (UA), while the kidney samples were excised and processed for the following biochemical assays: Malondialdehyde (MDA), Glutathione-S-Transferase (GST), Catalase (CAT), Superoxide Dismutase (SOD), and Reduced Glutathione (GSH). Results: Injection of PbA caused a significant increase in the serum levels of urea, creatinine, and uric acid, and a significant increase (P<0.001) in the MDA concentration, and decreases in GSH concentration, CAT, SOD, and GST activities (P<0.05) as compared to the controls. Pretreatment with trevo prevented the oxidative stress induced by lead acetate in the kidney tissue samples and improve the renal function. The protective effect was evident at 5 ml/kg of trevo. Conclusion: The results showed that trevo was nephroprotective against lead toxicity and the activity might be linked to the presence of numerous antioxidant phytochemicals present in trevo.

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Adansonia digitata L. leaf extract attenuates lead-induced cortical histoarchitectural changes and oxidative stress in the prefrontal cortex of adult male Wistar rats

Drug Metabolism and Personalized Therapy ◽

10.1515/dmdi-2020-0116 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Vivian Atuadu ◽

Ben-Azu Benneth ◽

John Oyem ◽

Emmanuel Esom ◽

Chris Mba ◽

...

Keyword(s):

Oxidative Stress ◽

Prefrontal Cortex ◽

Lead Acetate ◽

Wistar Rats ◽

Leaf Extract ◽

Neurological Diseases ◽

Lead Toxicity ◽

Adansonia Digitata ◽

Male Wistar Rats ◽

Aqueous Leaf Extract

AbstractObjectivesAdansonia digitata L. is popularly known for the management of various neurological diseases in ethno-medicine. Studies have shown that lead toxicity is a possible risk factor for early onset of neurodegenerative disease. Hence, this study was designed to evaluate the effect of A. digitata aqueous leaf extract (ADALE) against lead-induced oxidative stress and histo-architectural changes in the prefrontal cortex of adult Wistar rats.MethodsSaline (10 mL/kg), ADALE (500 and 1000 mg/kg) and EDTA (55 mg/kg) were pretreated orally 30 min prior to lead acetate (LA) (120 mg/kg) administration to male Wistar rats (n=7) for 21 days. Thereafter, standard biochemical (superoxide dismutate, catalase, glutathionxe and malondialdehyde), histological (H&E) and histochemical assessment (crystyl fast violet stain for nissil substance) were carried out in the prefrontal cortex.ResultsADALE significantly (p<0.05) reversed LA-induced oxidative stress, as evidenced by increased catalase, superoxide dismutase and oxidized glutathione levels, and decreased malondialdehyde concentration in the prefrontal cortex. Also, the increase chromatolysis and neuronal pyknosis of the pyramidal neurons of the prefrontal cortex were significantly attenuated by ADALE.ConclusionsThe result of this study showed that A. digitata aqueous leaf extract attenuated lead acetate-induced cortical neurodegeneration via inhibition of oxidative stress.

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Honey prevents neurobehavioural deficit and oxidative stress induced by lead acetate exposure in male wistar rats- a preliminary study

Metabolic Brain Disease ◽

10.1007/s11011-015-9733-6 ◽

2015 ◽

Vol 31 (1) ◽

pp. 37-44 ◽

Cited By ~ 19

Author(s):

Wahab Imam Abdulmajeed ◽

Habeeb Bolakale Sulieman ◽

Maymunah Oloruntosin Zubayr ◽

Aminu Imam ◽

Abdulbasit Amin ◽

...

Keyword(s):

Oxidative Stress ◽

Lead Acetate ◽

Wistar Rats ◽

Male Wistar Rats ◽

Preliminary Study ◽

And Oxidative Stress

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Comparative Nephrotoxicity and Hepatotoxicity Effects of Kerosene, Gasoline, Liquefied Petroleum Gas and Biomass Fuel Exposure on Male Albino Rats

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2020/v7i430147 ◽

2020 ◽

pp. 45-52

Author(s):

Ude Tochukwu ◽

Meludu Samuel Chukwuemeka ◽

Dioka Chudi Emmanuel ◽

Chikezie Onyebuchi Desmond ◽

Awalu Chimezie Joseph ◽

...

Keyword(s):

Uric Acid ◽

Wistar Rats ◽

Normal Liver ◽

Serum Levels ◽

Biomass Fuel ◽

Whole Body ◽

Albino Rats ◽

Liquefied Petroleum Gas ◽

Fuel Wood ◽

Male Wistar Rats

The effects of Kerosene, gasoline, and liquefied petroleum gas and biomass fuel exposure on biomarkers of kidney and liver were investigated in male wistar rats. Fifty adult male wistar rats were randomly assigned to five groups of ten animals each. Rats in group A served as control (exposed to fresh air). Group B, C, D and E were exposed to inhalation of kerosene, gasoline, liquefied petroleum gas and biomass fuel (wood smoke) respectively. All the exposures were done using whole body exposure chambers 70 cm x 60 cm x 60 cm measurement for six weeks, 6 days per week. Five millilitres of blood sample were collected and serum extracted at the end of six weeks. The serum concentration of urea, creatinine, uric acid and activities AST, ALT, γGT were determined using Cobas reagent kits manufactured by Roche Diagnostics GmbH, Sandhofer Strasse 116, D-68305 Mannheim, Germany. Values were analysed statistically using SPSS version 23.0. The result shows significant increase in the serum levels of urea, creatinine and uric acid of test groups relative to control (p<0.05), though the effect appear to be more pronounced with exposure to kerosene, gasoline and biomass fuel. The exposure also led to significant increase in activities of AST, ALT and γGT (p<0.05). These results suggest that repeated exposure to kerosene, gasoline and liquefied petroleum gas and biomass fumes may elicit hepatic and renal toxicity, thereby impairing the normal liver and kidney function.

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Effects of Aqueous Extract of Ocimum gratissimum Leaves and Vitamin C on Lead Acetate-induced Changes in the Thymus of Adult Wistar Rats

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2019/v26i130087 ◽

2019 ◽

pp. 1-9

Author(s):

G. N. Okechukwu ◽

E. Ezor ◽

E. Finbarrs-Bello ◽

L. N. Ebube ◽

G. C. Uzomba ◽

...

Keyword(s):

Vitamin C ◽

Aqueous Extract ◽

Lead Acetate ◽

Wistar Rats ◽

Lead Toxicity ◽

Wistar Rat ◽

Ocimum Gratissimum ◽

Ameliorative Effect ◽

Male Wistar Rats ◽

Induced Changes

Lead is a heavy metal found in earth’s crust. It is a widespread and insidious environmental toxin known as a severe and aggressive contaminant to human and animal organisms’ health status. This work is aimed at evaluating the effect of aqueous extract of Ocimum gratissimum (OG) on lead induced changes in the thymus of adult albino wistar rats. Thirty five male Wistar rats were used in the study and were randomly divided into seven groups with five rats in each group. The rats in Group one (G1) served as the Control and received distilled water. Group 2 received 120 mg/kg body weight (bwt) of lead acetate, G3 received 375 mg/kg bwt of OG only. G4 received 120 mg/kg bwt of lead acetate and OG extract at 375 mg/kg bwt., G5 received 120 mg/kg bwt of lead and OG at 750 mg/kg bwt. G6 received 375 mg/kg bwt of OG in two weeks followed by 120 mg/kg bwt of lead acetate for one week, while G7 received 120 mg/kg bwt of lead acetate and Vitamin C at 11900 mg/kg. All the administrations were carried out orally for twenty one (21) days. At the end of the administration, the rats were fasted for 24 hours. They weighted and humanely sacrificed via cervical dislocation. The thymus were harvested in all the groups and prepared for histological studies using routine haematoxylin and eosin (H&E) staining techniques. The result of the present study shows that lead possessed a distortive effect in the histoarchitecture of thymus of the wistar rat in G2 when compared with G1. Treatment of these experimental animals with vitamin C prove to posses more ameliorative effect in restoring the histoarchitecture on lead toxicity in the thymus closed to normal than OG. Therefore, it is suggested that aqueous extract OG can act via the same pathway as vitamin C, in maintaining the normal histological structures of the thymus of adult albino wistar rat exposed to lead toxicity.

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Oxidative stress and antioxidant status in rotenone induced rat models of Parkinson’s disease

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i1.1776 ◽

2020 ◽

Vol 11 (1) ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Suchitra Kavuri ◽

Senthilkumar Sivanesan ◽

Vijayaraghavan Rajagopalan

Keyword(s):

Oxidative Stress ◽

Parkinson’S Disease ◽

Parkinson's Disease ◽

Wistar Rats ◽

Scientific Evidence ◽

Antioxidant Properties ◽

Treatment Modalities ◽

Study Results ◽

Male Wistar Rats ◽

Antioxidant Markers

Parkinson disease (PD) is involved in the damage of neurons of the brain that secretes dopamine. Understanding the mechanisms involved in Parkinson's disease helps to develop effective management methods. Hence, the present work aimed to unravel to observe the variations, if any, in the parameters of oxidative stress and antioxidant markers between oral and ip rotenone-induced rat model of Parkinson’s disease. Male Wistar rats weighing between 250-300g were housed in solid bottomed polypropylene cages under strict veterinary supervision and maintained in rooms with 12hrs light / dark cycle. The randomly selected male Wistar rats were equally divided into four groups, with six rats in each group. The study results provide scientific evidence for marked changes in both the oxidative and anti-oxidant parameters, followed by the ip and po rotenone administration. It is the need of the time to consider the neuroprotective and antioxidant properties while developing the newer treatment modalities for the management of PD.

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Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2020-0281 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Olugbemi T. Olaniyan ◽

Olakunle A. Ojewale ◽

Ayobami Dare ◽

Olufemi Adebayo ◽

Joseph E. Enyojo ◽

...

Keyword(s):

Luteinizing Hormone ◽

Lead Acetate ◽

Wistar Rats ◽

Histological Analysis ◽

Cocos Nucifera ◽

Reproductive Toxicity ◽

Positive Control ◽

Negative Control ◽

Male Wistar Rats ◽

Cocos Nucifera L

Abstract Objectives Lead primarily affects male reproductive functions via hormonal imbalance and morphological damage to the testicular tissue with significant alteration in sperm profile and oxidative markers. Though, different studies have reported that Cocos nucifera L. oil has a wide range of biological effects, this study aimed at investigating the effect of Cocos nucifera L. oil on lead acetate-induced reproductive toxicity in male Wistar rats. Methods Twenty (20) sexually matured male Wistar rats (55–65 days) were randomly distributed into four groups (n=5). Group I (negative control)—distilled water orally for 56 days, Group II (positive control)—5 mg/kg bwt lead acetate intraperitoneally (i.p.) for 14 days, Group III—6.7 mL/kg bwt Cocos nucifera L. oil orally for 56 days and Group IV—lead acetate intraperitoneally (i.p.) for 14 days and Cocos nucifera L. oil for orally for 56 days. Rats were sacrificed by diethyl ether, after which the serum, testis and epididymis were collected and used for semen analysis, biochemical and histological analysis. Results The lead acetate significantly increases (p<0.05) testicular and epididymal malondialdehyde (MDA) levels, while a significant reduction (p<0.05) in sperm parameters, organ weight, testosterone and luteinizing hormone was observed when compared with the negative control. The coadministration of Cocos nucifera oil with lead acetate significantly increases (p<0.05) testosterone, luteinizing hormone, sperm parameters and organ weight, with a significant decrease (p<0.05) in MDA levels compared with positive control. Histological analysis showed that lead acetate distorts testicular cytoarchitecture and germ cell integrity while this was normalized in the cotreated group. Conclusions Cocos nucifera oil attenuates the deleterious effects of lead acetate in male Wistar rats, which could be attributed to its polyphenol content and antioxidant properties.

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Aortic-banding induces myocardial oxidative stress and changes in concentration and activity of antioxidants in male Wistar rats

Life Sciences ◽

10.1016/j.lfs.2006.07.015 ◽

2006 ◽

Vol 79 (23) ◽

pp. 2187-2193 ◽

Cited By ~ 16

Author(s):

Maria H.V.M. Jacob ◽

Mauro R.N. Pontes ◽

Alex S.R. Araújo ◽

Jaqueline Barp ◽

Maria C. Irigoyen ◽

...

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Aortic Banding ◽

Myocardial Oxidative Stress ◽

Male Wistar Rats

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Effect of different types of stress on adrenal gland parameters and adrenal hormones in the blood serum of male Wistar rats

Archives of Biological Sciences ◽

10.2298/abs0902187a ◽

2009 ◽

Vol 61 (2) ◽

pp. 187-194 ◽

Cited By ~ 3

Author(s):

M. Adzic ◽

Ana Djordjevic ◽

Jelena Djordjevic ◽

Ana Niciforovic ◽

Marija Radojcic

Keyword(s):

Blood Serum ◽

Wistar Rats ◽

Adrenal Glands ◽

Acute Stress ◽

Serum Levels ◽

Combined Stress ◽

Adrenal Hormones ◽

General Adaptation ◽

Different Types ◽

Male Wistar Rats

In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamine, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.

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