scholarly journals Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes

2021 ◽  
Vol 12 ◽  
Author(s):  
Sara Gushgari-Doyle ◽  
Marcus Schicklberger ◽  
Yifan V. Li ◽  
Robert Walker ◽  
Romy Chakraborty
Keyword(s):  
Plant Growth ◽  
Panicum Virgatum ◽  
Growth Promotion ◽  
Pgp Traits ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Cultivation Methods ◽  
Diazotrophic Endophytes ◽  
Raoultella Terrigena

Endophytic nitrogen-fixing (diazotrophic) bacteria are essential members of the microbiome of switchgrass (Panicum virgatum), considered to be an important commodity crop in bioenergy production. While endophytic diazotrophs are known to provide fixed atmospheric nitrogen to their host plant, there are many other plant growth-promoting (PGP) capabilities of these organisms to be demonstrated. The diversity of PGP traits across different taxa of switchgrass-colonizing endophytes is understudied, yet critical for understanding endophytic function and improving cultivation methods of important commodity crops. Here, we present the isolation and characterization of three diazotrophic endophytes: Azospirillum agricola R1C, Klebsiella variicola F10Cl, and Raoultella terrigena R1Gly. Strains R1C and F10Cl were isolated from switchgrass and strain R1Gly, while isolated from tobacco, is demonstrated herein to colonize switchgrass. Each strain exhibited highly diverse genomic and phenotypic PGP capabilities. Strain F10Cl and R1Gly demonstrated the highest functional similarity, suggesting that, while endophyte community structure may vary widely based on host species, differences in functional diversity are not a clearly delineated. The results of this study advance our understanding of diazotrophic endophyte diversity, which will allow us to design robust strategies to improve cultivation methods of many economically important commodity crops.

Characterization of Root-Endophytic Actinobacteria From Cactus (Opuntia Ficus-Indica) for Plant Growth Promoting Traits

2021 ◽  
Author(s):  
V Govindasamy ◽  
Priya George ◽  
S V Ramesh ◽  
P Sureshkumar ◽  
Jagadish Rane ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Pgp Traits ◽  
Seedling Length ◽  
Plant Growth Promoting ◽  
Uninoculated Control ◽  
Growth Promoting ◽  
Endophytic Actinobacteria

Abstract The present study is the first report of isolation and characterization of endophytic actinobacteria from cactus (Opuntia ficus-indica). A total of 179 morphologically distinct endophytic actinobacterial isolates were purified from the roots of two different genetic accessions of cactus. All these isolates were screened for their plant growth promotion traits namely growth on N-free medium, P-solubilization, siderophore production, ACC deaminase activity and IAA production. A majority of the endophytic actinobacterial isolates (85%) exhibited the potential for plant growth promotion under in vitro conditions. Ten among the isolates were selected based on their multi-PGP traits and were identified as Streptomyces sp. based on the 16S rRNA gene sequencing and phylogenetic analysis. Plant growth promoting potential of these selected endophytic Streptomyces was studied in wheat seedlings. All these selected isolates significantly enhanced the growth parameters like seedling length and rootlets number compared to the uninoculated control. The wheat seeds inoculated with Streptomyces tuirus VL-70-IX exhibited maximum number of rootlets (6.33) compared to uninoculated control (3.67). The inoculation of endophytic actinobacteria Streptomyces pseudogriseolus VL-70-XII caused maximum seedling length (20.53 cm) and root length (8.26 cm) while the inoculation of S. radiopugnans HV-VIII resulted in highest shoot length (12.33 cm). These endophytic actinobacteria isolated from the roots of cactus accessions showed potential PGP traits. This work lays foundation for characterization and selection of endophytic actinobacteria from the under-exploited, drought tolerant species such as cactus with potential cross-compatibility for the improvement of plant growth of field crops especially under abiotic stress conditions..

scholarly journals Isolation and Characterization of Bacterial Endophytes from Lycopersicon esculentum Plant and their Plant Growth Promoting Characteristics

1970 ◽  
Vol 2 (1) ◽  
pp. 37-52 ◽  
Author(s):  
Hardik A Patel ◽  
Rajesh K Patel ◽  
Sunil M Khristi ◽  
Kruti Parikh ◽  
Geetha Rajendran
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Bacterial Endophytes ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Different Characteristics

The study was designed to isolate and characterize bacterial endophytes from root and stem of Lycopersicon esculentum plant which was collected form different region of Gujarat. Total 18 isolates of endophytic bacteria were selected in which, all the endophytic bacteria produced one or the other different characteristics involved in plant growth promotion. They either produced phytohormones like indole acetic acid, siderophore, protease, pectinase, organic acid showed antifungal activity, chromium tolerance and solubilized phosphate. Four of the strains among the 18 showed maximum positive results of plant growth promoting regulators (PGPR) test and among them best probable isolate was selected and thus its 16SrDNA was amplified and sequenced. Only HR7 endophyte of tomato turned out to be Pseudomonas aeruginosa. It’s a gram negative coccobacili, sporeforming motile bacilli and show maximum PGPR activity. The results of our present studies indicated that above strains might be endophytic and therefore, were associated with the plant growth. Keywords: Lycopersicon esculentum; endophytic bacteria; PGPR; IAA; 16SrDNA DOI: http://dx.doi.org/10.3126/njb.v2i1.5679   Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 37-52

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria Bacillus stratosphericus LW-03 from Lilium wardii

3 Biotech ◽  
2020 ◽  
Vol 10 (7) ◽  
Author(s):  
Mohammad Sayyar Khan ◽  
Junlian Gao ◽  
Mingfang Zhang ◽  
Xuqing Chen ◽  
The Su Moe ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting
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