scholarly journals Epigallocatechin Gallate (EGCG) Promotes the Immune Function of Ileum in High Fat Diet Fed Mice by Regulating Gut Microbiome Profiling and Immunoglobulin Production

2021 ◽  
Vol 8 ◽  
Author(s):  
Xiaoxia Liu ◽  
Ke Zhao ◽  
Nana Jing ◽  
Qingjun Kong ◽  
Xingbin Yang
Keyword(s):  
Gut Microbiome ◽  
High Fat Diet ◽  
Transmembrane Protein ◽  
Epigallocatechin Gallate ◽  
Intragastric Administration ◽  
High Fat ◽  
Immunoglobulin Production ◽  
Transcriptomic Profiling ◽  
Egcg Treatment ◽  
Microbiome Profiling

This study aimed to investigate the regulatory effect of epigallocatechin gallate (EGCG) on the composition of the gut microbiome, the transcriptomic profiling of ileum, and their interplay in high fat diet (HFD) induced obese mice. Intragastric administration of EGCG to C57BL/6J mice for 14 consecutive weeks remarkably decreased HFD induced excessive fat deposition (p < 0.001), and the increment of serum TG, TC, HDL-C (p < 0.05), as well as improved glucose tolerance (p < 0.001). EGCG shifted the gut microbiota mainly by elevating the relative abundance of Parasutterrlla, Bacteroides, and Akkermansia (p < 0.01), decreasing that of norank_f_Erysipelotrichaceae, unclassified_f_Ruminococcaceae, Anaerotruncus, Roseburia, norank_Lachnospiraceae, and Lachnospiraceae_UCG_006 (p < 0.01) at the genus level. In addition, EGCG affected the transcriptomic profiling of ileum, and the differentially expressed (DE) genes after HFD or/and EGCG treatment were mostly enriched in the immune reaction of ileum, such as the GO term of “immune effector process” and “phagocytosis, recognition.” Furthermore, the KEGG category of “immune diseases,” “immune system,” and “infection diseases: bacterial” were commonly enriched by the DE genes of the two treatments. Among those DE genes, 16 immunoglobulins heavy chain variable region encoded genes (Ighvs) and other immunity-related genes, such as complement component 2 (C2), interferon-induced transmembrane protein 1 (Iftm1), polymeric immunoglobulin receptor (pigR), and alanyl aminopeptidase (Anpep), were highly correlated with the shifted microbes in the gut (p < 0.05, absolute r > 0.5). Overall, the results suggested that EGCG ameliorated the HFD induced metabolic disorder mainly by regulating gut microbiome profiling and the immunoglobulin production of ileum, while the genes expressed in the ileum, especially Ighvs, C2, Iftm1, pigR, and Anpep, might play important roles in coordinating the immunity of mice regarding the gut microbes and the host interactions.

Gut microbiome alterations in high-fat-diet-fed mice are associated with antibiotic tolerance

2021 ◽  
Author(s):  
Yuan Liu ◽  
Kangni Yang ◽  
Yuqian Jia ◽  
Jingru Shi ◽  
Ziwen Tong ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
High Fat Diet ◽  
Antibiotic Tolerance ◽  
High Fat

scholarly journals Antibiotic perturbation of the murine gut microbiome enhances the adiposity, insulin resistance, and liver disease associated with high-fat diet

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Douglas Mahana ◽  
Chad M. Trent ◽  
Zachary D. Kurtz ◽  
Nicholas A. Bokulich ◽  
Thomas Battaglia ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Liver Disease ◽  
Gut Microbiome ◽  
High Fat Diet ◽  
High Fat

Seabuckthorn polysaccharide ameliorates high-fat diet-induced obesity by gut microbiota-SCFAs-liver axis

2022 ◽  
Author(s):  
Ying Lan ◽  
Qingyang Sun ◽  
Zhiyuan Ma ◽  
Jing Peng ◽  
Mengqi Zhang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Gut Microbiome ◽  
High Fat Diet ◽  
High Fat ◽  
Diet Induced Obesity

Obesity has been reported to be associated with gut microbiome dysbiosis. seabuckthorn fruits are traditionally used in Tibetan foods and medicines for thousands of years. Seabuckthorn polysaccharide (SP) is one...

Jamun ( Eugenia jambolana Lam.) Fruit Extract Prevents Obesity by Modulating the Gut Microbiome in High‐Fat‐Diet‐Fed Mice

2019 ◽  
Vol 63 (9) ◽  
pp. 1801307 ◽  
Author(s):  
Jialin Xu ◽  
Tingting Liu ◽  
Yuanyuan Li ◽  
Wei Liu ◽  
Zhanjun Ding ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
High Fat Diet ◽  
High Fat ◽  
Fruit Extract ◽  
Eugenia Jambolana

Gut Microbiome-Induced Shift of Acetate to Butyrate Positively Manages Dysbiosis in High Fat Diet

2018 ◽  
Vol 62 (3) ◽  
pp. 1700670 ◽  
Author(s):  
Xu Si ◽  
Wenting Shang ◽  
Zhongkai Zhou ◽  
Padraig Strappe ◽  
Bing Wang ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
High Fat Diet ◽  
High Fat

662 High Fat Diet Determines the Composition of the Gut Microbiome Independent of Host Genotype and Phenotype

2009 ◽  
Vol 136 (5) ◽  
pp. A-102 ◽  
Author(s):  
Marie A. Hildebrandt ◽  
Christian Hoffmann ◽  
Micah Hamady ◽  
Ying-Yu Chen ◽  
Rob Knight ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
High Fat Diet ◽  
High Fat ◽  
Host Genotype

scholarly journals PO-128 Effects of 30%, 50%, 70%VO2 max treadmill exercises on gut microbiome of atherosclerotic mice

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Wenting Shi ◽  
Jin Li ◽  
Pengyi Zhang
Keyword(s):  
Gut Microbiome ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
Exercise Group ◽  
High Fat ◽  
Vo2 Max ◽  
Adult Mice ◽  
Medium Intensity ◽  
Before And After ◽  
Low Intensity Exercise

Objective  To observe the dynamic changes of gut microbiome in mice before and after different intensities of treadmill exercises, and to explore the effects of different intensities of treadmill exercises on gut microbiome of atherosclerotic (AS) mice. Methods  The 50 male ApoE-/- mice aged 8 weeks were randomly divided into 2 groups, 10 mice in the general feeding group and the other 40 mice in the AS group, which were fed with normal and high-fat diet for 4 weeks respectively. Weight and blood test were taken before and after 4 weeks feeding. The serum Triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein (HDL) levels were examined by blood test in ApoE-/- mice model, and atherosclerotic disease degeneration score of adult mice was used to determine the success of modeling after 4 weeks feeding. Then two mice selected from the normal and high-fat group respectively selected to test the maximum oxygen uptake (VO2 max) by the reformative Bedford method, and the running platform speed and slope corresponding to 30%, 50% and 70%VO2 max were determined. The 40 AS mice were randomly divided into the model blank group, the low-intensity exercise group, the medium-intensity exercise group and the high-intensity exercise group according to their weight for 4 weeks of exercises, respectively. The low-intensity exercise group was 30% VO2 max, with a slope of 10 degrees and a speed of 10 m/min. The medium intensity exercise group was 50% VO2 max with a slope of 10 degrees and a speed of 15 m/min. The high-intensity exercise group was 70% VO2 max with a slope of 10 degrees and a speed of 20 m/min. The exercises were performed for 4 weeks, 5 days a week, 20 minutes a day. The fresh feces were collected from 5 groups of mice before and after 4 weeks treadmill exercises. The number of gut Lactobacillus, Bacteroides, Firmicutes, Bifidobacterium, Verrucomicrobiaceae, Akkermansia , Escherichia coli, Collinsella and Clostridium in AS mice were analyzed by 16s sequencing.  Results 1. The TG, TC and LDL-C were significantly increased in the blood serum of the mice after the modeling, and the HDL-C was decreased, and the atherosclerotic disease degeneration score was significantly increased in the adult mice, and the modeling was successful. 2. The different intensity treadmill exercises can increase the number of gut probiotics in mice and decrease the number of harmful bacteria. The beneficial bacteria in gut tract of AS mice with moderate intensity of 50% VO2max was observed in the experiment: Bacteroides, Bifidobacterium and Verrucomicrobiaceae, Akkermansia were significantly increased, P<0.05. The harmful bacteria:Lactobacillus, Escherichia coli, Collinsella and Clostridium were significantly decreased, P<0.05. The ratio of Firmicutes / Bacteroidetes was increased. Conclusions  1. High-fat diet can lead to AS in ApoE-/- mice. 2. The different intensity treadmill exercises can reduce the weight of AS mice. 3. The number and abundance of probiotics of gut microbiome of AS mice could be promoted by different intensity treadmill exercises, among which the medium intensity aerobic exercise can significantly increase the number of beneficial bacteria such Bacteroidetes, Bifidobacteria, Verrucomicrobiaceae and Akkermansia, meanwhile, the structure of gut microbiome in AS mice was improved as well.

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