scholarly journals Rapid Separation of Human Hemoglobin on a Large Scale From Non-clarified Bacterial Cell Homogenates Using Molecularly Imprinted Composite Cryogels

Author(s):  
Solmaz Hajizadeh ◽  
Karin Kettisen ◽  
Leif Bülow ◽  
Lei Ye

The production of a macroporous hydrogel column, known as cryogel, has been scaled up (up to 150 mL) in this work for the purification of human hemoglobin from non-clarified bacterial homogenates. Composite cryogels were synthesized in the presence of adult hemoglobin (HbA) to form a molecularly imprinted polymer (MIP)network where the affinity sites for the targeted molecule were placed directly on an acrylamide cryogel by protein imprinting during the cryogelation. The MIP composite cryogel column was first evaluated in a well-defined protein mixture. It showed high selectivity toward HbA in spite of the presence of serum albumin. Also, when examined in complex non-clarified E. coli cell homogenates, the column showed excellent chromatographic behavior. The binding capacity of a 50 mL column was thus found to be 0.88 and 1.2 mg/g, from a protein mixture and non-clarified cell homogenate suspension, respectively. The recovery and purification of the 50 mL column for separation of HbA from cell suspension were evaluated to be 79 and 58%, respectively. The MIP affinity cryogel also displayed binding and selectivity toward fetal Hb (HbF) under the same operational conditions.

2021 ◽  
Vol 54 (3) ◽  
pp. 1-33
Author(s):  
Blesson Varghese ◽  
Nan Wang ◽  
David Bermbach ◽  
Cheol-Ho Hong ◽  
Eyal De Lara ◽  
...  

Edge computing is the next Internet frontier that will leverage computing resources located near users, sensors, and data stores to provide more responsive services. Therefore, it is envisioned that a large-scale, geographically dispersed, and resource-rich distributed system will emerge and play a key role in the future Internet. However, given the loosely coupled nature of such complex systems, their operational conditions are expected to change significantly over time. In this context, the performance characteristics of such systems will need to be captured rapidly, which is referred to as performance benchmarking, for application deployment, resource orchestration, and adaptive decision-making. Edge performance benchmarking is a nascent research avenue that has started gaining momentum over the past five years. This article first reviews articles published over the past three decades to trace the history of performance benchmarking from tightly coupled to loosely coupled systems. It then systematically classifies previous research to identify the system under test, techniques analyzed, and benchmark runtime in edge performance benchmarking.


2021 ◽  
Vol 11 (6) ◽  
pp. 2551
Author(s):  
Hyobum Lee ◽  
Hangseok Choi ◽  
Soon-Wook Choi ◽  
Soo-Ho Chang ◽  
Tae-Ho Kang ◽  
...  

This study demonstrates a three-dimensional numerical simulation of earth pressure balance (EPB) shield tunnelling using a coupled discrete element method (DEM) and a finite difference method (FDM). The analysis adopted the actual size of a spoke-type EPB shield tunnel boring machine (TBM) consisting of a cutter head with cutting tools, working chamber, screw conveyor, and shield. For the coupled model to reproduce the in situ ground condition, the ground formation was generated partially using the DEM (for the limited domain influenced by excavation), with the rest of the domain being composed of FDM grids. In the DEM domain, contact parameters of particles were calibrated via a series of large-scale triaxial test analyses. The model simulated tunnelling as the TBM operational conditions were controlled. The penetration rate and the rotational speed of the screw conveyor were automatically adjusted as the TBM advanced to prevent the generation of excessive or insufficient torque, thrust force, or chamber pressure. Accordingly, these parameters were maintained consistently around their set operational ranges during excavation. The simulation results show that the proposed numerical model based on DEM–FDM coupling could reasonably simulate EPB driving while considering the TBM operational conditions.


2009 ◽  
Vol 87-88 ◽  
pp. 80-85
Author(s):  
Jin Yang Yu ◽  
Xiao Ling Hu ◽  
Cui Cui Jiao ◽  
Ya Mei Zhao ◽  
Wei Wei Yang

Molecularly imprinted composite membranes for selective binding and permeation of roxithromycin were prepared by means of thermal initiated co-polymerization method using polysulfone ultra-filtration membranes as porous supports. Scanning electron microscope was utilized to visualize surface and cross-sections of the membranes to gain more better understanding in the analysis of imprinted layers deposited on PSF support membranes and differential scanning calorimetric was used for determining the thermal stability of the membranes. Static equilibrium binding and recognition properties of the imprinted and non-imprinted membranes to roxithromycin and its analogues in ethanol solution system were tested. The results showed that saturated binding capacity of imprinted membrane to roxithromycin was about 2.24μmol/g, higher than those of its analogues, and the selectivity factors of αRM/EM, αRM/AM and αRM/EE were 1.75, 2.46 and 2.67, respectively. The transport performances of the membranes were evaluated through kinetic filtration experiments. The separation mechanism of the roxithromycin imprinted membrane could be defined as facilitated permeation mechanism.


Author(s):  
Lissett Barrios ◽  
Stuart Scott ◽  
Charles Deuel

The paper reports on developmental research on the effects of viscosity and two phases, liquid–gas fluids on ESPs which are multi stage centrifugal pumps for deep bore holes. Multiphase viscous performance in a full-scale Electrical Submersible Pump (ESP) system at Shell’s Gasmer facility has been studied experimentally and theoretically. The main objectives is to predict the operational conditions that cause degradations for high viscosity fluids when operating in high Gas Liquid Radio (GLR) wells to support operation in Shell major Projects. The system studied was a 1025 series tandem WJE 1000. The test was performed using this configuration with ten or more pump stages moving fluids with viscosity from 2 to 200 cP at various speed, intake pressure and Gas Void Fractions (GVF). For safety considerations the injected gas was restricted to nitrogen or air. The ESP system is a central artificial lift method commonly used for medium to high flow rate wells. Multiphase flow and viscous fluids causes problems in pump applications. Viscous fluids and free gas inside an ESP can cause head degradation and gas locking. Substantial attempts have been made to model centrifugal pump performance under gas-liquid viscous applications, however due to the complexity this is still a uncertain problem. The determination of the two-phase flow performance in these harmful conditions in the ESP is fundamental aspects in the surveillance operation. The testing at Shell’s Gasmer facility revealed that the ESP system performed as theoretical over the range of single flowrates and light viscosity oils up to Gas Volume Fractions (GVF) around 25%. The developed correlations predict GVF at the pump intake based on the operational parameters. ESP performance degrades at viscosity higher than 100cp as compared to light oil applications, gas lock condition is observed at gas fraction higher than 45%. Pump flowrate can be obtained from electrical current and boost for all range of GVF and speed. The main technical contributions are the analysis of pump head degradation under two important variables, high viscosity and two-phase flow inside the ESP.


Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2221
Author(s):  
Joseph W Lowdon ◽  
Hanne Diliën ◽  
Bart van Grinsven ◽  
Kasper Eersels ◽  
Thomas J. Cleij

The scope of the presented research orientates itself towards the development of a Molecularly Imprinted Polymer (MIP)-based dye displacement assay for the colorimetric detection of the antibiotic amoxicillin in aqueous medium. With this in mind, the initial development of an MIP capable of such a task sets focus on monolithic bulk polymerization to assess monomer/crosslinker combinations that have potential towards the binding of amoxicillin. The best performing composition (based on specificity and binding capacity) is utilized in the synthesis of MIP particles by emulsion polymerization, yielding particles that prove to be more homogenous in size and morphology compared to that of the crushed monolithic MIP, which is an essential trait when it comes to the accuracy of the resulting assay. The specificity and selectivity of the emulsion MIP proceeds to be highlighted, demonstrating a higher affinity towards amoxicillin compared to other compounds of the aminopenicillin class (ampicillin and cloxacillin). Conversion of the polymeric receptor is then undertaken, identifying a suitable dye for the displacement assay by means of binding experiments with malachite green, crystal violet, and mordant orange. Once identified, the optimal dye is then loaded onto the synthetic receptor, and the displaceability of the dye deduced by means of a dose response experiment. Alongside the sensitivity, the selectivity of the assay is scrutinized against cloxacillin and ampicillin. Yielding a dye displacement assay that can be used (semi-)quantitatively in a rapid manner.


2018 ◽  
Vol 78 (8) ◽  
pp. 1704-1714 ◽  
Author(s):  
François-René Bourgeois ◽  
Frédéric Monette ◽  
Daniel G. Cyr

Abstract To develop a better understanding for fixed biomass processes, the development of a nitrifying bacterial biofilm, as well as the performance of treatment during modifications to operational conditions of a full-scale submerged biological filter were examined. The development of the nitrifying biofilm was investigated at four depth levels (1, 2, 4 and 5 feet). The result of bacterial subpopulations analyzed by qPCR relative to the physico-chemical parameters of the wastewater during the various tests (sustained aeration, modified backwash parameters and inflow restriction) revealed an increase of the relative presence of nitrifying microorganisms throughout the biofilm (especially for nitrite oxidizing bacteria (NOB)), but this was not necessarily accompanied by a better nitrification rate. The highest observed nitrification rate was 49% of removal in the test cell during backwashing conditions, whereas the relative ammonia oxidizing bacteria (AOB) population was 0.032% and NOB was 0.008% of the total biomass collected. The highest percentage of nitrifying bacteria observed (0.034% AOB and 0.18% NOB) resulted in a nitrification rate of 21%. The treatment of organic matter determined by measuring the chemical and biochemical oxygen demand (COD, CBOD5) was improved.


Author(s):  
Charles H. O. Lombard ◽  
Daniel N. J. Els ◽  
Jacques Muiyser ◽  
Albert Zapke

South Africa’s coal-fired power stations use super heated steam to drive generator turbines. In arid regions, air-cooled condensers (ACCs) are used to condense the process steam. These ACCs consists of an array of over 200 axial flow fans, each driven by a motor via a reduction gearbox. Distorted fan inlet air flow conditions cause transient blade loading, which results in variations in output shaft bending and torque. A measurement project was conducted where the input and output shaft of such a gearbox were instrumented with strain gauges and wireless bridge amplifiers. Gearbox shaft speed and vibration were also measured. Torsional and bending strains were measured for a variety of operational conditions, where correlations were seen between gearbox loading and wind conditions. The input side experienced no unexpected loads from the motor or changing wind conditions, whereas output shaft loading was influenced by the latter. Digital filters were applied to identify specific bending components, such as the influence of fan hub misalignment and dynamic blade loading. Reverse loading of the gearbox was measured during the fan stop period, and vibration analysis revealed torsional and gearbox vibrations. This investigation documented reliable full scale ACC gearbox loads.


2021 ◽  
Vol 8 (3) ◽  
Author(s):  
S. Mamman ◽  
F. B. M. Suah ◽  
M. Raaov ◽  
F. S. Mehamod ◽  
S. Asman ◽  
...  

In this study, a unique magnetic molecularly imprinted polymer (MMIP) adsorbent towards bisphenol A (BPA) as a template molecule was developed by bulk polymerization using β-cyclodextrin (β-CD) as a co-monomer with methacrylic acid (MAA) to form MMIP MAA–βCD as a new adsorbent. β-CD was hybridized with MAA to obtain water-compactible imprinting sites for the effective removal of BPA from aqueous samples. Benzoyl peroxide and trimethylolpropane trimethacrylate were used as the initiator and cross-linker, respectively. The adsorbents were characterized by Fourier transform infrared spectroscopy, scanning electronic microscopy, transmission electron microscopy, vibrating sample magnetometer, Brunauer–Emmett–Teller and X-ray diffraction. 1 H nuclear magnetic resonance spectroscopy was used to characterize the MAA–βCD and BPA–MAA–βCD complex. Several parameters influencing the adsorption efficiency of BPA such as adsorbent dosage, pH of sample solution, contact time, initial concentrations and temperature as well as selectivity and reusability study have been evaluated. MMIP MAA–βCD showed significantly higher removal efficiency and selective binding capacity towards BPA compared to MMIP MAA owing to its unique morphology with the presence of β-CD. The kinetics data can be well described by the pseudo second-order kinetic and Freundlich isotherm and Halsey models best fitted the isotherm data. The thermodynamic studies indicated that the adsorption reaction was a spontaneous and exothermic process. Therefore, MMIP based on the hybrid monomer of MAA–βCD shows good potential of a new monomer in molecularly imprinted polymer preparation and can be used as an effective adsorbent for the removal of BPA from aqueous solutions.


Mission critical events are changing operational conditions that will have a significant impact on the mission. If they are specified correctly, one can begin to design meaningful crew station responses. This chapter is about how to make decisions that are appropriate for the environment; in this case, under increased time compression. The theoretical focus of decisions shifts the conceptual design of the decision analytic structure forward to the problem definition stage. In large-scale dynamic systems, getting the problem right is often the most difficult task of the operator and operational manager. Operational decision making (ODM) stands in visible contrast to conventional decision making, and conventional decision theory, in that among all classes of decisions, an operational decision is singular, and contains a number of unique components.


Sign in / Sign up

Export Citation Format

Share Document