scholarly journals Humoral serologic response to the BNT162b2 vaccine is abrogated in lymphoma patients within the first 12 months following treatment with anti-CD2O antibodies

Haematologica ◽  
2021 ◽  
Author(s):  
Ronit Gurion ◽  
Uri Rozovski ◽  
Gilad Itchaki ◽  
Anat Gafter-Gvili ◽  
Chiya Leibovitch ◽  
...  
Keyword(s):  
Positive Response ◽  
Vaccine Dose ◽  
Humoral Response ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples ◽  
Serologic Response ◽  
Antibody Titers ◽  
Anti Cd20

Patients with lymphoma, especially those treated with anti-CD20 monoclonal antibodies (MoAb), suffer high COVID-19-associated morbidity and mortality. The goal of this study was to assess the ability of lymphoma patients to generate a sufficient humoral response after two injections of BNT162b2 Pfizer vaccine and to identify factors impacting the response. Antibody titers were measured with the SARS-CoV-2 IgG II Quant (Abbott©) assay in blood samples drawn from lymphoma patients 4±2 weeks after the 2nd vaccine dose. The cutoff for a positive response was set at 50AU/ml. Positive serological responses were observed in 51% of the 162 patients enrolled in this cross-sectional study. In a multivariate analysis, an interval of

scholarly journals Comparison of capillary, venous and buffy coat blood samples in detecting Plasmodium species among malaria suspected patients attending at Hamusite health center. A cross-sectional study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Getu Abeje ◽  
Woyneshet Gelaye ◽  
Getaneh Alemu
Keyword(s):  
Health Center ◽  
Detection Rate ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Buffy Coat ◽  
Blood Film ◽  
Capillary Blood ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples

Abstract Background Both capillary and venous blood samples have been interchangeably used for the diagnosis of malaria in Ethiopia. However, Plasmodium parasites are thought to be more concentrated in capillary than in venous blood. Hence, selecting a sample source where parasites are more concentrated is indispensable approach in order to maximize the accuracy of blood film microscopy. Therefore, the present study aimed to compare the detection rate and the parasitemia level of Plasmodium species from conventional capillary and venous blood films, and buffy coat preparations. Methods A facility based cross-sectional study was conducted from Feburary to March 2020 among 210 febrile patients attending Hamusite health center, northwest Ethiopia. Capillary and venous blood samples were collected and buffy coat was prepared from each sample. Thin and thick blood films were prepared, stained, and examined microscopically following standard protocol. Data were analysed using Statistical Package for Social Sciences Software version 20 and Med-Calc software version 19.3. Results Capillary blood buffy coat (61/210, 29.0%) had significantly higher detection rate as compared to capillary (48/210, 22.9%) and venous (42/210, 20.0%) blood films (p < 0.001). However, no significant difference was observed between capillary and venous blood films (p = 0.070) in detecting Plasmodium species. The highest and the lowest mean asexual stage parasite counts were found in capillary blood buffy coat (4692.88) and venous blood (631.43) films, respectively showing significant variations (p < 0.001). Mean gametocyte count was also highest in capillary blood buffy coat (3958.44). As compared to capillary blood buffy coat, the sensitivity of venous blood buffy coat, capillary blood film and venous blood film were 73.8, 78.7, 68.9%, respectively. Conclusion Capillary blood buffy coat samples showed the highest sensitivity in detecting and quantitating malaria parasites that its use should be promoted in clinical settings. However, conventional capillary and venous blood films could be used interchangeably.

scholarly journals Hepatitis B antibody titers in Indonesian adolescents who received the primary hepatitis B vaccine during infancy

2013 ◽  
Vol 53 (3) ◽  
pp. 160
Author(s):  
Hartono Gunardi ◽  
Adra Firmansyah ◽  
Sri Rezeki S Harun ◽  
Sudigdo Sastroasmoro
Keyword(s):  
Hepatitis B ◽  
Booster Dose ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Tests ◽  
Vaccination History ◽  
Antibody Titers ◽  
Hb Vaccine ◽  
Over Time

Backgi-ound Hepatitis B (HB) has been classified as moderate-tohighlyendemic in Indonesia. HB vaccination, the most effectivemethod to prevent HB viral transmission, induces protectiveantibodies against HB surface antigen (anti-HBs). However, theseantibodies decline in titer over time. Studies on the duration ofprotection and the prevalence of n on-responders in Indonesianadolescents have been limited.Objectives To determine anti-HBs titers in 15-17-year oldIndonesian adolescents given primary HB vaccine during infancyand the prevalence of non-responders after a HB vaccine boosterdosage.Methods This cross-sectional study was performed from Februaryto September 2008 on adolescents aged 15-17 years in threesenior high schools in Jakarta who received complete primary HBvaccines during infancy, based on parents' recall. Investigationsincluded HB vaccination history, anthropometric measurements,and blood tests for anti-HBs before and 4-6 weeks after a boosterdose ofHB vaccine.Results Of 94 subjects, 35 had protective anti-HBs and 59 hadundetectable anti-HBs. A booster dose was administered to 5 8 of then on-protected subjects, of which 33 showed anamnestic responses.However, 25 subjects failed to generate protective anti-HBs. Takinginto consideration the adolescents with protective anti-HBs beforeand after the booster dose, serologic protection was demonstratedin 73%. Non-responder prevalence was 27%. The high prevalenceof non-responders may indicate bias of parents' recall.Conclusion Protective anti-HBs is detected in less than half ofIndonesian adolescents given primary HB vaccine during infancy.Following booster dosage, anamnestic responses are n oted in onethirdof subjects. The prevalence of non-responders is 27%, butconfirmation with further study is needed.

scholarly journals Dietary intake of vitamin B6 and concentration of vitamin B6 in blood samples of German vegans

2006 ◽  
Vol 9 (6) ◽  
pp. 779-784 ◽  
Author(s):  
A Waldmann ◽  
B Dörr ◽  
JW Koschizke ◽  
C Leitzmann ◽  
A Hahn
Keyword(s):  
Dietary Intake ◽  
Vitamin B6 ◽  
Cross Sectional Study ◽  
Dietary Vitamin ◽  
Vitamin B ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples ◽  
Daily Diet ◽  
High Bioavailability

AbstractObjectiveThe study aimed to evaluate the dietary vitamin B6 intake and determine the vitamin B6 concentration in blood samples of German vegans.Design and settingCross-sectional study with 33 examination sites all over Germany.SubjectsNinety-three vegans (50 females) with a mean (±standard deviation (SD)) age of 43.7 ± 15.7 years who took no vitamin supplements.MethodsDietary intake was assed using a semi-quantitative food-frequency questionnaire. Erythrocyte aspartate aminotransferase activity coefficient (EAST-AC) was calculated as the ratio of stimulated (pyridoxal 5'-phosphate added) to unstimulated activity in blood samples that were provided after an overnight fast.ResultsMean ± SD vitamin B6 intake was 2.83 ± 0.98 mg day−1 and mean ± SD protein intake was 56.6 ± 21.7 g day−1. Of the participants 4% showed vitamin B6 intakes lower than daily recommended intakes for Germany, 16% showed EAST-AC > 1.85, and a further 58% showed EAST-AC of 1.5–1-85. Moderate vegans were affected to a lesser extent than strict vegans. None of the established confounders was a significant predictor of EAST-AC. In logistic regression analyses the contribution of nutriments and cereals to pyridoxine intake was the only predictor of EAST-AC classified as < 1.85 and ≥ 1.85, respectively.ConclusionsIn spite of the high total intake of vitamin B6, an adequate concentration in blood samples could not be realised for a majority of the participants. Due to the health implications of a marginal pyridoxine status, vegans should be encouraged to include foods with a high bioavailability of pyridoxine, such as beans, lentils and bananas, in the daily diet.

scholarly journals Evaluating Relationships between Visceral Fat Measures and Adipokines Concentrations among Women with a History of Preeclampsia

2019 ◽  
Vol 37 (11) ◽  
pp. 1140-1145
Author(s):  
Suchitra Chandrasekaran ◽  
Darcy Barry ◽  
Susan Melhorn ◽  
Thomas Easterling ◽  
Hilary Gammill ◽  
...  
Keyword(s):  
Visceral Fat ◽  
Subcutaneous Fat ◽  
Secondary Analysis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Anthropometric Data ◽  
Cross Sectional ◽  
Blood Samples ◽  
Plasma Adiponectin ◽  
History Of

Abstract Objective We sought to evaluate associations between postpartum plasma adipokine concentrations among women with a history of preeclampsia (PE) without severe features (MPE), PE with severe features (SPE), and no PE (NPE). We also investigated relationships between adipokines and computed tomography (CT)-quantified measures of visceral fat (VF) area (VFA) and subcutaneous fat area (SCFA). Study Design We performed a secondary analysis of data collected as part of a previously performed cross-sectional study at our institution. Women with and without a history of PE were recruited in 10 years after delivery. VFA and SCFA measures were performed by CT scan. Anthropometric data and peripheral blood samples from subjects were collected concurrently. Results Plasma adiponectin concentrations (µg/mL) were significantly lower among MPE (18.5 ± 7.1) compared with NPE (27.3 ± 13.8) and SPE (25.7 ± 9.6). Leptin (p = 0.32) and resistin (p = 0.93) concentrations were similar among the groups. Adiponectin concentrations more closely aligned with VFA (β = −0.001, p = 0.03), while resistin concentrations trended toward correlating with SCFA (β = 0.02, p = 0.05). Leptin was not preferential to VFA or SCFA. Conclusion VF distribution may contribute to the variation in PE phenotype. Adiponectin specifically may be a promising marker representing VFA.

scholarly journals IgG, IgM, and IgA Antinuclear Antibodies in Discoid and Systemic Lupus Erythematosus Patients

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sheridan A. Jost ◽  
Lin-Chiang Tseng ◽  
Loderick A. Matthews ◽  
Rebecca Vasquez ◽  
Song Zhang ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Antinuclear Antibodies ◽  
Humoral Response ◽  
Cross Sectional Study ◽  
Discoid Lupus Erythematosus ◽  
Sectional Study ◽  
Systemic Lupus ◽  
Cross Sectional ◽  
Monkey Esophagus

IgG antinuclear antibodies (ANAs) are elevated in patients with systemic lupus erythematosus (SLE) compared with patients with discoid lupus erythematosus (DLE). To provide an expanded immunologic view of circulating ANAs in lupus patients, we compared the expressions of IgG, IgM, and IgA ANAs in DLE and SLE patients. In this cross-sectional study, sera from age-, gender-, and ethnic-matched SLEN=35, DLEN=23, and normal patientsN=22were tested for IgG, IgM, and IgA ANAs using enzyme-linked immunosorbent assays (ELISAs) and indirect immunofluorescence (IIF) with monkey esophagus as substrate. ELISAs showed elevated levels of IgG ANA, IgM ANA, and IgG/IgM ANA ratios in SLE patients compared with DLE and normal patients. IgA ANA expression was higher in SLE and DLE patients versus normal patients. IIF studies showed higher percentages of patients positive for IgG, IgM, and IgA ANAs in the SLE group. Higher IgG/IgM ANA ratios in SLE than DLE show enhanced class-switching and a more sustained humoral response in SLE. They also suggest a potential connection of IgM ANAs with disease containment.

scholarly journals MILK AGGRAVATES ASTHMA – THE TRUTH OR THE PERCEPTION

2020 ◽  
Vol 70 (6) ◽  
pp. 1691-95
Author(s):  
Muhammad Khalid Azam Khan ◽  
Shazia Naz ◽  
Abdul Latif Khattak ◽  
Nauman Kashif ◽  
Syed Karamat Hussain Shah Bukhari ◽  
...  
Keyword(s):  
Positive Response ◽  
Cross Sectional Study ◽  
Military Hospital ◽  
P Value ◽  
Sectional Study ◽  
Cross Sectional ◽  
Medicine Department ◽  
Milk Substitute ◽  
Dyspnea Score ◽  
Drinking Milk

Objective: To assess the notion that milk can cause or aggravate asthma and break this myth. Study Design: A cross sectional study. Place and Duration of Study: Medicine department, Combined Military Hospital Lahore, from Jan 2019 to Dec2020. Methodology: A survey was conducted whereby known asthmatics were questioned about their opinion weather milk aggravates their asthma. Those who confirmed their positive response were exposed to milk and some liquid resembling milk, and their Lung functions were tested after each drink. The 42 patients who considered milk as the primary aggravator were called for the study for spirometry Results: A total of 600 people from the general population were surveyed. Two hundred and six (34.3%) of them were illiterate, 394 (65.6%) were educated from middle to graduates. In the first milk/milk encounter, the combined dyspnea scale showed increase of 50.3% and improvement of 0.008% in FEVI/FVC. In the second substitute/milk encounter, the dyspnea score showed an increase of 61.8% and an increase of 0.90% in FEVI/FVC. In the third milk/substitute encounter the dyspnea score showed a decrease of 22.9% and the FEVI/FVC decreases by 5.5%. In the 4th substitute/substitute encounter, dyspnea scores decreased by 8.4% while the pre and postencounter FEVI/ FVC scores were 2726 and 2711 with 0.55% decrease with insignificant p-value >0.05. Conclusion: Drinking milk or milk substitute has negligible effect on the spirometric parameters.

scholarly journals Tetanus vaccine coverage in recommended and more than recommended doses among mothers in a West Cameroon health district: a cross sectional study

2021 ◽  
Vol 4 ◽  
pp. 46
Author(s):  
Igor Nguemouo Nguegang ◽  
Martin Nguetsop ◽  
Linda Evans Eba Ze ◽  
Trevor Anyambod Mboh ◽  
Dominique Majoric Omokolo ◽  
...  
Keyword(s):  
Vaccine Coverage ◽  
Vaccine Dose ◽  
Immunization Coverage ◽  
Cross Sectional Study ◽  
Health District ◽  
Cluster Sampling ◽  
Cumulative Number ◽  
Sectional Study ◽  
Cross Sectional ◽  
Tetanus Vaccine

Background: Vaccination is the best way to protect newborns and mothers against tetanus. The number of doses of Td vaccine recommended by the Expanded Program of Immunization is based on documented protective immune response. During this decade, the Foumban Health District (FHD) was the seat of an epidemic despite the satisfactory administrative immunization coverage. This study was conducted in 2019 in the FHD to assess tetanus vaccine coverage among mothers for their last pregnancy and the cumulative number of vaccine doses administered to the mothers. Method: This was a descriptive cross-sectional study conducted in the FHD in 2019. Mothers living in the district with at least one child younger than five years were included and were selected by random stratified cluster sampling. Trained surveyors used a face-to-face questionnaire, data extraction grid and data-tracking grid to review and collect data from antenatal care booklets, vaccination cards and the women’s own reports of immunization. The immunization coverage per vaccine dose and vaccination completeness rate were estimated. Results: From 621 women visited, 602 (96.9%) responded. A total of 176/511 women (34.4%) had evidence of vaccination. For the last pregnancy, the two-dose immunization proportion was 21.7% (111/511) for documented coverage and 47.6% (243/511) for undocumented coverage. 306/570 women (53.7%) had received more than the recommended five doses necessary for lifetime protection. The recruitment, two and five doses completeness rates were 99.9% (569/570), 95.8% (546/570) and 65.3% (372/570), respectively. Conclusion: More than half of mothers received more doses of tetanus toxoid vaccine than necessary in FHD. This increased the resources needed and the cost of vaccination. Health personnel should be trained and supervised to assess pregnant women’s vaccination status before planning the number of vaccine doses to be administered during pregnancy.

scholarly journals Long-Term Duration of Antibody Response to SARS CoV-2 in One of the Largest Slums of Buenos Aires

2021 ◽  
Author(s):  
Vanina Pagotto ◽  
Lorena Luna ◽  
Julieta Salto ◽  
Magdalena Wagner Manslau ◽  
Silvana Figar ◽  
...  
Keyword(s):  
Antibody Response ◽  
Buenos Aires ◽  
Humoral Response ◽  
Cross Sectional Study ◽  
Older Individuals ◽  
Sectional Study ◽  
Cross Sectional ◽  
The People ◽  
Igg Level

AbstractThe durability of the antibody response following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections has not been fully elucidated. We have performed a cross-sectional study in one of the largest slums of Buenos Aires, Barrio Padre Mugica in June 2020, detecting a seroprevalence of 53.4%. To evaluate the persistence of the humoral response against SARS-CoV-2 in this area, we designed a second study assessing only the people that were IgG positive in the first survey. The IgG levels against the full spike (S) protein in 175 individuals that were seropositive, at least 6 months before, were evaluated in a second survey. The positivity rate was 92.0%, 161 from 175 individuals remained IgG positive. We observed a contraction in the overall IgG levels measured by ELISA. The median IgG dropped 62% from June to December 2020. Most of the individuals tested (87%) reported to be asymptomatic or oligo-symptomatic. No difference was found between men and women, but people aged less than 50 showed a lower IgG level in each period compared to older individuals. Our data indicate sustained humoral immunity for at least 6 months in a specific socio-economical setting in a population that was mainly asymptomatic for COVID-19.

scholarly journals COMPARISON OF AUTOMATIC STARRSED RS ERYTHROCYTE SEDIMENTATION RATE WITH MANUAL WESTERGREN METHOD

2019 ◽  
Vol 25 (3) ◽  
pp. 290
Author(s):  
Ninik Sukartini ◽  
Siskawati Suparmin
Keyword(s):  
Sedimentation Rate ◽  
Cross Sectional Study ◽  
Systematic Difference ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
Using Data ◽  
Good Agreement

This study aimed to compare the results of ESR measurements using Starrsed RS (30- and 60-minute mode) and manual Westergren method. We also investigate the effect of high leukocytes and haematocrit (Ht) on Starrsed RS ESR measurements. A cross-sectional study using K3EDTA anticoagulated blood samples from 140 subjects were conducted. A total of 21 samples with leukocytes count >50 000/µl were examined. The effect of Ht was studied using data from comparative study subjects.  The 60-minute mode Starrsed RS correlated very strongly [r = 0.98 (95%CI 0.97 to 0.98), p <0.0001], had good agreement [mean bias -0.4 mm (95%CI -1.46 to 0.74)], and gave no significant different results with manual Westergren method. The 30-minute mode Starrsed RS also has a very strong correlation [r = 0.97 (95%CI 0.96 to 0.98), p<0.0001] and good agreement [mean bias-2.9 mm (95%CI -4.23 to -1.58)], but has systematic difference with manual Westergren method. There was significant difference between manual method and Starrsed RS in samples with high leukocytes (p = 0.0208). There were no significant differences of ESR results measured by Starrsed RS dan manual Westergren method in the group of Ht≤35% and >35%. The ESR on the Starrsed RS should still be read at 60 minute. Leukocytes>50 000/µl may affect ESR measurements on Starrsed but no effect of haematocrit was observed.

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