scholarly journals Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device

Author(s):  
Hongrang Chen ◽  
Haitao Zhang ◽  
Yun Shen ◽  
Xingliang Dai ◽  
Xuanzhi Wang ◽  
...  

Background: This study aims to design a 3D printed handheld electrospinning device and evaluate its effect on the rapid repair of mouse skin wounds.Methods: The device was developed by Solidworks and printed by Object 350 photosensitive resin printer. The polylactic acid (PLA)/gelatin blend was used as the raw material to fabricate in-situ degradable nanofiber scaffolds. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and water vapor permeability test were used to evaluate the material properties of the scaffolds; cytotoxicity test was performed to evaluate material/residual solvent toxicity, and in situ tissue repair experiments in Balb/c mouse were performed.Results: The 3D printed handheld electrospinning device successfully fabricates PLA/gelatin nanofibrous membrane with uniformly layered nanofibers and good biocompatibility. Animal experiments showed that the mice in the experimental group had complete skin repair.Conclusions: The 3D printed handheld device can achieve in situ repair of full-thickness defects in mouse skin.

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xinchen Du ◽  
Le Wu ◽  
Hongyu Yan ◽  
Zhuyan Jiang ◽  
Shilin Li ◽  
...  

AbstractDeveloping an anti-infective shape-memory hemostatic sponge able to guide in situ tissue regeneration for noncompressible hemorrhages in civilian and battlefield settings remains a challenge. Here we engineer hemostatic chitosan sponges with highly interconnective microchannels by combining 3D printed microfiber leaching, freeze-drying, and superficial active modification. We demonstrate that the microchannelled alkylated chitosan sponge (MACS) exhibits the capacity for water and blood absorption, as well as rapid shape recovery. We show that compared to clinically used gauze, gelatin sponge, CELOX™, and CELOX™-gauze, the MACS provides higher pro-coagulant and hemostatic capacities in lethally normal and heparinized rat and pig liver perforation wound models. We demonstrate its anti-infective activity against S. aureus and E. coli and its promotion of liver parenchymal cell infiltration, vascularization, and tissue integration in a rat liver defect model. Overall, the MACS demonstrates promising clinical translational potential in treating lethal noncompressible hemorrhage and facilitating wound healing.


Sensors ◽  
2021 ◽  
Vol 21 (15) ◽  
pp. 5032
Author(s):  
Alec Ikei ◽  
James Wissman ◽  
Kaushik Sampath ◽  
Gregory Yesner ◽  
Syed N. Qadri

In the functional 3D-printing field, poly(vinylidene fluoride-co-trifluoroethylene) (PVDF-TrFE) has been shown to be a more promising choice of material over polyvinylidene fluoride (PVDF), due to its ability to be poled to a high level of piezoelectric performance without a large mechanical strain ratio. In this work, a novel presentation of in situ 3D printing and poling of PVDF-TrFE is shown with a d33 performance of up to 18 pC N−1, more than an order of magnitude larger than previously reported in situ poled polymer piezoelectrics. This finding paves the way forward for pressure sensors with much higher sensitivity and accuracy. In addition, the ability of in situ pole sensors to demonstrate different performance levels is shown in a fully 3D-printed five-element sensor array, accelerating and increasing the design space for complex sensing arrays. The in situ poled sample performance was compared to the performance of samples prepared through an ex situ corona poling process.


2021 ◽  
Author(s):  
Zhenzhan Yan ◽  
Li Yang ◽  
Ji-Min Han ◽  
Haojie Li ◽  
Junda Huo

In this work, a nano-scale carbon-based lead azide initiating film was prepared by electrospinning, carbonization, azide, and other steps using cheap and easily available lead acetate as the raw material....


2018 ◽  
Vol 78 (11) ◽  
pp. 2321-2327 ◽  
Author(s):  
Yan Ma ◽  
Zhihuan Zhao ◽  
Jimin Fan ◽  
Zhanyong Gu ◽  
Bin Zhang ◽  
...  

Abstract Using tetra-n-butyl titanate as raw material and fly ash cenospheres (FAC) as carrier, the photocatalysts of Ag-TON/FAC were successfully prepared by solvothermal and in-situ hydrolysis method. These visible light photocatalysts were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), fluorescence spectroscopy (FL) and UV–vis diffuse reflectance spectra (DRS). In this study, methyl orange and ciprofloxacin were used as wastewater degradation targets to investigate the effect of the amount of titanium dioxide and the amount of Ag doping on the activity of photocatalysts. On the basis of this, the optimal ratio of TiO2 to FAC was 2:1 and the optimum doping ratio of Ag was determined to be 15 wt.%. The composite photocatalysts dispersed uniformly and were easy to recycle and reuse, which were benefits in fully utilizing the solar energy. The degradation efficiency remained at more than 60% after being renewed five times for MO and ciprofloxacin. The photocatalysts of Ag-TON/FAC can reduce the environmental burden caused by FAC also.


2017 ◽  
Vol 17 ◽  
pp. 135-142 ◽  
Author(s):  
Oliver Holzmond ◽  
Xiaodong Li

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Marc Sartison ◽  
Simone Luca Portalupi ◽  
Timo Gissibl ◽  
Michael Jetter ◽  
Harald Giessen ◽  
...  

2019 ◽  
Vol 1 (2-2) ◽  
Author(s):  
Mohd Jumain Jalil ◽  
Intan Suhada Azmi ◽  
Arif Aminuddin Ahmad Zulkifli ◽  
Mohamad Helmi Mohd Asbi

The epoxidized vegetables oils can be used a raw material for a broad range of products, from pharmaceutical and plastics to paint and adhesives. Epoxidation of oleic acid was carried out by using hydrogen peroxide as an oxygen donor and formic acid as an oxygen carrier in the presence of sulphuric acid act as catalyst. The crude oleic acid contained 75% oleic acid, 12.2% linoleic acid, 6.5% palmitic acid and 7.5% stearic acid, and had an iodine value of 98.99 g/100 g. The epoxidation of oleic acid with almost complete conversion of unsaturated carbon and negligible oxirane cleavage can be obtained by the in situ techniques. An analytical approach for the prediction of the partition coefficient for formic acid between oleic acid and water, dependent on temperature and composition, has been proposed.


1999 ◽  
Vol 82 (4) ◽  
pp. 825-829 ◽  
Author(s):  
Danica Agbaba ◽  
Tatjana Miljkovic ◽  
Valentina Marinkovic ◽  
Dobrila Zivanov-Stakic ◽  
Sote Vladimirov

Abstract A simple and reliable thin-layer chromatographic method for determining sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate was developed and validated. A methylene chloride–methanol–ammonia solution (25%; 18 + 2.8 + 0.4, v/v) solvent system is used for separation and quantitative evaluation of chromatograms. The chromatographic plate is first scanned at 240 nm to locate chromatographic zones corresponding to sulpiride and methyl-5-sulphamoyl-2-methoxybenzoate. Then 2-aminomethyl-1-ethylpyrrolidine is derivatized in situ with ninhydrin, and resulting colored spots are measured at 500 nm. The method is reproducible and convenient for quantitative analysis and purity control of sulpiride in its raw material and in its dosage forms.


2020 ◽  
Vol 33 ◽  
pp. 101099
Author(s):  
Eduardo Meraz Trejo ◽  
Xavier Jimenez ◽  
Kazi Md Masum Billah ◽  
Jonathan Seppala ◽  
Ryan Wicker ◽  
...  

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