scholarly journals Birds Kept in the German Zoo “Tierpark Berlin” Are a Common Source for Polyvalent Yersinia pseudotuberculosis Phages

2022 ◽  
Vol 12 ◽  
Author(s):  
Jens Andre Hammerl ◽  
Andrea Barac ◽  
Anja Bienert ◽  
Aslihan Demir ◽  
Niklas Drüke ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Host Range ◽  
Yersinia Pseudotuberculosis ◽  
Common Source ◽  
Broad Host Range ◽  
Fecal Samples ◽  
The Third ◽  
Zoo Animals

Yersinia pseudotuberculosis is an important animal pathogen, particularly for birds, rodents, and monkeys, which is also able to infect humans. Indeed, an increasing number of reports have been published on zoo animals that were killed by this species. One option to treat diseased animals is the application of strictly lytic (virulent) phages. However, thus far relatively few phages infecting Y. pseudotuberculosis have been isolated and characterized. To determine the prevalence of Y. pseudotuberculosis phages in zoo animals, fecal samples of birds and some primates, maras, and peccaries kept in the Tierpark Berlin were analyzed. Seventeen out of 74 samples taken in 2013 and 2017 contained virulent phages. The isolated phages were analyzed in detail and could be allocated to three groups. The first group is composed of 10 T4-like phages (PYps2T taxon group: Myoviridae; Tevenvirinae; Tequatrovirus), the second group (PYps23T taxon group: Chaseviridae; Carltongylesvirus; Escherichia virus ST32) consists of five phages encoding a podovirus-like RNA polymerase that is related to an uncommon genus of myoviruses (e.g., Escherichia coli phage phiEcoM-GJ1), while the third group is comprised of two podoviruses (PYps50T taxon group: Autographiviridae; Studiervirinae; Berlinvirus) which are closely related to T7. The host range of the isolated phages differed significantly. Between 5.5 and 86.7% of 128 Y. pseudotuberculosis strains belonging to 20 serotypes were lysed by each phage. All phages were additionally able to lyse Y. enterocolitica B4/O:3 strains, when incubated at 37°C. Some phages also infected Y. pestis strains and even strains belonging to other genera of Enterobacteriaceae. A cocktail containing two of these phages would be able to lyse almost 93% of the tested Y. pseudotuberculosis strains. The study indicates that Y. pseudotuberculosis phages exhibiting a broad-host range can be isolated quite easily from zoo animals, particularly birds.

Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors

Gene ◽  
1983 ◽  
Vol 26 (2-3) ◽  
pp. 273-282 ◽  
Author(s):  
Miroslawa M. Bagdasarian ◽  
Egon Amann ◽  
Rudolf Lurz ◽  
Beate Rückert ◽  
Michael Bagdasarian
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Putida ◽  
Host Range ◽  
Expression Vectors ◽  
Broad Host Range ◽  
Tac Promoter

A new family of mobilizable suicide plasmids based on broad host range R388 plasmid (IncW) and RP4 plasmid (IncPα) conjugative machineries and their cognate Escherichia coli host strains

2005 ◽  
Vol 156 (2) ◽  
pp. 245-255 ◽  
Author(s):  
Gaëlle Demarre ◽  
Anne-Marie Guérout ◽  
Chiho Matsumoto-Mashimo ◽  
Dean A. Rowe-Magnus ◽  
Philippe Marlière ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Host Range ◽  
Broad Host Range ◽  
New Family ◽  
Host Strains

Novel broad host range shuttle vectors for expression in Escherichia coli, Bacillus subtilis and Pseudomonas putida

2012 ◽  
Vol 161 (2) ◽  
pp. 71-79 ◽  
Author(s):  
Sonja Christina Troeschel ◽  
Stephan Thies ◽  
Olga Link ◽  
Catherine Isabell Real ◽  
Katja Knops ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Host Range ◽  
Broad Host Range ◽  
Shuttle Vectors ◽  
Expression In Escherichia Coli

scholarly journals Bio-control of O157:H7, and colistin-resistant MCR-1-positive Escherichia coli using a new designed broad host range phage cocktail

LWT ◽  
2021 ◽  
pp. 112836
Author(s):  
Khashayar Shahin ◽  
Hongduo Bao ◽  
Shujiao Zhu ◽  
Abbas Soleimani-Delfan ◽  
Tao He ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Host Range ◽  
Broad Host Range ◽  
Phage Cocktail

scholarly journals LamB, OmpC, and the Core Lipopolysaccharide of Escherichia coli K-12 Function as Receptors of Bacteriophage Bp7

2020 ◽  
Vol 94 (12) ◽  
Author(s):  
Peipei Chen ◽  
Huzhi Sun ◽  
Huiying Ren ◽  
Wenhua Liu ◽  
Guimei Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Host Range ◽  
Inner Core ◽  
Phage Infection ◽  
Broad Host Range ◽  
Content Type ◽  
E Coli ◽  
Phage Adsorption ◽  
Specific Receptors ◽  
K 12

ABSTRACT Bp7 is a T-even phage with a broad host range specific to Escherichia coli, including E. coli K-12. The receptor binding protein (RBP) of bacteriophages plays an important role in the phage adsorption process and determines phage host range, but the molecular mechanism involved in host recognition of phage Bp7 remains unknown. In this study, the interaction between phage Bp7 and E. coli K-12 was investigated. Based on homology alignment, amino acid sequence analysis, and a competitive assay, gp38, located at the tip of the long tail fiber, was identified as the RBP of phage Bp7. Using a combination of in vivo and in vitro approaches, including affinity chromatography, gene knockout mutagenesis, a phage plaque assay, and phage adsorption kinetics analysis, we identified the LamB and OmpC proteins on the surface of E. coli K-12 as specific receptors involved in the first step of reversible phage adsorption. Genomic analysis of the phage-resistant mutant strain E. coli K-12-R and complementation tests indicated that HepI of the inner core of polysaccharide acts as the second receptor recognized by phage Bp7 and is essential for successful phage infection. This observation provides an explanation of the broad host range of phage Bp7 and provides insight into phage-host interactions. IMPORTANCE The RBPs of T4-like phages are gp37 and gp38. The interaction between phage T4 RBP gp37 and its receptors has been clarified by many reports. However, the interaction between gp38 and its receptors during phage adsorption is still not completely understood. Here, we identified phage Bp7, which uses gp38 as an RBP, and provided a good model to study the phage-host interaction mechanisms in an enterobacteriophage. Our study revealed that gp38 of phage Bp7 recognizes the outer membrane proteins (OMPs) LamB and OmpC of E. coli K-12 as specific receptors and binds with them reversibly. HepI of the inner-core oligosaccharide is the second receptor and binds with phage Bp7 irreversibly to begin the infection process. Determining the interaction between the phage and its receptors will help elucidate the mechanisms of phage with a broad host range and help increase understanding of the phage infection mechanism based on gp38.

Broad-host-range application of the srfA promoter from Bacillus subtilis in Escherichia coli

2020 ◽  
Vol 168 ◽  
pp. 105798 ◽  
Author(s):  
Chengran Guan ◽  
Yan Ma ◽  
Xuan Chen ◽  
Ruifeng Zhao ◽  
Xinyuan Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Host Range ◽  
Broad Host Range

scholarly journals Selection and Characterization of a Multivalent Salmonella Phage and Its Production in a Nonpathogenic Escherichia coli Strain

2010 ◽  
Vol 76 (21) ◽  
pp. 7338-7342 ◽  
Author(s):  
S. B. Santos ◽  
E. Fernandes ◽  
C. M. Carvalho ◽  
S. Sillankorva ◽  
V. N. Krylov ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Host Range ◽  
Biocontrol Agent ◽  
Coli Strain ◽  
Broad Host Range ◽  
Restriction Profile ◽  
Phage Dna ◽  
Dna Restriction ◽  
Salmonella Phage

ABSTRACT We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.

Sign in / Sign up

Export Citation Format

Share Document