Identification of non-tuberculosis mycobacteria isolated from cattle in the Republic of Tatarstan

The purpose of this work was to identify non-tuberculosism ycobacteria isolated from cattle in the Republic of Tatarstan. The article presents the results of identification of non-tuberculosis mycobacteria in samples of pathological material received from cattle reacting to tuberculin by polymerase chain reaction in real time and with electrophoretic detection. In the result of our research it is determined that in 43% of the explored samples of pathological material received from reacting to tuberculin cattle nontuberculous mycobacteria were identified.

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IDENTIFICATION OF NON-TUBERCULOSIS MYCOBACTERIA ISOLATED FROM ENVIRONMENTAL OBJECTS IN THE REPUBLIC OF TATARSTAN

Scientific Notes Kazan Bauman State Academy of Veterinary Medicine ◽

10.31588/2413-4201-1883-248-4-100-105 ◽

2021 ◽

Vol 248 (4) ◽

pp. 100-105

Author(s):

Y.R. Kamalieva ◽

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D.N. Mingaleev ◽

R.Kh. Ravilov ◽

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...

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Allergic Reactions ◽

Chain Reaction ◽

Species List ◽

Environmental Objects ◽

Polymerase Chain ◽

The Republic ◽

Bovine Tuberculin

The purpose of this work was to identify mycobacteria of non-tuberculosis type isolated from environmental objects in the Republic of Tatarstan. The article presents the result of identification of mycobacteria of non-tuberculous type in samples of washout from environmental objects obtained from farms with an identified species list of circulating mycobacteria of non-tuberculous type from reacting to tuberculin cattle by polymerase chain reaction in real time. In the course of our research, we found that nonspecific allergic reactions to bovine tuberculin in these farms are caused by contamination with mycobacteria of a non-tuberculosis type of livestock premises and soils near farms.

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The Use of Real-Time Polymerase Chain Reaction for Rapid Diagnosis of Skeletal Tuberculosis

Archives of Pathology & Laboratory Medicine ◽

10.5858/2006-130-1053-tuorpc ◽

2006 ◽

Vol 130 (7) ◽

pp. 1053-1056

Author(s):

Naomi Kobayashi ◽

Thomas G. Fraser ◽

Thomas W. Bauer ◽

Michael J. Joyce ◽

Gerri S. Hall ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Nontuberculous Mycobacteria ◽

Frozen Section ◽

Femoral Condyle ◽

Osteolytic Lesion ◽

Rapid Diagnosis ◽

Chain Reaction ◽

Skeletal Tuberculosis ◽

Polymerase Chain

Abstract We identified Mycobacterium tuberculosis DNA using real-time polymerase chain reaction on a specimen from an osteolytic lesion of a femoral condyle, in which the frozen section demonstrated granulomas. The process was much more rapid than is possible with culture. The rapid detection of M tuberculosis and the concomitant exclusion of granulomatous disease caused by nontuberculous mycobacteria or systemic fungi are necessary to appropriately treat skeletal tuberculosis. The detection and identification of M tuberculosis by culture may require several weeks using traditional methods. The real-time polymerase chain reaction method used has been shown to be rapid and reliable, and is able to detect and differentiate both tuberculous and nontuberculous mycobacteria. Real-time polymerase chain reaction may become a diagnostic standard for the evaluation of clinical specimens for the presence of mycobacteria; this case demonstrates the potential utility of this assay for the rapid diagnosis of skeletal tuberculosis.

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