scholarly journals Rapid quantification of Psilocybin with reversed-phase HPLC and single-wavelength detection

2021 ◽  
Author(s):  
Alexzander Samuelsson ◽  
Eric Janusson ◽  
Sajni Shah ◽  
Markus Roggen
Keyword(s):  
High Throughput ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
New Methods ◽  
Purity Analysis ◽  
Hplc Technique ◽  
Wavelength Detection ◽  
Analytical Requirements ◽  
Rapid Quantification

The alkaloid psilocybin (4-phosphoryloxy-N,N-dimethyltryptamine) and the neurologically active psilocin (4-hydroxy-N,N-dimethyltryptamine) are the foremost compounds of pharmaceutical interest in Psilocybe mushrooms. As these compounds are infrequently analyzed in analytical labs, validated methods for rapid purity analysis are lacking. Newfound therapeutic use has invigorated academic and commercial interests in the molecules and new methods of production and available products are expanding. As a result, high-throughput methods of analysis for psilocybin must be improved to promptly determine chemical differences between mushroom genera or other sources of psilocybin and psilocin, as well as refined product purity. To address this, we developed an inexpensive HPLC technique for the efficient quantification of psilocybin and psilocin by using readily available equipment and dilute reagents. Aqueous ammonium formate (0.143 mM) was found to be preferable over techniques with much higher buffer concentrations or stronger acids for controlling psilocybin Zwitterion resolution. The chromatographic run time satisfied high-throughput analytical requirements with an efficient total runtime under 2 minutes. A standard octadecyl silica (C18) column provided excellent resolution between psilocybin and psilocin signals. The quality of the method was validated using certified analytical reference standards and was found to be accurate (3.5% bias, Psilocybin), reliable (0.32% RSD), and efficient (Psilocybin k’ = 1.78).

scholarly journals Rapid Quantification of Gene Expression by Competitive RT-PCR and Ion-Pair Reversed-Phase HPLC

BioTechniques ◽  
1996 ◽  
Vol 20 (2) ◽  
pp. 250-257 ◽  
Author(s):  
Amanda Hayward-Lester ◽  
Peter J. Oefner ◽  
Peter A. Doris
Keyword(s):  
Gene Expression ◽  
Reversed Phase ◽  
Ion Pair ◽  
Reversed Phase Hplc ◽  
Rt Pcr ◽  
Rapid Quantification

scholarly journals A Validated Reversed-Phase HPLC Analytical Method for the Analysis of Fenofibrate in Bulk Drug and Tablet Dosage Formulation

2021 ◽  
pp. 306-312
Author(s):  
Awdhut Pimpale ◽  
Rajendra Kakde ◽  
Ishwar Kakde
Keyword(s):  
Solvent System ◽  
Reversed Phase ◽  
Pharmaceutical Sciences ◽  
Reversed Phase Hplc ◽  
System Suitability ◽  
Water Ph ◽  
Hplc Technique ◽  
Bulk Drug ◽  
Tablet Dosage ◽  
Dosage Formulation

Aims: A accurate, precise, and stability-indicating Reversed-Phase HPLC technique has been established for the estimation of fenofibrate in tablet formulation. Study Design:  Experimental study. Place and Duration of Study: Department of Pharmaceutical Sciences, RTM Nagpur University, Nagpur-440033, Maharashtra, India between June 2019 and March 2020. Methodology: The chromatographic separation was attained on RP Princeton column (C18) (250 mm x 4.6 mm, 5 µ) with mobile solvent system as a mixture of water (pH 3.0 along o-phosphoric acid) and acetonitrile in the proportion (40:60) v/v, flow rate 1.0 ml per minute, at 240 nm. The retention time of fenofibrate was 3.905 minutes. Results: The method demonstrated linearity in the concentration range of 87-232 µg/ml with a coefficient of correlation (r2) of 0.9994. The % RSD was ˂2% and percentage recovery was found to be 99.13-100.74%. The assay of marketed tablet formulations was found to be 99.98%. Conclusion: The developed and validated technique as per ICH rules for specificity, accuracy, precision, linearity, and system suitability. Reverse Phase-HPLC technique was utilized to the market formulation.

Determination of Flavonoids in Licorice Using Acid Hydrolysis and Reversed-Phase HPLC and Evaluation of the Chemical Quality of Cultivated Licorice

Planta Medica ◽  
2009 ◽  
Vol 76 (07) ◽  
pp. 729-733 ◽  
Author(s):  
Makio Shibano ◽  
Kazuo Ozaki ◽  
Hitoshi Watanabe ◽  
Akinori Tabata ◽  
Masahiko Taniguchi ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Reversed Phase ◽  
Chemical Quality ◽  
Reversed Phase Hplc

Boilerplate

2012 ◽  
Author(s):  
Margaret Jane Radin
Keyword(s):  
Economic Efficiency ◽  
Analytical Framework ◽  
Daily Basis ◽  
Regulatory Agencies ◽  
Jury Trial ◽  
Comprehensive Treatment ◽  
New Methods ◽  
And Control ◽  
Fine Print

Boilerplate—the fine-print terms and conditions that we become subject to when we click “I agree” online, rent an apartment, or enter an employment contract, for example—pervades all aspects of our modern lives. On a daily basis, most of us accept boilerplate provisions without realizing that should a dispute arise about a purchased good or service, the nonnegotiable boilerplate terms can deprive us of our right to jury trial and relieve providers of responsibility for harm. Boilerplate is the first comprehensive treatment of the problems posed by the increasing use of these terms, demonstrating how their use has degraded traditional notions of consent, agreement, and contract, and sacrificed core rights whose loss threatens the democratic order. This book examines attempts to justify the use of boilerplate provisions by claiming either that recipients freely consent to them or that economic efficiency demands them, and it finds these justifications wanting. It argues that our courts, legislatures, and regulatory agencies have fallen short in their evaluation and oversight of the use of boilerplate clauses. To improve legal evaluation of boilerplate, the book offers a new analytical framework, one that takes into account the nature of the rights affected, the quality of the recipient's consent, and the extent of the use of these terms. It goes on to offer possibilities for new methods of boilerplate evaluation and control, and concludes by discussing positive steps that NGOs, legislators, regulators, courts, and scholars could take to bring about better practices.

scholarly journals A Reversed-phase HPLC Study of the Complexation of Benzene Derivatives Guest Molecules with 5,17-bis(N-Tolyliminomethyl)-25,27-dipropoxycalix[4]arene in Acetonitrile–Water Solution

1999 ◽  
Vol 23 (1) ◽  
pp. 60-61
Author(s):  
O. I. Kalchenko ◽  
A. V. Solovyov ◽  
J. Lipkowski ◽  
V. I. Kalchenko
Keyword(s):  
Stability Constants ◽  
Water Solution ◽  
Reversed Phase ◽  
Benzene Derivatives ◽  
Reversed Phase Hplc ◽  
Guest Molecules ◽  
Hplc Study

Stability constants of the host–guest complexes of 5,17-bis( N-tolyliminomethyl)-25,27-dipropoxycalix[4]arene with benzene derivatives were determined by reversed-phase HPLC in acetonitrile–water solution.

scholarly journals Dental Education Challenges during the COVID-19 Pandemic Period in Italy: Undergraduate Student Feedback, Future Perspectives, and the Needs of Teaching Strategies for Professional Development

Healthcare ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 454
Author(s):  
Giuseppe Varvara ◽  
Sara Bernardi ◽  
Serena Bianchi ◽  
Bruna Sinjari ◽  
Maurizio Piattelli
Keyword(s):  
Undergraduate Student ◽  
Teaching And Learning ◽  
Dental Education ◽  
Student Feedback ◽  
Categorical Variables ◽  
Practical Training ◽  
Learning Approaches ◽  
New Methods ◽  
E Learning

The COVID-19 pandemic literally stopped most human movement and activities as it initially spread, which included dental practices and dental education. This defined the need for significative changes in teaching and learning with the use of “e-learning” methods, also for traineeships. This study was designed to determine the undergraduate student perception of these new methods as part of their education. This involved 353 students attending the Dental School of the G. D’Annunzio University of Chieti–Pescara, from the first to the sixth years. A questionnaire in Italian and was set-up using “Google Forms” and sent by email to the students. The questionnaire was divided into three parts: the first part included questions for general information, including age, sex and year of course; the second part had multiple choice questions related to their evaluation of the e-learning teaching, using a scale of opinion in the replies to each question (e.g., “scarce”, “fair”, “satisfying”, “very good” and “excellent”); and the third part included two open questions to indicate the strengths and limitations of these new teaching and learning approaches. The categorical variables in the first and second parts of the questionnaire were evaluated using Chi squared tests, setting significance at p < 0.05, while the comments were evaluated qualitatively. The student feedback showed significant appreciation (p < 0.05) of the new methods and the efforts that the lecturers put in to provide lectures of as high a quality as possible. However, a lack of practical training was significantly perceived as an important problem in the structure of their new curriculum (p < 0.05). COVID-19 has been an epic tragedy that has hit the human population not only in terms of health and healthcare, but also quality of life. This includes the quality of dental education within universities. However, the pandemic can be seen to also represent motivation to invest in the necessary technological innovation to deliver the best possible education to our future dentists.

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