Association of Hyperhomocysteinemia with Early Onset Atherosclerotic Peripheral Arterial Occlusive Diseases

2021 ◽  
Vol 13 (2) ◽  
pp. 120-127
Author(s):  
Md Shamim Reza ◽  
AM Asif Rahim ◽  
AHM Bashar ◽  
M Shahidul Islam ◽  
SAN Alam
Keyword(s):  
Early Onset ◽  
Homocysteine Level ◽  
Histopathological Examination ◽  
Serum Homocysteine ◽  
Group I ◽  
Arterial Occlusive Diseases ◽  
Total Homocysteine ◽  
Group Ii ◽  
Peripheral Arterial ◽  
Plasma Total Homocysteine

Background: Homocysteine is increasingly recognized as an independent risk factor for vascular disease specially peripheral arterial occlusive diseases. In the present study, an attempt was made to find out the association between the raised fasting plasma total homocysteine level and early onset atherosclerotic peripheral arterial occlusive diseases (PAOD). Methods: In this prospective observational study a total of 50 study subjects were included. All patients were clinically and angiographically documented for atherosclerotic PAOD. All patients underwent surgical intervention as well as estimation of serum total homocysteine level. Arterial segment was sent for histopathological examination to find out whether atherosclerosis was present or not. Patients were divided into two groups- Group-1 included patients of 20-40 years of age and group-II included those of 41-60 years of age. The groups were compared to see association between elevated level of plasma homocysteine and atherosclerotic peripheral arterial occlusive diseases in elderly as well as in early age. Results: 41 (82%) patients were male and 9 (18%) patients were female. Serum homocysteine level was higher in group I than group II (71.4% vs. 40.9%). Besides, the level of mean serum homocysteine level was significantly (p=0.02) higher in group I than group II (21.18±9.53 vs. 17.24±8.92 μmol/L). Conclusion: In conclusion, this study suggests that serum homocysteine has an association with early onset atherosclerotic PAOD. Therefore, a raised serum homocysteine level can be used as an independent biochemical predictor of early onset atherosclerotic PAOD. Cardiovasc. j. 2021; 13(2): 120-127

scholarly journals Alleviation of Plasma Homocysteine Level by Phytoestrogenα-Zearalanol Might Be Related to the Reduction of Cystathionineβ-Synthase Nitration

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Hui Zhang ◽  
Qi Sun ◽  
Teng Liu ◽  
Lu Ma ◽  
Panpan Zhen ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Homocysteine Level ◽  
Plasma Homocysteine ◽  
Plasma Homocysteine Level ◽  
Rat Models ◽  
Nitrative Stress ◽  
Key Enzyme ◽  
Total Homocysteine ◽  
Underlying Mechanisms ◽  
Plasma Total Homocysteine

Hyperhomocysteinemia is strongly associated with cardiovascular diseases. Previous studies have shown that phytoestrogenα-zearalanol can protect cardiovascular system from hyperhomocysteinemia and ameliorate the level of plasma total homocysteine; however, the underlying mechanisms remain to be clarified. The aim of this research is to investigate the possible molecular mechanisms involved in ameliorating the level of plasma homocysteine byα-zearalanol. By the successfully established diet-induced hyperhomocysteinemia rat models, we found that, afterα-zearalanol treatment, the activity of cystathionineβ-synthase, the key enzyme in homocysteine metabolism, was significantly elevated and level of nitrative stress in liver was significantly reduced. In correlation with this, results also showed a decreased nitration level of cystathionineβ-synthase in liver. Together data implied that alleviation of plasma homocysteine level by phytoestrogenα-zearalanol might be related to the reduction of cystathionineβ-synthase nitration.

scholarly journals Preoperative management in Hirschsprung’s congenital megacolon

2015 ◽  
Vol 10 (3) ◽  
pp. 276-280
Author(s):  
Simona-Gabriela TUDORACHE ◽  
◽  
Cătălin CHIRIAC-BABEI ◽  
Toli PĂTRĂNCUŞ ◽  
Laura NICULESCU ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Average Length ◽  
Treatment Plan ◽  
Congenital Megacolon ◽  
Group I ◽  
Definitive Operation ◽  
Group Ii ◽  
Pull Through ◽  
Definitive Surgery ◽  
Time Of Operation

Introduction. Hirschsprung’s congenital megacolon is a challenge in terms of diagnosis and a proper treatment plan. If, in terms of the final operation there have been described various techniques and done studies that followed postoperative complications, regarding tracking and treatment previous to the intervention there is little documentation available. Material and methods. The study is retrospective and includes a group of 31 patients diagnosed and treated in the “Grigore Alexandrescu” Children’s Emergency Hospital between 2010-2014. The group of patients was divided into two groups: Group I – patients who underwent diverting stomy before pull through and group II – without diverting stomy, who received preoperative nursing. The main parameters followed were: the frequency rate of enterocolitis and number of hospitalizations before the definitive operation. The second parameter followed is the patients age at the time of the pull through, patients were followed until definitive surgery. Data was collected from the hospital information system – Hippocrates, the clinical charts and the operation room registry of surgery and were processed using SPSS version 22.0. Results. From group I, 10 patients (32.3%) had at least one episode of enterocolitis before definitive surgery, compared with only 3 patients (9.7%) in group II. Analyzing the data, we fi nd that patients in group I have on average 4 more hospitalizations than those in group II. The average length hospitalization was 21 days for group I and 7 days for group II. The age at the time of operation ranged between 2 and 72 months in the whole group, with an average of 8 months for patients in group II and 21 months in the first group. The average duration of observation of the patients was 3 months for group I and 16 months for the second group. Conclusion. In the group with diverting stomy, infectious episodes are more frequent and the number of hospitalizations before the pull through is bigger. If frozen histopathological examination is available one stage pull through is considered the best option, exception beeing when there is an absolute indication for diverting stomy.

scholarly journals The Effect of Intratesticular Injection of Human Adipose-Derived Stem Cell (hADSC) on Testicular Oxidative Stress and Spermatogenesis Process in the Varicocele Rat Model

2021 ◽  
Author(s):  
Safendra Siregar ◽  
Bambang Sasongko Noegroho ◽  
Ricky Adriansjah ◽  
Akhmad Mustafa ◽  
Ananta Bonar
Keyword(s):  
Oxidative Stress ◽  
Rat Model ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Therapy Group ◽  
Intervention Group ◽  
Testicular Tissue ◽  
Control Group ◽  
Group I ◽  
Group Ii

Abstract Introduction: Varicocele is the predominant cause of male infertility and was found in 19% - 41% of men with primary infertility and 45% - 81% of men with secondary infertility. Human adipose Derived Stem Cells (hADSC) can suppress oxidative stress in some oxidative injury model. Therefore, this study would like to investigate the effect of intratesticular hADSC injection on MDA level and spermatogenesis process by histopathological examination in the varicocele rat model.Method: This is an experimental study. A total sampling of 9 male Wistar rats were divided into three groups. Group I consist of 1 Wistar rats without any treatment or model (sham group), group II consist of 4 Wistar rats with varicocele model without hADSC therapy (control group), and group III consist of 4 Wistar rats with varicocele model and were given injections of 1.0x106 hADSC cells intratesticularly 30 days after model was made (therapy group). Testicular tissue was harvested for evaluation. Results: In all varicocele model rats (group II and III), the result of MDA level in therapy group (2.53 mol/liter) was significantly lower than the MDA level in control group (4.43 mol/liter) (p = 0.01). On histopathological examination, the average Johnson's Score in the therapy and control group was 9,77 and 9,18, respectively. The analysis showed Johnson’s score in the intervention group was significantly higher (p = 0.018). Conclusion: Intratesticular injection of hADSC can help reduce MDA levels and improve spermatogenesis process, which is damaged by varicoceles.

scholarly journals Effectiveness of capsaicin containing dried habanero pepper extract in the treatment of primary hepatic cancer in geriatric dogs

2018 ◽  
Vol 74 (1) ◽  
pp. 5976-2018
Author(s):  
PIOTR DĘBIAK ◽  
DAGMARA GADOMSKA* ◽  
ANNA ŚMIECH ◽  
JERZY ZIĘTEK ◽  
PAWEŁ ŁYP ◽  
...  
Keyword(s):  
Dietary Supplement ◽  
Histopathological Examination ◽  
Hepatic Cancer ◽  
Liver Tumours ◽  
Group I ◽  
Average Survival ◽  
Habanero Pepper ◽  
Long Time ◽  
Group Ii ◽  
Alternative Medications

The study was aimed at the preliminary clinical assessment of the anti-cancer efficacy of a dietary supplement based on the extract of habanero peppers containing capsaicin in dogs with primary hepatic cancer. 24 dogs with primary hepatic cancer, divided into two groups, were followed up during the study. Group I included 16 dogs receiving a dietary supplement based on capsaicin-containing habanero peppers, while group II consisted of 8 dogs that did not receive any veterinary treatment. The dogs were subjected to clinical examination, ultrasound imaging as well as biochemical and haematological analyses. Patient survival was determined in both groups. An autopsy was performed in all animals that died or were euthanised, and tumour samples were collected for histopathological examination, on the basis of which the type of neoplasm was finally determined. Survival of dogs in group I ranged from 54 to 285 days (178.6 days on average). Survival of dogs in group II was 32-128 days (76.5 days on average). In none of the dogs classified to group I that had single tumours did the size of the lesion increase during supplement administration, and in the case of two dogs that were receiving the preparation for a long time (188 and 285 days), their liver tumours actually shrank. In all dogs from group II that had single tumours, the size of the lesions increased in the course of the observation. The preliminary clinical observations by the authors indicate that the capsaicin-containing habanero pepper extract exhibits favourable effects on liver tumours in dogs and is well tolerated by the animals, thus the obtained results are a good sign for future studies on alternative medications used in dog oncology..

scholarly journals Biochemical and Histopathological Effects on Liver Profile due to Acute and Subacute Oral Toxicity of Somina on Rats

2021 ◽  
Vol 9 (1) ◽  
pp. 76-81
Author(s):  
Aisha Azmat ◽  
Muhammad Ahmed
Keyword(s):  
Oral Administration ◽  
Histopathological Examination ◽  
Treated Group ◽  
Control Group ◽  
Histopathological Analysis ◽  
Oral Toxicity ◽  
Toxicological Effect ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Background: Limited research studies are reported regarding the toxicological effect of different herbal medicine already used in different countries. Objective: This research study was planned to examine the changes in liver (biochemical and histological) associated with oral administration of somina (acute and sub-acute) in rats. Methodology: Group– I served as control (saline), while other groups (II, III) were daily treated with somina at different doses of 0.285g/kg (group – II), 10g/kg/day (group – III), for 14 (set I), 21 (set II), and 30 (set III) consecutive days.  Each group contains 12 rats. During the study period, signs and behavioral changes, mortality, were observed. At the end of study period, blood sample was drawn directly from heart, for the estimation of liver enzymes: Bilirubin (BIL), alkaline phosphatase (ALP), serum glutamic pyruvic transferase (SGPT), aspartate aminotransferase (SGOT), Albumin (ALB) and total protein (TP). The liver was carefully dichotomized, weighed, and further processed for histopathological analysis. Results: Herbal drug somina was claimed to be practically non-toxic as in rats no mortality was recorded after the oral administration of somina (14, 21 and 30 consecutive days). Liver profile showed non-significant changes in treated group- II and III (P > 0.05), as compared to the control (group- I). The histopathological examination did not reveal any deteriorative effect. Conclusion: It was concluded that oral administration of somina did not produce any significant detrimental effects on rat liver (biochemical and histopathological parameters), even at doses of 10g/kg/day indicating its safe use.

Relationship between plasma total homocysteine level and dietary caffeine and vitamin B6intakes in pregnant women

2013 ◽  
Vol 16 (2) ◽  
pp. 164-170 ◽  
Author(s):  
Mie Shiraishi ◽  
Megumi Haruna ◽  
Masayo Matsuzaki ◽  
Erika Ota ◽  
Ryoko Murayama ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Homocysteine Level ◽  
Total Homocysteine ◽  
Plasma Total Homocysteine
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