scholarly journals The Association of HLA-G Gene Polymorphism and Its Soluble Form With Male Infertility

2022 ◽  
Vol 12 ◽  
Author(s):  
Karolina Piekarska ◽  
Paweł Radwan ◽  
Agnieszka Tarnowska ◽  
Andrzej Wiśniewski ◽  
Rafał Krasiński ◽  
...  
Keyword(s):  
Male Infertility ◽  
Human Leukocyte ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Test Material ◽  
Soluble Form ◽  
Accessory Glands ◽  
Normal Sperm ◽  
Soluble Hla

Successful reproduction depends on many factors. Male factors contribute to infertility in approximately 50% of couples who fail to conceive. Seminal plasma consists of secretions from different accessory glands containing a mixture of various cytokines, chemokines, and growth factors, which together can induce a local immune response that might impact on a male’s as well as a female’s fertility. Human leukocyte antigen (HLA)-G expression has been suggested as an immunomodulatory molecule that influences pregnancy outcome. The HLA-G gene encodes either membrane-bound or/and soluble proteins. The aim of this study was the evaluation of HLA-G polymorphisms and their impact on soluble HLA-G (sHLA-G) production. We tested the HLA-G polymorphism in three positions: rs1632947: c.-964G>A; rs1233334: c.-725G>C/T in the promoter region; rs371194629: c.∗65_∗66insATTTGTTCATGCCT in the 3′ untranslated region. We tested two cohorts of men: 663 who participated in in vitro fertilization (test material was blood or sperm), and 320 fertile controls who possessed children born after natural conception (test material was blood). Since 50% of men visiting assisted reproductive clinics have abnormal semen parameters, we wondered if men with normal sperm parameters differ from those with abnormal parameters in terms of HLA-G polymorphism and secretion of sHLA-G into semen. We found that certain rs1632947-rs1233334-rs371194629 HLA-G haplotypes and diplotypes were associated with male infertility, while others were protective. Normozoospermic men with the A-C-del haplotype and A-C-del/A-C-del diplotype secreted the most sHLA-G into semen (574.1 IU/mL and 1047.0 IU/mL, respectively), while those with the G-C-ins haplotype and G-C-ins/G-C-ins diplotype – the least (80.8 IU/mL and 75.7 IU/mL, respectively). Men with the remaining haplotypes/diplotypes secreted sHLA-G at an intermediate level. However, only in one haplotype, namely G-C-ins, did we observe strong significant differences in the concentration of sHLA-G in the semen of men with teratozoospermia compared to men with normal sperm parameters (p = 0.009). In conclusion, fertile men differ in the profile of HLA-G polymorphism from men participating in IVF. Among all HLA-G haplotypes, the most unfavorable for male fertility is the G-C-ins haplotype, which determines the secretion of the lowest concentration of the soluble HLA-G molecule. This haplotype may reduce sperm parameters.

scholarly journals Review of Clinical Trials on Effects of Oral Antioxidants on Basic Semen and Other Parameters in Idiopathic Oligoasthenoteratozoospermia

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Senka Imamovic Kumalic ◽  
Bojana Pinter
Keyword(s):  
Clinical Trials ◽  
Male Infertility ◽  
Diagnostic Methods ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Male Factor ◽  
Infertile Men ◽  
Antioxidant Supplements ◽  
Year 2000

Infertility affects 50 to 80 million people worldwide. Male factor is a cause of infertility in almost half of cases, mainly due to oligoasthenoteratozoospermia (OAT). With common diagnostic methods no cause can be found in approximately 30% of cases of male infertility due to OAT and these are considered idiopathic. Reactive oxygen species (ROS) play an important role in male infertility and are proved to be higher in infertile men; antioxidants could oppose their effect. The aim of this paper was to review the literature on clinical trials in the period from year 2000 to year 2013 studying the effects of various types of antioxidant supplements on basic and other sperm parameters and pregnancy rates in subfertile males with idiopathic OAT. The majority of studies were randomized and placebo controlled and confirmed beneficial effect of antioxidants on at least one of the semen parameters; the biggest effect was determined on sperm motility. In many of these trials combinations of more antioxidants were assessed. The optimal dosages of one or more antioxidants were not defined. We concluded that antioxidants play an important role in protecting semen from ROS and can improve basic sperm parameters in case of idiopathic OAT.

scholarly journals Male Infertility and Sperm DNA Fragmentation

2018 ◽  
Vol 6 (8) ◽  
pp. 1342-1345 ◽  
Author(s):  
Afrim Zeqiraj ◽  
Sheqibe Beadini ◽  
Nexhbedin Beadini ◽  
Hesat Aliu ◽  
Zafer Gashi ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Reproductive Age ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Control Group ◽  
P Value ◽  
Sperm Dna Fragmentation ◽  
Factors Affecting

BACKGROUND: One of the main factors affecting male infertility is DNA fragmentation in sperm. Male infertility is a heterogeneous group of disorders, known causes account for only 30-50%, and unknown cause (idiopathic) constitute the rest. Infertility involves nearly 15% of couples in the reproductive age, and only the male problem involves about 40% of the problems.AIM: We have studied our DNA damage to sperm cells of a group of infertile males (113 patients) with abnormal sperm parameters (oligoasthenospermia and oligospermia) and a group of male patients (80 patients) with normal semen parameters (normospermia) to document whether the Sperm Chromatin Dispersion (SCD) analysis could increase the information obtained from the sperm routine analysis to explain the causes of infertility.MATERIALS: A group of 193 patients were analysed, 113 patients in the working group and 80 patients in the control group were screened. The ejaculate samples were taken by the patient to whom the reason for the analysis was explained. All patients were from the Republic of Kosovo. Samples are collected from 2014/2018. Sperm Chromatin Dispersion (SCD) analyses in the ejaculate were analysed by the Biolab Zafi laboratory in Peja.RESULTS: Clinical data were compared between the two groups by one-way ANOVA, mean ± SD, student's t-test. A p-value of less than P < 0.05% was considered statistically significant. Outcomes: In our study, we have gained significant (P < 0.05) results in the workgroup and the control group across all hormonal parameters, sperm parameters, and fragmented DNA in the sperm.CONCLUSION: Based on our obtained results we can conclude that DNA fragmentation in spermatozoa is useful in the selection of unsuitable DNA sperm for use in ART methods. We conclude that our DNA fragmentation analysis results are encouraging and can be used for diagnostic purposes in determining male infertility.

Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation

Blood ◽  
2006 ◽  
Vol 109 (7) ◽  
pp. 2806-2814 ◽  
Author(s):  
Stéphanie Coupel ◽  
Anne Moreau ◽  
Mohamed Hamidou ◽  
Vaclav Horejsi ◽  
Jean-Paul Soulillou ◽  
...  
Keyword(s):  
Cell Activation ◽  
Systemic Vasculitis ◽  
Human Leukocyte ◽  
Serum Levels ◽  
Antineutrophil Cytoplasmic Antibody ◽  
Cell Surface Expression ◽  
Endothelial Cell Activation ◽  
Cellular Expression ◽  
Soluble Hla

AbstractHuman leukocyte antigen (HLA)–E belongs, with HLA-G and HLA-F, to the nonclassic major histocompatibility complex (MHC) class I (Ib) molecules, broadly defined by a limited polymorphism and a restricted pattern of cellular expression. In contrast to HLA-G, the expression and function of HLA-E and HLA-F in physiologic and pathologic processes remain poorly established. In the present study, we show that HLA-E protein expression in normal human nonlymphoid organs is mainly restricted to endothelial cells (ECs). HLA-E is also basally expressed by B and T lymphocytes, natural killer (NK) cells and by macrophages. We demonstrate that tumor necrosis factor α (TNFα), interleukin-1β (IL-1β), and interferon γ (IFNγ) up-regulate the cell-surface expression of HLA-E on ECs in vitro and induce the release of soluble HLA-E (sHLA-E). HLA-E up-regulation protects IFNγ-activated ECs from NK-mediated cell lysis, while sHLA-E protects bystander cells. Finally, sHLA-E is not detected in normal sera, and increased serum levels correlate with disease activity in patients with antineutrophil cytoplasmic antibody–associated systemic vasculitis. Thus, HLA-E expression and release of sHLA-E are features of EC activation and emphasize immunoregulatory functions of the endothelium. The present identification of soluble HLA-E molecules may have important implications in understanding the pathogenesis of immune-mediated vascular diseases and for the diagnosis and monitoring of patients.

scholarly journals Clinical significance of sperm morphology in male infertility treatment

2021 ◽  
Vol 14 (3) ◽  
pp. 127-132
Author(s):  
Yu.V. Olefir ◽  
◽  
D.M. Monakov ◽  
◽  
Keyword(s):  
Male Infertility ◽  
Clinical Significance ◽  
Sperm Morphology ◽  
Assisted Reproductive Technologies ◽  
Intrauterine Insemination ◽  
Reproductive Technologies ◽  
Vitro Fertilization ◽  
Normal Sperm ◽  
Starting Point

Introduction. Sperm morphology is one of the most common tests in fertility practice. The interlaboratory variability is the main drawback of the method. The clinical significance of sperm morphology in assisted reproductive technologies is controversial. The aim of the review is to address this question. Materials and methods. The search of relevant publications was carried out in PubMed and e-Library databases using the keywords «male infertility», «sperm morphology», «teratozoospermia», «IUI», «IVF», «ICSI». Conference abstracts and dissertations were excluded from analysis and 56 publications were included in this literature review. Results. The small numbers of studies were evaluated to the effect of teratozoospermia on the likelihood of natural pregnancy. The pregnancy rate was higher in the group of couples with normozoospermia. In the couples with severe teratozoospermia pregnancies rate was also detected. The most studies did not reveal a statistically significant effect of tertozoospermia on the frequency of pregnancy during intrauterine insemination. The data about the influence of sperm morphology on in vitro fertilization are contradictory. Early studies showed a positive correlation between normal sperm morphology and frequency of conception, but these results were not confirmed in further studies. The most studies have not been revealed the correlation between normal sperm morphology male fertility status, clinical and live birth rate. Discussion. To date the spermatozoa mofophology studying remains the «starting point» ofa man's examination for infertility. However, the data available do not confirm its role in choosing the method ofassisted reproductive technologies or predicting their results. Conclusions. To date the role of sperm morphology on conceive and pregnancy frequencies in ART use is controversal. The data available does not confirm the value of this test as a proxy of higher pregnancy and birth of healthy child probability. The further studies are required to address this question.

scholarly journals An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section I

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3248
Author(s):  
Morgan F. Orsolini ◽  
Stuart A. Meyers ◽  
Pouya Dini
Keyword(s):  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Sperm Parameters ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Embryo Production ◽  
Normal Sperm ◽  
Production Section ◽  
Equine Industry

As the use of assisted reproductive technologies (ART) and in vitro embryo production (IVP) expand in the equine industry, it has become necessary to further our understanding of semen physiology as it applies to overall fertility. This segment of our two-section review will focus on normal sperm parameters, beginning with development and extending through the basic morphology of mature spermatozoa, as well as common issues with male factor infertility in IVP. Ultimately, the relevance of sperm parameters to overall male factor fertility in equine IVP will be assessed.

scholarly journals Sperm Lipid Peroxidation and Antioxidants Activities as Predictors of Pregnancy Outcomes after Density Gradient Centrifugation in Medical Assistance Procreation

2020 ◽  
pp. 173-182
Author(s):  
Hasnae Debbarh ◽  
Smahane Aboulmaouahib ◽  
Malak Jamil ◽  
Khadija Mounaji ◽  
Omar Sefrioui ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Sperm Parameters ◽  
Antioxidant Enzyme Activities ◽  
Vitro Fertilization ◽  
Normal Sperm ◽  
Abnormal Sperm

Aims: The purpose of this study was to assess the Density Gradient Centrifugation (DGC) effectiveness in spermatozoa selection by analyzing the oxidative stress profile and in pregnancy prediction outcomes of In Vitro Fertilization (IVF). Study Design: This was a prospective study. Place and Duration of Study: Department of Biology, laboratory of Physiopathology Molecular Genetic and Biotechnology. Ain Chock Faculty of Sciences, Hassan II University, Casablanca. And Anfa Fertility Center Private Clinic of in vitro fertilization and Endoscopic Surgery, Casablanca. Methodology: We included 101 men patients subdivided into three groups. Group A with normal sperm parameters and a positive pregnancy; Group B with normal sperm parameters and a negative pregnancy; Group C with abnormal sperm parameters and a negative pregnancy. After DGC, lipid peroxidation (MDA) level, as well as the antioxidant enzyme activities of superoxide dismutase (SOD), glutathione reductase (GR) and catalase (CAT), were evaluated. Results: Patients with normal and abnormal sperm parameters showed that MDA level, and antioxidant enzymes activities increased significantly from the pellet, to 80 %, and from 80% to 40% fractions. In addition, lipid peroxidation and enzymes activities levels were significantly lower in patients with positive pregnancy than in patients with negative pregnancy and especially those with abnormal spermatic parameters. Conclusion: The DGC would select sperm not only motile and viable but with less oxidative stress. Moreover, the rate of lipid peroxidation and antioxidant enzyme activities could differentiate between patients with normal and abnormal spermatic parameters and between patients with positive and negative pregnancy outcome.

scholarly journals Impact of Cigarette Smoking on Sperm Parameters of Infertile Men in Center of Algiers (Capital of Algeria)

2020 ◽  
Vol 10 (4-s) ◽  
pp. 193-196
Author(s):  
Amina Itatahine ◽  
Abbassia Demmouche ◽  
H Maï ◽  
Z.C. Khalloua ◽  
D. Ferrag ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Male Infertility ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Negative Effects ◽  
Negative Effect ◽  
Pass Through ◽  
And Function

Background : Cigarette smoking has negative effects on male fertility. Toxins from tobacco smoking can potentially affect sperm development and function, with a negative effect on semen parameters. In fact, the components of cigarette smoke pass through the blood-testis barrier and thus induce an alteration of sperm parameters and nucleus quality of the spermatozoa. The aim is to determine a diffence between tobbaco and no tobbaco patient and semen parameters. This article aimed to assess the relationship between smoking and male infertility. Subject and Methods : this is retrospective study focused on the effects of smoking on semen analyses.  377 men (165 non-smokers, 212  smokers) with infertility for at least 1 year were evaluated between  Mars  and  November 2018, in center of algiers (capital of Algeria). Sperm characteristics (concentration, motility, morphology, and volume) were detemined. Results: Sperm concentration, percentage motility and morphology were significantly lower in an infertile Smokers group than the infertile Nonsmokers group. We also observed that the infertility duration were significantly increased (p<0.05) in accordance with the age in an infertile Smokers group.  Our study shows that cigarette smoking is associated with reduced sperm count and motility. Conclusions: Our results suggest that cigarette smoking has an overall negative effect on semen parameters. Our study suggests that men should be advised to abstain from smoking in order to improve reproductive outcomes. Keywords: smoking, semen, male infertility, sperm, humans

scholarly journals Date Palm Pollen versus Pentoxifylline on Improvement of Sperm Parameters in Idiopathic Male Infertility

2020 ◽  
Author(s):  
Mohammadreza Mirzaei ◽  
Firoozeh Akbari Asbagh ◽  
Majid Safavi ◽  
Mir Saeed Yekaninejad ◽  
Roja Rahimi ◽  
...  
Keyword(s):  
Male Infertility ◽  
Traditional Medicine ◽  
Date Palm ◽  
Reproductive Age ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Idiopathic Male Infertility ◽  
Iranian Traditional Medicine ◽  
Sperm Counts ◽  
Date Palm Pollen

Abstract Background: Idiopathic male infertility (IMI) affects nearly 30 to 45% of men in their optimal reproductive age. In this regard, there are no evidence-based medications prescribed empirically to treat IMI. However, applying traditional medicine for the treatment of male infertility has attracted more attention in recent years. In the current study, the effects of date palm pollen (DPP) on sperm parameters in patients with idiopathic infertility were evaluated and compared to pentoxifylline (PTX). Materials and methods: This study was performed on 80 adult male individuals (20-35 years old) who had oligozoospermia and/or asthenozoospermia and/or teratozoospermia. Patients received 6g DPP powder or 400 mg PTX tablets daily for three months in two separated groups (each in 40). Various sperm parameters and levels of sex hormones were measured. Results: Compared to the PTX group, results revealed a significant improvement in sperm counts (p=0.016), morphology (p=0.029), total motility (p=0.018), progressive motility (p=0.016) and reduction of immotile sperms (p=0.014) in patients who received DPP treatment. Conclusion Iranian Traditional Medicine (ITM) strongly recommended DPP as a therapeutic agent to cure IMI through improvement in the functional competence of sperm and semen parameters.

IVF and male infertility

1992 ◽  
Vol 1 (2) ◽  
pp. 151-164 ◽  
Author(s):  
Susan M Avery
Keyword(s):  
Male Infertility ◽  
Semen Quality ◽  
Semen Analysis ◽  
World Health ◽  
Semen Parameters ◽  
Infertile Men ◽  
Health Organization ◽  
Sperm Dysfunction

Male infertility, while having a variety of causes, is generally discussed in terms of semen parameters. While the World Health Organization (WHO) have been able io set limits for semen parameters below which a male can be considered subfertile (20 million/ml; >50% motility; >50% morphologically normal forms), it is well documented thatin vivoconceptions have been achieved where semen quality falls well outside these limits, and that infertile men may have normal semen parameters. Macleod and Gold in comparing 1000 fertile men and 1000 infertile men, found that significantly more infertile men had sperm densities below 20 million/ml, but also that 60% of infertile men had sperm densities of 60 million or more. Jouannet and Feneaux have shown that the conception ratein vivoonly apparently falls significantly at sperm concentrations of less than five million/ml. Although the cause of subnormal semen analysis is unknown in the majority of cases, there is no reason to suppose that abnormal semen parameters on their own are the cause of infertility. Rather the problem may be caused by failure of sufficient numbers of sperm traversing the female tract and reaching the oocyte. Unfortunately, lack of defined diagnoses lead to a lack of direct treatment for subnormal semen parameters. The development ofin vitrofertilization (IVF) resulted in a method that could be used to circumvent the problem since it requires relatively low numbers of sperm and these are placed in the immediate vicinity of the oocyte. It should also be pointed out that normal semen parameters do not imply fertility, since these parameters cannot directly identify dysfunction. IVF offers the advantage that sperm-oocyte interractions can be observed, and in cases of fertilization failure, the point at which sperm dysfunction manifests itself may potentially be identified – if not the nature of the dysfunction. Techniques have now been developed that may overcome certain types of dysfunction, using both biochemical and mechanical means.

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