scholarly journals Isolation and Characterization of Hypolipidemic Compound from Cajanus cajan

2011 ◽  
Vol 24 (1) ◽  
pp. 6-10
Author(s):  
M Anwar Habib ◽  
ASM Anisuzzaman ◽  
RK Barman ◽  
M Abdul Gafur ◽  
M Tofazzal Haque
Keyword(s):  
Cajanus Cajan ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Hypolipidemic Activity ◽  
1H And 13C Nmr ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Biological Studies ◽  
Isolated Compound

The study was carried out to identify the compound responsible for hypolipidemic and hypoglycemic effects of Cajanus cajan (redgram). The methanol extract of redgram seeds was found to decrease fasting blood glucose and lipid profile (p<0.001) on streptozotocin-induced mice compared to control. This activity- guided fraction led to the isolation of a compound, substituted benzene containing polyhydroxy functions fused with lactone (CCA3) by analysis of 1H and 13C-NMR data. Biological studies of the isolated compound possessed prominent hypolipidemic activity. Although a number of hypoglycemic compounds are reported, yet not any hypolipidemic compound from redgram. The compound CCA3 seems to be the first report on hypolipidemic activity from methanol extract of redgram. TAJ 2011; 24(1): 6-10

scholarly journals Isolation and Characterisation of Hydrolysable Tannin from Ethyl Acetate Portion of the Aerial Part of Phyllanthus amarus Schum. & Thonn

2019 ◽  
pp. 1-10
Author(s):  
Halima A. Umar ◽  
Hamidu Usman ◽  
Mustapha B. Abubakar ◽  
Baba F. Mohammed ◽  
Mohammed Babakura ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanol Extract ◽  
Phyllanthus Amarus ◽  
Hydrolysable Tannin ◽  
Isolation And Characterization ◽  
Hydrolysable Tannins ◽  
Isolated Compound ◽  
Layer Chromatography

The present work involves extraction of phytochemicals from aerial part of Phyllanthus amarus Schum. & Thonn with n-hexane and 85% methanol. The isolation and characterization of Phytoconstituents was done from the methanol extract through portioning with chloroform and ethyl acetate. Fractionation and isolation (using column and thin layer chromatography respectively) of ethyl acetate column pooled portion afforded a compound coded as E-3.3C. The structure of the isolated compound was established on spectroscopic evidences (IR, 1HNMR, MS), which revealed the compound as 1-de (oxygalloyl)-2ˈ,3ˈ,-di-methoxy-amariin a hydrolysable tannins.

ISOLATION AND CHARACTERIZATION OF ARISTOLOCHIC ACID - I FROM ARISTOLOCHIA INDICA LINN. LEAVES

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (09) ◽  
pp. 52-53
Author(s):  
M.B Mulik ◽  
◽  
K.S. Laddha
Keyword(s):  
Aristolochic Acid ◽  
Chloroform Extract ◽  
Isolation Method ◽  
Aristolochic Acids ◽  
Isolation And Characterization ◽  
Isolated Compound ◽  
Anti Fungal ◽  
Aristolochic Acid I ◽  
Aristolochia Species

Aristolochic Acids (AAs) are major components of plants in Aristolochia species. The plant Aristolochia indica Linn. has diverse biological actions such as analgesic, anti-diabetics, anti-bacterial, anti-fungal and treatment of malaria and fevers. Here, we report a new and simple isolation method of Aristolochic Acid-Ι (AA-I) from leaves of Aristolochia indica Linn. Defatted leaves of A. indica were extracted with chloroform by soxhlation. AA-I was further isolated from the chloroform extract by partitioning with aqueous sodium bicarbonate. AA-I was purified by repetitive washings with methanol and recrystallization in methanol yielded yellow leaflets. The purity of the isolated compound was ascertained by HPLC analysis. structural elucidation of the isolated compound was done by IR, MS and NMR spectral analysis.

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

scholarly journals ISOLATION AND CHARACTERIZATION OF A NOVEL HYALURONIDASE INHIBITOR FROM A MARINE ACTINOMYCETES STRAIN

2015 ◽  
Vol 2 (1) ◽  
pp. 114 ◽  
Author(s):  
Enjuro Harunari ◽  
Chiaki Imada ◽  
Yasuhiro Igarashi ◽  
Takao Fukuda ◽  
Takeshi Terahara ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Tokyo Bay ◽  
Marine Actinomycetes ◽  
Moderate Growth ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Hyaluronidase Inhibitor ◽  
Actinomycete Strain ◽  
Culture Extract

A novel hyaluronidase inhibitor (HI) was isolated from the culture extract of a marine- derived actinomycete strain. This strain MB-PO13 was isolated from ascidian (Molgula manhattensis) in Tokyo Bay. Out of about 1,000 isolates from various marine organisms, strain MB-PO13 had the strongest inhibitory activity and was selected for further study. The strain showed abundant-to-moderate growth on most media, forming a grayish mycelium. On the basis of the taxonomical characteristics, the strain was classified as belonging to the genus of Streptomyces and was named as Streptomyces sp. strain MB-PO13. The structure of HI was elucidated by interpretation of NMR data. HI displayed about 25-fold potent hyaluronidase inhibitory activity against hyaluronidase than glycyrrhizin. Keywords: marine actinomycetes; Streptomyces; hyaluronidase inhibitor 

Two Antinematodal Phenolics from Knema hookeriana, a Sumatran Rainforest Plant

2000 ◽  
Vol 55 (3-4) ◽  
pp. 300-304 ◽  
Author(s):  
Yohannes Alen ◽  
Shuhei Nakajima ◽  
Teruhiko Nitoda ◽  
Naomichi Baba ◽  
Hiroshi Kanzaki ◽  
...  
Keyword(s):  
Effective Dose ◽  
Spectral Properties ◽  
Methanol Extract ◽  
Bursaphelenchus Xylophilus ◽  
Chromatographic Purification ◽  
Pine Wood ◽  
Cotton Ball ◽  
Isolation And Characterization ◽  
First Time

Abstract The activity-guided chromatographic purification of the methanol extract of Knema hookeriana, using pine wood nematodes Bursaphelenchus xylophilus has successfully led to the isolation and characterization of two phenolic antinematodal compounds with minimun effective dose (MED) of 4.5 and 20 μg/cotton ball (μg/ bl.) or 0.018 and 0.073 μᴍ/cotton ball (μᴍ/bl.), respectively. Based on their chemical and spectral properties, these compounds were determined to be 3-undecylphenol (1) and 3-(8Z-tridecenyl)-phenol (2). These compounds were isolated for the first time from this species, and 2 seems to be a novel compound.

Semiquinol and phenol compounds from seven Senecio species

2011 ◽  
Vol 65 (1) ◽  
Author(s):  
Boris Mandić ◽  
Dejan Gođevac ◽  
Ljubodrag Vujisić ◽  
Snežana Trifunović ◽  
Vele Tesević ◽  
...  
Keyword(s):  
Spectroscopic Studies ◽  
1H And 13C Nmr ◽  
Serbia And Montenegro ◽  
Phenol Compounds ◽  
Aerial Parts ◽  
Isolation And Characterization ◽  
Phytochemical Investigation ◽  
Compound Ii ◽  
Tof Ms

AbstractIn this paper, examination of the aerial parts of seven Senecio species from Serbia and Montenegro is reported. Phytochemical investigation of Senecio erucifolius led to the isolation and characterization of semiquinol butyl 2-(1-hydroxy-4-oxocyclohexa-2,5-dienyl)acetate (I), along with methyl 2-(1-hydroxy-4-oxocyclohexa-2,5-dienyl)acetate (jacaranone, II), and methyl 2-(4-hidroxyphenyl)acetate (III). The structure of I was established based on spectroscopic studies (1H- and 13C-NMR, IR, and CI-MS). Compound II was also isolated from S. carpathicus and S. subalpinus. The presence of jacaranone in the methanol extracts of S. wagneri, S. othonnae, and S. paludosus was confirmed by LC/ESI-TOF MS.

Preparation, isolation, and characterization of permethylated galactosides and fucosides

1999 ◽  
Vol 77 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Daniel D Asres ◽  
Hélène Perreault
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Characterization Methods ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Layer Chromatography ◽  
Structural Aspects

Sugar analysis involving permethylation followed by methanolysis can lead to significant results, given that permethylated monosaccharide standards are available for comparison of spectral and chromatographic data. Such standards are not readily commercially available, especially the furanoside forms. This note describes the isolation and characterization of permethylated pyranoside and furanoside species of D(+)-galactose and L(-)-fucose. Separation of the isomers was optimized using a combination of column chromatography and continuous elution thin-layer chromatography (TLC). TLC, gas chromatography - mass spectrometry, and 1H nuclear magnetic resonance (NMR) spectroscopy were used as the characterization methods. The isolation of furanosides is emphasized, since no specific NMR data have been reported on those to date, while several reports have already discussed the structural aspects of pyranosides.Key words: permethylation, monosaccharide, GC-MS, TLC, NMR.

scholarly journals Isolation and Characterization of Stigmasterol from Fritillaria roylei

2020 ◽  
Vol 9 (2) ◽  
pp. 77-80
Author(s):  
Gunpreet Kaur ◽  
Vikas Gupta ◽  
R G Singhal ◽  
Parveen Bansal
Keyword(s):  
Spectral Analysis ◽  
Melting Point ◽  
Methanol Extract ◽  
Analytical Techniques ◽  
Chemical Marker ◽  
Marker Compound ◽  
Isolation And Characterization ◽  
Single Compound ◽  
Isolated Compound ◽  
First Time

Fritillaria roylei (Kshirakakoli) is the threatened species of “Ashtwarga” group suffers lot of confusion for identification & authentification in Ayurvedic system of medicine. Due to lack of natural sources and insufficient availability of kshirakakoli, chances of adulteration and substitution increases which in turn leads to loss of faith of people in herbal drugs. Thus for identification and differentiation, quality standardization and quality assurance of kshirakakoli containing herbal formulations there is a need to isolate chemical marker compound using advanced analytical techniques. The methanol extract of root samples of plant was prepared and phytochemical screening was performed. Marker compound was isolated from the extract using column chromatography. Single compound having Rf value 0.31 was isolated with TLC by using mobile phase n-hexane: ethyl acetate: formic acid (8:2:0.1 v/v/v) and purified by re-crystallization with methanol. Isolated compound was further characterized by using melting point and spectral analysis. The methanol extract was dark brown in color and showed the presence of steroids, amino acids and flavonoids. The isolated compound was found to be white crystalline powder with melting point range of 167-169°C. Spectral analysis confirmed the presence of Stigmasterol. In present study stigmasterol was isolated for the first time and can be used as chemical marker for identification and differentiation of the plant from its substitutes.

scholarly journals Co(III) and VO(IV) complexes with a new bidentate Schiff base: Interaction with BSA and antimicrobial studies

2019 ◽  
Vol 9 (1) ◽  
pp. 3776-3782 ◽  
Keyword(s):  
Schiff Base ◽  
Analytical Techniques ◽  
Molar Conductance ◽  
Base Interaction ◽  
1H And 13C Nmr ◽  
Pyramidal Geometry ◽  
Antimicrobial Studies ◽  
Biological Studies ◽  
Bacteria And Fungi

A Schiff base ligand L was prepared from the condensation of 2-amino-3-benzyloxypyridine and 5-chlorosalicylaldehyde. The ligand forms complexes with Co(III) and VO(IV) in good yield. Design, synthesized and characterization of cobalt(III) and oxidovanadium(IV) with (E)-2-((3-(benzyloxypyridinylimino) methyl)-4-chlorophenol (L) are described. The prepared complexes were characterized by different analytical techniques elemental analysis, molar conductance, infrared spectra, 1H and 13C NMR, mass, electronic absorption and TGA. The coordination geometry is octahedral in C1 and C2, while complexes C3 and C4 show a square-pyramidal geometry. Numerous biological studies have been conducted on L and Co(III) and VO(IV) complexes. The results of antimicrobial studies indicated that all compounds had antimicrobial activity against bacteria and fungi. The binding of metal complexes with BSA (bovine serum albumin) was reported. The results of these indicated that Co(III) and VO(IV) are significantly quenching fluorescence intensity of BSA, that results in changing its conformation and results in a static quenching.

Isolation and Characterization of Cytotoxic Diterpenoid Isomers from Propolis

1997 ◽  
Vol 52 (9-10) ◽  
pp. 702-704 ◽  
Author(s):  
Tetsuya Matsuno ◽  
Yasuyuki Matsumoto ◽  
Masahiro Saito ◽  
Junji Morikawa
Keyword(s):  
Methanol Extract ◽  
Carcinoma Cell ◽  
Molecular Formula ◽  
Cell Cytotoxicity ◽  
Isolation And Characterization ◽  
Clerodane Diterpenoids ◽  
Human Hepatocellular Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell ◽  
Brazilian Propolis

The methanol extract of Brazilian propolis was fractionated by HPLC, based on HuH13 (human hepatocellular carcinoma) cell cytotoxicity assay. Two isomers of diterpenoid with a molecular formula of C20H30O3 (MW: 318.46) were isolated. The structures of these colorless compounds were determined as clerodane diterpenoids (I, 15-oxo-3, 13Z-kolavadien-17-oic acid; II, 15-oxo-3Z, 13E-kolavadien-n-oic acid)

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