scholarly journals Streptomycin Sulfate–Loaded Niosomes Enables Increased Antimicrobial and Anti-Biofilm Activities

Author(s):  
Maryam Mansouri ◽  
Nazanin Khayam ◽  
Elham Jamshidifar ◽  
Tara Pourseif ◽  
Sepideh Kianian ◽  
...  

One of the antibiotics used to treat infections is streptomycin sulfate that inhibits both Gram-negative and -positive bacteria. Nanoparticles are suitable carriers for the direct delivery and release of drug agents to infected locations. Niosomes are one of the new drug delivery systems that have received much attention today due to their excellent biofilm penetration property and controlled release. In this study, niosomes containing streptomycin sulfate were prepared by using the thin layer hydration method and optimized based on the size, polydispersity index (PDI), and encapsulation efficiency (EE%) characteristics. It was found that the Span 60-to-Tween 60 ratio of 1.5 and the surfactant-to-cholesterol ratio of 1.02 led to an optimum formulation with a minimum of size, low PDI, and maximum of EE of 97.8 nm, 0.27, and 86.7%, respectively. The drug release investigation showed that 50.0 ± 1.2% of streptomycin sulfate was released from the niosome in 24 h and reached 66.4 ± 1.3% by the end of 72 h. Two-month stability studies at 25° and 4°C showed more acceptable stability of samples kept at 4°C. Consequently, antimicrobial and anti-biofilm activities of streptomycin sulfate–loaded niosomes against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were found significantly higher than those of free drug, and the minimum inhibitory concentration values decreased 4- to 8-fold. Furthermore, niosome-encapsulated streptomycin up to 1,500 μg/ml exhibited negligible cytotoxicity against the human foreskin fibroblasts cell line, whereas the free drug exhibited slight cytotoxicity at this concentration. Desired physical characteristics and low toxicity of niosomal nano-carriers containing streptomycin sulfate made them a demanded candidate for the treatment of current bacterial infections and biofilms.

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0247859
Author(s):  
Chiao-Yi Huang ◽  
Wei-Sheng Yu ◽  
Geng-Chia Liu ◽  
Shih-Che Hung ◽  
Jen-Hsiang Chang ◽  
...  

The large amounts of engineered titanium dioxide nanoparticles (TiO2NPs) that have been manufactured have inevitably been released into the ecosystem. Reports have suggested that TiO2 is a relatively inert material that has low toxicity to animals. However, as various types of NPs increasingly accumulate in the ocean, their effects on aquatic life-forms remain unclear. In this study, a zebrafish model was used to investigate TiO2NP-induced injury and mortality. We found that the treatment dosages of TiO2NP are positively associated with increased motility of zebrafish and the bacterial counts in the water. Notably, gill but not dorsal fin and caudal fin of the zebrafish displayed considerably increased bacterial load. Metagenomic analysis further revealed that gut microflora, such as phyla Proteobacteria, Bacteroidetes, and Actinobacteria, involving more than 95% of total bacteria counts in the NP-injured zebrafish gill samples. These results collectively suggest that opportunistic bacterial infections are associated with TiO2NP-induced mortality in zebrafish. Infections secondary to TiO2NP-induced injury could be a neglected factor determining the detrimental effects of TiO2NPs on wild fish.


2015 ◽  
Vol 69 (1-2) ◽  
pp. 80-84
Author(s):  
A. N. Kulichenko ◽  
M. E. Mikhailova ◽  
D. A. Kovalev ◽  
S. V. Pisarenko ◽  
U. V. Siriza ◽  
...  

Aim: to study features of pharmacokinetics of ofloxacin as a part of anion PEGylated niosomes on a basis of sorbitan monostearate (Span 60) to experimental white mice per os. Materials and methods: ofloxacin was entrapped in niosomes consisting of Span 60, cholesterol, PEG 4000 and dicetylphosphate. Sizes of niosomes estimated by means of probe microscopy. Efficiency of inclusion of an antibiotic in niosomes defined after removal of free drug by a centrifugation. The analysis of the quantitative contents of ofloxacin in samples carried out a method of a high performance liquid chromatography. Results: we studied the main pharmacokinetic parameters of ofloxacin when used free and niosomal forms of antibiotic to experimental white mice per os. It is shown that use of oral niosomal forms leads to decrease of maximal concentration in serum and increase of ofloxacin half-life by 7,4 times in average compared to the free form. It is determined that bioavailability of ofloxacin in the niosomal form is 154% relative to the free form of the antibiotic. Conclusions: niosomal microcontainers are perspective technology of encapsulation and the directed transport of antibacterial preparations through biological barriers. Using of niosomal formulation of ofloxacin is able to afford to increase considerably efficiency of treatment in comparison with a free form and significantly decrease negative effects of antibiotic therapy.


Media Farmasi ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 1
Author(s):  
Arisanty Arisanty ◽  
Santi Sinala ◽  
Muli Sukmawaty ◽  
Andi Masna

Gotu Kola (Centella asiatica (L.) Urb) is a plant widely grown and scientifically proven to be an antioxidant. This study makes a lotion formula containing dried Gotu kola herb with varying concentrations of emulgator span 60 and tween 60. It determines the concentration of span 60 and tween 60, producing lotions with the most stable physical quality and accelerated storage methods. The lotion was prepared using a concentration of Centellaasiatica (L.) Herbaceous Dry Herb extract of 1% and an Emulgator span 60 and tween 60 in a concentration variation of 5%, 7.5%, and 10%. The stability of the lotion was determined based on quality observations. The physical condition before and after the storage is accelerated for six cycles at a temperature of 5oC and 35oC in organoleptic, homogeneous, pH, viscosity, diffusion power, and emulsion type. The research results show the physical quality stability of formula I (5% emulgator) did not meet the requirements of the dispersion test before storage was accelerated. In contrast, the formula with a 7.5% emulgator met the physical quality requirements of the preparation before and after storage was accelerated. Additionally, the 10% emulgator did not meet the requirements of the dispersion test after accelerated storage. The type of emulsion in all three formulas is the M / A type. The most stable physical quality of the three preparations is a formula with a 7.5% emulgator.Keywords: Lotion, Gotu kola (Centella asiatica (L.) Urb), physical quality, span, and tween.Pegagan (Centella asiatica(L.)Urb) merupakan tanaman yang banyak tumbuh di Indonesia dan telah terbukti secara ilmiah memiliki kemampuan sebagai antioksidan. Penelitian ini bertujuan  untuk membuat formula lotion yang  mengandung sari kering herba pegagan (Centella asiatica(L.) Urban) dengan variasi konsentrasi emulgator span 60 dan tween 60, dan untuk mengetahui konsetrasi span 60 dan tween 60 yang menghasilkan lotion dengan mutu fisik yang paling stabil dengan metode penyimpanan dipercepat. Sediaan dibuat lotion dengan konsentrasi sari Kering Herba  Pegagan (Centellaasiatica(L.) Urban ) sebesar 1% dan Emulgator span 60 serta tween 60 dengan variasi konsentrasi 5%, 7,5%, dan 10% kemudian stabilitas sedian lotion ditentukan berdasarkan pengamatan mutu fisik sebelum dans sesudah penyimpanan dipercepat selama 6 siklus pada suhu 5oCdan 35oC yang meliputi organeleptis, homogentias, pH, viskositas, daya sebar, dan tipe emulsi. Hasil penelitian pada pengujian kestabilan mutu fisik formula I (emulgator 5%) tidak memenuhi syarat pada uji daya sebar sebelum penyimpanan dipercepat, sedangkan Formula dengan emulgator 7,5% memenuhi persyaratan mutu fisik sediaan sebelum maupun sesudah penyimpanan dipercepat, dan formula dengan emulgator 10% tidak memenuhi syarat pada uji daya sebar setelah penyimpanan dipercepat, untuk tipe emulsi pada ketiga formula tersebut merupakan tipe emulsiM/A. Mutu fisik yang paling stabil dari ketiga sediaan tersebut adalah formula dengan emulgator 7,5%.Kata kunci : Lotion,  herba pegagan (Centella asiatica (L.) Urb), mutu fisik, span dan tween.


2020 ◽  
Vol 6 (1) ◽  
pp. 21-26
Author(s):  
Kartika Zulfa ◽  
◽  
Ferri Widodo ◽  
Oktavia Eka Puspita ◽  
◽  
...  

Excessive radiation from UV light can cause skin damage to melanoma, especially UVB rays. Chronic effects from exposure of excessive UVB rays can induce gene mutations because the exposure of excessive UVB rays directly causes damage to cellular DNA by producing ROS in the epidermis, dermis, and skin epithelium cells. The use of sunscreen is very necessary to prevent skin damage. Sunscreen containing antioxidants are highly recommended to protect the skin from free radicals UVB rays. Pterostilbene is one of the phenolic compounds, which has the pharmacological activity of antioxidants and UV filters to be one of the recommended compounds for sunscreen components. A good delivery system is needed to be formulated to improve the pharmacological effects of pterostilbene on topical use. Niosomes are non-ionic surfactant vesicles which are one of the amphiphilic carrier systems which can carry hydrophobic active ingredients such as pterostilbene, which are expected to increase the pharmacological effect by increasing the penetration of pterostilbene into the skin. Pterostilbene niosome using non-ionic surfactant (span 80 and span 60) by thin layer hydration method. The research aimed to examine the effect of surfactant concentration (span 80 and span 60) 2, 4, and 6 g toward the characterization of niosome pterostilbene and determine the optimum formulation by particle size. The results of the study showed that the particle size was smaller with an increase in span concentration. Based on these results, the optimum formulation of pterostilbene niosomes is obtained using span 60 with a concentration of 6 g.


2020 ◽  
Vol 10 (1) ◽  
pp. 54-60
Author(s):  
Rashmi Sareen ◽  
Nitin Jain

Objective: The purpose of the present study was to develop a novel elastic bilayer vesicle entrapped with Flurbiprofen (FLB) for transdermal use to avoid adverse effect associated with oral administration of the drug. Encapsulation of drug in vesicle prolongs the existence of the drug in the systemic circulation and thus enhances penetration into the target site and reduces toxicity. Method: Niosomes were prepared using surfactants (span 40 and span 60) and cholesterol in the molar ratio of 1:1, 2:1, 3:1 and 3:2. Vesicles prepared by thin film hydration method were characterized for morphology, vesicle size and zeta potential, thermal analysis and Entrapment Efficiency (EE). Results: Results revealed that the EE and size of niosomes were influenced by surfactant type and cholesterol ratio. F8 (span 60: cholesterol in 3:2) exhibited the highest encapsulation of FLB (76.77 ± 0.55) with vesicle size of 154 ± 2.96 nm and Polydispersity Index (PDI) of 0.09. The optimized formulation F8 was selected for incorporation into the gel. Niosomal gel was evaluated for homogeneity, pH, spreadability and in-vitro drug release. Conclusion: All the parameters of niosomal gel were found to be satisfactory and in-vitro release study revealed prolonged and complete release of entrapped FLB (93.23±0.65%) in comparison to FLB hydrogel (42.65±0.29%). The results suggested that niosomes may serve as promising vehicles for the transdermal delivery of FLB.


Author(s):  
Krystyna Dąbrowska ◽  
Stephen T. Abedon

SUMMARY The use of viruses infecting bacteria (bacteriophages or phages) to treat bacterial infections has been ongoing clinically for approximately 100 years. Despite that long history, the growing international crisis of resistance to standard antibiotics, abundant anecdotal evidence of efficacy, and one successful modern clinical trial of efficacy, this phage therapy is not yet a mainstream approach in medicine. One explanation for why phage therapy has not been subject to more widespread implementation is that phage therapy research, both preclinical and clinical, can be insufficiently pharmacologically aware. Consequently, here we consider the pharmacological obstacles to phage therapy effectiveness, with phages in phage therapy explicitly being considered to serve as drug equivalents. The study of pharmacology has traditionally been differentiated into pharmacokinetic and pharmacodynamic aspects. We therefore separately consider the difficulties that phages as virions can have in traveling through body compartments toward reaching their target bacteria (pharmacokinetics) and the difficulties that phages can have in exerting antibacterial activity once they have reached those bacteria (pharmacodynamics). The latter difficulties, at least in part, are functions of phage host range and bacterial resistance to phages. Given the apparently low toxicity of phages and the minimal side effects of phage therapy as practiced, phage therapy should be successful so long as phages can reach the targeted bacteria in sufficiently high numbers, adsorb, and then kill those bacteria. Greater awareness of what obstacles to this success generally or specifically can exist, as documented in this review, should aid in the further development of phage therapy toward wider use.


2007 ◽  
Vol 57 (1) ◽  
pp. 61-62 ◽  
Author(s):  
Ana Giménez-Arnau ◽  
Montserrat Gilaberte ◽  
David Conde ◽  
Merce Espona ◽  
Ramon M. Pujol
Keyword(s):  
Tween 60 ◽  

Author(s):  
ANISA AMALIA ◽  
YUDI SRIFIANA ◽  
AMALIA ANWAR

Objective: Curcumin penetration can be increased by formulating it into the transethosome system. Surfactant is one of the transethosome components that affect the physical properties and penetration of vesicles. In this study, a combination of two surfactants was used to see the effect of surfactants on physical properties and curcumin penetration. Methods: This study used a combination of tween 60 and span 60 with a concentration ratio of 0:5 (F1), 1:1 (F2), 2:1 (F3), and 1:2 (F4). An evaluation included testing the distribution of particle size, zeta potential, and entrapment efficiency in the system. Evaluation continued with the determination of the diffusion rate in vitro.  Results: The transethosome system formed has a particle size of 167.9±4.7 nm-396±3.7 nm with a potential zeta value (-) 49.54±1.77 mV-(-) 59.05±0.95 mV, polydispersion index 0.0%-57.1% and entrapment efficiency of 83.76%-93.75%. The diffusion rate of F1 and F3 followed the Higuchi kinetics model, while F2 and F4 followed zero-order kinetics and the Korsmeyer-Peppas kinetics. Conclusion: The combination of tween 60 and span 60 could form a nano-sized transethosome of curcumin. Diffusion rate testing results show that using a surfactant combination can increase the diffusion rate of curcumin, where there is a significant difference between each formula (p<0.05).


Sign in / Sign up

Export Citation Format

Share Document