scholarly journals Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth

2021 ◽  
Vol 9 ◽  
Author(s):  
Yufan Zhang ◽  
Junfei Huang ◽  
Danlan Fu ◽  
Zhen Liu ◽  
Hailin Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
3D Model ◽  
Caspase 3 ◽  
Dermal Papilla ◽  
Inhibitory Effect ◽  
Follicle Growth ◽  
Hub Genes ◽  
Dermal Papilla Cells ◽  
Hair Follicle Growth ◽  
2D And 3D

Dermal papillae are a target of androgen action in patients with androgenic alopecia, where androgen acts on the epidermis of hair follicles in a paracrine manner. To mimic the complexity of the dermal papilla microenvironment, a better culture model of human dermal papilla cells (DPCs) is needed. Therefore, we evaluated the inhibitory effect of dihydrotestosterone (DHT)-treated two-dimensional (2D)- and 3D-cultured DPCs on hair follicle growth. 2D- and 3D-cultured DPC proliferation was inhibited after co-culturing with outer root sheath (ORS) cells under DHT treatment. Moreover, gene expression levels of β-catenin and neural cell adhesion molecules were significantly decreased and those of cleaved caspase-3 significantly increased in 2D- and 3D-cultured DPCs with increasing DHT concentrations. ORS cell proliferation also significantly increased after co-culturing in the control-3D model compared with the control-2D model. Ki67 downregulation and cleaved caspase-3 upregulation in DHT-treated 2D and 3D groups significantly inhibited ORS cell proliferation. Sequencing showed an increase in the expression of genes related to extracellular matrix synthesis in the 3D model group. Additionally, the top 10 hub genes were identified, and the expression of nine chemokine-related genes in DHT-treated DPCs was found to be significantly increased. We also identified the interactions between transcription factor (TF) genes and microRNAs (miRNAs) with hub genes and the TF–miRNA coregulatory network. Overall, the findings indicate that 3D-cultured DPCs are more representative of in vivo conditions than 2D-cultured DPCs and contribute to our understanding of the molecular mechanisms underlying androgen-induced alopecia.

scholarly journals Platelet factor 4 inhibits human hair follicle growth and promotes androgen receptor expression in human dermal papilla cells

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9867
Author(s):  
Ke Sha ◽  
Mengting Chen ◽  
Fangfen Liu ◽  
San Xu ◽  
Ben Wang ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Hair Follicle ◽  
Human Hair ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Follicle Growth ◽  
Human Hair Follicle ◽  
Dermal Papilla Cells ◽  
Platelet Factor ◽  
Hair Follicle Growth

Platelet-rich plasma (PRP) has been reported recently as a potential therapeutic approach for alopecia, such as androgenetic alopecia, but the exact mechanisms and effects of specific components of this recipe remain largely unknown. In this study, we identified that platelet factor 4 (PF4), a component of PRP, significantly suppressed human hair follicle growth and restrained the proliferation of human dermal papilla cells (hDPCs). Furthermore, our results showed that PF4 upregulated androgen receptor (AR) in human dermal papilla cells in vitro and via hair follicle organ culture. Among the hair growth-promoting and DP-signature genes investigated, PF4 decreased the expression of Wnt5a, Wnt10b, LEF1, HEY1 and IGF-1, and increased DKK1 expression, but did not affect BMP2 and BMP4 expression. Collectively, Our data demonstrate that PF4 suppresses human hair follicle growth possibly via upregulating androgen receptor signaling and modulating hair growth-associated genes, which provides thought-provoking insights into the application and optimization of PRP in treating hair loss.

scholarly journals Regulatory role of LEF-1 in the proliferation of Arbas White Cashmere goat dermal papilla cells

2018 ◽  
Vol 98 (4) ◽  
pp. 667-674
Author(s):  
Fei Hao ◽  
Wei Yan ◽  
Xiaodong Guo ◽  
Bing Zhu ◽  
Dongjun Liu
Keyword(s):  
Wnt Signaling ◽  
Cyclin D1 ◽  
Economic Value ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Cashmere Goat ◽  
Follicle Growth ◽  
Dermal Papilla Cells ◽  
Hair Follicle Growth

Cashmere, which has high economic value, is made from the secondary hair follicles of cashmere goat skin. Dermal papilla cells (DPCs) are considered the center for regulation of hair growth, which is closely related to hair follicle growth. We constructed LEF-1 overexpression and interference experimental groups of goat DPCs to investigate LEF-1 regulation of DPCs proliferation by Wnt signaling, and provide a theoretical basis for improving cashmere yield. In primary DPCs, LEF-1, β-catenin, C-myc, and cyclin D1 expression in the LEF-1 overexpression group was 9.25-, 1.27-, 1.74-, and 1.63-fold, respectively, that of the control. LEF-1, β-catenin, C-myc, and cyclin D1 expression in the LEF-1 interference group was 0.20-, 0.75-, 0.38-, and 0.39-fold, respectively, that of the control. In secondary DPCs, LEF-1, β-catenin, C-myc, and cyclin D1 expression in the LEF-1 overexpression group was 10.53-, 1.48-, 1.64-, and 1.39-fold, respectively, that of the control. LEF-1, β-catenin, C-myc, and cyclin D1 expression in the LEF-1 interference group was 0.21-, 0.71-, 0.40-, and 0.36-fold, respectively, that of the control. Primary and secondary DPCs proliferation rates changed with LEF-1 expression. Therefore, the LEF-1 regulation pattern of cell proliferation through Wnt signaling is similar in both DPCs.

scholarly journals Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods

2020 ◽  
Vol 33 (5) ◽  
pp. 280-292
Author(s):  
Ehsan Taghiabadi ◽  
Mohammad Ali Nilforoushzadeh ◽  
Nasser Aghdami
Keyword(s):  
Hair Follicle ◽  
Dermal Papilla ◽  
Culture Conditions ◽  
Hair Follicles ◽  
Main Role ◽  
Follicle Growth ◽  
In Vitro Morphogenesis ◽  
Dermal Papilla Cells ◽  
Hair Follicle Growth

The dermal papilla comprises mesenchymal cells in hair follicles, which play the main role in regulating hair growth. Maintaining the potential hair inductivity of dermal papilla cells (DPCs) and dermal sheath cells during cell culture is the main factor in in vitro morphogenesis and regeneration of hair follicles. Using common methods for the cultivation of human dermal papilla reduces the maintenance requirements of the inductive capacity of the dermal papilla and the expression of specific dermal papilla biomarkers. Optimizing culture conditions is therefore crucial for DPCs. Moreover, exosomes appear to play a key role in regulating the hair follicle growth through a paracrine mechanism and provide a functional method for treating hair loss. The present review investigated the biology of DPCs, the molecular and cell signaling mechanisms contributing to hair follicle growth in humans, the properties of the dermal papilla, and the effective techniques in maintaining hair inductivity in DPC cultures in humans as well as hair follicle bioengineering.

Boehmite enhances hair follicle growth via stimulation of dermal papilla cells by upregulating β‐catenin signalling

2019 ◽  
Vol 29 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Dong‐Ho Bak ◽  
Esther Lee ◽  
Byung‐chul Lee ◽  
Mi Ji Choi ◽  
Tae‐Rin Kwon ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Dermal Papilla ◽  
Follicle Growth ◽  
Dermal Papilla Cells ◽  
Hair Follicle Growth ◽  
Stimulation Of
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