scholarly journals Microbiota in Gingival Crevicular Fluid Before and After Mechanical Debridement With Antimicrobial Photodynamic Therapy in Peri-Implantitis

2022 ◽  
Vol 11 ◽  
Author(s):  
Haiyan Wang ◽  
Ye Liu ◽  
Wei Li ◽  
Wenyue Li ◽  
Hongtao Xu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Treatment Group ◽  
Gingival Crevicular Fluid ◽  
Reference Database ◽  
Antimicrobial Photodynamic Therapy ◽  
Significant Difference ◽  
Before And After ◽  
Pre Treatment ◽  
Crevicular Fluid ◽  
Level 2

ObjectivesThis study aims to compare the microbiota of gingival crevicular fluid (GCF) before and after mechanical debridement (MD) with antimicrobial photodynamic therapy (aPDT) and determine the core efficient microbiota in peri-implantitis after treatment.MethodsWe recruited 9 patients (14 implants) treated with MD+aPDT for peri-implantitis at our center from February 1, 2018, to February 1, 2019. GCF was collected using filter paper strip before and after the treatment. The bacterial 16S rRNA was amplified and sequenced using an Illumina MiSeq platform to characterize the GCF. Bioinformatics and statistical analyses were performed using QIIME2 and R.ResultsA total of 4,110,861 high-quality sequences were obtained from GCF samples. Based on the reference database, 1,120 amplicon sequence variants (ASVs) were finally harvested. Principal coordinates analysis indicated significant differences in the bacterial community structure between the 180 days after-treatment group and pre-treatment group. Difference analysis and least discriminant analysis showed that the differences were mainly reflected in non-dominant bacteria between these two groups. The non-dominant genera with significantly different distribution between the 180 days after-treatment group and the pre-treatment group included Lactobacillus, Pedobacter, Bulleidia, Centipeda, Desulfovibrio, Ochrobactrum, Staphylococcus, Microbacterium, Brevundimonas, Desulfobulbus, and Parvimonas. Moreover, a total of 29 predictive functional categories at KEGG level 2 were identified. The significant difference pathways at KEGG level 2 between after-treatment and pre-treatment were concentrated in infectious disease-related pathways.ConclusionsPatients with peri-implantitis have significant changes in the low-abundance bacteria of the GCF before and after MD+aPDT. MD+aPDT may change the composition of GCF microbiota by increasing the abundance of cluster 1 (beneficial) and decreasing that of cluster 4 (harmful), which may decrease metabolic response to infection and thus improve peri-implantitis.

scholarly journals β-Carotene patch application effects on gingival crevicular fluid volume after panoramic radiography exposure

2020 ◽  
Vol 5 (3) ◽  
pp. 101
Author(s):  
Anggun Dwi Andini ◽  
Rini Widyaningrum ◽  
Rurie Ratna Shantiningsih
Keyword(s):  
Treatment Group ◽  
Gingival Crevicular Fluid ◽  
Panoramic Radiography ◽  
T Test ◽  
Maxillary Incisor ◽  
Patch Application ◽  
Significant Difference ◽  
Before And After ◽  
Crevicular Fluid ◽  
Β Carotene

Panoramic radiography is a radiography technique which is commonly used in dentistry. X-ray radiation from panoramic radiography can cause biological damage, such as the increase of gingival crevicular fluid (GCF) volume. One of materials which was known having the ability to decrease the effect of panoramic radiography is β-carotene in mucoadhesive gingival patch form. The application of β-carotene mucoadhesive gingival patch reduce micronucleus increasing in previous study. The aim of this study is to determine the effect of β-carotene mucoadhesive gingival patch application on palatal gingiva of maxillary incisor teeth to the GCF volume of panoramic radiography exposed patients. This study involved 10 subjects (5 females and 5 males). The GCF sample was taken from the labial sulcus of 11, 12 teeth (treatment group) and 21, 22 teeth (control group) of each subject before and after panoramic radiography exposure. β-carotene mucoadhesive gingival patch was applicated on palatal gingiva before panoramic radiography examination. Sample was collected using an absorbent paper which is inserted to the labial gingival sulcus. The GCF on the absorbent papers were marked by adding ninhydrin 2%, then the GCF volume was measured using a sliding caliper. Paired t-test result shows a significant difference between GCF volume in each group before and after panoramic radiography exposure (p<0.05). Independent t-test shows no significant difference between the GCF volume difference in the control and treatment group (p>0.05). The result of this study shows that the application of β-carotene mucoadhesive gingival patch on palatal gingiva of maxillary incisor teeth shows insignificant effect in decreasing the increase of GCF volume in panoramic radiography exposed patients.

Effects of Antimicrobial Photodynamic Therapy on Transforming Growth Factor-β1 Levels in the Gingival Crevicular Fluid

2013 ◽  
Vol 31 (2) ◽  
pp. 65-71 ◽  
Author(s):  
Sérgio L. S. Souza ◽  
Patrícia F. Andrade ◽  
João S. Silva ◽  
Fabrine S. M. Tristão ◽  
Fernanda A. Rocha ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Gingival Crevicular Fluid ◽  
Transforming Growth Factor Β1 ◽  
Antimicrobial Photodynamic Therapy ◽  
Crevicular Fluid

scholarly journals Effect of β-carotene patch application on gingival crevicular fluid volume after repeated periapical radiographic exposure

2019 ◽  
Vol 4 (3) ◽  
pp. 127
Author(s):  
Faluthia Arini Puspitaningrum ◽  
Rurie Ratna Shantiningsih ◽  
Ryna Dwi Yanuaryska
Keyword(s):  
Treatment Group ◽  
Gingival Crevicular Fluid ◽  
Biological Tissues ◽  
T Test ◽  
Paper Strip ◽  
X Rays ◽  
Patch Application ◽  
Before And After ◽  
Crevicular Fluid ◽  
Β Carotene

Free radicals generated during ionization process of X-rays can damage biological tissues. Radiation exposure to gingival sulcus area will damage endothelial cells and increase permeability of blood vessels under sulcular and junctional epithelium. That inflammation will increase gingival crevicular fluid (GCF) volume. Repeated periapical radiographs often occurs due to the unfulfillment of quality assurance and leads to an increase amount of radiation dose received by the patient. Previous studies have shown that β-carotene mucoadhesive gingival patch can penetrate mucous membrane and provide protection against radiation by reducing the number of gingival epithelial cells micronuclei. The aims of this study was to observe β-carotene mucoadhesive gingival patch effect in GCF volume from patient exposed to repeated periapical radiographs. We recruited 10 participants from patients who receive repeated periapical radiographs in instalation of dentomaxillofacial radiology, Prof Soedomo dental and oral hospital Faculty of Dentistry UGM. The teeth of the subjects are divided into control and treatment group. β-carotene mucoadhesive gingival patch was applied to treatment group. GCF was collected using an absorbing paper strip before and after exposure, then measured bysliding caliper. Paired T-test showed significant differences (p<0.05) between GCF volume before and after radiographic exposure in each group. Independent T-test showed significant differences (p<0.05) of GCF volume between control and treatment group. Conclusion of this study is β-carotene mucoadhesive gingival patch significantly reduce GCF volume after repeated periapical radiographic exposure.

scholarly journals Effects of Antimicrobial Photodynamic Therapy and Local Administration of Minocycline on Clinical, Microbiological, and Inflammatory Markers of Periodontal Pockets: A Pilot Study

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Takahiro Hokari ◽  
Toshiya Morozumi ◽  
Yasutaka Komatsu ◽  
Taro Shimizu ◽  
Toshiaki Yoshino ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Gingival Crevicular Fluid ◽  
Interleukin 1 ◽  
Interferon Γ ◽  
Local Administration ◽  
Clinical Parameters ◽  
Antimicrobial Photodynamic Therapy ◽  
Local Host ◽  
Crevicular Fluid ◽  
Clinical Trials Registry

Objective. We evaluated the efficacies of antimicrobial photodynamic therapy (aPDT) and minocycline ointment (MO) on clinical and bacteriological markers and the local host inflammatory response. Materials and Methods. A total of 30 patients with chronic periodontitis were randomly assigned to two groups. Selected periodontal pockets (probing depth 5–7 mm with bleeding on probing) were treated with aPDT or MO. Measurements of clinical parameters and the collection of gingival crevicular fluid (GCF) and subgingival plaque were performed at baseline, and at 1 and 4 weeks after treatment. Quantification of periodontopathic bacteria in the sulcus and a multiplex bead immunoassay of ten inflammatory cytokines in the GCF were performed. Results. Local MO administration exhibited a significant decrease in scores for clinical parameters (P<0.01) and a significant reduction in bacterial counts (P<0.01) and interleukin-1β and interferon-γ levels at 1 and 4 weeks after treatment (P<0.01). No significant changes were observed in the aPDT group, except in clinical parameters. Conclusions. Although our study had some limitations, we found that while local administration of MO may slightly help to improve clinical, microbiological, and crevicular cytokine levels in periodontal pockets, aPDT did not show any effects. This trial is registered with the UMIN Clinical Trials Registry UMIN000013376.

Antimicrobial Photodynamic Therapy in the Non-Surgical Treatment of Aggressive Periodontitis: Cytokine Profile in Gingival Crevicular Fluid, Preliminary Results

2009 ◽  
Vol 80 (1) ◽  
pp. 98-105 ◽  
Author(s):  
Rafael Ramos de Oliveira ◽  
Humberto Oswaldo Schwartz-Filho ◽  
Arthur Belém Novaes ◽  
Gustavo Pompermaier Garlet ◽  
Raphael Freitas de Souza ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Surgical Treatment ◽  
Gingival Crevicular Fluid ◽  
Aggressive Periodontitis ◽  
Cytokine Profile ◽  
Antimicrobial Photodynamic Therapy ◽  
Preliminary Results ◽  
Crevicular Fluid ◽  
Non Surgical Treatment

scholarly journals Biological changes after dental panoramic exposure: conventional versus digital

2018 ◽  
Vol 51 (1) ◽  
pp. 25
Author(s):  
Rurie Ratna Shantiningsih ◽  
Silviana Farrah Diba
Keyword(s):  
Gingival Crevicular Fluid ◽  
Biological Effects ◽  
Panoramic Radiograph ◽  
Conventional Radiography ◽  
Radiographic Technique ◽  
Significant Difference ◽  
Before And After ◽  
The Difference ◽  
Crevicular Fluid ◽  
Dental Digital Radiography

Background: Dental digital radiography is more practical and requires fewer doses of radiation than conventional radiography. Because ionizing radiation has a biological effect on exposed tissue, concerns regarding its stochastic effect merit greater attention. In a previous study, it was found that biological changes and increases in the micronucleus occurred after conventional panoramic exposure to gingival crevicular fluid (GCF). Purpose: The purpose of this study is to investigate the difference in biological effects after digital panoramic exposure compared with conventional exposure. Methods: Twenty subjects were classified into two groups according to the radiographic technique employed. The techniques consisted of ten subjects undergoing digital panoramic radiograph exposure and ten others being subjected to conventional exposure. GCF calculated in mm3 was collected by applying paper strips to the anterior maxillary labial gingival sulcus for one minute prior to and ten minutes after exposure. A micronucleus was obtained from a gingival smear on the same quadrant ten days after panoramic exposure. Results: There was a significant difference in the number of micronuclei between conventional and digital panoramic radiographs both before and after exposure (p=0.000). In contrast, increased GCF volume was not statistically significant (p=0.506) before or after digital panoramic exposure, while the significant difference of conventional panoramic exposure was p=0.017. Conclusion: Digital panoramic radiograph exposure induced a biological change only in terms of an increase in the number of micronuclei but not in the volume of GCF.

scholarly journals Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 869
Author(s):  
Beatriz Müller Nunes Souza ◽  
Juliana Guerra Pinto ◽  
André Henrique Correia Pereira ◽  
Alejandro Guillermo Miñán ◽  
Juliana Ferreira-Strixino
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Bacterial Inactivation ◽  
Bacterial Reduction ◽  
Complete Inactivation ◽  
Opportunistic Bacteria ◽  
Significant Difference ◽  
Mrsa Strains

Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.

scholarly journals OHIS Differences on Primary School Students in Relation to Little Dentist Training

2021 ◽  
Author(s):  
Asep Arifin Senjaya ◽  
Ni Made Sirat ◽  
I Nyoman Wirata ◽  
Ni Ketut Ratmini
Keyword(s):  
Treatment Group ◽  
Primary School ◽  
Oral Hygiene ◽  
Dental Health ◽  
Control Group ◽  
Primary School Students ◽  
School Students ◽  
Significant Difference ◽  
Before And After ◽  
The Difference

Dental healthcare is very important since dental and oral hygiene should be maintained. Statistics showed that more than 80% of children in developed and developing countries suffer from dental disease. This study aimed to see the safety of OHIS (Oral Hygiene Index Simplified) in primary school students who got and did not get little dentist cadre training in Bangli Regency in 2019. The study was done in an experimental design: pre and post-test with control design, which was conducted in August-September 2019. The sample in this study is 366 students. The difference in OHIS scores before and after treatment in the control group and treatment group was carried out by the bivariate Mann Whitney U Test. The results of the study showed that before dental health training was conducted, there were 54.3% of primary school students in the treatment group with good OHIS score criteria, and after the training was carried out as many as 98.4% of the students in the treatment group had a good OHIS. Additionally, prior to the training, 57.5% of the control group had fair OHIS criteria score. Then, after the training was carried out, 73.7% of the control group had good OHIS score. Hence, the study concluded that there was a significant difference in the OHIS of primary school students who got little doctor training prior to and after the training. Also, there was a significant difference in the OHIS of the students who did not get such treatment before and after the training.

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