362. An Evaluation of Procedures Affecting Fume Hood Performance

1999 ◽  
Author(s):  
D. Ghidoni
Keyword(s):  
Author(s):  
Jane Payne ◽  
Philip Coudron

This transmission electron microscopy (TEM) procedure was designed to examine a gram positive spore-forming bacillus in colony on various solid agar media with minimal artifact. Cellular morphology and organization of colonies embedded in Poly/Bed 812 resin (P/B) were studied. It is a modification of procedures used for undecalcified rat bone and Stomatococcus mucilaginosus.Cultures were fixed and processed at room temperature (RT) under a fume hood. Solutions were added with a Pasteur pipet and removed by gentle vacuum aspiration. Other equipment used is shown in Figure 3. Cultures were fixed for 17-18 h in 10-20 ml of RT 2% phosphate buffered glutaraldehyde (422 mosm/KgH2O) within 5 m after removal from the incubator. After 3 (30 m) changes in 0.15 M phosphate buffer (PB = 209-213 mosm/KgH2O, pH 7.39-7.41), colony cut-outs (CCO) were made with a scalpel.


Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4094
Author(s):  
Imran Ali ◽  
Nam Kyeun Kim ◽  
Debes Bhattacharyya

The integration of intumescent flame-retardant (IFR) additives in natural fiber-based polymer composites enhances the fire-retardant properties, but it generally has a detrimental effect on the mechanical properties, such as tensile and flexural strengths. In this work, the feasibility of graphene as a reinforcement additive and as an effective synergist for IFR-based flax-polypropylene (PP) composites was investigated. Noticeable improvements in tensile and flexural properties were achieved with the addition of graphene nanoplatelets (GNP) in the composites. Furthermore, better char-forming ability of GNP in combination with IFR was observed, suppressing HRR curves and thus, lowering the total heat release (THR). Thermogravimetric analysis (TGA) detected a reduction in the decomposition rate due to strong interfacial bonding between GNP and PP, whereas the maximum decomposition rate was observed to occur at a higher temperature. The saturation point for the IFR additive along with GNP has also been highlighted in this study. A safe and effective method of graphene encapsulation within PP using the fume-hood set-up was achieved. Finally, the effect of flame retardant on the flax–PP composite has been simulated using Fire Dynamics Simulator.


2017 ◽  
Vol 24 (1) ◽  
pp. 2-7 ◽  
Author(s):  
Kang Chen ◽  
Jinlong Yang ◽  
Hongbo Zhang ◽  
Wenjun Zhang
Keyword(s):  

2018 ◽  
Vol 4 ◽  
pp. 645-652 ◽  
Author(s):  
Laura L. Becerra ◽  
Juan A. Ferrua ◽  
Maxwell J. Drake ◽  
Dheekshita Kumar ◽  
Ariel S. Anders ◽  
...  
Keyword(s):  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Johnathan Kongoletos ◽  
Ethan Munden ◽  
Jennifer Ballew ◽  
Daniel J. Preston

AbstractVentilation, including fume hoods, consumes 40–70% of the total energy used by modern laboratories. Energy-conscious fume hood usage—for example, closing the sash when a hood is unused—can significantly reduce energy expenditures due to ventilation. Prior approaches to promote such behaviors among lab users have primarily relied on passive feedback methods. In this work, we developed a low-cost fume hood monitoring device with active feedback to alert lab users when a fume hood is left open and unused. Using data collected by the building management system, we observed a 75.6% decrease in the average sash height after installation of these “Motion and Sash Height” (MASH) alarms, which would result in a reduction roughly equal to 43% of the annual carbon emissions of a typical American vehicle, for each fume hood. The MASH alarm presented here reduced energy costs by approximately $1,159 per year, per hood, at MIT.


Sign in / Sign up

Export Citation Format

Share Document