Effect of the clathrate complex of selenopyran and β-cyclodextrin on the rate of healing of a conditionally aseptic full-thickness planar wound in rats

Introduction. Selenopyran is an organic selenium compound with sharply hydrophobic properties. An increase in solubility in water (and as a consequence – and bioavailability) is possible due to the formation of inclusion complexes with cyclodextrins.Aim. The aim of this work was to study the effect of a gel containing a clathrate complex of selenopyran with β-cyclodextrin on the rate of wound healing on a model of a conditionally aseptic full-thickness planar wound in rats.Materials and methods. The object of the study was a gel containing a clathrate complex of selenopyran with β-cyclodextrin (the content of selenopyran in the gel was 0.1 %). A model of a full-thickness planar wound in sexually mature male rats was used. 20 individuals were divided into 2 groups – intact (without treatment) and experimental (received gel treatment). Efficacy was assessed by the change in wound area at 3, 5, 7, 9, 11 and 14 days after application of wound.Results and discussion. The results of the study showed that the relative area of the wounds in the treated animals by the 3rd day of the experiment was less than in the intact ones. On the fifth day of the experiment, the differences were statistically significant (57.49 ± 12.51 % in treated animals versus 85.27 ± 26.61 % in intact animals). By the 14th day of the experiment, there were practically no differences in the groups of animals.Conclusion. The results obtained indicate that when using a gel containing selenopyran in combination with β-cyclodextrin, it accelerates the transition from the inflammation phase to the proliferation phase. This is most likely due to the antioxidant properties of selenopyran. Considering the lower concentration of selenopyran in comparison with the therapeutic concentrations of other antioxidants (taurine, allantoin), it can be considered as a promising wound healing agent for further study.

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EFEK SALEP EKSTRAK PINANG TERHADAP LEVEL FIBROBLAST DAN KOLAGEN PADA PROSES PENYEMBUHAN LUKA

JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan ◽

10.22437/jmj.v7i1.7121 ◽

2019 ◽

Vol 7 (1) ◽

pp. 19-25

Author(s):

Ave Olivia Rahman ◽

Humaryanto Humaryanto

Keyword(s):

Wound Healing ◽

Male Rats ◽

Control Group ◽

Betel Nut ◽

Full Thickness ◽

Wound Area ◽

Treatment Groups ◽

Areca Catechu ◽

Collagen Level ◽

Full Thickness Wound

ABSTRACT Background: Some plants, such as betel nuts (Areca catechu), is used as traditional antiseptic. Betel nut mash is applied on ulcus wound. Fibroblast and collagen are important factor in wound healing. Research objective : this study is to determine the effect of betel nuts extract on fibroblast and collagen level in full thickness wound healing processs. Methodology: this study used male rats Sprague dawney that randomly divided into 3 groups. Groups I received no treatment, groups II and III received areca catechu extracts with concentration 15% and 30% respectively. There are 12 rats in each groups. The treatment is given every day without wound’s debridement. Incision were made full thickness with diameter size 1,5 cm, on right back skin was made by lidocain anesthesia subcutaneously. Wound area were measured every days. Termination of rats were done in day 7 and day 16 to histopathology assessment with Haematoxylin-Eosin stain for fibroblast and collagen level by semiqualitative score. Results: level of collagen were higher in group that received extract but level of fibroblast were lower than control group in histopathology of day 7th. Conclusion: Extract of betel nut increased level of collagen. Keyword : areca catechu, betel nuts, wound, fibroblast, collagen, histopathology ABSTRAK Latar belakang : Beberapa tanaman digunakan sebagai antiseptik luka, salah satunya biji pinang untuk penyembuhan luka ulkus. Fibroblast dan kolagen merupakan salah satu faktor penting dalam penyembuhan luka. Tujuan : Penelitian ini bertujuan mengetahui efek ekstrak biji pinang terhadap level fibroblast dan kolagen pada proses penyembuhan luka full thickness Metode : Penelitian ini menggunakan tikus galur Sprague dawney jantan berusia 2-3 bulan dan telah mendapatkan persetujuan etik. Setelah aklimatisasi, tikus dibagi secara acak masing-masing 12 ekor dalam 3 kelompok, yaitu kelompok I tanpa perlakuan, kelompok II diberikan salep ekstrak biji pinang 15%, kelompok III diberikan salep ekstrak biji pinang 30%. Luka full thickness dibuat dengan diameter 1,5 cm di daerah punggung belakang bagian kanan dengan anestesi lidokain subkutan. Perlakuan dilakukan setiap hari tanpa debridemen luka. Luas luka diukur setiap hari. Terminasi dilakukan pada hari ke 7 dan 16 untuk pemeriksaan histopatologi jaringan luka dengan pengecatan Haematoxylin-Eosin. Skoring secara semikualitatif untuk menilai fibroblast dan kolagen. Hasil : Pada kelompok ekstrak pinang terdapat peningkatan level kolagen, akan tetapi tidak terjadi peningkatan level fibroblast pada hasil histopatologi luka kulit hari ke-7. Kesimpulan : terjadi peningkatan level kolagen pada pemberian ekstrak biji pinang. Kata kunci : biji pinang, luka, histopatologi, fibroblast, kolagen

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The Effect of 17β-Estradiol Administration on Cutaneous Wound Healing in 24-Week Ovariectomized Female Mice

Journal of Hormones ◽

10.1155/2014/234632 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Kanae Mukai ◽

Yukari Nakajima ◽

Tamae Urai ◽

Emi Komatsu ◽

Kana Takata ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Cutaneous Wound Healing ◽

Full Thickness ◽

Estrogen Replacement ◽

Wound Area ◽

Cutaneous Wound ◽

Female Mice ◽

Exogenous Estrogen ◽

17Β Estradiol

Estrogen replacement promotes cutaneous wound healing in 8–10-week young ovariectomized female mice. However, research using aged ovariectomized female mice has not been reported, to the best of our knowledge. Therefore, we investigated the effect of 17β-estradiol on cutaneous wound healing using 24-week middle-aged ovariectomized female mice. Twenty-week-old female mice were divided into three groups: medication with 17β-estradiol after ovariectomy (OVX + 17β-estradiol), ovariectomy (OVX), and sham (SHAM). After 4 weeks, the mice received two full-thickness wounds. Then, the OVX + 17β-estradiol group was administered 17β-estradiol at 0.01 g/day until healing. The ratio of wound area in the OVX + 17β-estradiol group was significantly decreased compared with that in the OVX group. The numbers of neutrophils and macrophages in the OVX + 17β-estradiol group were significantly smaller than those in the OVX group. In addition, the ratio of myofibroblasts in the OVX + 17β-estradiol group was significantly higher than that in the OVX group. These data suggested that exogenous continuous 17β-estradiol administration promotes cutaneous wound healing in 24-week OVX female mice by reducing wound area, shortening inflammatory response, and promoting wound contraction. However, it is unclear whether the effect of exogenous estrogen on wound healing outweighs the delay of wound healing due to advanced age.

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Investigation of the bischofite gel reparative effect on the linear wound model

Research Results in Pharmacology ◽

10.3897/rrpharmacology.4.30697 ◽

2018 ◽

Vol 4 (3) ◽

pp. 87-93 ◽

Cited By ~ 1

Author(s):

Alena Mayorova ◽

Boris Sysuev ◽

Isita Kchanaliyeva ◽

Ekaterina Tretyakova ◽

Snezhana Evseeva ◽

...

Keyword(s):

Wound Healing ◽

Male Rats ◽

Control Group ◽

Calorimetric Analysis ◽

Wound Area ◽

Healing Effect ◽

Wound Model ◽

Significant Difference ◽

Morphological Picture ◽

Reparative Effect

Introduction. The currently available information on the bischofite mineral pharmacodynamics makes it possible to assume that bischofite-based gels have wound-healing properties. Materials and methods. Experiments were performed on 36 male rats. Using a blade, a linear wound of 50 mm long was modeled. Animals were divided into 4 groups: 1) Control; 2) Bishofit (500 mg of gel with bischofite on the wound area for 7 days); 3) Actovegine (500 mg of Actovegine gel on the wound area for 7 days); 4) Contractubex (500 mg of contractubex gel on the wound area for 7 days). The wound healing effect of the drugs was evaluated through studying the physicomechanical properties, assessing the morphological picture, determining the concentration of hydroxyproline (HP) and calculating the ratio of types I and III collagen. Results. The strongest healing effect on the wound defect, significantly greater than that in the control (p<0.01), was obtained in the group that received bischofite gel (13.70±0.76 N). Actovegine also demonstrated a positive reparative effect (12.60±0.63 N, p<0.05). Significantly lower (p<0.01) healing effect than in the control group was obtained in the group of animals that had received contractubex (9.65±0.59 N). In the calorimetric analysis, it was found that the highest concentration of HP was in the tissues of wound defects in animals treated with contractubex; however, there was no statistically significant difference with the control group. Significantly lower in comparison with the control (p<0.05) concentration of HP, was found in tissues of simulated wounds in animals treated with bischofite gel (79.7% of the control). When assessing the ratio of types I and III collagen in the tissues of the wound defect, when stained with picrosirius red, it was found that by the number of mature collagen fibers, the studied groups can be arranged in the following descending order: Bishofite> Actovegine> Control> Contractubex. A similar trend is demonstrated by the morphological picture of tissues in the area of the wound defect. Conclusion. The study showed that the best results were obtained with external use of bischofite gel. Actovegine has a less significant, but quite pronounced reparative, effect on this model. The least satisfactory results were obtained when applying contractubex.

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Jaundice in second intention wound healing in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502004000300003 ◽

2004 ◽

Vol 19 (3) ◽

pp. 176-185

Author(s):

Maria de Lourdes Pessole Biondo-Simões ◽

Celso Fernando Ribeiro Araújo ◽

João Henrique Felício de Lima ◽

Marcelo Ferreira ◽

Marcos de Abreu Bonardi

Keyword(s):

Wound Healing ◽

Skin Wound ◽

Biochemical Changes ◽

Male Rats ◽

Second Step ◽

Collagen Concentration ◽

Skin Wound Healing ◽

Wound Area ◽

Histopathologic Analysis ◽

The Ideal

PURPOSE: The objective of the present study was to monitor the process of second intention skin wound healing in rats with jaundice. METHODS: The study was divided into two steps. In the first, obstructive jaundice was induced in 68 male rats to investigate the biochemical changes and to determine the ideal time for the study of healing. In the second step, 54 rats were divided into two groups: control (normal) and experimental (jaundiced). Seventy-two hours after jaundice induction, a standardized wound was produced in the dorsum by removing the skin and the subcutaneous net, whose contraction was assessed on the 3rd, 7th, 10th and 14th day and monitored with a computer program. After sacrifice, the tissue was submitted to histopathologic analysis. RESULTS: The most marked biochemical changes were detected between the 3rd and 5th day. The reduction of he wound area was slower in the jaundice group at all 4 time points studied (p3=0.0366, p7=0.0054, p10=0.0000. p14=0.0000). Collagen concentration was lower in the wound of jaundiced animals (p3=0.0000. p7=0.0000. p14=0.0000). CONCLUSION: Jaundice delays collagen deposition and maturation and wound contraction in wounds left to heal by second intention wound healing in rats.

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Comparison of the Cutaneous Wound Healing of Ovariectomized Mouse at 12 Weeks with That of SHAM and Estrogen-Administered Mice

Journal of Hormones ◽

10.1155/2014/484258 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Kanae Mukai ◽

Yuriko Miyasaka ◽

Kana Takata ◽

Tamae Urai ◽

Yukari Nakajima ◽

...

Keyword(s):

Wound Healing ◽

Estradiol Level ◽

Cutaneous Wound Healing ◽

Full Thickness ◽

Wound Area ◽

Cutaneous Wound ◽

Cutaneous Wounds ◽

Female Mice ◽

Ovariectomized Mouse ◽

Significant Difference

The aim of this experiment was to investigate the effect of 17β-estradiol on cutaneous wound healing in 12-week ovariectomized (OVX) female mice. Eight-week-old female mice were divided into three groups: administration of 17β-estradiol after OVX (OVX + 17β-estradiol), OVX, and sham (SHAM). Four weeks after surgery, the mice received two full-thickness cutaneous wounds. 17β-Estradiol at 0.01 g/day was administered on the backs of mice in the OVX + 17β-estradiol group every day. Plasma 17β-estradiol level in the OVX + 17β-estradiol group was thus significantly higher than in the SHAM and OVX groups, but there was no significant difference between SHAM and OVX groups. The ratio of wound area was not significantly different among the three groups. However, the period required to reach a ratio of wound area of 0.15 in the OVX + 17β-estradiol group was significantly shorter than in the SHAM and OVX groups. These results indicate that cutaneous wound healing in young OVX mice was promoted by the administration of 17β-estradiol compared with that in SHAM and OVX mice without such administration, but there was no difference between the latter two groups that did not differ in 17β-estradiol level.

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Investigation of Healing Effects of Cinnamic Acid in a Full-Thickness Wound Model in Rabbit

Jundishapur Journal of Natural Pharmaceutical Products ◽

10.5812/jjnpp.97669 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Mohammad Naghavi ◽

Pari Tamri ◽

Sara Soleimani Asl

Keyword(s):

Wound Healing ◽

Cinnamic Acid ◽

Wound Closure ◽

Antioxidant Properties ◽

Skin Wound ◽

Hair Follicles ◽

Vehicle Group ◽

Full Thickness ◽

Skin Wound Healing ◽

Tissue Samples

Background: Wounds represent a major global problem for health care systems, clinicians, patients, and their families. Cinnamic Acid (CA) is a naturally occurring phenolic compound that possesses anti-inflammatory, antimicrobial, and antioxidant properties. Objectives: This study aimed to investigate the effects of CA on skin wound-healing in the animal model. Methods: Full-thickness wounds were created on the back of white New Zealand rabbits of both sexes. Animals were divided into six groups (six animals and 12 wounds in each group). Negative control received no treatment, while positive control was treated with phenytoin cream, vehicle group with eucerin, and test groups with 0.1, 1, and 10% CA ointments. The healing activity of CA was evaluated by determining the wound closure rate and hydroxyproline content of wound tissue samples. In addition, the histopathological study of tissue samples of different groups was performed using hematoxylin and eosin staining. Results: The rate of wound closure and hydroxyproline levels of tissue samples in animals treated with CA 0.1% were significantly (P < 0.05) higher than those of no-treatment and vehicle-treated groups. Histological study revealed the increased number of fibroblasts and hair follicles, increased reepithelialization rate, and enhanced neovascularization in CA 0.1%-treated group when compared to no-treatment and vehicle groups. Conclusions: Cinnamic acid at low concentrations (< 1%) is potent for skin wound-healing and could be used as a safe and effective topical healing agent. Further studies are needed to confirm our findings.

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Comparison Of The Efficacy Of Two Laser Types In Healing Of Full-Thickness Wound: An Experimental Study

Russian Open Medical Journal ◽

10.15275/rusomj.2020.0418 ◽

2020 ◽

Vol 9 (4) ◽

Author(s):

Hesham Galal Mahran

Keyword(s):

Wound Healing ◽

High Intensity ◽

Male Rats ◽

Control Group ◽

Full Thickness ◽

Scanning Method ◽

Low Intensity ◽

Laser Group ◽

Group B ◽

Full Thickness Wound

The aim of the study ― To investigate the effectiveness of pulsed laser with high intensity and laser with low intensity on a full-thickness wound in rats and to compare the effects of both laser types. Material and Methods ― Forty-five adult male rats were utilized in the present study. After anesthesia, wound of five cm2 was produced on dorsal aspect in each rat; then, the rats were randomly classified into three groups as follows: high power pulsating laser group (A) which received Nd: YAG laser with 1064 nm wavelength, low power laser group (B) which received GaAlAs laser with 830 nm wavelength, and control group (C) which received no treatment. Laser lights in groups (A) and (B) were introduced at an intensity of five joules per cm2 with a total energy of 25 joules for each wound area through the use of a scanning method and at a rate of 3 sessions per week for three weeks. Results ― At the end of treatment, better wound healing in form of more wound contraction and higher healing rate was elicited in both laser groups in comparison to wound healing in the control group, and there were no significantly different effects between both laser types. Conclusion ― At the intensity of five joules/cm2, both low-intensity and pulsating high-intensity lasers speed up the healing of the wound in rats, and there are no significant differences between their effects.

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Effects of Three Types of Japanese Honey on Full-Thickness Wound in Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/504537 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 11

Author(s):

Yukari Nakajima ◽

Yuki Nakano ◽

Sono Fuwano ◽

Natsumi Hayashi ◽

Yukiho Hiratoko ◽

...

Keyword(s):

Wound Healing ◽

Combined Treatment ◽

Full Thickness ◽

Wound Area ◽

Proliferative Phase ◽

Inflammatory Phase ◽

Hydrocolloid Dressing ◽

Thickness Skin ◽

Full Thickness Wound ◽

Buckwheat Flour

Although many previous studies reported that honey promotes wound healing, no study has examined the effects of Japanese honey. The aim of this study was to investigate the effects of three types of Japanese honey, Acacia, Buckwheat flour, and Chinese milk vetch honey, on wound healing in comparison with hydrocolloid dressing. Circular full-thickness skin wounds were produced on male mice. Japanese honey or hydrocolloid dressing was applied daily to the mice for 14 days. The ratio of wound area for the hydrocolloid dressing group increased initially in the inflammatory and early proliferative phases and then decreased rapidly to heal with scarring. However, the ratios of wound area for the Japanese honey groups decreased in the inflammatory phase, increased in the proliferative phase, and decreased in the proliferative phase, and some wounds were not completely covered with new epithelium. These findings indicate that using Japanese honey alone has limited benefit, but since it reduces wound size in the inflammatory phase, it is possible to apply a combined treatment in which Japanese honey is applied only in the inflammatory phase, followed by hydrocolloid dressing from the proliferative phase, which would effectively contract the wound.

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Healing Effects of Iranian Dwarf Elder on Full-thickness Epidermal Thermal Injury in Wistar Rats

Annals of Military and Health Sciences Research ◽

10.5812/amh.121231 ◽

2022 ◽

Vol 19 (4) ◽

Author(s):

Hosein Mohamadi haftador ◽

Parisa Ramhormozi ◽

Mitra Yousefpour ◽

Ali Sobhanizadeh ◽

Laya Ghahari

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Wistar Rats ◽

Thermal Injury ◽

Herbal Medicines ◽

Vehicle Group ◽

Control Group ◽

Full Thickness ◽

Wound Area ◽

Burn Wounds

Background: Wound healing is a physiologic process that cells and tissues react when the skin is exposed to thermal injury. The inflammatory process has ‎essential effects on wound healing that includes coordination between immunological and biological responses. Chemical and herbal medicines are locally or systemically administrated to help wound healing. Herbal medicines have become more prevalent in recent years due to fewer side effects. Dwarf elder is used to treating inflammation and has been reported to possess antibacterial and antioxidant activities. This plant is also effective against burns, wounds, eczema, and infection. Objectives: In this study, we evaluated the topical application of dwarf elder on full-thickness epidermal thermal wounds in Wistar rats. Methods: Thirty adult male Wistar rats of about 300 g were used. The rats were randomly divided into three groups: Control group, vehicle group treated with an ointment base, and treatment group treated with elder ointment 10%. The animals were anesthetized and were created burn wounds with an area of 1 cm2 using the Meyer method. After 21 days, the wounds were photographed with a Canon camera. The wound area and healing degree were calculated. Slides were stained with H&E and examined by light microscopy and GraphPad Prism software. Data were analyzed by SPSS software using ANOVA and Tukey’s test. The significance level was considered at P < 0.05. Results: The wound area on the 21st day was significantly lower in the treatment group than in the control and vehicle groups. The epithelium thickness (μm) was significantly higher in the treatment group than in the control and vehicle groups. The blood vessels were significantly higher in the treatment group than in the other groups. Conclusions: The study showed that elder extract could be an effective remedy to repair and heal thermal injury and burn wounds.

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