Missing Nurse Bees—Early Transcriptomic Switch From Nurse Bee to Forager Induced by Sublethal Imidacloprid

The environmental residue/sublethal doses of neonicotinoid insecticides are believed to generate a negative impact on pollinators, including honey bees. Here we report our recent investigation on how imidacloprid, one of the major neonicotinoids, affects worker bees by profiling the transcriptomes of various ages of bees exposed to different doses of imidacloprid during the larval stage. The results show that imidacloprid treatments during the larval stage severely altered the gene expression profiles and may induce precocious foraging. Differential expression of foraging regulators was found in 14-day-old treated adults. A high transcriptome similarity between larvae-treated 14-day-old adults and 20-day-old controls was also observed, and the similarity was positively correlated with the dose of imidacloprid. One parts per billion (ppb) of imidacloprid was sufficient to generate a long-term impact on the bee’s gene expression as severe as with 50 ppb imidacloprid. The disappearance of nurse bees may be driven not only by the hive member constitution but also by the neonicotinoid-induced precocious foraging behavior.

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Transcriptome-wide gene expression plasticity in Stipa grandis in response to grazing intensity differences

10.22541/au.160655409.95797246/v1 ◽

2020 ◽

Author(s):

Zhenhua Dang ◽

Yuanyuan Jia ◽

Yunyun Tian ◽

Jiabin Li ◽

Yanan Zhang ◽

...

Keyword(s):

Gene Expression ◽

Metabolic Pathways ◽

Molecular Mechanisms ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Grazing Intensity ◽

Glycolate Oxidase ◽

Bisphosphate Carboxylase ◽

Grazing Intensities

Organisms have evolved effective and distinct adaptive strategies to survive. Stipa grandis is one of the widespread dominant species on the typical steppe of the Inner Mongolian Plateau, and is regarded as a suitable species for studying the effects of grazing in this region. Although phenotypic (morphological and physiological) variations in S. grandis in response to long-term grazing have been identified, the molecular mechanisms underlying adaptations and plastic responses remain largely unknown. Accordingly, we performed a transcriptomic analysis to investigate changes in gene expression of S. grandis under four different grazing intensities. A total of 2,357 differentially expressed genes (DEGs) were identified among the tested grazing intensities, suggesting long-term grazing resulted in gene expression plasticity that affected diverse biological processes and metabolic pathways in S. grandis. DEGs were identified that indicated modulation of Calvin–Benson cycle and photorespiration metabolic pathways. The key gene´expression profiles encoding various proteins (e.g., Ribulose-1,5-bisphosphate carboxylase/oxygenase, fructose-1,6-bisphosphate aldolase, glycolate oxidase etc.) involved in these pathways suggest that they may synergistically respond to grazing to increase the resilience and stress tolerance of S. grandis. Our findings provide scientific clues for improving grassland use and protection, and identify important questions to address in future transcriptome studies.

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Long-term affected flat oyster (Ostrea edulis) haemocytes show differential gene expression profiles from naïve oysters in response to Bonamia ostreae

Genomics ◽

10.1016/j.ygeno.2018.04.002 ◽

2018 ◽

Vol 110 (6) ◽

pp. 390-398 ◽

Cited By ~ 5

Author(s):

P. Ronza ◽

A. Cao ◽

D. Robledo ◽

A. Gómez-Tato ◽

J.A. Álvarez-Dios ◽

...

Keyword(s):

Gene Expression ◽

Differential Gene Expression ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Ostrea Edulis ◽

Bonamia Ostreae ◽

Differential Gene ◽

Flat Oyster

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Meta-analysis of gene expression profiles in long-term non-progressors infected with HIV-1

BMC Medical Genomics ◽

10.1186/s12920-018-0443-x ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 4

Author(s):

Sun Young Lee ◽

Yong Kwang Park ◽

Cheol-Hee Yoon ◽

Kisoon Kim ◽

Kyung-Chang Kim

Keyword(s):

Gene Expression ◽

Expression Profiles ◽

Meta Analysis ◽

Gene Expression Profiles ◽

Hiv 1

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Identification of Monotonically Differentially Expressed Genes for IFN-β-Treated Multiple Sclerosis Patients

BioMed Research International ◽

10.1155/2019/5647902 ◽

2019 ◽

Vol 2019 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Suyan Tian ◽

Lei Zhang

Keyword(s):

Gene Expression ◽

Multiple Sclerosis ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Biologically Relevant ◽

Therapeutic Mechanism ◽

The Central Nervous System ◽

Multiple Sclerosis Patients ◽

Term Response

Multiple sclerosis (MS) is a common neurological disability of the central nervous system. Immune-modulatory therapy with interferon-β (IFN-β) has been used as a first-line treatment to prevent relapses in MS patients. While the therapeutic mechanism of IFN-β has not been fully elucidated, the data of microarray experiments that collected longitudinal gene expression profiles to evaluate the long-term response of IFN-β treatment have been analyzed using statistical methods that were incapable of dealing with such data. In this study, the GeneRank method was applied to generate weighted gene expression values and the monotonically expressed genes (MEGs) for both IFN-β treatment responders and nonresponders were identified. The proposed procedure identified 13 MEGs for the responders and 2 MEGs for the nonresponders, most of which are biologically relevant to MS. Our work here provides some useful insight into the mechanism of IFN-β treatment for MS patients. A full understanding of the therapeutic mechanism will enable a more personalized treatment strategy possible.

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