scholarly journals Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Qing Liu ◽  
Zhao Liu ◽  
Changlin Zhang ◽  
Yanyan Xu ◽  
Xiaojing Li ◽  
...  

To determine the effect of 3% boric acid solution on cutaneous infections with Candida albicans (CA) in mice and its effect on skin microflora. Female mice were divided into three groups, with 18 mice in each group. Two injection sites were randomly selected, and 0.1 mL of CA mycelium suspension was injected into the epidermis and dermis of the back of mice. Group N was treated with sterile water for injection (SWFI). We observed the clinical manifestations, fungal fluorescence microscopic examination and colony count. Group B were hydropathically compressed with 3% boric acid solution for 30 min every 12 h. Group M was treated with SWFI, and group N was not treated. One week later, each group was observed with naked eyes, and skin samples were collected. The effect of boric acid on skin microflora was measured using Internal Transcribed Spacer Identification (ITS) and 16S rRNA genes. There were no significant changes in group M. In group B, the degree of skin injury was alleviated, the wounds healed markedly, and the exudate amount decreased. The effective rate of group B (83%) was significantly higher than that of group M (25%) (P < 0.05). The relative average abundance of Candida (P < 0.0001) and CA (P < 0.05) in group B was significantly lower than that in group M. Compared with group M, the microbial richness of group B changed little, but the diversity decreased. The flora structure of group B was significantly different from that of group M, but like that of group N. In group B, the abundance of Proteobacteria (P < 0.001), Enterobacteriaceae (P < 0.001), and Escherichia-Shigella (P < 0.001) was significantly greater, and the abundance of Firmicutes (P < 0.001), Staphylococcaceae (P < 0.001), and Staphylococcus (P < 0.001) were significantly lower. The 3% boric acid solution significantly reduced the symptoms of skin infection with Candida albicans. It inhibited the growth of Candida albicans and CA, reduced the diversity of skin microorganisms, increased the abundance of Proteobacteria, Enterobacteriaceae, Escherichia-Shigella, and reduced the abundance of Firmicutes, Staphylococcaceae, Staphylococcus.

Author(s):  
György Pátzay ◽  
József Dobor ◽  
Emil Csonka ◽  
Gábor Lozsi ◽  
Ferenc Feil

Borate ion exchange capacity of Purolite NRW600 strong base anion resin in hydroxide form and mixed bed NRW600+NRW100 ion exchange was investigated with static experiments. Anion exchange resin was saturated with 0.1–45 g/dm3 concentration boric acid solution in a static mixer at 20, 30, 40 and 50 °C at 150 rpm for 24 hours. Remaining borate content of saturation solutions was deter-mined with ion chromatography and ICP-OES. The amount of fixed borate as borate anions increased with the saturation borate concentration as well as in case of simple anion exchange as in case of mixed bed.Column sorption-elution study was carried out by using strong base anion exchange resins (Purolite NRW600 and Amberlite IRN78). Resins in hydroxide and in chloride forms were saturated in column with 5–40 g/dm3 boric acid solution in excess. The resin was then eluted with 200 cm3 salt free water with 5 cm3/min at 25 °C and then eluted by 1 mol/dm3 sodium-sulfate solution with 5 cm3/min. The effluent was collected and analyzed for borate content by titrimetric method. In chloride form the resin adsorbed and released much less borate. Effective borate and polyborate sorption needs hydroxide ions in resin phase.


2020 ◽  
Vol 405 ◽  
pp. 193-198
Author(s):  
Daniela Marušáková ◽  
Pavel Kůs ◽  
Lorant Szatmary ◽  
Iveta Adéla Prokůpková

We tested stability of 43 selective sorbents, covering the widest range of functional groups, efficiency of sorption, method of production, and which are theoretically useful for sorption of cesium, strontium, cobalt, arsenic and actinoids. We present 3 of them: A = TiO2, B = Al2O3 and C = MxH(TiO)4(SiO4)3 . z H2O. In first testing, sorbents were exposed to the model solution of boric acid for 168 hours, in which they were mixed in a closed container with a rotator. After the exposure, sorbent solutions were filtered and analysed by spectroscopic techniques (Infrared Spectroscopy (IR), Raman Spectroscopy (RS) and newly by Scanning Electron Microscopy (SEM), combined with Energy Dispersive Spectroscopy (EDS)). Exposed spectra were compared with spectra of new, non-exposed sorbents. In second testing, sorbents were exposed to gamma radiation for 12 days with total dose 666 ± 43 kGy, produced by cobalt bomb and analysed by same techniques. The aim of our testing was to find out how sorbents behave in model boric acid solution and what effect has the gamma radiation on sorbent spectra and to analyse sorbents by SEM with EDX.


1978 ◽  
Vol 61 (3) ◽  
pp. 720-726
Author(s):  
Ram B Roy ◽  
Anthony Buccafuri

Abstract An automated fluorometric procedure is described for assaying calcium pantothenate in multivitamin preparations. Sample extracts containing calcium pantothenate are treated on-line with a slurry of magnesium trisilicate which removes any interfering riboflavin that may be present. The nitrate is resampled, mixed online with a slurry of Dowex 50W-X4 (H+) which removes any interfering β-alanine that may be present, and dialyzed. Dialysates are hydrolyzed in an alkaline medium and reacted with a mixture of o-phthalaldehyde and 2-mercaptoethanoI in boric acid solution. The fluorescence intensity due to the formation of a fluorogenic compound is measured at 455 nm after excitation at 350 nm. The procedure developed is capable of analyzing 20 samples/hr. Analytical data indicate that calcium pantothenate is assayed reliably both from real and synthetic multivitamin preparations. The mean recovery of calcium pantothenate added to sample solutions of tablet composites was 95.4%.


2011 ◽  
Vol 77 (14) ◽  
pp. 5009-5017 ◽  
Author(s):  
Ilaria Pizzetti ◽  
Bernhard M. Fuchs ◽  
Gunnar Gerdts ◽  
Antje Wichels ◽  
Karen H. Wiltshire ◽  
...  

ABSTRACTMembers of the bacterial phylumPlanctomycetesare reported in marine water samples worldwide, but quantitative information is scarce. Here we investigated the phylogenetic diversity, abundance, and distribution ofPlanctomycetesin surface waters off the German North Sea island Helgoland during different seasons by 16S rRNA gene analysis and catalyzed reporter deposition fluorescencein situhybridization (CARD-FISH). GenerallyPlanctomycetesare more abundant in samples collected in summer and autumn than in samples collected in winter and spring. Statistical analysis revealed thatPlanctomycetesabundance was correlated to theCentralesdiatom bloom in spring 2007. The analysis of size-fractionated seawater samples and of macroaggregates showed that ∼90% of thePlanctomycetesreside in the >3-μm size fraction. Comparative sequence analysis of 184 almost full-length 16S rRNA genes revealed three dominant clades. The clades, namedPlanctomyces-related group A, unculturedPlanctomycetesgroup B, andPirellula-related group D, were monitored by CARD-FISH using newly developed oligonucleotide probes. All three clades showed recurrent abundance patterns during two annual sampling campaigns. UnculturedPlanctomycetesgroup B was most abundant in autumn samples, whilePlanctomyces-related group A was present in high numbers only during late autumn and winter. The levels ofPirellula-related group D were more constant throughout the year, with elevated counts in summer. Our analyses suggest that the seasonal succession of thePlanctomycetesis correlated with algal blooms. We hypothesize that the niche partitioning of the different clades might be caused by their algal substrates.


2014 ◽  
Vol 59 (12) ◽  
pp. 4200-4204 ◽  
Author(s):  
Maogang He ◽  
Chao Su ◽  
Xiangyang Liu ◽  
Xuetao Qi

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