scholarly journals Antibiotic resistance profiling of clinical isolates of Salmonella enterica Serovar Paratyphi A in Dhaka, Bangladesh

2021 ◽  
Vol 11 (1) ◽  
pp. 14-16
Author(s):  
Md Mobarok Hossain ◽  
Tasnim Jabin ◽  
Md Ifrat Hossain ◽  
Mst Arzina Khatun ◽  
Md Hossain Emam ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Health Management ◽  
Age Groups ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Antibiotic Resistance Profile ◽  
Conventional Culture ◽  
Drug Resistance Pattern

The study was conducted to detect the antibiotic resistance profile of the clinical isolates of Salmonella enterica Serovar Paratyphi A from 100 blood samples of patients from different age groups suspected to be suffering from enteric fever. The pure cultures of the bacterial isolates were collected from some renowned diagnostic centers of Dhaka and they were further characterized through the conventional culture, microscopy and biochemical examinations. These isolates were cross checked for the antibiogram profile by the Kirby-Bauer disc diffusion method against ten different types of antibiotics. Most of the isolates were found resistant against azithromycin (100%), nalidixic acid (100%) and ceftazidime (75%). However, isolates showed sensitivity to ciprofloxacin (95%), levofloxacin (97%), cotrimoxazole (96%) and chloramphenicol (95%). These findings highlight the need for continuous monitoring of the drug resistance pattern of S. enterica Serovar Paratyphi A for better public health management. Stamford Journal of Microbiology, Vol.11 (1) 2021: 14-16

Distribution of Pathogens Involved in Infections in Oncological Patients and their Antibiotic Resistance Profile

2020 ◽  
Vol 70 (12) ◽  
pp. 4287-4294
Keyword(s):  
Antibiotic Resistance ◽  
Cancer Patients ◽  
Bacterial Pathogen ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Antibiotic Resistance Profile ◽  
Oncological Patients ◽  
Eastern European Countries ◽  
Klebsiella Spp

Cancer is the second leading cause of death in Romania and worldwide. Cancer patients are at increasing risk of acquiring bacterial infection with multi-resistant germs, including multidrug-resistant (MDR) strains of Gram-negative bacteria involved in nosocomial infection. Romania is one of the South-Eastern European countries with one of the highest prevalence rates of MDR pathogens. To determine the resistance pattern of bacterial profile and antibiotic resistance pattern in cancer patients admitted at the County Emergency Clinical Hospital Craiova, Romania. A retrospective study of bacterial pathogens was carried out on 90 adult cancer patients admitted from January to December 2018. The analysis of the resistance patterns for the action of the appropriate antibiotics was performed using Vitek 2 Compact system and diffusion method. In this study there were analysed 92 samples from 90 oncological patients (37-86 years). A total of 157 bacterial isolates were obtained, of which 37 strains of Staphylococcus aureus (23.56%), followed by Streptococcus pneumoniae (23- 14.64%), Klebsiella spp. and Escherichia coli (22 - 14,01%). The most common isolates were from respiratory tract (86 isolates - 54.77%). High rates of MDR were found for E. coli (63.63%), MRSA (61,11%) and Klebsiella spp. (54,54%), while one third of the isolated strains of Pseudomonas aeruginosa, Acinetobacter spp. and Proteus spp. were MDR. The findings of this study may be the basis for further more extensive studies highlighting the germs involved in the infectious pathology of cancer patients, in order to determine the antimicrobial resistance and to improve the methods of prophylaxis and treatment. Keywords: multidrug resistance (MDR), cancer patients, bacterial pathogen

scholarly journals Antibiotic Resistance ofSalmonellaspp. Isolated from Shrimp Farming Freshwater Environment in Northeast Region of Brazil

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Fátima C. T. Carvalho ◽  
Oscarina V. Sousa ◽  
Edirsana M. R. Carvalho ◽  
Ernesto Hofer ◽  
Regine H. S. F. Vieira
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Shrimp Farming ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Plasmid Curing ◽  
Freshwater Environment ◽  
Disk Diffusion Method ◽  
Northeast Region ◽  
Drug Resistance Pattern

This study investigated the presence and antibiotic resistance ofSalmonellaspp. in a shrimp farming environment in Northeast Region of Brazil. Samples of water and sediments from two farms rearing freshwater-acclimatedLitopenaeus vannameiwere examined for the presence ofSalmonella. Afterwards,Salmonellaisolates were serotyped, the antimicrobial resistance was determined by a disk diffusion method, and the plasmid curing was performed for resistant isolates. A total of 30 (16.12%) of the 186 isolates were confirmed to beSalmonellaspp., belonging to five serovars:S. serovar Saintpaul,S. serovar Infantis,S. serovar Panama,S. serovar Madelia, andS. serovar Braenderup, along with 2 subspecies:S. entericaserovar houtenae andS. entericaserovar enterica. About twenty-three percent of the isolates were resistant to at least one antibiotic, and twenty percent were resistant to at least two antibiotics. Three strains isolated from water samples (pond and inlet canal) exhibited multiresistance to ampicillin, tetracycline, oxytetracycline, and nitrofurantoin. One of them had a plasmid with genes conferring resistance to nitrofurantoin and ampicillin. The incidence of bacteria pathogenic to humans in a shrimp farming environment, as well as their drug-resistance pattern revealed in this study, emphasizes the need for a more rigorous attention to this area.

scholarly journals The relationship between phylogenetic groups and antibiotic susceptibility patterns of Escherichia coli strains isolated from feces and urine of patients with acute or recurrent urinary tract infection

2020 ◽  
Author(s):  
Hossein Norouzian ◽  
Mohammad Katouli ◽  
Nader Shahrokhi ◽  
Sharam Sabeti ◽  
Mohammad Pooya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Resistance Pattern ◽  
Phylogenetic Groups ◽  
E Coli ◽  
Drug Resistance Pattern ◽  
Biochemical Fingerprinting ◽  
Group D

Background and Objectives: B2 and D have been mentioned as the most common phylogenetic groups among uropatho- genic Escherichia coli. However, there is still controversy about the importance of these phylo-groups. This study was con- ducted to investigate the probable relation between these groups and antibiotic resistance patterns of E. coli isolates derived from urine and feces of the patients with acute or recurrent UTI. Materials and Methods: 10 isolates were recovered from urine and feces samples of patients with different phases of UTI in whom E. coli was causative pathogen. Biochemical fingerprinting was performed to classify the isolates and select their appropriate representatives. Phylogenetic grouping was performed using multiplex PCR, and antibiotic resistance was deter- mined by disk diffusion method. Results: Five-hundred-sixty E. coli isolates were derived from 56 UTI patients (27 acute, 29 recurrent). Among them, 261 isolates were selected using biochemical fingerprinting. All the isolates were sensitive to imipenem and nitrofurantoin. Com- pared to other phylo-groups, the isolates in group D showed considerably different frequencies in acute vs. recurrent phase of UTI, in urine vs. stool samples, in males vs. females, and in- vs. out-patients. They were more resistant to the antibiotics (except norfloxacin), and in contrast to others, this was seen more in acute UTI, especially in urine samples. Multi-drug resistance pattern was also meaningfully higher in group D. Conclusion: Although phylo-groups B2 and D of E. coli bacteria are more responsible for UTI, group D isolates seem to be more resistant and probably more virulent, even than the ones from group B2.

scholarly journals ESTIMATION OF VIBRIO SPECIES INCIDENCES AND ANTIBIOTIC RESISTANCE IN DIARRHEA PATIENTS.

2018 ◽  
Vol 11 (1) ◽  
pp. 369
Author(s):  
Nutan Thakur ◽  
Harish Changotra ◽  
Rahul Shrivastava ◽  
Neelam Grover ◽  
Jitendraa Vashistt
Keyword(s):  
Antibiotic Resistance ◽  
Age Groups ◽  
Vibrio Species ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
High Morbidity ◽  
Distribution Analysis ◽  
Disk Diffusion Method ◽  
Vibrio Spp

 Objectives: Diarrheal diseases are globally important public health concern due to high morbidity and mortality rates among all age groups. Incidences and antimicrobial resistance patterns of Vibrio species are either underreported or overlooked in low-to-middle income countries. The present study is a hospital surveillance conducted to estimate the incidences of Vibrio infections in diarrhea.Methods: A total of 572 diarrheal stool specimens were collected from two major hospitals and investigated using bacteriological tests coupled with molecular assays for characterization of Vibrio species. Kirby-Bauer’s disk diffusion method was utilized to monitor antibiotic resistance against different antibiotic classes.Results: Biochemical and molecular analysis of isolates revealed that Vibrio species accounted for about 5% of diarrheal incidences. Higher rates of Vibrio spp. infections were observed among adolescent and adult age groups than children and elderly. Seasonal distribution analysis showed uniform Vibrio spp. infections throughout the year; however, frequency peaks were observed during rainy seasons. Vibrio spp. showed sensitivity for most antibiotics classes: Quinolones, aminoglycosides, and carbapenems; however, alarming resistance rates were observed against cephalosporins and nalidixic acid.Conclusion: The present study provides credible estimates of Vibrio incidences and resistance pattern in diarrheal patients. Our findings will help in establishing trends in diarrhea etiology and management of severe diarrhea cases.

scholarly journals Antibiotic resistance pattern of Salmonella spp. isolated from stool samples of hospitalized diarrheal patients in Bangladesh

2018 ◽  
Vol 3 (4) ◽  
pp. 534-538 ◽  
Author(s):  
Mohammad Sharif Uddin ◽  
Md Imranul Hoq ◽  
Mohammad Shaokat Ali ◽  
Md Mijanur Rahman ◽  
KM Shariful Islam
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Public Health Problem ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Mortality And Morbidity ◽  
Antibiotic Susceptibility Test ◽  
Drug Resistance Pattern ◽  
Stool Samples

This study was carried out to investigate the drug resistance pattern of Salmonella spp. from diarrheal patients of under 5 years of age. Collected stool samples from the hospitalized diarrheal patients were analyzed for the presence of Salmonella spp. Among 350 stool samples from diarrheal patients, 15 (4%) were positive for the Salmonella spp. Antibiotic susceptibility test of the identified Salmonella spp. was performed according to Kirby-Bauer disc diffusion method. Eight commonly used antibiotics including azithromycin (15 μg), chloramphenicol (30 μg), Sulphamethaxole-trimethoprim (25 μg), metronidazole (50 μg), tetracycline (30 μg), doxycycline (30 μg), erythromycin (15 μg) and ciprofloxacin (5 μg) used to determine drug resistance pattern of the identified Salmonella spp. Majority of the isolates were multidrug resistant showed resistance against more than three drugs. 73% of Salmonella spp. was resistant to 2-4 drugs and 20% showed resistance to more than 5 drugs. We found that 100% Salmonella showed resistance to metronidazole and 87% were resistant to erythromycin. 7% isolates were resistant to chloramphenicol and doxycycline and 20% were resistant to ciprofloxacin. Antibiotic resistance is a serious public health problem worldwide and a leading cause of mortality and morbidity. This kind of situation, leads to great socioeconomic losses from the perspective of the patient, the hospital, and the whole society. To get rid form the drug resistance problem unnecessary, over and misuse of antibiotics should immediately prohibited and policy of judicious use of antibiotics should be strengthened.Asian J. Med. Biol. Res. December 2017, 3(4): 534-538

scholarly journals ESTIMATION OF VIBRIO SPECIES INCIDENCES AND ANTIBIOTIC RESISTANCE IN DIARRHEA PATIENTS.

2018 ◽  
Vol 11 (1) ◽  
pp. 369
Author(s):  
Nutan Thakur ◽  
Harish Changotra ◽  
Rahul Shrivastava ◽  
Neelam Grover ◽  
Jitendraa Vashistt
Keyword(s):  
Antibiotic Resistance ◽  
Age Groups ◽  
Vibrio Species ◽  
Health Concern ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
High Morbidity ◽  
Distribution Analysis ◽  
Disk Diffusion Method ◽  
Vibrio Spp

 Objectives: Diarrheal diseases are globally important public health concern due to high morbidity and mortality rates among all age groups. Incidences and antimicrobial resistance patterns of Vibrio species are either underreported or overlooked in low-to-middle income countries. The present study is a hospital surveillance conducted to estimate the incidences of Vibrio infections in diarrhea.Methods: A total of 572 diarrheal stool specimens were collected from two major hospitals and investigated using bacteriological tests coupled with molecular assays for characterization of Vibrio species. Kirby-Bauer’s disk diffusion method was utilized to monitor antibiotic resistance against different antibiotic classes.Results: Biochemical and molecular analysis of isolates revealed that Vibrio species accounted for about 5% of diarrheal incidences. Higher rates of Vibrio spp. infections were observed among adolescent and adult age groups than children and elderly. Seasonal distribution analysis showed uniform Vibrio spp. infections throughout the year; however, frequency peaks were observed during rainy seasons. Vibrio spp. showed sensitivity for most antibiotics classes: Quinolones, aminoglycosides, and carbapenems; however, alarming resistance rates were observed against cephalosporins and nalidixic acid.Conclusion: The present study provides credible estimates of Vibrio incidences and resistance pattern in diarrheal patients. Our findings will help in establishing trends in diarrhea etiology and management of severe diarrhea cases.

scholarly journals Trends Of Antibiotic Susceptibility Of Salmonella Enterica Serovar Typhi And Paratyphi In An Urban Hospital Of Dhaka City Over 6 Years Period

1970 ◽  
Vol 5 (2) ◽  
pp. 42-45 ◽  
Author(s):  
Khandaker Shadia ◽  
Shajeda Binte Borhan ◽  
Humaira Hasin ◽  
Sharmin Rahman ◽  
Shahin Sultana ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Salmonella Enterica ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Dhaka City ◽  
Urban Hospital ◽  
First Line ◽  
Salmonella Enterica Serovar Typhi ◽  
Antibiotic Resistance Pattern

The antibiotic resistance pattern of salmonella is ever changing over time. The present study is a retrospective analysis of rate of isolation of Salmonella Typhi and Paratyphi and their antibiotic resistance pattern over 6 years period in an urban hospital of Dhaka city. Blood culture submitted in BIRDEM hospital from 2004-2009 were analyzed. Isolated Salmonella sp were identified and antimicrobial susceptibility testing was carried out by a standard disc diffusion method. Among 385 isolated Salmonella sp 304 (79%) were Salmonella enterica serovar Typhi and 81 (21%) were Salmonella enterica serover Paratyphi A. The rate of isolation of S. Paratyphi A has increased over 6 the year period from 14% to 24%. Resistance to individual first line anti-salmonella drugs has increased from 2004 to 2006 (42 to 63%) but has decreased thereafter. Similar pattern was found when simultaneous resistance to three first line antibiotics namely ampicillin, chloramphenicol and co-trimoxazole were considered. Out of total 304 S. Typhi, 117 (38%) were simultaneously resistant to all three first line drugs compared to only 1.8% S. Paratyphi A. Analysis showed that 80 to 90% of isolated S. Typhi was nalidixic acid resistant (NARST) with reduced susceptibility to ciprofloxacin while the rate for S. Paratyphi A was 71-94%. All S. Typhi and Paratyphi A were sensitive to ceftriaxone. The study showed that there was a gradual decline of resistance of S. Typhi to first line antibiotics but very high prevalence of nalidixic acid resistant S. Typhi and S. Paratyphi in Bangladesh. DOI: http://dx.doi.org/10.3329/imcj.v5i2.10097 IMCJ 2011; 5(2): 42-45

Epidemiology of Urinary Tract Infection and Antibiotic Resistance Pattern in Patients Referred to Amiralmomenin Hospital of Gerash City in 2018

2020 ◽  
Author(s):  
Elham Rezaee ◽  
Seyed Adnan Kashfi ◽  
Parisa Bagheri
Keyword(s):  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Age Groups ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Biochemical Tests ◽  
Tract Infections

Background and Aims: Urinary tract infections are one of the most common human infections seen in all age groups and both sexes. Inappropriate use of antibiotics to treat urinary tract infection causes the resistance of the pathogens to the drug. The present study aimed to determine the frequency of gram-negative and gram-positive bacteria and antibiotic resistance patterns in patients with urinary tract infection. Materials and Methods: Samples were cultured on Blood Agar and Eosin Methylene Blue. Colonieschr('39') growth was identified by biochemical tests and standard microbiological and antibiotic sensitivity tests, which were performed with the disc diffusion method according to the Clinical and Laboratory Standards Institute 2016 Standard. Results and Conclusion: The isolated bacteria showed the highest susceptibility to imipenem (89.66%) and meropenem (87.21%) and the highest resistance to sulfamethoxazole (50.00%) and nalidixic acid (44.09%). So, using imipenem is recommended as the most effective antibiotic for the treatment of infection.

Changing patterns and widening of antibiotic resistance inShigellaspp. over a decade (2000–2011), Andaman Islands, India

2014 ◽  
Vol 143 (3) ◽  
pp. 470-477 ◽  
Author(s):  
D. BHATTACHARYA ◽  
H. BHATTACHARYA ◽  
D. S. SAYI ◽  
A. P. BHARADWAJ ◽  
M. SINGHANIA ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Wide Spectrum ◽  
Resistance Pattern ◽  
Third Generation ◽  
Isolation And Identification ◽  
Antimicrobial Drugs ◽  
Drug Resistance Pattern ◽  
Resistance Patterns ◽  
Third Generation Cephalosporins

SUMMARYThis study is a part of the surveillance study on childhood diarrhoea in the Andaman and Nicobar Islands; here we report the drug resistance pattern of recent isolates ofShigellaspp. (2006–2011) obtained as part of that study and compare it with that ofShigellaisolates obtained earlier during 2000–2005. During 2006–2011, stool samples from paediatric diarrhoea patients were collected and processed for isolation and identification ofShigellaspp. Susceptibility to 22 antimicrobial drugs was tested and minimum inhibitory concentrations were determined for third-generation cephalosporins, quinolones, amoxicillin-clavulanic acid combinations and gentamicin. A wide spectrum of antibiotic resistance was observed in theShigellastrains obtained during 2006–2011. The proportions of resistant strains showed an increase from 2000–2005 to 2006–2011 in 20/22 antibiotics tested. The number of drug resistance patterns increased from 13 in 2000–2005 to 43 in 2006–2011. Resistance to newer generation fluoroquinolones, third-generation cephalosporins and augmentin, which was not observed during 2000–2005, appeared during 2006–2011. The frequency of resistance inShigellaisolates has increased substantially between 2000–2006 and 2006–2011, with a wide spectrum of resistance. At present, the option for antimicrobial therapy in shigellosis in Andaman is limited to a small number of drugs.

Sign in / Sign up

Export Citation Format

Share Document