scholarly journals Genome-Wide Analysis of TIR-NBS-LRR Gene Family in Potato Identified StTNLC7G2 Inducing Reactive Oxygen Species in Presence of Alternaria solani

2022 ◽  
Vol 12 ◽  
Author(s):  
Namo Dubey ◽  
Anjali Chaudhary ◽  
Kunal Singh
Keyword(s):  
Reactive Oxygen Species ◽  
Gene Family ◽  
Functional Characterization ◽  
Reactive Oxygen ◽  
Alternaria Solani ◽  
Susceptible Variety ◽  
Chromosome 1 ◽  
Post Inoculation ◽  
Oxygen Species ◽  
Chromosome 11

Resistance gene analogs (RGAs) comprising NBS-LRR gene family members are considered prominent candidates in the development of disease-resistant genotypes. NBS-LRR gene family comprised a very large number of genes; therefore, members of one subfamily TIR-NBS-LRR (TNL) are identified in the present study from Solanum tuberosum genome, followed by their bioinformatics characterization. The study identified a total of 44 genes encoding 60 TNL transcripts with two prominent clusters at chromosome 1 and chromosome 11. Expression analysis of 14 TNL genes after Alternaria solani infection at 1, 2, 3, 5, and 7 days post inoculation in two disease-tolerant varieties, Kufri Jyoti and Kufri Pukhraj, and one relatively susceptible variety, Kufri Chandramukhi, showed differential expression of many genes including a high expression (>15-fold) of StTNLC6G2T1 and StTNLC11G9T1. Functional characterization of one such gene, StTNLC7G2, reveals involvement in the generation of reactive oxygen species under A. solani attack, implicating its putative role in plant defense via hypersensitive response.

scholarly journals Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to Blumeria graminis f. sp. tritici in Wheat

2020 ◽  
Vol 21 (16) ◽  
pp. 5767
Author(s):  
Yuting Hu ◽  
Shengfu Zhong ◽  
Min Zhang ◽  
Yinping Liang ◽  
Guoshu Gong ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Salicylic Acid ◽  
Powdery Mildew ◽  
Reactive Oxygen ◽  
Post Inoculation ◽  
Oxygen Species ◽  
Chlorophyll Fluorescence Parameters ◽  
H2o2 Accumulation ◽  
Pathogenesis Related ◽  
Defence Responses

Photosynthesis is not only a primary generator of reactive oxygen species (ROS) but also a component of plant defence. To determine the relationships among photosynthesis, ROS, and defence responses to powdery mildew in wheat, we compared the responses of the Pm40-expressing wheat line L658 and its susceptible sister line L958 at 0, 6, 12, 24, 48, and 72 h post-inoculation (hpi) with powdery mildew via analyses of transcriptomes, cytology, antioxidant activities, photosynthesis, and chlorophyll fluorescence parameters. The results showed that H2O2 accumulation in L658 was significantly greater than that in L958 at 6 and 48 hpi, and the enzymes activity and transcripts expression of peroxidase and catalase were suppressed in L658 compared with L958. In addition, the inhibition of photosynthesis in L658 paralleled the global downregulation of photosynthesis-related genes. Furthermore, the expression of the salicylic acid-related genes non-expressor of pathogenesis related genes 1 (NPR1), pathogenesis-related 1 (PR1), and pathogenesis-related 5 (PR5) was upregulated, while the expression of jasmonic acid- and ethylene-related genes was inhibited in L658 compared with L958. In conclusion, the downregulation of photosynthesis-related genes likely led to a decline in photosynthesis, which may be combined with the inhibition of peroxidase (POD) and catalase (CAT) to generate two stages of H2O2 accumulation. The high level of H2O2, salicylic acid and PR1 and PR5 in L658 possible initiated the hypersensitive response.

scholarly journals Reactive oxygen species and the regulation of cell death by the Bcl-2 gene family

1995 ◽  
Vol 1271 (1) ◽  
pp. 63-66 ◽  
Author(s):  
Stanley J. Korsmeyer ◽  
Xiao-Ming Yin ◽  
Zoltán N. Oltvai ◽  
Deborah J. Veis-Novack ◽  
Gerald P. Linette
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Gene Family ◽  
Reactive Oxygen ◽  
Oxygen Species

Functional characterization of a reactive oxygen species modulator 1 gene in Litopenaeus vannamei

2017 ◽  
Vol 70 ◽  
pp. 270-279 ◽  
Author(s):  
Hong-Hui He ◽  
Yi-Miao Chi ◽  
Kai Yuan ◽  
Xiao-Yun Li ◽  
Shao-Ping Weng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Litopenaeus Vannamei ◽  
Functional Characterization ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals The Peptide PbrPSK2 From Phytosulfokine Family Induces Reactive Oxygen Species (ROS) Production to Regulate Pear Pollen Tube Growth

2020 ◽  
Vol 11 ◽  
Author(s):  
Xiaobing Kou ◽  
Qian Liu ◽  
Yangyang Sun ◽  
Peng Wang ◽  
Shaoling Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Gene Family ◽  
Pollen Tube Growth ◽  
Prunus Persica ◽  
Reactive Oxygen ◽  
Tube Growth ◽  
Evolutionary Analysis ◽  
Dependent Manner ◽  
Oxygen Species

Phytosulfokines (PSKs) are plant peptide growth factors that participate in multiple biological processes, including cell elongation and immune signaling. However, little is known about PSKs in Rosaceae species. Here, we identified 10 PSK genes in pear (Pyrus bretschneideri), 11 in apple (Malus × domestica), four in peach (Prunus persica), six in strawberry (Fragaria vesca), and five in Chinese plum (Prunus mume). In addition, we undertook comparative analysis of the PSK gene family in pear and the four other species. Evolutionary analysis indicated that whole genome duplication events (WGD) may have contributed to the expansion of the PSK gene family in Rosaceae. Transcriptomes, reverse transcription-PCR and quantitative real-time-PCR analyses were undertaken to demonstrate that PbrPSK2 is highly expressed in pear pollen. In addition, by adding purified E. coli-expressed PbrPSK2 to pollen and using an antisense oligonucleotide approach, we showed that PbrPSK2 can promote pear pollen tube elongation in a dose-dependent manner. Furthermore, PbrPSK2 was found to mediate the production of reactive oxygen species to regulate pear pollen tube growth.

Mitochondria as a source of reactive oxygen species

2009 ◽  
pp. c3 ◽  
Author(s):  
Helena M. Cochemé ◽  
Michael P. Murphy
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species

Clinical aspects of reactive oxygen and nitrogen species

2004 ◽  
Vol 71 ◽  
pp. 121-133 ◽  
Author(s):  
Ascan Warnholtz ◽  
Maria Wendt ◽  
Michael August ◽  
Thomas Münzel
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Endothelial Dysfunction ◽  
Vascular Tissue ◽  
Rapid Development ◽  
Reactive Oxygen ◽  
Clinical Aspects ◽  
No Production ◽  
Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

Fas ligand expression in rat kupffer cells in response to lipopolysaccharide is mediated by reactive oxygen species

2001 ◽  
Vol 120 (5) ◽  
pp. A361-A361
Author(s):  
K UCHIKURA ◽  
T WADA ◽  
Z SUN ◽  
S HOSHINO ◽  
G BULKLEY ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Kupffer Cells ◽  
Fas Ligand ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Ligand Expression
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