scholarly journals Endophthalmitis Caused by Curtobacterium pusillum Following Open Globe Injury

2021 ◽  
Vol 62 (9) ◽  
pp. 1292-1299
Author(s):  
Sang Yoon Kim ◽  
Tae Hoon Kim ◽  
Sang Joon Lee ◽  
Han Jo Kwon
Keyword(s):  
Rural Areas ◽  
Primary Closure ◽  
Visual Disturbance ◽  
Identification System ◽  
Beta Lactam ◽  
Open Globe Injury ◽  
Microbial Identification ◽  
Subretinal Neovascularization ◽  
First Case ◽  
Open Globe

Purpose: To report the first case of Curtobacterium endophthalmitis within 6 hours after open globe injury, with extensive phlebitis and secondary subretinal neovascularization. Case summary: A 53-year-old man with hypersensitivity to beta-lactam antibiotics was admitted due to visual disturbance in the left eye experienced while working in a rural area. Fundus examination was impossible due to a full-layer corneal laceration and traumatic cataract in the left eye. B-scan ultrasonography and orbital computed tomography showed no shadowing of retained intraocular foreign bodies. After a corneal scraping smear, primary closure, lensectomy, and vitrectomy were performed. Organic material was observed in a focal area of pale macula, accompanied by extensive retinal phlebitis in the mid-periphery. After diagnosing acute bacterial endophthalmitis, intravitreal vancomycin and dexamethasone were injected. Curtobacterium pusillum was cultured on an automated microbial identification system. Intravenous vancomycin and oral clarithromycin were administered for 2 weeks. After 3 months, endophthalmitis had not recurred, and the visual acuity reached 20/100. However, subretinal neovascularization was newly detected under the damaged macula. No complications of neovascularization were observed until 6 months after primary closure. Conclusions: Curtobacterium pusillum can induce acute endophthalmitis through direct penetration in cases of ocular trauma, and may be accompanied by extensive phlebitis and secondary subretinal neovascularization. In cases of open globe injury sustained in rural areas, acute endophthalmitis caused by a rare Gram-positive bacillus, such as Curtobacterium species, should be considered.

scholarly journals Identification of Mycobacterium neoaurumIsolated from a Neutropenic Patient with Catheter-Related Bacteremia by 16S rRNA Sequencing

2000 ◽  
Vol 38 (9) ◽  
pp. 3515-3517 ◽  
Author(s):  
Patrick C. Y. Woo ◽  
Hoi-Wah Tsoi ◽  
Kit-Wah Leung ◽  
Peggy N. L. Lum ◽  
Andy S. P. Leung ◽  
...  
Keyword(s):  
16S Rrna ◽  
Lymphoblastic Leukemia ◽  
Similarity Index ◽  
16S Rrna Sequencing ◽  
Identification System ◽  
Rrna Gene ◽  
Microbial Identification ◽  
First Case ◽  
Rrna Sequencing ◽  
Mycobacterial Strain

A rapidly growing pigmented mycobacterial strain with an ambiguous biochemical profile was isolated from the blood culture taken through the Hickman catheter of a 9-year-old girl with acute lymphoblastic leukemia. Whole-cell fatty acid analysis showed that the best match profile was that of Mycobacterium aurum, but the similarity index was only 0.217, meaning that there were no good matches between the isolate and the organisms in the database of the Microbial Identification System. The 16S rRNA gene of the mycobacterial strain was amplified, agarose gel purified, and sequenced. There were 44 base differences between the gene sequence of the isolate and that ofM. aurum but only one base difference between the sequence of the isolate and that of Mycobacterium neoaurum, showing that the isolate was indeed a strain of M. neoaurum by using this “gold standard.” This represents the first case ofM. neoaurum infection documented by 16S rRNA sequencing.

scholarly journals Identification of Three Strains of Mycobacterium Species Isolated from Clinical Samples Using Fatty Acid Methyl Ester Profiling

2003 ◽  
Vol 31 (2) ◽  
pp. 133-140 ◽  
Author(s):  
A Ozbek ◽  
O Aktas
Keyword(s):  
Fatty Acid ◽  
Methyl Esters ◽  
Acid Methyl Ester ◽  
Cellular Fatty Acid ◽  
Clinical Samples ◽  
Identification System ◽  
Cyclopropane Fatty Acid ◽  
Mycobacterium Species ◽  
Microbial Identification ◽  
Acid Methyl

The cellular fatty acid profiles of 67 strains belonging to three different species of the genus Mycobacterium were determined by gas chromatography of the fatty acid methyl esters, using the MIDI Sherlock® Microbial Identification System (MIS). The species M. tuberculosis, M. xenopi and M. avium complex were clearly distinguishable and could be identified based on the presence and concentrations of 12 fatty acids: 14:0, 15:0, 16:1ω7c, 16:1ω6c, 16:0, 17:0, 18:2ω6,9c, 18:1ω9c, 18:0, 10Me-18:0 tuberculostearic acid, alcohol and cyclopropane. Fatty acid analysis showed that there is great homogeneity within and heterogeneity between Mycobacterium species. Thus the MIS is an accurate, efficient and relatively rapid method for the identification of mycobacteria.

Outcomes of Penetrating Keratoplasty After Open Globe Injury

Cornea ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Katie X. Li ◽  
Asad F. Durrani ◽  
Yunshu Zhou ◽  
Peter Y. Zhao ◽  
Bradford L. Tannen ◽  
...  
Keyword(s):  
Penetrating Keratoplasty ◽  
Open Globe Injury ◽  
Open Globe

The first study on the bacterial flora of the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)

Biologia ◽  
2006 ◽  
Vol 61 (6) ◽  
Author(s):  
Hüseyin Yilmax ◽  
Kazım Sezen ◽  
Hatice Kati ◽  
Zihni Demirbağ
Keyword(s):  
Bark Beetle ◽  
Micrococcus Luteus ◽  
Bacterial Flora ◽  
Biochemical Properties ◽  
Identification System ◽  
Dendroctonus Micans ◽  
Microbial Identification ◽  
Spruce Bark Beetle ◽  
Spruce Bark ◽  
European Spruce Bark Beetle

AbstractThe European spruce bark beetle, Dendroctonus micans Kugelann (Coleoptera, Scolytidae), is one of the most serious pests of oriental spruce (Picea orientalis L.) in Turkey. In this study, we investigated bacterial flora of D. micans collected from different populations of the forests of Eastern Black Sea Region of Turkey from 2002 to 2004. Seven different bacteria were isolated from healthy, diseased and dead specimens based on the color of colony and morphology. According to morphological, physiological and biochemical properties, metobolic enyzme profile by BIOLOG microtiter plate system, and total cellular fatty acid profile by Microbial Identification System (MIS), isolates were identified as Micrococcus luteus, Bacillus thuringiensis subsp. morrisoni, Serratia grimesii, Enterobacter cloaceae, Enterobacter intermedius, Streptococcus sp. and Pseudomonas putida. This is the first study on the bacterial flora of D. micans.

scholarly journals An Uncommon Meridional Globe Rupture due to Blunt Eye Trauma

2018 ◽  
Vol 2018 ◽  
pp. 1-3 ◽  
Author(s):  
Krishan Kumar ◽  
Rick Figurasin ◽  
Swati Kumar ◽  
Muhammad Waseem
Keyword(s):  
Ocular Trauma ◽  
Severe Form ◽  
Open Globe Injury ◽  
Unique Case ◽  
Pertinent Literature ◽  
Eye Trauma ◽  
Globe Rupture ◽  
Open Globe ◽  
Blunt Eye Trauma ◽  
Blunt Ocular Trauma

Open globe injury (OGI) is a severe form of eye trauma. It is an important cause of monocular blindness worldwide. Ruptures from blunt trauma are most common at the sites where the sclera is thinnest, at the insertions of the extraocular muscles, and at the limbus. Most often, rupture is equatorial. We present a unique case of open globe injury due to blunt ocular trauma from a thrown rock that resulted in a meridional rupture of the eye. The pertinent literature is reviewed.

scholarly journals First Report of a Fruit Rot of Pumpkin Caused by Acidivorax avenae subsp. citrulli in Georgia

Plant Disease ◽  
1999 ◽  
Vol 83 (2) ◽  
pp. 199-199 ◽  
Author(s):  
D. B. Langston ◽  
R. D. Walcott ◽  
R. D. Gitaitis ◽  
F. H. Sanders
Keyword(s):  
Polyclonal Antibodies ◽  
Pcr Amplification ◽  
Tween 80 ◽  
Soft Rot ◽  
Fruit Rot ◽  
Identification System ◽  
Banding Patterns ◽  
First Report ◽  
Microbial Identification ◽  
Necrotic Spots

In September 1998, a fruit rot was reported affecting pumpkin (Cucurbita pepo) in a commercial field in Terrell Co., Georgia. Symptoms on the surface of fruit occurred as round, necrotic spots or cracks a few millimeters in diameter. With age, the tissue surrounding these lesions became soft and wrinkled. A soft rot expanded into the flesh of the pumpkin, originating from the lesions observed on the surface. In time, infected pumpkins totally collapsed. V-shaped, necrotic lesions occurred at the margin of the leaf and extended inward toward the mid-rib. Samples were collected from the field and bacteria were isolated from fruit and leaf lesions onto King's medium B (1). The bacterium isolated was rod shaped, gram negative, nonflourescent, oxidase positive, Tween 80 positive, carboxymethyl cellulose positive, β-OH butyrate positive, and malonate negative. The bacterium reacted positively with polyclonal antibodies specific for the watermelon fruit blotch pathogen Acidivorax avenae subsp. citrulli and was identified as A. avenae subsp. citrulli by MIDI (Microbial Identification System, Newark, DE) according to statistical analysis of fatty acid data. Results from polymerase chain reaction (PCR) amplification of the bacterium isolated from pumpkin yielded 360-bp fragments that, when digested with the restriction enzyme HaeIII, had DNA banding patterns identical to those of stock A. avenae subsp. citrulli DNA. Koch's postulates were completed successfully with 2-week-old watermelon seedlings. This is the first report of A. avenae subsp. citrulli causing fruit rot of pumpkin in Georgia. Reference: (1) E. O. King et al. J. Lab. Clin. Med. 44:301, 1954.

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