QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
GITA MISHRA ◽  
HEMESHWER KUMAR CHANDRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Reducing Sugars ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Family ◽  
Ethanolic Extracts ◽  
Antioxidant Evaluation ◽  
In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species- Endemic plant species of Western Ghats

Biomedicine ◽  
2021 ◽  
Vol 40 (4) ◽  
pp. 460-466
Author(s):  
Vinayak Lokapur ◽  
Varsha Jayakar ◽  
Manjula Shantaram
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Western Ghats ◽  
Skin Diseases ◽  
Antioxidant Properties ◽  
Physicochemical Analysis ◽  
Phytochemical Analysis ◽  
Qualitative And Quantitative ◽  
In Vitro Antioxidant Activity

Introduction and Aim: Plants from Anacardiaceae family are used in indigenous system of medicine for their anti-arthritic, antibacterial and antioxidant properties. Among them, Holigarna species are widely known as toxic members of Anacardeaceae family which are endemic to Western Ghats. It is commonly known as allergic plant which causes contact dermatitis if contacted. However, young leaves and seeds are used against haemorrhoids, obesity, and cancer and skin diseases. Despite the claims and the use of Holigarna ferrugenia, Holigarna nigra and Holigarna grahamii for the management of skin diseases in traditional medicine, little is known and documented so the aim of the study was to explore the in vitro antioxidant activity and to analyse qualitative and quantitative of phytochemicals along with their physicochemical properties. Materials and Methods: Physicochemical parameters such as moisture, pH, total ash, acid-insoluble ash, and elemental values were determined. Qualitative and quantitative analysis were carried out along with different antioxidant assays for evaluating the antioxidant activity of aqueous crude extract of H. ferrugenia, H. nigra and H. grahamii. Results: Physicochemical analysis revealed the presence of trace elements. Preliminary phytochemical analysis revealed the presence of tannins, alkaloids, flavonoids, phenols, saponins and steroids in aqueous crude extract of H. ferrugenia, H. nigra and H. grahamii. The free radical scavenging activity of all plants exhibited better antioxidant properties. Conclusion: The results indicated that the leaves contain an appreciable number of bioactive compounds and presence of these phytochemicals, especially the phenols and flavonoids could be the reason behind the use of both plants in ethno-medicine for the management of various ailments.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

scholarly journals Induction, biochemical trait and phytochemical screening of calluses of Amburana cearensis (Allemão) A.C. Smith

2020 ◽  
Vol 42 ◽  
pp. e54187
Author(s):  
Jéssica Nascimento Costa Vascocelos ◽  
Alone Lima Brito ◽  
Andressa Priscila Pianco Santos Lima ◽  
Jackson Roberto Guedes da Silva Almeida ◽  
Ana Paula de Oliveira ◽  
...  
Keyword(s):  
Growth Curve ◽  
High Performance ◽  
Reducing Sugars ◽  
Dichlorophenoxyacetic Acid ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Total Proteins ◽  
Biochemical Tests ◽  
2,4 Dichlorophenoxyacetic Acid

Amburana cearensis is an arboreal legume of the Fabaceae family, with high phytotherapic and medicinal potential due the presence of secondary metabolites. The objective of this study was to evaluate the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-amino-2,5,6- trichloro-2-pyridinecarboxylic acid (picloram) on the in vitro induction of callogenesis of A. cearensis and analyze the biochemical and phytochemical potential of these calluses. For callus induction, leaf and cotyledon segments were used as explants, which were inoculated in woody plant medium (WPM) supplemented with different concentrations of 2,4-D (0, 5, 10, 20, 40 μM) or picloram (0, 5, 10, 20, 40, 80 μM). The callus growth curve was estimated based on fresh weight, measured at 7-day intervals until 28 days after inoculation. The calluses were analyzed by biochemical tests to quantify the reducing sugars and total proteins. Phytochemical screening and high-performance liquid chromatography were performed to establish the phytochemical profile of extracts from calluses. The concentrations of 21.94 μM and 26.46 μM of 2,4-D induced the greatest formation of compact and friable calluses from the leaf and cotyledon segments, respectively. The growth curve had two distinct phases (lag and exponential) for both types of calluses evaluated. The maximum levels of reducing sugars and total proteins in the calluses from leaf and cotyledon segments were obtained on the day of inoculation and after 28 days of cultivation, respectively. The results of the phytochemical analysis identified the presence of coumarin in all the extracts evaluated, this secondary metabolite has high pharmacological potential.

scholarly journals Evaluation of the antibacterial activity on strains responsible for diarrhoea; antioxidant and cytotoxic activities in vitro of the leaves and trunk bark of Vernonia conferta Benth (Asteraceae)

2021 ◽  
Vol 17 (1) ◽  
pp. 041-051
Author(s):  
Ivan Cédric Mvondo Ozela ◽  
Patrick Yamen Mbopi ◽  
Herve Narcisse Bayaga ◽  
Pierre Réne Kwetche Fotsing ◽  
Jean Jacques Tchouani ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Raw Material ◽  
Dilution Method ◽  
Cytotoxic Activities ◽  
Phytochemical Screening ◽  
Traditional Medicines ◽  
Ethanolic Extracts ◽  
Emergence Of Resistance

The high incidence of infectious diseases and the emergence of resistance to modern drugs are current public health concerns. This situation leads to the search for alternatives via medicinal plants. The objective of this study was to assess the properties of Vernonia conferta. We performed an experimental study. The plant material consisted of the leaves and trunk bark of V. conferta. The extraction was carried out by maceration using aqueous and hydro-ethanolic solvents, followed by phytochemical screening of the extracts. An evaluation of the antibacterial potential was carried out by the micro-dilution method and was followed by the evaluation of the antioxidant properties (DPPH and FRAP test) and cytotoxicity (resazurin staining) of the extracts. The E2 and E4 extracts exhibited better extraction yields. The phytochemical screening noted the presence of families of compounds in common (polyphenols, tannins and quinones) and those which are different. The hydro-ethanolic extracts exhibited antibacterial activity on the strains of interest with MICs varying from 2 to 32 mg / mL with a possibility of synergistic action between the compounds. The evaluation of the antioxidant properties showed that the extracts E1, E3 and E4 showed better properties with IC50 = 25.1 ± 0.410; 2.456 ± 0.002; 2.363 ± 0.015 µg / mL respectively according to the tested method. The extracts showed their non-cytotoxicity with CC50> 1000 µg / mL. The activities of hydro-ethanolic extracts of V. conferta suggest that the latter would be a potential raw material for the production of improved traditional medicines.

scholarly journals The The Phytochemical Screening, Total Phenolic Contents and Antioxidant Activities in Vitro of White Oyster Mushroom (Pleurotus Ostreatus) Preparations

2019 ◽  
Vol 7 (15) ◽  
pp. 2404-2412
Author(s):  
Santun Bhekti Rahimah ◽  
Dhiah Dianawaty Djunaedi ◽  
Arto Yuwono Soeroto ◽  
Tatang Bisri
Keyword(s):  
Antioxidant Activity ◽  
Pleurotus Ostreatus ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Ethanolic Extracts ◽  
Active Substances

BACKGROUND: The popular commercially cultivated Pleurotus ostreatus mushroom contains very high nutrients and bioactive compounds with high antioxidant activity. The ethanolic extract seems to be the most active in preparation. AIM: This study has an aim to compare the phytochemical analysis of a fresh, dry and ethanolic extract of Pleurotus ostreatus, to measure the total phenolic content and antioxidant activities in vitro of ethanolic extracts of Pleurotus ostreatus. METHODS: The fresh plant's materials (FPM), dry plants materials (DPM), ethanolic extracts were macerated with 70% (EE70) and 96% ethanol (EE96) of Pleurotus ostreatus which were used for phytochemical analysis, and EE96 was used for antioxidant activity in vitro. The phytochemical analysis was conducted using the Dragendorf and Meyer, FeCl3 test, Salkowsky method, Lieberman method, amyl alcohol, foam test and the NaOH reagent. The total phenol test was carried out using the Follin-Ciocalteu method. The antioxidant activity was tested using the ABTS and H2O2 essay. RESULTS: The phytochemical screening showed that the flavonoid, phenolic compounds, tannin, saponin, alkaloids, and steroids were detected in the FPM, DPM, EE70 and also the EE96. The alkaloid, however, was not identified by the Meyer Reagent in the FPM and DPM. The DPM and EE70 seemed to have the highest amount of saponin based on the foam that was formed. Meanwhile, steroids and flavonoids were detected at a higher level in the EE96, based on the strength of visible colour. However, triterpenoid and quinones could not be identified. In the total phenol test, there was an amount of 6.67 μg phenol in a 1 mg extract sample which was equivalent to 1 mg of Gallic Acid. The EE96 has an IC50 of 108.07 μg/mL for ABTS and an IC50 reduction of 229.17 μg/mL. The process of Pleurotus ostreatus drying did not reduce the content of active substances. The polar active substances seem to be more soluble in the EE70 than the EE96. CONCLUSION: The higher the bioactive substances in the preparation, the more significant the bio-therapeutic effects. Ethanolic extract of Pleurotus ostreatus has a phenol content and a good antioxidant action.  

scholarly journals PHARMACOGNOSTICAL, PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF HYDROMETHANOL EXTRACT OF GORDONIA DIPTEROSPERMA KURZ.

2020 ◽  
pp. 35-40
Author(s):  
PHAIBIANG LAPASAM ◽  
ANDREW LALTHASANGA ◽  
FREDDY TEILANG NONGKHLAW ◽  
ZOTHAN PUIA
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Water Soluble ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Pharmacologically Active

Objective: This research is to evaluate the pharmacognostic parameter, phytochemical analysis, and in vitro antioxidant properties of hydromethanol extract. Method: This study carries out the collection and authentication of the plant, extraction, pharmacognostic study, preliminary phytochemical screening, and antioxidant property of leaves hydromethanol extract were evaluated by scavenging the following free radicals – DPPH (2,2-diphenyl- 1-picrylhydrazyl), hydrogen peroxide, nitric oxide, and reducing power. One-way analysis of variance (ANOVA) followed by Dunnett’s test was performed. The minimum value of *p˂0.05 considered as significant, **p˂0.01 and ***p˂0.001. Results: Powder microscopy of the leaves showed the presence of stomata, calcium oxalate crystals, trichome, fibers, and oil glands. The total ash was considered to be 3.75%, water-soluble ash 1.25%, and acid-soluble ash 3%. Hydromethanol (3:7) extract yielded 38.8%, moisture content 15.6%. Preliminary phytochemical screening of the extract showed the presence of carbohydrates, glycoside, saponin, phenol, tannin, flavonoid, and steroid. The total flavonoid content was considered to be 32.25 mg/g of quercetin and the total phenolic content of the extract was found 610 mg/g of gallic acid. The IC50 radical scavenging effect of extract and gallic acid was considered to be 3.62 and 3.46 for 2,2-diphenyl-1-picrylhydrazyl, 10.4 and 24.73 for hydrogen peroxide, and 48.76 and 58.83 for nitric oxide. Conclusion: The phytochemical constituents of the extract were well-known pharmacologically active chemicals and significant antioxidant potential was shown by the extract. This study finds out the rationality of the use of this plant as a medicinal plant. Further studies would be needed to explore their potential as a treatment for fever, diarrhea, and dysentery.

Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

2017 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
Michael Russelle Alvarez ◽  
Paolo Robert Bueno ◽  
Raymond Oliver Cruz ◽  
Richard Macapulay ◽  
Francis Jayson Vallesfin ◽  
...  
Keyword(s):  
Crude Extract ◽  
Carica Papaya ◽  
Uv Light ◽  
Digestive Enzyme ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Salivary Amylase ◽  
Garcinia Mangostana ◽  
Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. ­Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested ­positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

scholarly journals Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

2018 ◽  
pp. 1-11
Author(s):  
Iserhienrhien Lucky Osafanme ◽  
Okolie Paulinus Ngozi
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

scholarly journals Phytochemical Screening and Biological Studies of Shilajit (Asphaltum)

2017 ◽  
Vol 9 (1) ◽  
pp. 15
Author(s):  
Shahid Aziz ◽  
Sidra Khaliq ◽  
Habib Ur-Rehman ◽  
Kh. Shakeel Ghani ◽  
Muhammad Irshad ◽  
...  
Keyword(s):  
Microbial Activity ◽  
Cardiac Glycosides ◽  
Reducing Sugars ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Biological Studies ◽  
Dead Plant ◽  
Micro Organisms

<p>Shilajit (asphaltum)  is produced  by the long term humification  of dead plant material  and organic vegetable matter  by different micro-organisms and has great potential for the treatment of a variety of human conditions.  This treatise reviews its origin, sources, chemical composition, biological  and  commercial importance. Phytochemical analysis was done by standard methods to evaluate  different Shilajit (asphaltum) classes of compounds in different samples of shilajit  which are responsible for their  biological activity.  Shilajit`s anti-microbial activity has been evaluated  against four different bacterial strains viz., <em>Escherichia coli, Psuedomonas aeuroginosa</em>, <em>Klebisella pneumonia</em> and <em>Staphylococcus aureus. </em>Phytochemical analysis illustrated  that shilajit contains  terpenoids, cardiac glycosides, saponins and reducing sugars. Surprisingly,  some classes of compounds are absent in shilajit  viz., alkaloids, flavonoids, tannins and anthraquinones. . Shilajit showed no response towards halophytic bacteria and  negligible activity was shown towards other strains of bacteria. Since   anti-microbial activity is based on environmental factors  its activity varied  between locations.</p>

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