scholarly journals Methylsulfonylmethane Increases the Alveolar Bone Density of Mandibles in Aging Female Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Hanan Aljohani ◽  
Linda T. Senbanjo ◽  
Mohammed Al Qranei ◽  
Joseph P. Stains ◽  
Meenakshi A. Chellaiah
Keyword(s):  
Stem Cells ◽  
Bone Density ◽  
Bone Loss ◽  
Bone Formation ◽  
Trabecular Bone ◽  
Alveolar Bone ◽  
Specific Marker ◽  
Trabecular Bone Density ◽  
Female Mice

Methylsulfonylmethane (MSM) is a naturally occurring anti-inflammatory compound that effectively treats multiple degenerative diseases such as osteoarthritis and acute pancreatitis. Our previous studies have demonstrated the ability of MSM to differentiate stem cells from human exfoliated deciduous (SHED) teeth into osteoblast-like cells. This study examined the systemic effect of MSM in 36-week-old aging C57BL/6 female mice in vivo by injecting MSM for 13 weeks. Serum analyses showed an increase in expression levels of bone formation markers [osteocalcin (OCN) and procollagen type 1 intact N-terminal propeptide (P1NP)] and a reduction in bone resorption markers [tartrate-resistant acid phosphatase (TRAP) and C-terminal telopeptide of type I collag (CTX-I)] in MSM-injected animals. Micro-computed tomographic images demonstrated an increase in trabecular bone density in mandibles. The trabecular bone density tended to be higher in the femur, although the increase was not significantly different between the MSM- and phosphate-buffered saline (PBS)-injected mice. In mandibles, an increase in bone density with a corresponding decrease in the marrow cavity was observed in the MSM-injected mice. Furthermore, immunohistochemical analyses of the mandibles for the osteoblast-specific marker – OCN, and the mesenchymal stem cell-specific marker – CD105 showed a significant increase and decrease in OCN and CD105 positive cells, respectively. Areas of bone loss were observed in the inter-radicular region of mandibles in control mice. However, this loss was considerably decreased due to stimulation of bone formation in response to MSM injection. In conclusion, our study has demonstrated the ability of MSM to induce osteoblast formation and function in vivo, resulting in increased bone formation in the mandible. Hence, the application of MSM and stem cells of interest may be the right combination in alveolar bone regeneration under periodontal or other related diseases that demonstrate bone loss.

scholarly journals In Vivo Study for Clinical Application of Dental Stem Cell Therapy Incorporated with Dental Titanium Implants

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 381
Author(s):  
Hyunmin Choi ◽  
Kyu-Hyung Park ◽  
Narae Jung ◽  
June-Sung Shim ◽  
Hong-Seok Moon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Alveolar Bone ◽  
Human Mesenchymal Stem Cells ◽  
Titanium Implants ◽  
Osteogenic Potential ◽  
Induction Medium ◽  
Radiographic Analysis ◽  
New Bone Formation

The aim of this study was to investigate the behavior of dental-derived human mesenchymal stem cells (d-hMSCs) in response to differently surface-treated implants and to evaluate the effect of d-hMSCs on local osteogenesis around an implant in vivo. d-hMSCs derived from alveolar bone were established and cultured on machined, sandblasted and acid-etched (SLA)-treated titanium discs with and without osteogenic induction medium. Their morphological and osteogenic potential was assessed by scanning electron microscopy (SEM) and real-time polymerase chain reaction (RT-PCR) via mixing of 5 × 106 of d-hMSCs with 1 mL of Metrigel and 20 μL of gel-cell mixture, which was dispensed into the defect followed by the placement of customized mini-implants (machined, SLA-treated implants) in New Zealand white rabbits. Following healing periods of 2 weeks and 12 weeks, the obtained samples in each group were analyzed radiographically, histomorphometrically and immunohistochemically. The quantitative change in osteogenic differentiation of d-hMSCs was identified according to the type of surface treatment. Radiographic analysis revealed that an increase in new bone formation was statistically significant in the d-hMSCs group. Histomorphometric analysis was in accordance with radiographic analysis, showing the significantly increased new bone formation in the d-hMSCs group regardless of time of sacrifice. Human nuclei A was identified near the area where d-hMSCs were implanted but the level of expression was found to be decreased as time passed. Within the limitations of the present study, in this animal model, the transplantation of d-hMSCs enhanced the new bone formation around an implant and the survival and function of the stem cells was experimentally proven up to 12 weeks post-sacrifice.

Cortical and trabecular bone density measured in vivo by compton spectroscopy

Bone ◽  
1986 ◽  
Vol 7 (5) ◽  
pp. 394
Author(s):  
I. Leichter ◽  
A. Bivas ◽  
A. Giveon ◽  
J.Y. Margulies ◽  
A. Weinreb
Keyword(s):  
Bone Density ◽  
Trabecular Bone ◽  
Trabecular Bone Density

scholarly journals Alveolar bone trabeculae in patients with aggressive patients with aggressive periodontitis using cone beam computed tomography imaging

2012 ◽  
Vol 24 (2) ◽  
Author(s):  
Shaliha Shaliha ◽  
Ria Noerianingsih Firman ◽  
Yanti Rusyanti
Keyword(s):  
Computed Tomography ◽  
Bone Density ◽  
Trabecular Bone ◽  
Cone Beam Computed Tomography ◽  
Alveolar Bone ◽  
Aggressive Periodontitis ◽  
Cone Beam ◽  
Trabecular Bone Density ◽  
Computed Tomography Imaging ◽  
Tomography Imaging

Introduction: Periodontitis is an inflamatory process in supporting tissues of the teeth including the gingiva, cementum, periodontal ligament and alveolar bone. Perioditis ntitis begins with migration of junctional ephithelium toward the apical side and form a pocket on gingiva. Aggressive periodontitis is one of the classifications of periodontitis with characteristics of attacking teens to young adults in relatively fast can lead to severe alveolar bone loss and it is not comparable with local factors that there. Trabeculae are part of the alveolar bone covered by compact bone and cortical bone harder . On radiographs, trabeculae only seen as a radiolucent surrounded by a radiopaque. The purpose of this research was to know the description of alveolar trabecular bone in patients with aggressive periodontitis using Cone Beam Computed Tomography imaging. Methods: This study is descriptive on 72 samples of aggressive periodontitis alveolar travecular bon e by CBCT imaging, taken from 6 archival aggressive periodontitis patients as research subjects, obtained from secondary data in the Radiology Department of RSGM FKG UNPAD. Results: The results of this study from the 3D CBCT imaging of alveolar trabecular bone leading to further describe the more posterior a decline in trabecular bone density and decrease in patients with aggressive periodontitis. Conclusion: The 3D CBCT imaging of alveolar trabecular bone leading to further describe the more posterior a decline in trabecular bone density and decrease in patients with aggressive periodontitis.

Iliac trabecular bone formation predicts radial trabecular bone density changes in type 1 osteoporosis

2009 ◽  
Vol 6 (9) ◽  
pp. 929-935 ◽  
Author(s):  
Richard Hesp ◽  
Monique E. Arlot ◽  
Claude Edouard ◽  
Jeremy N. Bradbeer ◽  
Pierre J. Meunier ◽  
...  
Keyword(s):  
Bone Density ◽  
Bone Formation ◽  
Trabecular Bone ◽  
Trabecular Bone Density

scholarly journals Activity of 70% Ethanol Extract of Chrysophyllum cainito in Increasing Vertebrae Trabecular Bone Density in Female Mice

2018 ◽  
Vol 23 (3) ◽  
pp. 119 ◽  
Author(s):  
Nur Imamah Utaminingtyas ◽  
Burhan Ma'arif ◽  
Dewi Sinta Megawati ◽  
Ria Ramadhani Dwi Atmaja
Keyword(s):  
Bone Density ◽  
Trabecular Bone ◽  
Ethanol Extract ◽  
Positive Control ◽  
Optimum Dose ◽  
Trabecular Bone Density ◽  
Female Mice ◽  
Staining Methods ◽  
Chrysophyllum Cainito

Phytoestrogens are a class of compounds that have estrogen-like functions or structure. This research was conducted to find out the phytoestrogens activity in Chrysophyllum cainito leaves, in increasing the vertebrae trabecular bone density of female mice, using dexamethasone induction as osteoporosis model.  The 70% ethanol extract of C. cainito leaves was given to female mice with dose of 100; 200; and 400 mg/kgBW mice/day after being induced orally with dexamethasone with 0.145 mg/kgBW mice/day. The positive control used was 1.3 mg/kgBW mice/day alendronate. This research had been done in four weeks. The increasing of trabecular vertebrae bone density of female mice was measured with Histomorphometry staining methods. The result showed the 70% ethanol extract of C. cainito leaves have activity in increasing trabecular vertebrae bone density for female mice with optimum dose of 400 mg/kgBW mice/day. This activity occurs because phytoestrogens content in 70% ethanol extract of C. cainito leaves, that may replace the function of estrogen in its bond with ER.

Hemoglobin Levels and Bone Density in Older Persons: Results from the Inchianti Study.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1630-1630
Author(s):  
Matteo Cesari ◽  
Marco Pahor ◽  
Fulvio Lauretani ◽  
Brenda W.H.J. Penninx ◽  
Benedetta Bartali ◽  
...  
Keyword(s):  
Bone Density ◽  
Linear Regression ◽  
Bone Loss ◽  
Trabecular Bone ◽  
Cortical Bone ◽  
Trabecular Bone Density ◽  
Regression Analyses ◽  
Bone Area ◽  
Cortical Bone Density ◽  
Relationship Of

Abstract Background: Hypoxemia has been recognized as a risk factor for bone loss. The aim of the present study is to investigate the relationship of anemia and hemoglobin levels with bone mass and density measures in a large sample of older community-dwelling persons. Methods: The present study is based on data from 950 participants enrolled in the “Invecchiare in Chianti” (Aging in the Chianti area, InCHIANTI) study. All the analyses were performed considering continuous hemoglobin levels as well as the dichotomous anemia variable (defined according to WHO criteria as hemoglobin <12 g/dL in women and <13 g/dL in men). A peripheral quantitative computerized tomography (pQCT) scan of the right calf was performed in all participants to evaluate total bone density, trabecular bone density, cortical bone density, and the ratio between cortical and total bone area. Linear regression analyses were used to assess the multivariate relationship of pQCT bone measures with anemia and hemoglobin levels after adjustment for demographics, chronic conditions, muscle strength and biological variables. Results: Participants were 75.0 (SD 6.9) years old. In our sample, 101 participants (10.6%) were anemic. In women, coefficients from adjusted linear regression analyses evaluating the association between pQCT bone measures (per SD increase) and hemoglobin levels/anemia showed significant associations of anemia with total bone density (ß=−0.335, SE=0.163; p=0.04), and cortical bone density (ß=−0.428, SE=0.160; p=0.008). Relationships with borderline significance were found for the associations of anemia with trabecular bone density and the ratio between cortical and total bone area. Significant associations were found between hemoglobin levels and trabecular bone density (ß=0.112, SE=0.049; p=0.02), total bone density (ß=0.101, SE=0.046; p=0.03), cortical bone density (ß=0.100, SE=0.046; p=0.03), and the ratio between cortical bone and total area (ß=0.092, SE=0.045; p=0.04). In men, significant associations were found for the associations of hemoglobin levels with total bone density (ß=0.076, SE=0.036; p=0.03) and cortical bone density (ß=0.095, SE= 0.41; p=0.02). A borderline significance was reported for the association between anemia and cortical bone density. Conclusion: Anemia and low hemoglobin levels are negatively and independently associated with bone mass and density. The bone loss associated with hemoglobin levels mainly occurs in the cortical bone. Women with lower hemoglobin levels demonstrate a higher bone loss than men.

scholarly journals Peptidomimetic inhibitor of L-plastin reduces osteoclastic bone resorption in aging female mice

Bone Research ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Hanan Aljohani ◽  
Joseph P. Stains ◽  
Sunipa Majumdar ◽  
Deepa Srinivasan ◽  
Linda Senbanjo ◽  
...  
Keyword(s):  
Bone Density ◽  
Bone Resorption ◽  
Trabecular Bone ◽  
Formation Rate ◽  
Serum Levels ◽  
Mineral Apposition Rate ◽  
Trabecular Bone Density ◽  
Bone Formation Rate ◽  
Female Mice ◽  
Osteoclast Function

AbstractL-plastin (LPL) was identified as a potential regulator of the actin-bundling process involved in forming nascent sealing zones (NSZs), which are precursor zones for mature sealing zones. TAT-fused cell-penetrating small molecular weight LPL peptide (TAT- MARGSVSDEE, denoted as an inhibitory LPL peptide) attenuated the formation of NSZs and impaired bone resorption in vitro in osteoclasts. Also, the genetic deletion of LPL in mice demonstrated decreased eroded perimeters and increased trabecular bone density. In the present study, we hypothesized that targeting LPL with the inhibitory LPL peptide in vivo could reduce osteoclast function and increase bone density in a mice model of low bone mass. We injected aging C57BL/6 female mice (36 weeks old) subcutaneously with the inhibitory and scrambled peptides of LPL for 14 weeks. Micro-CT and histomorphometry analyses demonstrated an increase in trabecular bone density of femoral and tibial bones with no change in cortical thickness in mice injected with the inhibitory LPL peptide. A reduction in the serum levels of CTX-1 peptide suggests that the increase in bone density is associated with a decrease in osteoclast function. No changes in bone formation rate and mineral apposition rate, and the serum levels of P1NP indicate that the inhibitory LPL peptide does not affect osteoblast function. Our study shows that the inhibitory LPL peptide can block osteoclast function without impairing the function of osteoblasts. LPL peptide could be developed as a prospective therapeutic agent to treat osteoporosis.

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