scholarly journals In Vitro Red Blood Cell Segregation in Sickle Cell Anemia

2021 ◽  
Vol 9 ◽  
Author(s):  
Viviana Clavería ◽  
Philippe Connes ◽  
Luca Lanotte ◽  
Céline Renoux ◽  
Philippe Joly ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Flow Velocity ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Vascular Wall ◽  
Mean Flow ◽  
The Mean ◽  
The Impact ◽  
Mean Flow Velocity

Red blood cells in sickle cell anemia (sRBC) are more heterogeneous in their physical properties than healthy red blood cells, spanning adhesiveness, rigidity, density, size, and shape. sRBC with increased adhesiveness to the vascular wall would trigger vaso-occlusive like complications, a hallmark of sickle cell anemia. We investigated whether segregation occurs among sRBC flowing in micron-sized channels and tested the impact of aggregation on segregation. Two populations of sRBC of different densities were separated, labeled, and mixed again. The mixed suspension was flowed within glass capillary tubes at different pressure-drops, hematocrit, and suspending media that promoted or not cell aggregation. Observations were made at a fixed channel position. The mean flow velocity was obtained by using the cells as tracking particles, and the cell depleted layer (CDL) by measuring the distance from the cell core border to the channel wall. The labeled sRBC were identified by stopping the flow and scanning the cells within the channel section. The tube hematocrit was estimated from the number of fluorescence cells identified in the field of view. In non-aggregating media, our results showed a heterogeneous distribution of sRBC according to their density: low-density sRBC population remained closer to the center of the channel, while the densest cells segregated towards the walls. There was no impact of the mean flow velocity and little impact of hematocrit. This segregation heterogeneity could influence the ability of sRBC to adhere to the vascular wall and slow down blood flow. However, promoting aggregation inhibited segregation while CDL thickness was enhanced by aggregation, highlighting a potential protective role against vaso-occlusion in patients with sickle cell anemia.

Experimental Investigation of the Acoustic Reflection Coefficient of a Modeled Gas Turbine Impingement Cooling Section

2012 ◽  
Author(s):  
Christoph Jörg ◽  
Michael Wagner ◽  
Thomas Sattelmayer
Keyword(s):  
Reflection Coefficient ◽  
Flow Velocity ◽  
Gas Turbines ◽  
Acoustic Energy ◽  
Quality Data ◽  
Mean Flow ◽  
Impingement Cooling ◽  
Acoustic Reflection ◽  
The Mean ◽  
Mean Flow Velocity

The thermoacoustic stability of gas turbines depends on a balance of acoustic energy inside the engine. While the flames produce acoustic energy, other areas like the impingement cooling system contribute to damping. In this paper, we investigate the damping potential of an annular impingement sleeve geometry embedded into a realistic environment. A cold flow test rig was designed to represent real engine conditions in terms of geometry, and flow situation. High quality data was delivered by six piezoelectric dynamic pressure sensors. Experiments were carried out for different mean flow velocities through the cooling holes. The acoustic reflection coefficient of the impingement sleeve was evaluated at a downstream reference location. Further parameters investigated were the number of cooling holes, and the geometry of the chamber surrounding the impingement sleeve. Experimental results show that the determining parameter for the reflection coefficient is the mean flow velocity through the impingement holes. An increase of the mean flow velocity leads to significantly increased damping, and to low values of the reflection coefficient.

scholarly journals Elastic property of sickle cell anemia and sickle cell trait red blood cells

2021 ◽  
Vol 26 (09) ◽  
Author(s):  
Endris Muhammed ◽  
James Cooper ◽  
Daniel Devito ◽  
Robert Mushi ◽  
Maria del Pilar Aguinaga ◽  
...  
Keyword(s):  
Elastic Property ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Sickle Cell Trait

scholarly journals The effect of the antisickling compoundGBT1118 on the permeability of red blood cells from patients with sickle cell anemia

2019 ◽  
Vol 7 (6) ◽  
pp. e14027 ◽  
Author(s):  
Halima Al Balushi ◽  
Kobina Dufu ◽  
David C. Rees ◽  
John N. Brewin ◽  
Anke Hannemann ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells

scholarly journals Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease

2014 ◽  
Vol 6 (1) ◽  
pp. e2014066 ◽  
Author(s):  
Marco Marziali ◽  
Antonella Isgrò ◽  
Pietro Sodani ◽  
Javid Gaziev ◽  
Daniela Fraboni ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Marrow Transplant ◽  
Mixed Chimerism ◽  
Radical Cure ◽  
Hematopoietic Stem ◽  
Hematopoietic Chimerism

Allogeneic cellular gene therapy through hematopoietic stem cell transplantation is the only radical cure for congenital hemoglobinopathies like thalassemia and sickle cell anemia. Persistent mixed hematopoietic chimerism (PMC) has been described in thalassemia and sickle cell anemia. Here, we describe the clinical course of a 6-year-old girl who had received bone marrow transplant for sickle cell anemia. After the transplant, the patient showed 36% donor hematopoietic stem cells in the bone marrow, whereas in the peripheral blood there was evidence of 80%  circulating donor red blood cells (RBC). The analysis of apoptosis at the Bone Marrow  level suggests that Fas might contribute to the cell death of host erythroid precursors. The increase in NK cells and the regulatory T cell population observed in this patient suggests that these cells might contribute to the condition of mixed chimerism.

The nature of saltation and of ‘bed-load’ transport in water

1973 ◽  
Vol 332 (1591) ◽  
pp. 473-504 ◽  
Keyword(s):  
Flow Velocity ◽  
Bed Load ◽  
Flow Depth ◽  
Mean Flow ◽  
Stream Power ◽  
Fluid Turbulence ◽  
Load Transport ◽  
Bed Load Transport ◽  
The Mean ◽  
Mean Flow Velocity

Owing to observational difficulties the distinction between a ‘suspended’ load of solids transported by a stream and a ‘ bed-load ’ has long remained undefined. Recently, however, certain critical experiments have thrown much light on the nature of bed-load transport. In particular, it has been shown that bed-load transport, by saltation, occurs in the absence of fluid turbulence and must therefore be due to a separate dynamic process from that of transport in suspension by the internal eddy motion of a turbulent fluid. It has been further shown that the forward motion of saltating solids is opposed by a frictional force of the same order as the immersed weight of the solids, the friction coefficient approximating to that given by the angle of slip. The maintenance of steady motion therefore requires a predictable rate of energy dissipation by the transporting fluid. The fluid thrust necessary to maintain the motion is shown to be exerted by virtue of a mean slip velocity which is predictable in the same way as, and approxim ates to, the terminal fall velocity of the solid. The mean thrust, and therefore the transport rate of saltating solids, are therefore predictable in terms of the fluid velocity close to the bed, at a distance from it, within the saltation zone, of a ‘centre of fluid thrust’ analogous to the ‘centre of pressure’. This velocity, which is not directly measurable in water streams, can be got from a knowledge of stream depth and mean flow velocity. Thus a basic energy equation is obtained relating the rate of transporting work done to available fluid transporting power. This is shown to be applicable to the transport both of wind-blown sand, and of water-driven solids of all sizes and larger than that of medium sand. Though the mean flow velocity is itself unpredictable, the total stream power, which is the product of this quantity times the bed shear stress, is readily measurable. But since the mean flow velocity is an increasing function of flow depth, the transport of solids expressed in terms of total stream power must decrease with increasing flow depth/grain size ratio. This considerable variation with flow depth has not been previously recognised. It explains the gross inconsistencies found in the existing experimental data. The theoretical variation is shown to approximate very closely to that found in recent critical experiments in which transport rates were measured at different constant flow depths. The theory, which is largely confirmed by these and other earlier experiments, indicates that suspension by fluid turbulence of mineral solids larger than those of medium sands does not become appreciable until the bed shear stress is increased to a value exceeding 12 times its threshold value for the bed material considered. This range of unsuspended transport decreases rapidly, however, as the grain size is reduced till, at a certain critical size, suspension should occur at the threshold of bed movement.

scholarly journals Deep Learning Models for Classification of Red Blood Cells in Microscopy Images to Aid in Sickle Cell Anemia Diagnosis

Electronics ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 427 ◽  
Author(s):  
Laith Alzubaidi ◽  
Mohammed A. Fadhel ◽  
Omran Al-Shamma ◽  
Jinglan Zhang ◽  
Ye Duan
Keyword(s):  
Deep Learning ◽  
Red Blood Cells ◽  
Transfer Learning ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Learning Models ◽  
Sickle Cells ◽  
Microscopy Images

Sickle cell anemia, which is also called sickle cell disease (SCD), is a hematological disorder that causes occlusion in blood vessels, leading to hurtful episodes and even death. The key function of red blood cells (erythrocytes) is to supply all the parts of the human body with oxygen. Red blood cells (RBCs) form a crescent or sickle shape when sickle cell anemia affects them. This abnormal shape makes it difficult for sickle cells to move through the bloodstream, hence decreasing the oxygen flow. The precise classification of RBCs is the first step toward accurate diagnosis, which aids in evaluating the danger level of sickle cell anemia. The manual classification methods of erythrocytes require immense time, and it is possible that errors may be made throughout the classification stage. Traditional computer-aided techniques, which have been employed for erythrocyte classification, are based on handcrafted features techniques, and their performance relies on the selected features. They also are very sensitive to different sizes, colors, and complex shapes. However, microscopy images of erythrocytes are very complex in shape with different sizes. To this end, this research proposes lightweight deep learning models that classify the erythrocytes into three classes: circular (normal), elongated (sickle cells), and other blood content. These models are different in the number of layers and learnable filters. The available datasets of red blood cells with sickle cell disease are very small for training deep learning models. Therefore, addressing the lack of training data is the main aim of this paper. To tackle this issue and optimize the performance, the transfer learning technique is utilized. Transfer learning does not significantly affect performance on medical image tasks when the source domain is completely different from the target domain. In some cases, it can degrade the performance. Hence, we have applied the same domain transfer learning, unlike other methods that used the ImageNet dataset for transfer learning. To minimize the overfitting effect, we have utilized several data augmentation techniques. Our model obtained state-of-the-art performance and outperformed the latest methods by achieving an accuracy of 99.54% with our model and 99.98% with our model plus a multiclass SVM classifier on the erythrocytesIDB dataset and 98.87% on the collected dataset.

scholarly journals Three-dimensional spatial structure of the macro-pores and flow simulation in anthracite coal based on X-ray μ-CT scanning data

2020 ◽  
Vol 17 (5) ◽  
pp. 1221-1236
Author(s):  
Hui-Huang Fang ◽  
Shu-Xun Sang ◽  
Shi-Qi Liu
Keyword(s):  
Spatial Structure ◽  
Flow Velocity ◽  
Single Channel ◽  
Three Dimensional ◽  
Bedding Plane ◽  
Mean Flow ◽  
Equivalent Radius ◽  
Flow Pressure ◽  
The Mean ◽  
Mean Flow Velocity

Abstract The three-dimensional (3D) structures of pores directly affect the CH4 flow. Therefore, it is very important to analyze the 3D spatial structure of pores and to simulate the CH4 flow with the connected pores as the carrier. The result shows that the equivalent radius of pores and throats are 1–16 μm and 1.03–8.9 μm, respectively, and the throat length is 3.28–231.25 μm. The coordination number of pores concentrates around three, and the intersection point between the connectivity function and the X-axis is 3–4 μm, which indicate the macro-pores have good connectivity. During the single-channel flow, the pressure decreases along the direction of CH4 flow, and the flow velocity of CH4 decreases from the pore center to the wall. Under the dual-channel and the multi-channel flows, the pressure also decreases along the CH4 flow direction, while the velocity increases. The mean flow pressure gradually decreases with the increase of the distance from the inlet slice. The change of mean flow pressure is relatively stable in the direction horizontal to the bedding plane, while it is relatively large in the direction perpendicular to the bedding plane. The mean flow velocity in the direction horizontal to the bedding plane (Y-axis) is the largest, followed by that in the direction horizontal to the bedding plane (X-axis), and the mean flow velocity in the direction perpendicular to the bedding plane is the smallest.

scholarly journals Nocturnal enuresis and K+ transport in red blood cells from patients with sickle cell anemia

Haematologica ◽  
2016 ◽  
Vol 101 (12) ◽  
pp. e469-e472 ◽  
Author(s):  
S. Tewari ◽  
D. C. Rees ◽  
A. Hannemann ◽  
O. T. Gbotosho ◽  
H. W. M. Al Balushi ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Blood Cells ◽  
Nocturnal Enuresis ◽  
K Transport

Alteration of intraaneurysmal hemodynamics by placement of a self-expandable stent

2009 ◽  
Vol 111 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Satoshi Tateshima ◽  
Kazuo Tanishita ◽  
Yasuhiro Hakata ◽  
Shin-ya Tanoue ◽  
Fernando Viñuela
Keyword(s):  
Flow Velocity ◽  
Vortex Flow ◽  
Patient Specific ◽  
Mean Flow ◽  
Single Vortex ◽  
Clinical Images ◽  
The Mean ◽  
Fast Flow ◽  
Mean Flow Velocity ◽  
Flow Stream

Object Development of a flexible self-expanding stent system and stent-assisted coiling technique facilitates endovascular treatment of wide-necked brain aneurysms. The hemodynamic effect of self-expandable stent placement across the neck of a brain aneurysm has not been well documented in patient-specific aneurysm models. Methods Three patient-specific silicone aneurysm models based on clinical images were used in this study. Model 1 was constructed from a wide-necked internal carotid artery–ophthalmic artery aneurysm, and Models 2 and 3 were constructed from small wide-necked middle cerebral artery aneurysms. Neuroform stents were placed in the in vitro aneurysm models, and flow structures were compared before and after the stent placements. Flow velocity fields were acquired with particle imaging velocimetry. Results In Model 1, a clockwise, single-vortex flow pattern was observed in the aneurysm dome before stenting was performed. There were multiple vortices, and a very small fast flow stream was newly formed in the aneurysm dome after stenting. The mean intraaneurysmal flow velocity was reduced by ~ 23–40%. In Model 2, there was a clockwise vortex flow in the aneurysm dome and another small counterclockwise vortex in the tip of the aneurysm dome before stenting. The small vortex area disappeared after stenting, and the mean flow velocity in the aneurysm dome was reduced by 43–64%. In Model 3, a large, counterclockwise, single vortex was seen in the aneurysm dome before stenting. Multiple small vortices appeared in the aneurysm dome after stenting, and the mean flow velocity became slower by 22–51%. Conclusions The flexible self-expandable stents significantly altered flow velocity and also flow structure in these aneurysms. Overall flow alterations by the stent appeared favorable for the long-term durability of aneurysm embolization. The possibility that the placement of a low-profile self-expandable stent might induce unfavorable flow patterns such as a fast flow stream in the aneurysm dome cannot be excluded.

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