scholarly journals Study of the resistance of cows with mastitis depending on the age of lactation

2022 ◽  
Vol 354 (11-12) ◽  
pp. 27-31
Author(s):  
M. V. Dovydenkova
Keyword(s):  
Blood Serum ◽  
Immune Status ◽  
Animal Husbandry ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
The Body ◽  
Nonspecific Resistance ◽  
Animal Blood ◽  
Black And White ◽  
Holstein Breed

Relevance. The breeding stock and young cattle in the conditions of industrial technology of animal husbandry experience an increased antigenic load, which leads to disturbances in the immune status and the development of inflammatory processes manifested by endometritis and mastitis, diarrheal and respiratory syndromes. It was relevant to conduct a comprehensive assessment of the immunological reactivity of the animal’s body according to the state of resistance in cattle with clinical and subclinical mastitis, depending on the age of lactation. As a result of complex studies, the specific and nonspecific reactivity of the body of cattle in different periods of lactation was studied.Methods. The object of the study were cows of a black-and-white Holstein breed (n = 450). Groups of animals were formed according to the age of lactation:1st lactation, 2–3rd lactation, 3–4th lactation and for health reasons: clinically healthy animals, with subclinical mastitis and clinical mastitis. Milk samples were taken from cows to count the number of somatic cells, and blood to determine the quantitative content of IgG immunoglobulin and indicators of nonspecific resistance.Results. It was found that with mastitis in cows of different lactation ages, almost identical changes in the immune status were observed. Quantitative determination of IgG levels in animal blood serum is the basis for assessing the immunity of cattle. The immune status in clinically healthy animals was characterized by a higher level of the total amount of IgG immunoglobulin in the blood serum (amounted to 22.25 ± 0.70 mg/ml), whereas in cows with a subclinical form of mastitis, the level of IgG immunoglobulin decreased by 12.3%, with a clinical form of mastitis — by 17.2%. The results also showed that in cows of different lactations with subclinical and clinical mastitis, the bactericidal activity (by 10.8%) and the lysozyme activity of the blood increased significantly (by 8.22%). In cows with clinical mastitis — by 8.6% and 9.7% respectively, compared with the indicators in groups of healthy animals. Thus, mastitis, occurring in acute and subacute form, leads to a decrease in the resistance of the cows’ organism.

scholarly journals REASIL HUMICVET AND REASIL HUMIC HEALTH SUPPLEMENTS BASED ON HUMIC ACIDS IN THE DIETS OF DAIRY CALVES

2021 ◽  
Vol 6 (1) ◽  
pp. 64-70
Author(s):  
Andrei Frolkin ◽  
Haidar Valitov ◽  
Aleksandr Varakin ◽  
Valentina Kornilova
Keyword(s):  
Blood Serum ◽  
Live Weight ◽  
Mineral Elements ◽  
The Body ◽  
Control Group ◽  
Liquid Form ◽  
Daily Growth ◽  
Black And White ◽  
Significant Difference ◽  
Experimental Group

The purpose of the research is to increase the effectiveness of the supplement Reasil on the growth of milk-fed calves. For the scientific and economic experience, three groups of calves were formed (control, 1 and 2 experi-enced). Groups of animals of 10 heads were formed according to the principle of analogues. The indicators of live weight, growth and blood of calves were studied after Reasil use in a diet: Reasil HumicVet as a liquid form, and Reasil Humic Health – reduced to powder. In the experiment, animals of a black-and-white breed were used. The Reasil feeding in the diet contributed to an increase in the live weight of milk-fed calves in the 1-st and the 2-nd experimental groups at two months of age by 5.65 and 5.48 kg (or by 7.4 and 7.2%), respectively, compared to the indicator of the control group analogues. The average daily growth of calves of the experimental groups exceeded this indicator of control animals by 96 and 82 g, respectively. The blood of calves of the 1st and 2nd experimental groups, showed that hematoglobin was higher by 15.7 and 11.4%, red blood cells – by 16.5 and 15.1%, and the alkaline reserve – by 5.2%, compared with blood of calves from the control group, which indicates the activation of metabolic processes in the body. The total protein amount in the blood serum of animals from the experimental groups increased by 8.4 and 5.9% respectively, compared to the tests of calves from the control group. The albu-min content of animals of the 1st experimental group tested was higher by 5.3%; from the 2nd experimental group there was no significant difference compared to the control. The number of gamma-globulins in the blood of ani-mals of the experimental groups increased, which indicates an increase in defense reaction of animals. The con-tent of calcium in the blood serum of calves from the experimental groups was higher by 8.3 and 5.9%, phosphorus – by 4.8 and 2.4%, which indicates a more effective use of these mineral elements.

scholarly journals Formation features of humoral immunity in newborn calves in case of including haylage with bio-preservative into the diet of cows

2021 ◽  
Vol 273 ◽  
pp. 02008
Author(s):  
Anna Karamaeva ◽  
Larisa Bakaeva ◽  
Natalia Soboleva ◽  
Sergey Karamaev
Keyword(s):  
Blood Serum ◽  
Milk Yield ◽  
Humoral Immunity ◽  
Immune Status ◽  
The Body ◽  
Group Iii ◽  
First Feeding ◽  
Group I ◽  
Newborn Calves ◽  
Group Ii

The peculiarities of the formation of colostrum in the udder of cows and the immune status in the body of calves in the first hours and days after birth, when included in the diet of cows, haylage from eastern goat’s rue prepared with the use of bio-preservatives “Silostan” (group II) and “GreenGrass 3 × 3”(Group III). The study of colostrum of the first milk yield showed that feeding cows with silage with the bio-preservative “Silostan” contributed to an increase in the content of immunoglobulins by 4.0% (Р<0,001), with bio-preservative “GreenGras 3 × 3” - by 5.3% (Р<0,001). After drinking the first portion of colostrum, immunoglobulins appear in the blood serum of calves after 2 hours. After 6 hours, the content of immunoglobulins in the blood serum of calves of group II was 21.9% higher than in group I (haylage without preservative) (P < 0.05), group III - by 27.1% (P <0.001). The proportion of calves with an immunoglobulin content 6 hours after the first feeding with colostrum not more than 6.0 mg / ml, decreased in group II by 12.0%, in group III - by 20.0%.

scholarly journals THE EFFECT OF «ARCUSIT» ON BIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS BLOOD SERA IN SILVER-BLACK FOX (SILVER-BLACK FOX)

2021 ◽  
Vol 247 (3) ◽  
pp. 192-197
Author(s):  
I.I. Okulova ◽  
◽  
I.A. Domsky ◽  
Yu.A. Berezina ◽  
Z.N. Beltyukova ◽  
...  
Keyword(s):  
Experimental Data ◽  
Blood Serum ◽  
Bactericidal Activity ◽  
Lysozyme Activity ◽  
The Body ◽  
Phagocytic Index ◽  
Control Group ◽  
Immunological Parameters ◽  
Nonspecific Resistance ◽  
Experimental Group

The use of «Arkusit» at a dose of 20 μg / kg of body weight indicates a beneficial effect on the factors of nonspecific resistance of the silver-black fox. The concentration of alkaline phosphatase in the blood serum in the experimental group decreased by 1.8 times (P <0.001), the ALT index - by 1.2 (P <0.05). The concentration of glucose in the experimental group increased 2 times (P <0.05) compared with the control group. In animals of the experimental group, the lysozyme activity of blood serum increased 1.3 times (P <0.05), the OPR reaction and the phagocytic index - 1.4 times (P <0.05), the bactericidal activity of blood serum - 1.8 times (P <0.05). «Arcusit» stimulates the synthesis of gamma globulins, increases the opsonophagocytic, bactericidal activity of blood serum. Thus, the analysis of experimental data indicates that the drug «Arcusit» has a pronounced immunostimulating effect, providing the activation of factors of nonspecific resistance of the body of the silver-black fox.

scholarly journals AQUEOUS EXTRACT OF RED TEA PLANT (Hibiscus sabdariffa L) EFFECT ON THE LIPID PROFILE AND GLUGOSE IN MALE WHITE RABBITS EXPOSED TO OXIDATIVE STRESS

2016 ◽  
Vol 47 (1) ◽  
Author(s):  
S. O. Al-khateeb
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Blood Serum ◽  
Lipid Profile ◽  
Aqueous Extract ◽  
High Density ◽  
The Body ◽  
High Density Lipoproteins ◽  
Animal Blood ◽  
Atherogenic Indices

This study aimed to investigate the of effect aqueous extract of red tea on average of glucose and lipid profile in New Zealand white male rabbits exposed to oxidative stress induced hydrogen peroxide H2O2 of drinking water for the duration 30-days. Treatment of  hydrogen peroxide 2% led alone to a significant increase (P≤0.05) in the concentration of glucose level and the concentration of total cholesterol rate and level of triglycerides and a significant decrease (P ≤ 0.05) in the level of estimating the concentration of high-density cholesterol in the blood serum lipoproteins and a significant increase in the concentration of lipoproteins and very low-lying density cholesterol was significantly higher in atherogenic indices the first and second, respectively, in the blood of animals treated with hydrogen peroxide compared to the control group. It got a significant decrease (P≤0.05) in the concentration of glucose and cholesterol rate and triglycerides and in the concentration of lipoproteins and sessile very density cholesterol and in the atherogenic indices first and second respectively, as happened morally rise in the level of estimating the concentration of high-density lipoproteins cholesterol in the labor animal blood serum aqueous extract of red tea (125 mg / kg of body weight) added all of the hydrogen peroxide compared with the group treated rabbits hydrogen peroxide alone. It is concluded from this current study, the important role of the Red tea as an antioxidant through curb the harmful effects of some types of free radicals within the body and thus improve the most changes biochemical changes in the male albino rabbits  after exposure to oxidative stress Induced by hydrogen Peroxide 2% .

METABOLIC STATUS OF THE ORGANISM OF BULLS-PRODUCERS OF DIFFERENT GENOTYPES

2020 ◽  
Author(s):  
Н.В. БОГОЛЮБОВА ◽  
Р.А. РЫКОВ
Keyword(s):  
Blood Serum ◽  
Mineral Metabolism ◽  
The Body ◽  
Holstein Breed ◽  
Blood Biochemical ◽  
Health And Nutrition ◽  
Biochemical Status ◽  
Comparative Aspect ◽  
Holstein Bulls ◽  
Blood Biochemical Indicators

Изучили биохимический статус организма быков-производителей голштинской (n=8) и симментальской (n=8) пород в сравнительном аспекте. Проанализированы пробы сыворотки крови быков-производителей, содержащихся в условиях племпредприятия Московской области. В образцах крови определяли показатели азотистого, углеводно-липидного, минерального обменов, активность ферментов. Содержание общего белка и его фракций, мочевины, а также соотношение альбуминов и глобулинов были примерно на одном уровне независимо от генотипа животных. В крови быков симментальской породы, по сравнению с аналогами голштинской породы, была выше концентрация креатинина на 19,9% (P<0,05), уровень фосфолипидов — на 16%, холестерин — на 46,5% (P<0,05). Концентрация фосфора в сыворотке крови симментальских быков составляла 2,52 мМ/л, что на 30,5% больше, чем у голштинов (P<0,05). Отношение кальция к фосфору в организме симментальских быков равнялось 1,04 против 1,40 ед. в группе голшинских быков. Полученные данные указывают на различия в протекании азотистого, углеводно-липидного и минерального обменов в организме животных мясо-молочного и молочного направлений продуктивности и помогут в разработке эталонных значений биохимических показателей крови для оценки здоровья и уровня питания быков-производителей изучаемых пород. We studied the biochemical status of the organism of Holstein (n=8) and Simmental (n=8) bulls-producers in a comparative aspect. Samples of blood serum of bulls-producers contained in the conditions of the Moscow region were analyzed. Indicators of nitrogen, carbohydrate-lipid, mineral metabolism, and enzyme activity were determined in the blood samples. The content of total protein and its fractions, urea, as well as the ratio of albumins and globulins were approximately at the same level, regardless of the genotype of animals. In the blood of Simmental bulls, compared with analogues of the Holstein breed, the concentration of creatinine was higher by 19.9% (P<0.05), the level of phospholipids — by 16%, and cholesterol — by 46.5% (P<0.05). The concentration of phosphorus in the blood serum of Simmental bulls was 2.52 mmol/l, which is 30.5% higher than in Holsteins bulls (P<0.05). The ratio of calcium to phosphorus in the body of Simmental bulls was 1.04 against 1.40 units. in the Holstein bulls. The obtained data indicate differences in the nitrogen, carbohydrate-lipid and mineral metabolism in the body of animals of meat-and-milk and dairy productivity and will help in the reference values of blood biochemical indicators development for assessing the health and nutrition level of bulls-producers of the studied breeds.

scholarly journals Review : Kejadian mastitis dan kaitannya dengan vitamin dan Trace Mineral Cu, Zn, Se

2018 ◽  
Vol 28 (3) ◽  
pp. 265
Author(s):  
Yanuartono - Yanuartono ◽  
Alfarisa Nururrozi ◽  
Soedarmanto Indarjulianto ◽  
Hary Purnamaningsih ◽  
Nurman Haribowo
Keyword(s):  
Free Radicals ◽  
Dairy Cows ◽  
Immune Status ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Common Disease ◽  
Protective Effects ◽  
Trace Mineral ◽  
Β Carotene

<p>Mastitis means inflammation of the udder and is a common disease among dairy cows worldwide. It is subdivided into clinical mastitis and subclinical mastitis, both influence milk quality and quantity, and mastitis is therefore of major economic concern for the farmer. The incidence rate of mastitis varies between herds and depends on the exposure to pathogens, environmental and management factors and the immune status of the cow. Mastitis is associated with release of free radicals, increased total oxidant capacity and decreased total antioxidants capacity in milk. Vitamin C, E, , α tocopherol, A, β-karoten and trace mineral (Cu, Zn, and Se) feeding, as an antioxidant, in dairy cows have shown appreciable protective effects by reducing the incidence of mastitis.The purpose of this review is to discuss the role of Vitamins C, E, α tocopherol, A, β-carotene, Cu, Zn, and Se in the incidence of mastitis.</p>

scholarly journals BACTERICIDAL ACTIVITY OF A SANITARY AND HYGIENIC PRODUCT BASED ON BACILLUS SUBTILIS BACTERIA STRAINS

2020 ◽  
Vol 99 (3) ◽  
pp. 265-269
Author(s):  
Nadezda P. Tarabukina ◽  
M. P. Neustroev ◽  
A. M. Stepanova ◽  
S. I. Parnikova ◽  
S. V. Dulova ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bactericidal Activity ◽  
Bacterial Contamination ◽  
Animal Husbandry ◽  
Subclinical Mastitis ◽  
The Body ◽  
Bacterial Strains ◽  
Anaerobic Microorganisms ◽  
Dairy Cattle Breeding ◽  
Permafrost Soils

Introduction. A possible alternative to traditional methods of the treatment with chemical disinfectants are effective and safe preparations based on strains of bacteria Bacillus subtilis. The development of effective hygiene products based on Bacillus subtilis bacteria strains for air sanitation, household items, and rooms that do not adversely affect the body in the presence of humans, animals, and birds is an urgent task of sanitation and hygiene. Material and methods. Suspensions of an equal combination of the bacterial strains Bacillus subtilis TNP-3 and Bacillus subtilis TNP-5 were added to a solution of bicarbonate and sodium chloride. The strains were isolated from the permafrost soils of Yakutia, certified and deposited in the All-Russian collection of microorganisms used in animal husbandry and veterinary medicine (VGNKI, Moscow). The bactericidal activity of the «Probiodes 3 + 5» solutions was studied by the method of “coarse calico tests”. As test cultures there was used 2 billion suspension of diurnal cultures of Str. equi H-34 and Sal. abortus equi BN-12. The effectiveness of sanitization was taken into account according to the level of bacterial contamination - the number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAnM) on the skin of the nipples of the udder. Results. Reliable disinfection of coarse calico tests contaminated with Str. equi H-34, was established with the use of «Probiodez 3 + 5» (5x10 8 CFU / ml) for 6 hours exposure. By increasing the dose of B. subtilis in the solution «Probiodes 3 + 5» to (1.0 × 109 CFU / ml), there was reliable disinfection of coarse calico tests contaminated with Sal. abortus equi BN-12, exposure 6 hours. The drug «Probiodes 3 + 5» helps to reduce the total bacterial contamination and subclinical mastitis, effective for sanitary-hygienic treatment in dairy cattle breeding. Conclusion. The results of the studies allow making a conclusion that the bacterial strains B. subtilis TNP-3 and B. subtilis TNP-5 are promising for the development of effective, safe hygienic and sanitizing agents for widespread use.)

scholarly journals Assessment of the Impact of Betulin on the Immune Status of Cows with Leukemia-Associated Infection

2021 ◽  
Author(s):  
Vasily Vlasenko ◽  
Valentina Pleshakova ◽  
Sayat Bayseitov ◽  
Nadezhda Lescheva
Keyword(s):  
Metabolic Activity ◽  
Immune Status ◽  
Leukemia Virus ◽  
Effective Means ◽  
The Body ◽  
Lymphoid Cells ◽  
Serum Samples ◽  
Nonspecific Resistance ◽  
Serological Studies ◽  
The Impact

This study examined the immunity dynamics of bovine brucellosis-complicated leukemia, as well as the efficacy of treatment with betulin. Blood serum samples were taken from cows with specific brucellosis antibodies and bovine leukemia virus antibodies based on the results of complex diagnostic studies. Animals (n = 5) selected based on serological studies were injected subcutaneously with betulin-PEG three times with an interval of 7 days at a dose of 10 mg/kg. Blood was taken for hematological and immunological studies before the injection, and after the first, second and third injections. It was found that leukemia and brucellosis infections were accompanied by a significant increase in the number of leukocytes and lymphoid cells, as well as strong fluctuations in the leukocyte functional and metabolic activity parameters (from insignificant in some individual animals to significantly increased in others). Immunological restructuring of the body after triplicate administration of betulin-PEG was characterized by the restoration of the number of lymphoid cells to the level of clinically healthy animals, as well as a significant increase in the functional and metabolic activity of the neutrophilic granulocytes, especially their oxygen-dependent systems. Based on the results, the product is recommended as an effective means to restrain the leukemia process that is complicated with associated infection, as well as to use for seronegative animals in order to increase nonspecific resistance to BLV infection. Keywords: leukemia, brucellosis, cattle, immune status, betulin, immunocorrection, associated infection

scholarly journals Bactericidal activity of a sanitary and hygienic product based on Bacillus subtilis bacteria strains

2020 ◽  
Vol 99 (3) ◽  
pp. 265-269
Author(s):  
Надежда Петровна Тарабукина ◽  
М.П. P. Неустроев ◽  
А.М. M. Степанова ◽  
С.И. I. Парникова ◽  
С.В. V. Дулова ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bactericidal Activity ◽  
Bacterial Contamination ◽  
Animal Husbandry ◽  
Subclinical Mastitis ◽  
The Body ◽  
Bacterial Strains ◽  
Anaerobic Microorganisms ◽  
Dairy Cattle Breeding ◽  
Permafrost Soils

Introduction. A possible alternative to traditional methods of the treatment with chemical disinfectants are effective and safe preparations based on strains of bacteria Bacillus subtilis. The development of effective hygiene products based on Bacillus subtilis bacteria strains for air sanitation, household items, and rooms that do not adversely affect the body in the presence of humans, animals, and birds is an urgent task of sanitation and hygiene. Material and methods. Suspensions of an equal combination of the bacterial strains Bacillus subtilis TNP-3 and Bacillus subtilis TNP-5 were added to a solution of bicarbonate and sodium chloride. The strains were isolated from the permafrost soils of Yakutia, certified and deposited in the All-Russian collection of microorganisms used in animal husbandry and veterinary medicine (VGNKI, Moscow). The bactericidal activity of the «Probiodes 3 + 5» solutions was studied by the method of “coarse calico tests”. As test cultures there was used 2 billion suspension of diurnal cultures of Str. equi H-34 and Sal. abortus equi BN-12. The effectiveness of sanitization was taken into account according to the level of bacterial contamination - the number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAnM) on the skin of the nipples of the udder. Results. Reliable disinfection of coarse calico tests contaminated with Str. equi H-34, was established with the use of «Probiodez 3 + 5» (5x10 8 CFU / ml) for 6 hours exposure. By increasing the dose of B. subtilis in the solution «Probiodes 3 + 5» to (1.0 × 109 CFU / ml), there was reliable disinfection of coarse calico tests contaminated with Sal. abortus equi BN-12, exposure 6 hours. The drug «Probiodes 3 + 5» helps to reduce the total bacterial contamination and subclinical mastitis, effective for sanitary-hygienic treatment in dairy cattle breeding. Conclusion. The results of the studies allow making a conclusion that the bacterial strains B. subtilis TNP-3 and B. subtilis TNP-5 are promising for the development of effective, safe hygienic and sanitizing agents for widespread use.)

scholarly journals Increase of Nonspecific Immunity and Productivity of Piglets when using the Probiotic Lactobacillus reuteri

2021 ◽  
pp. 121-127
Author(s):  
Ovcharova Anastasiya ◽  
Ostrenko Konstantin ◽  
Kutin Ivan
Keyword(s):  
High Efficiency ◽  
Immune Status ◽  
Lactobacillus Reuteri ◽  
The Body ◽  
Phagocytic Index ◽  
Control Group ◽  
Negative Consequences ◽  
Nonspecific Resistance ◽  
Weaning Piglets ◽  
Experimental Group

The stress of weaning piglets negatively affects all factors of the body. First of all, this affects the violation of nutrition and a decrease in the activity of the immune system. The use of probiotics allows to reduce the negative consequences caused by weaning from a sow. The aim of the study was to study the effect of probiotics based on Lactobacillus reuteri on the indicators of nonspecific resistance and productivity of piglets. The obtained data showed a high efficiency of the use of probiotics. In the experimental group, the phagocytic activity of neutrophils increased by 4.4%, the phagocytic index was 6.6% higher, and the bactericidal activity increased by 29% compared to the control group. In piglets treated with Lactobacillus reuteri, body weight increased by 9.3%, and the average daily gains in the control group increased by 15.8%. The use of probiotics in the period of stress after weaning allows you to activate the immune status.

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