scholarly journals Hypochlorite-Activated Fluorescence Emission and Antibacterial Activities of Imidazole Derivatives for Biological Applications

2021 ◽  
Vol 9 ◽  
Author(s):  
Thanh Chung Pham ◽  
Van-Nghia Nguyen ◽  
Yeonghwan Choi ◽  
Dongwon Kim ◽  
Ok-Sang Jung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Stokes Shift ◽  
Fluorescence Emission ◽  
Antibacterial Activities ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Fluorogenic Probe ◽  
High Fluorescence

The ability to detect hypochlorite (HOCl/ClO−) in vivo is of great importance to identify and visualize infection. Here, we report the use of imidazoline-2-thione (R1SR2) probes, which act to both sense ClO− and kill bacteria. The N2C=S moieties can recognize ClO− among various typical reactive oxygen species (ROS) and turn into imidazolium moieties (R1IR2) via desulfurization. This was observed through UV–vis absorption and fluorescence emission spectroscopy, with a high fluorescence emission quantum yield (ՓF = 43–99%) and large Stokes shift (∆v∼115 nm). Furthermore, the DIM probe, which was prepared by treating the DSM probe with ClO−, also displayed antibacterial efficacy toward not only Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) but also methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum ß-lactamase–producing Escherichia coli (ESBL-EC), that is, antibiotic-resistant bacteria. These results suggest that the DSM probe has great potential to carry out the dual roles of a fluorogenic probe and killer of bacteria.

scholarly journals The Study of Antibacterial Properties of Anbarnasara Smoke on Multi-Drug Resistant Bacteria Isolated From Urinary Infection in Pregnant Women

2020 ◽  
Vol 8 (4) ◽  
pp. 110-115
Author(s):  
Afsaneh Molamirzaei ◽  
Maryam Allahdadian ◽  
Monir Doudi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Pregnant Women ◽  
Urinary Infection ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Drug Resistant

Background: Using smoke from burning donkey dung has been popular in the treatment of many diseases in Iran. Objective: This study aimed to investigating the antimicrobial properties of donkey dung smoke on multi-drug resistant (MDR) bacteria isolated from urinary infection. Materials and Methods: First, 300 and 200 urine samples were collected from pregnant and non-pregnant women in Isfahan, Iran. Then in each group, 100 bacterial isolates including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus saprophyticus were isolated. Antibiotic resistant protocol was determined by antibiogram test. Donkey dung was sterilized, disintegrated, and heated. The smokes were concentrated in n-hexane solvent (65%) and were collected after evaporation of the solvent. Finally, the antibacterial activities of the concentrations of 0.25, 0.5 and 1 mg/mL of the smokes were detected using disk diffusion and macrodilution methods. Results: The most abundant MDR isolates causing urinary infections in pregnant and non-pregnant women was Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of donkey dung smoke on MDR isolates from pregnant women were 0.25 mg/mL and 0.5 mg/mL, respectively. In the case of MDR isolates in non-pregnant women, the MIC of the smoke on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was 0.25 mg/mL, and the MBC on these isolates was 0.5 mg/mL. Conclusion: The smokes from donkey dung investigated in the present study have suitable potentials for controlling the infections after In vivo analysis.

Antibacterial Effects of Derivatives of Porphyrin, Naphthalene diimide, Aminophenol and Benzodioxane on Methicillin Resistant Staphylococcus aureus and Neuropathogenic Escherichia coli K1

2020 ◽  
Vol 18 (3) ◽  
pp. 275-284
Author(s):  
Ruqaiyyah Siddiqui ◽  
Ayaz Anwar ◽  
Salwa Ali ◽  
Naveed Ahmed Khan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Host Cells ◽  
Mortality And Morbidity ◽  
Naphthalene Diimide ◽  
Molecular Mode ◽  
Escherichia Coli K1

Background: Infectious diseases contribute to substantial mortality and morbidity worldwide despite advances in therapeutic intervention highlighting the need to identify drugs with antimicrobial properties. Methods: Here, we utilised several compounds from the following classes: porphyrin, naphthalene diimide, aminophenol derivatives, and benzodioxane, and evaluated their antibacterial activities. Bactericidal and bacteriostatic activity of these compounds were determined against methicillinresistant Staphylococcus aureus (MRSA) and Escherichia coli K1 with various concentrations of the drugs. Moreover, the ability of the bacteria to bind/associate to host cells was also ascertained in the absence and presence of aforementioned compounds. Results: The results revealed that porphyrin derivative (AYTHPP) had potent effects against MRSA, abolishing viability and blocking binding to the host cells. Importantly, novel AYTHPP exhibited powerful effects against MRSA even though it was not photoactivated. In contrast, other compounds, including naphthalene diimide, acetamol derivatives and benzodioxane, showed no inhibitory effects. Conclusion: The mechanism of action of porphyrin is likely through the production of reactive oxygen species causing oxidative stress, leading to apoptosis and/or necrosis via perturbations in the plasma membrane. Future studies will determine their in vivo efficacy together will associated molecular mode of action.

scholarly journals Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

2020 ◽  
pp. 795
Author(s):  
Rania Benjamaa ◽  
Abdelkarim Moujanni ◽  
Anass Terrab ◽  
Rabiaa Eddoha ◽  
Maryam Benbachir ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Antibiotic Residues ◽  
Antibiotic Resistant ◽  
Liquid Chromatography Tandem Mass

Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence InvestigatorTM, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal.

scholarly journals In Vivo Antibacterial Activities of Sanfetrinem Cilexetil, a New Oral Tricyclic Antibiotic

1998 ◽  
Vol 42 (7) ◽  
pp. 1858-1861 ◽  
Author(s):  
Shinobu Tamura ◽  
Shuichi Miyazaki ◽  
Kazuhiro Tateda ◽  
Akira Ohno ◽  
Yoshikazu Ishii ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pyogenes ◽  
Respiratory Infections ◽  
Antibacterial Activities ◽  
Murine Models ◽  
In Vitro Activity ◽  
Number Of Bacteria

ABSTRACT The in vivo antibacterial activities of a new oral trinem, sanfetrinem cilexetil (a prodrug of sanfetrinem), were evaluated in comparison with those of cefdinir and amoxicillin. Sanfetrinem cilexetil showed potent efficacy against experimental murine septicemia caused by Staphylococcus aureus, Streptococcus pyogenes, and Escherichia coli and against murine respiratory infections caused by Streptococcus pneumoniae. Likewise, in murine models of respiratory infection by penicillin-susceptible and penicillin-resistant S. pneumoniae, sanfetrinem cilexetil was more effective than amoxicillin in reducing the number of bacteria in infected lungs. These results were reflected in its potent in vitro activity and high levels in plasma.

Antibacterial Activities of Naturally Occurring Compounds against Antibiotic-Resistant Bacillus cereus Vegetative Cells and Spores, Escherichia coli, and Staphylococcus aureus

2004 ◽  
Vol 67 (8) ◽  
pp. 1774-1778 ◽  
Author(s):  
MENDEL FRIEDMAN ◽  
ROBERT BUICK ◽  
CHRISTOPHER T. ELLIOTT
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Resistant Bacteria ◽  
Vegetative Cells ◽  
Cinnamon Oil ◽  
Antibiotic Resistant ◽  
Thyme Oil ◽  
Oregano Oil ◽  
And Staphylococcus Aureus

After demonstrating the lack of effectiveness of standard antibiotics against the acquired antibiotic resistance of Bacillus cereus (NCTC 10989), Escherichia coli (NCTC 1186), and Staphylococcus aureus (ATCC 12715), we showed that the following natural substances were antibacterial against these resistant pathogens: cinnamon oil, oregano oil, thyme oil, carvacrol, (S)-perillaldehyde, 3,4-dihydroxybenzoic acid (β-resorcylic acid), and 3,4-dihydroxyphenethylamine (dopamine). Exposure of the three pathogens to a dilution series of the test compounds showed that oregano oil was the most active substance. The oils and pure compounds exhibited exceptional activity against B. cereus vegetative cells, with oregano oil being active at nanogram per milliliter levels. In contrast, activities against B. cereus spores were very low. Activities of the test compounds were in the following approximate order: oregano oil > thyme oil ≈ carvacrol > cinnamon oil > perillaldehyde > dopamine>β-resorcylic acid. The order of susceptibilities of the pathogens to inactivation was as follows: B. cereus (vegetative) ≫S. aureus ≈ E. coli ≫ B. cereus (spores). Some of the test substances may be effective against antibiotic-resistant bacteria in foods and feeds.

scholarly journals Incidence and antibiotic susceptibility pattern of Escherichia coli and Staphylococcus aureus isolated from meat pie sold in a Nigerian North Central town

2018 ◽  
Vol 6 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Godwin Attah Obande ◽  
Ebele U. Umeh ◽  
Emmanuel Terese Azua ◽  
Aleruchi Chuku ◽  
Peter Adikwu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Contamination ◽  
Resistant Bacteria ◽  
Disc Diffusion ◽  
Antibiotic Resistant ◽  
North Central ◽  
Antibiotic Susceptibility Pattern ◽  
Bacillus Spp ◽  
And Staphylococcus Aureus

Background and Objectives: Meat pie is a popular ready-to-eat food sold in Nigeria and is consumed by people of all classes and category. The study aimed to determine the incidence and susceptibility of Escherichia coli and Staphylococcus aureus isolated from meat pie to antibiotics commonly administered in Makurdi.Material and Methods: A total of 180 samples were collected and evaluated for bacterial contamination and presence of antibiotic-resistant Escherichia coli and Staphylococcus aureus. Contaminants were isolated and identified using biochemical test. Antimicrobial susceptibility of isolates was determined using the Kirby-Bauer disc diffusion method.Results: Eleven bacterial genera was identified.  Bacillus spp (85%) occurred most frequently, followed by Staphylococcus aureus (38.9%), while Edwardsiella spp (2.8%) occured the least.  Staphylococcus aureus was highly resistant to Cloxacillin (87.1%) but highly susceptible to Ofloxacin (88.6%).  Escherichia coli was resistant to Amoxycillin, Tetracycline, Cloxacillin and Augmentin but susceptible to Gentamicin and Ofloxacin. Conclusion: Meat pie sold in Makurdi habours Staphylococcus aureus and Escherichia coli with multiple antibiotic resistance. Regulation of the production and retail process of meat pie is advocated as a possible means of reducing contamination and the risk of transferring antibiotic resistant bacteria to consumers.

scholarly journals In Vitro Activity of Rifabutin and Rifampin against Antibiotic-Resistant Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae

mSphere ◽  
2021 ◽  
Author(s):  
Bosul Lee ◽  
Jun Yan ◽  
Amber Ulhaq ◽  
Sarah Miller ◽  
Wonjae Seo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
General Effect ◽  
In Vitro Activity ◽  
Antibiotic Resistant ◽  
Content Type

Rifabutin has been recently described as a potential adjunctive therapy for antibiotic-resistant A. baumannii infections due to hypersensitivity in iron-depleted media, which may more closely mimic an in vivo environment. Here, we report that this hyperactivity is specific for A. baumannii , rather than being a general effect for other pathogens.

scholarly journals Comparison of transcriptional responses and metabolic alterations in three multidrug resistant model microorganisms, Staphylococcus aureus ATCC BAA-39, Escherichia coli ATCC BAA-196 and Acinetobacter baumannii ATCC BAA-1790, on exposure to iodine-containing nano-micelle drug FS-1

2020 ◽  
Author(s):  
Ilya S. Korotetskiy ◽  
Sergey V. Shilov ◽  
Tatyana V. Kuznetsova ◽  
Aleksandr I. Ilin ◽  
Monique Joubert ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Gene Regulation ◽  
Acinetobacter Baumannii ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Genome Sequences ◽  
Antibiotic Resistant ◽  
Reference Strains ◽  
Sublethal Concentrations

AbstractIodine is one of the oldest antimicrobial agents. Till now there have been no reports on acquiring resistance to iodine. Recent studies showed promising results on application of iodine-containing nano-micelles, FS-1, against antibiotic resistant pathogens as a supplement to antibiotic therapy. The mechanisms of the action, however, remain unclear. The aim of this study was to perform a holistic analysis and comparison of gene regulation in three phylogenetically distant multidrug resistant reference strains representing pathogens associated with nosocomial infections from the ATCC culture collection: Escherichia coli BAA-196, Staphylococcus aureus BAA-39 and Acinetobacter baumannii BAA-1790. These cultures were treated by a 5 min exposure to sublethal concentrations of the iodine-containing drug FS-1 applied in the late lagging and the mid of the logarithmic growth phases. Complete genome sequences of these strains were obtained in the previous studies. Gene regulation was studied by total RNA extraction and Ion Torrent sequencing followed by mapping the RNA reads against the reference genome sequences and statistical processing of read counts using the DESeq2 algorithm. It was found that the treatment of bacteria with FS-1 profoundly affected the expression of many genes involved in central metabolic pathways; however, alterations of the gene expression profiles were species-specific and depended on the growth phase. Disruption of respiratory electron-transfer membrane complexes, increased penetrability of bacterial cell walls, osmotic and oxidative stresses leading to DNA damaging were the major factors influencing the treated bacteria.IMPORTANCEInfections caused by antibiotic resistant bacteria threaten the public health worldwide. Combinatorial therapy when antibiotics are administrated together with supplementary drugs improving susceptibility of pathogens to the regular antibiotics is considered as a promising way to overcome this problem. An induction of antibiotic resistance reversion by the iodine-containing nano-micelle drug FS-1 has been reported recently. This drug is currently under clinical trials in Kazakhstan against multidrug resistant tuberculosis. The effects of released iodine on metabolic and regulatory processes in bacterial cells remain unexplored. The current work provides an insight into gene regulation in the antibiotic resistant nosocomial reference strains treated with iodine-containing nanoparticles. This study sheds light on unexplored bioactivities of iodine and the mechanisms of its antibacterial effect when applied in sublethal concentrations. This knowledge will aid in the future design of new drugs against antibiotic resistant infections.

Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 61-68
Author(s):  
Agnina Listya Anggraini ◽  
Ratih Dewi Dwiyanti ◽  
Anny Thuraidah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Allium Sativum ◽  
Antibacterial Properties ◽  
Herbal Medicines ◽  
Garlic Extract ◽  
Dilution Method ◽  
Initial Stage

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

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