scholarly journals Biocontrol Potential of Aspergillus Species Producing Antimicrobial Metabolites

2021 ◽  
Vol 12 ◽  
Author(s):  
Men Thi Ngo ◽  
Minh Van Nguyen ◽  
Jae Woo Han ◽  
Bomin Kim ◽  
Yun Kyung Kim ◽  
...  

Microbial metabolites have been recognized as an important source for the discovery of new antifungal agents because of their diverse chemical structures with novel modes of action. In the course of our screening for new antifungal agents from microbes, we found that culture filtrates of two fungal species Aspergillus candidus SFC20200425-M11 and Aspergillus montenegroi SFC20200425-M27 have the potentials to reduce the development of fungal plant diseases such as tomato late blight and wheat leaf rust. From these two Aspergillus spp., we isolated a total of seven active compounds, including two new compounds (4 and 6), and identified their chemical structures based on the NMR spectral analyses: sphaeropsidin A (1), (R)-formosusin A (2), (R)-variotin (3), candidusin (4), asperlin (5), montenegrol (6), and protulactone A (7). Based on the results of the in vitro bioassays of 11 plant pathogenic fungi and bacteria, sphaeropsidin A (1), (R)-formosusin A (2), (R)-variotin (3), and asperlin (5) exhibited a wide range of antimicrobial activity. Furthermore, when plants were treated with sphaeropsidin A (1) and (R)-formosusin A (2) at a concentration of 500 μg/ml, sphaeropsidin A (1) exhibited an efficacy disease control value of 96 and 90% compared to non-treated control against tomato late blight and wheat leaf rust, and (R)-formosusin A (2) strongly reduced the development of tomato gray mold by 82%. Asperlin (5) at a concentration of 500 μg/ml effectively controlled the development of tomato late blight and wheat leaf rust with a disease control value of 95%. Given that culture filtrates and active compounds derived from two Aspergillus spp. exhibited disease control efficacies, our results suggest that the Aspergillus-produced antifungal compounds could be useful for the development of new natural fungicides.

2018 ◽  
Vol 28 (2) ◽  
pp. 262-266 ◽  
Author(s):  
Jae Woo Han ◽  
Sang Hee Shim ◽  
Kyoung Soo Jang ◽  
Yong Ho Choi ◽  
Hun Kim ◽  
...  

2008 ◽  
Vol 177 (4) ◽  
pp. 1001-1011 ◽  
Author(s):  
Christian Lannou ◽  
Samuel Soubeyrand ◽  
Lise Frezal ◽  
Joël Chadœuf
Keyword(s):  

1984 ◽  
Vol 64 (3) ◽  
pp. 511-519 ◽  
Author(s):  
G. L. C. MUSA ◽  
P. L. DYCK ◽  
D. J. SAMBORSKI

The inheritance of seedling resistance to isolate RLR 213/78 of rye leaf rust (Puccinia recondita f. sp. secalis) and race 30 of wheat leaf rust (P. recondita f. sp. tritici Rob.) was investigated in six inbred lines of rye (Secale cereale). Inbred line UM8116 was used as the susceptible parent in crosses. Inbred lines UM8003, UM8071 and UM8301 each have a single gene and UM8336 and UM8340 each have two genes for resistance to rye leaf rust. For resistance to wheat leaf rust UM8071 has a single gene, UM8003 and UM8340 each have two genes and UM8301 and UM8336 each have three genes. UM8295 is heterogeneous for reaction to both rusts. One of the genes in UM8340 may condition resistance to both rusts. The genes for resistance to RLR 213/78 appear to be independently inherited while some of the genes conferring resistance to race 30 may be identical or very closely linked. The potential of rye as a source of disease resistance for wheat and triticale improvement is discussed.Key words: Secale cereale, disease resistance, wheat leaf rust


2011 ◽  
Vol 79 (2) ◽  
pp. 180-188 ◽  
Author(s):  
Christoph Römer ◽  
Kathrin Bürling ◽  
Mauricio Hunsche ◽  
Till Rumpf ◽  
Georg Noga ◽  
...  

2013 ◽  
Vol 41 (2) ◽  
pp. 121-133
Author(s):  
Nour El-Din Soliman ◽  
Magdy Saber ◽  
Alaa Abd-Elaziz ◽  
Ibrahim Imbabi

2012 ◽  
pp. 33-62 ◽  
Author(s):  
B. McCallum ◽  
C. Hiebert ◽  
J. Huerta-Espino ◽  
S. Cloutier
Keyword(s):  

2012 ◽  
Vol 62 (1) ◽  
pp. 21-31 ◽  
Author(s):  
J. A. Kolmer ◽  
A. Hanzalova ◽  
H. Goyeau ◽  
R. Bayles ◽  
A. Morgounov

Genome ◽  
1998 ◽  
Vol 41 (6) ◽  
pp. 832-840 ◽  
Author(s):  
J Q Liu ◽  
J A Kolmer

An asexual field population and a sexually derived population of the wheat leaf rust fungus, Puccinia recondita, were examined and compared for diversity and linkage disequilibria between virulence and molecular phenotypes. Isolates in both populations were tested for virulence to 20 Thatcher wheat lines near-isogenic for leaf rust resistance genes, and for random amplified polymorphic DNA (RAPD) variation using 10 DNA primers. In the asexual field population, 36 virulence phenotypes and 14 RAPD phenotypes were identified in 43 isolates. In the sexual population, 87 virulence phenotypes and 79 RAPD phenotypes were identified in 104 isolates. Linkage disequilibria was less in the sexual population compared to the asexual field population. Virulence-RAPD phenotype pairs (110 in total) were directly compared between the two populations for association. In the asexual population, 39 virulence-RAPD phenotype pairs were associated (P < 0.05), compared with 18 pairs in the sexual population. Linkage was not evident, as some residual disequilibria remained between virulence and RAPD phenotypes. In the asexual population 18 RAPD phenotype pairs were associated, compared with 9 pairs in the sexual population. The sexual population was also tested for RAPD variation with an additional six primers. In the sexual population, amplification sites of four different primers were tightly linked which indicated a chromosomal segment in P. recondita may not readily undergo recombination. Disequilibria between virulence and RAPD phenotypes in field populations of P. recondita in Canada is maintained by asexual reproduction.Key words: Puccinia recondita, molecular diversity, virulence diversity, linkage disequilibria, wheat leaf rust.


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