scholarly journals Rheumatoid Factor, Anti-cyclic Citrullinated Peptide and HLA-B Locus Antigens in Psoriatic Arthritis Patients in Bangladesh

2022 ◽  
Vol 12 (2) ◽  
pp. 109-114
Author(s):  
Devolina Bhowmik ◽  
Manash Chandra Sarker ◽  
Shirin Tarafder ◽  
Hosne Jahan ◽  
Mst Naznin Tarana ◽  
...  
Keyword(s):  
Psoriatic Arthritis ◽  
Rheumatoid Factor ◽  
Human Leukocyte ◽  
Cross Sectional Study ◽  
P Value ◽  
Serum Samples ◽  
Cross Sectional ◽  
Cyclic Citrullinated Peptide ◽  
B Locus ◽  
Citrullinated Peptide

Introduction: Psoriatic arthritis (PsA) is established as a multifactorial disease resulting from a complex interplay between genetic, environmental and immunological factors. It is a seronegative arthritis but rheumatoid factor may be present in up to 15% of PsA patients Antibodies recognizing a cyclic citrullinated peptide are highly speciûc for rheumatoid arthritis (RA) but their role in PsA remains unclear. An increased prevalence of anti-CCP antibody in PsA is also reported. Study shows that HLA-DRB1 shared epitope is signiûcantly associated with the presence of anti-CCP antibody in PsA patients but this type of association is not found with other human leukocyte antigens. Objectives: The aim of this study was to investigate the frequency of anti-CCP and RF in PsA patients and their associations with HLA-B locus antigens. Methods: In this cross sectional study, we selected 50 unrelated consecutive patients with PsA according to CASPAR criteria for PsA. 6 ml of blood was collected from each patient for HLAB locus typing, RA test and test for anti-CCP. Patient’s serum samples were tested for RF by Nephelometric system and tests for anti-CCP were done by ELISA. HLA-B locus typing was done by PCR with sequence specific primer. Results: Among 50 PsA patients, 27 (54%) are female and 23 (46%) are male. RA test is positive in 10 (20%) patients and anti-CCP is positive in 7 (14%) patients. Significant association was found between HLA-B*37 and RF (p value= < 0.001). Conclusion: RF is present in 10 (20%) and anti-CCP is present in 7 (14%) PsA patients. HLAB* 37 was significantly found in RF positive patients. J Shaheed Suhrawardy Med Coll 2020; 12(2): 109-114

scholarly journals ANTI-CYCLIC CITRULLINATED PEPTIDE (ANTI-CCP) ANTIBODIES IN PSORIATIC ARTHRITIS: A CROSS-SECTIONAL STUDY IN BRAZILIAN PATIENTS

2019 ◽  
Author(s):  
CRISTIANE GRUBER ◽  
THELMA LAROCCA SKARE ◽  
ANA PAULA BECKHAUSER DE CAMPOS ◽  
VINÍCIUS MAESTRI ◽  
BETANIA LONGO ◽  
...  
Keyword(s):  
Psoriatic Arthritis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Cyclic Citrullinated Peptide ◽  
Citrullinated Peptide

scholarly journals Relationship Between Rheumatoid Factor Isotypes and IgG Anti-Cyclic Citrullinated Peptide Antibodies

2010 ◽  
Vol 37 (8) ◽  
pp. 1582-1588 ◽  
Author(s):  
TROY D. JASKOWSKI ◽  
HARRY R. HILL ◽  
KATHERINE L. RUSSO ◽  
GABRIELLA LAKOS ◽  
ZOLTAN SZEKANECZ ◽  
...  
Keyword(s):  
Rheumatoid Factor ◽  
Lupus Erythematosus ◽  
Blood Donors ◽  
Clinical Value ◽  
Serum Samples ◽  
Cyclic Citrullinated Peptide ◽  
Rheumatoid Factor Isotypes ◽  
To Come ◽  
Testing Algorithm ◽  
Citrullinated Peptide

Objective.To validate in a general patient population (GPP) the clinical value of measuring rheumatoid factor (RF) isotypes in relationship to IgG anti-cyclic citrullinated peptide (CCP) antibodies (CCP2 and CCP3).Methods.Serum samples were obtained as follows: 1021 GPP, for whom RF was ordered for diagnosis, 137 with rheumatoid arthritis (RA), 100 healthy blood donors (HBD), and 50 with systemic lupus erythematosus. Turbidimetry and ELISA were utilized for RF screening, and individual RF isotypes and IgG anti-CCP antibodies were measured by ELISA; RF IgG was measured after pepsin digestion.Results.We validated the generally accepted 90%–98% positive predictive value (PPV) and about 68% sensitivity of the anti-CCP2 test on our diagnosed cohorts as 96% (95% CI 89–99) and 65% (95% CI 56–73), respectively. The 282 RF IgM+ specimens identified in the GPP were subdivided into 3 subsets: (1) 83 as RF IgM+ IgG+ IgA+ with 63% (95% CI 51–73) anti-CCP2+ (i.e., sensitivity similar to the RA cohort); (2) 50 as RF IgM+ IgG− IgA+ with significantly fewer anti-CCP2+ (22%; 95% CI 12–36); and (3) about half as IgM+ IgG− IgA− with just 3% (95% CI 1–8) anti-CCP2+, i.e., not significantly different from the 1% (95% CI 0–5) in HBD. Thus, the chance for a specimen in the GPP to be anti-CCP2+ (i.e., to come from an RA patient) was increased by 7- and 21-fold, respectively, by identifying RF IgA and IgG in addition to IgM. About one-third of anti-CCP− RA patients in our cohort were RF IgM+ IgG+ IgA+, reflected as 3.4% in the anti-CCP2− GPP. The agreement between anti-CCP2 and anti-CCP3 was significantly higher for RF+ RA and GPP patients, 86% (95% CI 78–93) and 83% (95% CI 73–91), respectively, than for the RF− RA (27%; 95% CI 6–61), RF− GPP (4%; 95% CI 0–19), and non-RA controls. Anti-CCP2 but not anti-CCP3 significantly distinguished the HBD from the GPP (95% CI).Conclusion.Measurement of the 3 isotypes of RF may increase by 7- to 21-fold the chance of making the serologic diagnosis of RA; a testing algorithm is proposed. The anti-CCP antibody response appears significantly less peptide-specific in the presence of IgM RF than in its absence.

scholarly journals Circulation of Toscana Virus in a Sample Population of Corsica, France

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 39
Author(s):  
Shirley Masse ◽  
Nazli Ayhan ◽  
Lisandru Capai ◽  
Rémi Charrel ◽  
Alessandra Falchi
Keyword(s):  
Blood Donors ◽  
Cross Sectional Study ◽  
P Value ◽  
Healthy Population ◽  
Sample Population ◽  
Surveillance Systems ◽  
Toscana Virus ◽  
Serum Samples ◽  
Cross Sectional ◽  
Study Participants

Sandfly-borne phleboviruses pathogenic to humans, such as Toscana virus (TOSV) and sandfly fever Sicilian virus (SFSV), are endemic in the Mediterranean region. In France, several autochthonous cases of TOSV infection have been described which cause either meningitis or encephalitis. The aim of the present study was to estimate the seroprevalence of TOSV and SFSV antibodies in a healthy population from Corsica. In this cross-sectional study, participants were enrolled from the medical staff at University of Corsica Pasquale Paoli (UCPP) and from general practitioners of the Corsican Sentinelles Network. The seroprevalence study was based on virus microneutralization (MN). A total of 240 patients were tested for TOSV and SFSV. Altogether, 54 serum samples were confirmed for TOSV infection (seroprevalence = 22.5%). None of the samples were positive for SFSV (0/240). The main place of residence was significantly associated with TOSV seropositivity (p-value = 0.005). The overall rate of TOSV antibody seroprevalence observed in our study suggests a more intense circulation of TOSV in Corsica, with a rate significantly higher than the 8.7% reported in Corsica in 2007 from blood donors. The absence of seropositivity to SFSV seems to confirm the low circulation of this virus in Corsica and in continental France. The increasing circulation of TOSV reported here should encourage the implementation of surveillance systems to control phlebovirus infection.

scholarly journals Prevalence and risk factors profile of seropositiveToxoplasmosis gondiiinfection among apparently immunocompetent Sudanese women

2019 ◽  
Author(s):  
Madinna Mustafa ◽  
Fatima Fathy ◽  
Abubaker Mirghani ◽  
Mona A. Mohamed ◽  
Mohamed S. Muneer ◽  
...  
Keyword(s):  
Risk Factors ◽  
Meat Consumption ◽  
Cross Sectional Study ◽  
Married Women ◽  
P Value ◽  
Serum Samples ◽  
Cross Sectional ◽  
Raw Meat ◽  
Factors Analysis ◽  
Potential Risk Factors

AbstractObjectivesToxoplasma gondiiis an opportunistic parasite that cause a clinical manifestation known as toxoplasmosis. We investigated the prevalence and potential risk factors ofT. gondiiinfection among women in Khartoum, Sudan. A sero-parasitological cross-sectional study included 100 women aging between 15-50 years old was conducted in Khartoum, Sudan between January – November 2018. Serum samples were collected and investigated for presence of anti-T. gondiiimmunoglobulins.ResultsMean age of the women population included was 26.75±8.25 with a range between 15 and 50 years. Sero-prevalence ofT. gondiiantibodies was 27% (27/100) with a 95% confidence interval (CI) of 18.6 – 36.8%. Among seropositive population 81% (22/27), 15% (4/27) and 4% (1/27) were seropositive for IgG antibodies, IgM antibodies and both antibodies respectively. Age group 21-30 years old had the highest frequency of detected IgG (10/45) and IgM (3/45). Married women had the highest frequency of detected IgG or IgM, 18/79 and 3/79, respectively. Risk factors analysis showed a total of 37/100 participants were having direct contact with cats and 66/100 have a frequent raw meat consumption, neither direct cats contact nor raw meat consumption had statistically significant association with seropositivity to T. gondii (P. value =0.052 and 0.565, respectively).

scholarly journals The Diagnostic Value of Anti-Cyclic Citrullinated Peptide Antibodies (anti-CCP) in Patients with Rheumatoid Arthritis

2007 ◽  
Vol 26 (4) ◽  
pp. 285-288
Author(s):  
Ljubinka Damjanovska ◽  
Violeta Atanasovska ◽  
Todor Gruev
Keyword(s):  
Rheumatoid Arthritis ◽  
Diagnostic Value ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Mean Values ◽  
Cyclic Citrullinated Peptide ◽  
The Mean ◽  
Citrullinated Peptide ◽  
Peptide Antibodies ◽  
Latex Test

The Diagnostic Value of Anti-Cyclic Citrullinated Peptide Antibodies (anti-CCP) in Patients with Rheumatoid ArthritisA cross-sectional study was carried out to analyze the prevalence of anti-CCP antibodies and IgM RF in the sera of 160 randomly selected patients from the Rheumatology Department: 60 with RA, 50 with other rheumatic diseases (non-RA), 50 healthy controls (HC). The mean age of the group was 50.06+/-11.9 years. There were 141 females (88.1%) and 19 males (11.9%). RA patients fulfilled the revised ACR criteria. The mean duration of the disease was 82.4 months. Anti-CCP ELISA kit and IgM RF Latex test were used. The mean anti-CCP values were as follows: RA 60.4+/-57.6, non-RA 2.1+/-3.6, HC 1.3 +/-0.4 U/mL. Respectively, the mean values of IgM RF were: RA 515.8+/-525, non-RA 102+/-294, HC 15+/-57.5. Forty out of 60 (66.6%) RA patients were anti-CCP positive. Forty one out of 60 (68.3%) RA patients were positive for IgM RF. As expected, anti-CCP showed comparable sensitivity (66.8% vs. 68.3%) and higher specificity (98% vs. 87%) than IgM RF, at optimal cut-off values. The presence of either anti-CCP or IgM RF increased the testing sensitivity for the diagnosis of RA to 76.6%. AUC was greater for anti-CCP than for IgM RF (0.92 vs. 0.82).

USE OF ANTI-CYCLIC CITRULLINATED PEPTIDE (ANTI-CCP) ANTIBODIES AND RHEUMATOID FACTOR (RF) IN DIAGNOSIS OF RHEUMATOID ARTHRITIS

2021 ◽  
pp. 67-70
Author(s):  
Rahul Ranjan ◽  
Md Shoeb Alam ◽  
V N Jha
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Treatment Decision ◽  
Pulmonary Involvement ◽  
Diagnostic Value ◽  
Cross Sectional Study ◽  
P Value ◽  
Cross Sectional ◽  
Medical College ◽  
Combined Use

INTRODUCTION: Rheumatoid arthritis (RA) is a chronic, systemic, inammatory autoimmune disease that initially affects small joints, progressing to larger joints, and eventually the skin, eyes, heart, kidneys, and lungs. This study was conducted to determine the usefulness of antiCCP antibodies and Rheumatoid Factor (RF) in diagnosis of Rheumatoid Arthritis and to estimate the diagnostic value of anti-CCP antibodies compared with Rheumatoid Factor (RF) in the diagnosis of Rheumatoid Arthritis. MATERIALS AND METHODS: This study was conducted in the Department of Medicine and it was a cross sectional study. A total of 86 patients presented with clinically suspected rheumatoid arthritis who attended the OPD Medicine were consecutively recruited. The present study was conducted on patients with clinically suspected rheumatoid arthritis from Department of Medicine, Darbhanga Medical College & Hospital, Laheriasarai, Bihar over a period of 12 months. RESULT: Anti-CCP antibody was not signicantly associated with the disease activity (p value =0.36). Anti-CCP antibody was signicantly associated with radiological defects whereby majority of patients with radiological defects (n=21/30; 70%) were positive for anti-CCPantibody (p value=0.03). Anti-CCP antibody was not signicantly associated with the incidence of rheumatoid nodule (p value =0.750). Anti-CCP antibody was not signicantly associated with extra-articular manifestation (p value =0.398). There is no signicant association between anti-CCPantibody and incidence of pulmonary involvement (p value =0.367). CONCLUSION: Combined use of RF and anti-CCP is a better prognostic and diagnostic tool than conventional RF tests alone. Uses of anti-CCP in clinical practice contribute to enhance the ability of rheumatologists to make judicious treatment decision. The usage of anti-CCP antibody is useful in the detection of early disease as evidenced by signicant association between anti-CCPantibody, RF, and radiological involvement in our study.

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