scholarly journals Effect of Temperature and Cell Viability on Uranium Biomineralization by the Uranium Mine Isolate Penicillium simplicissimum

2021 ◽  
Vol 12 ◽  
Author(s):  
Sebastian Schaefer ◽  
Robin Steudtner ◽  
René Hübner ◽  
Evelyn Krawczyk-Bärsch ◽  
Mohamed L. Merroun

The remediation of heavy-metal-contaminated sites represents a serious environmental problem worldwide. Currently, cost- and time-intensive chemical treatments are usually performed. Bioremediation by heavy-metal-tolerant microorganisms is considered a more eco-friendly and comparatively cheap alternative. The fungus Penicillium simplicissimum KS1, isolated from the flooding water of a former uranium (U) mine in Germany, shows promising U bioremediation potential mainly through biomineralization. The adaption of P. simplicissimum KS1 to heavy-metal-contaminated sites is indicated by an increased U removal capacity of up to 550 mg U per g dry biomass, compared to the non-heavy-metal-exposed P. simplicissimum reference strain DSM 62867 (200 mg U per g dry biomass). In addition, the effect of temperature and cell viability of P. simplicissimum KS1 on U biomineralization was investigated. While viable cells at 30°C removed U mainly extracellularly via metabolism-dependent biomineralization, a decrease in temperature to 4°C or use of dead-autoclaved cells at 30°C revealed increased occurrence of passive biosorption and bioaccumulation, as confirmed by scanning transmission electron microscopy. The precipitated U species were assigned to uranyl phosphates with a structure similar to that of autunite, via cryo-time-resolved laser fluorescence spectroscopy. The major involvement of phosphates in U precipitation by P. simplicissimum KS1 was additionally supported by the observation of increased phosphatase activity for viable cells at 30°C. Furthermore, viable cells actively secreted small molecules, most likely phosphorylated amino acids, which interacted with U in the supernatant and were not detected in experiments with dead-autoclaved cells. Our study provides new insights into the influence of temperature and cell viability on U phosphate biomineralization by fungi, and furthermore highlight the potential use of P. simplicissimum KS1 particularly for U bioremediation purposes.Graphical Abstract

Author(s):  
Chahrazed Aibeche ◽  
Nawel Selami ◽  
Fatima El-Houaria Zitouni-Haouar ◽  
Khadidja Oeunzar ◽  
Amira Addou ◽  
...  

2009 ◽  
Vol 163 (1-4) ◽  
pp. 477-488 ◽  
Author(s):  
Grażyna A. Płaza ◽  
Grzegorz Nałęcz-Jawecki ◽  
Onruthai Pinyakong ◽  
Paul Illmer ◽  
Rosa Margesin

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Fusheng Zha ◽  
Dongdong Pan ◽  
Long Xu ◽  
Bo Kang ◽  
Chengbin Yang ◽  
...  

Solidification/stabilization (S/S) has been considered as one of the most effective techniques for remediation of the heavy metal-contaminated sites. Among various binders adopted in S/S, alkaline residue (AR) could be considered as a new binder to treat heavy metal-contaminated soil due to its strong adsorptive capacity for heavy metal ions. So in this paper, the strength, leaching, and microstructure characteristics of the solidified/stabilized Pb-contaminated soil by using alkaline residue are systematically investigated. Test results present that the unconfined compressive strength (UCS) of the treated soil will increase, while the leached Pb2+ concentration will decrease, with the increase of the alkaline residue content in the specimen. The UCS increases significantly with the curing time increasing during the initial 28 days, after which the UCS of the specimen becomes stable. The leached Pb2+ concentration decreases significantly at the initial 28 days followed by a stable trend with curing time increasing. The UCS decreases and the leached Pb2+ concentration increases with the increase of the initial Pb2+ concentration in the specimen. The microstructural analysis performed by scanning electron microscope (SEM) showed that the increase of the alkaline residue content and curing time will result in more hydration products and densified microstructure, which could effectively improve the engineering properties of the specimen.


2019 ◽  
Vol 37 ◽  
Author(s):  
M.J. KHAN ◽  
N. AHMED ◽  
W. HASSAN ◽  
T. SABA ◽  
S. KHAN ◽  
...  

ABSTRACT: Phytoremediation is a useful tool to restore heavy metals contaminated soils. This study was carried out to test two castor (Ricinus communis) cultivars [Local and DS-30] for phytoextraction of heavy metals from the soil spiked by known concentrations of seven metals (Cu, Cr, Fe, Mn, Ni, Pb and Zn). A pot experiment was laid out by using a completely randomized design. Soil and plant samples were analyzed at 100 days after planting. The data on heavy metal uptake by plant tissues (roots, leaves and shoots) of the two castor cultivars suggested that a considerable amount of metals (Fe = 27.18 mg L-1; Cu = 5.06 mg L-1; Cr = 2.95 mg L-1; Mn = 0.22 mg L-1; Ni = 4.66 mg L-1; Pb = 3.33 mg L-1; Zn = 15.04 mg L-1) was accumulated in the plant biomass. The soil heavy metal content at the end of experiment significantly decreased with both cultivars, resulting in improved soil quality. Therefore, it is concluded that both castor cultivars, Local and DS-30, can be used for phytoremediation of heavy metal-contaminated sites.


2022 ◽  
Author(s):  
Madhuri Girdhar ◽  
Zeba Tabassum ◽  
Kopal Singh ◽  
Anand Mohan

Heavy metals accumulated the earth crust and causes extreme pollution. Accumulation of rich concentrations of heavy metals in environments can cause various human diseases which risks health and high ecological issues. Mercury, arsenic, lead, silver, cadmium, chromium, etc. are some heavy metals harmful to organisms at even very low concentration. Heavy metal pollution is increasing day by day due to industrialization, urbanization, mining, volcanic eruptions, weathering of rocks, etc. Different microbial strains have developed very efficient and unique mechanisms for tolerating heavy metals in polluted sites with eco-friendly techniques. Heavy metals are group of metals with density more than 5 g/cm3. Microorganisms are generally present in contaminated sites of heavy metals and they develop new strategies which are metabolism dependent or independent to tackle with the adverse effects of heavy metals. Bacteria, Algae, Fungi, Cyanobacteria uses in bioremediation technique and acts a biosorbent. Removal of heavy metal from contaminated sites using microbial strains is cheaper alternative. Mostly species involved in bioremediation include Enterobacter and Pseudomonas species and some of bacillus species too in bacteria. Aspergillus and Penicillin species used in heavy metal resistance in fungi. Various species of the brown algae and Cyanobacteria shows resistance in algae.


2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Satya Prakash ◽  
Catherine Tomaro-Duchesneau ◽  
Shyamali Saha ◽  
Arielle Cantor

The gut microbiota plays a crucial role in maintaining health. Alterations of the gut bacterial population have been associated with a number of diseases. Past and recent studies suggest that one can positively modify the contents of the gut microbiota by introducing prebiotics, probiotics, synbiotics, and other therapeutics. This paper focuses on probiotic modulation of the gut microbiota by their delivery to the lower gastrointestinal tract (GIT). There are numerous obstacles to overcome before microorganisms can be utilized as therapeutics. One important limitation is the delivery of viable cells to the lower GIT without a significant loss of cell viability and metabolic features through the harsh conditions of the upper GIT. Microencapsulation has been shown to overcome this, with various types of microcapsules available for resolving this limitation. This paper discusses the gut microbiota and its role in disease, with a focus on microencapsulated probiotics and their potentials and limitations.


2007 ◽  
Vol 22 (5) ◽  
pp. 487-494 ◽  
Author(s):  
M. A. Q. Khan ◽  
S. A. Ahmed ◽  
A. Salazar ◽  
J. Gurumendi ◽  
A. Khan ◽  
...  

Author(s):  
IFA SULISTIYORINI ◽  
RATU SAFITRI ◽  
RONNY LESMANA ◽  
MAS RIZKY A. A. SYAMSUNARNO

Objective: In this study, the embryonic rat cardiomyocyte cell line H9C2 was used to investigate the cardiotoxicity effect of sappan wood ethanol extract (SWEE). Methods: Sappan wood was extracted in 96% ethanol and divided into dose concentrations of 2.5, 5, 10, 50, 100, and 150 μg/ml, with deferiprone used as a control. Cell viability was assessed using the PrestoBlue Cell Viability Reagent, according to manufacturer protocols. Results: Microscopic examination showed that the cell viability of H9C2 was preserved by SWEE treatments at a dose of 10 μg/ml and suggested dose concentrations of 50 μg/ml of SWEE. The percentage of viable cells was greater than 95% with a dose concentration of 10 μg/ml of SWEE, but it was significantly reduced with a dose concentration of 50 μg/ml of SWEE (p<0.05). Conclusion: The optimal dose concentration of SWEE to reach 95% cell viability was 10 μg/ml.


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