scholarly journals Smooth Endoplasmic Reticulum Clusters in Oocytes From Patients Who Received Intracytoplasmic Sperm Injections Negatively Affect Blastocyst Quality and Speed of Blastocyst Development

2021 ◽  
Vol 12 ◽  
Author(s):  
Xue Wang ◽  
YaLing Xiao ◽  
ZhengYi Sun ◽  
JingRan Zhen ◽  
Qi Yu
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Multiple Logistic Regression Analysis ◽  
Fertilization Rate ◽  
Multiple Logistic Regression ◽  
Blastocyst Development ◽  
Significant Difference ◽  
Blastocyst Quality ◽  
Blastocyst Rate ◽  
The Relationship

Findings regarding the relationship between smooth endoplasmic reticulum clusters (SERCs) in oocytes and blastocyst development have been conflicting. In this study, the effects of SERCs on blastocyst quality and the speed of blastocyst development were evaluated. Patients who received intracytoplasmic sperm injections (ICSI) at our reproductive center from 2016 to 2020 were retrospectively analyzed. SERC (+) oocytes (n = 217) and SERC (–) oocytes (n = 822), as well as SERC (+) cycles (n = 146) and SERC (–) cycles (n = 1,951) were compared. There was no significant difference in embryological, clinical, and neonatal outcomes between the SERC (+) and SERC (–) cycles. The fertilization rate (73.9%), good quality blastocyst rate (26.7%) and the speed of blastocyst development (44.4%) were significantly lower (P < 0.05) in SERC (+) oocytes than in unaffected counterparts (86.2%, 44.1% and 63.4%, respectively). Furthermore, the proportion of blastocysts with trophectoderm (TE) grade C was significantly higher in the SERC (+) oocyte group than in the SERC (–) oocyte group (73.3 vs. 55.9%, P < 0.05). After adjusting for age, years of infertility, endometriosis, stimulation protocols (GnRHa), and male infertility, multiple logistic regression analysis revealed that the presence of SERCs in the oocytes significantly affected the speed of blastocyst development (odds ratio, 2.812; 95% CI, 1.257–6.292; P = 0.012). These findings suggest that the presence of SERCs in oocytes may negatively affect blastocyst quality and the speed of blastocyst development.

scholarly journals Oocytes with smooth endoplasmic reticulum aggregates do not impact blastocyst euploidy rate

2021 ◽  
Author(s):  
Jian Xu ◽  
Li Yang ◽  
Zhi-Heng Chen ◽  
Min-Na Yin ◽  
Juan Chen ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Formation Rate ◽  
Tertiary Hospital ◽  
Fertilization Rate ◽  
Blastocyst Formation ◽  
Preimplantation Genetic Testing ◽  
Significant Difference ◽  
Normal Fertilization ◽  
Euploidy Rate

Abstract Objective: To investigate whether the euploidy rate of blastocysts derived from smooth endoplasmic reticulum (SERa) positive cycles and oocytes are impacted.Design: Retrospective cohort study.Setting: A tertiary hospital-based reproductive medicine center.Patient(s): A total of 601 preimplantation genetic testing (PGT) cycles with obtained oocytes in our center between April 2017 and May 2021 were included in the study. Intervention(s): Women＞35 years and PGT cycles with chromosomal structural rearrangements (PGT-SR) were excluded. Embryological and blastocyst ploidy outcomes were compared between SERa+ oocyte, sibling SERa- oocytes and oocytes in SERa- cycles.Main Outcome Measure(s): Embryological outcomes and blastocyst euploidy rate.Results: No significant difference was observed in the normal fertilization rate (82.1 % vs. 77.8 % vs. 83.1 %, respectively, P=0.061), blastocyst formation rate (71.0 % vs. 72.5 % vs. 68.4 %, respectively, P=0.393), good quality blastocyst formation rate (46.4 % vs. 48.3 % vs. 42.6 %, respectively, P=0.198) between the SERa+ oocyte group, sibling SERa- oocyte group and SERa- oocyte group. No significant difference was observed in the euploidy rate (50.0 % vs. 62.5 % vs. 63.3 %, respectively, P=0.324), mosaic rate (12.5 % vs. 9.7 % vs. 13.4 %, respectively, P=0.506) and aneuploidy rate (37.5 % vs. 27.8% vs. 23.2 %, respectively, P=0.137) between the three groups.Conclusion: Our results suggest that the euploidy rate of blastocysts derived from SERa+ cycles and oocytes are not impacted.

P–570 Embryos originating from oocytes with smooth endoplasmic reticulum clusters have a lower euploidy rate via PGT-A testing using next-generation sequencing

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
C J Li ◽  
C L Chang ◽  
H Y Huang ◽  
Y K Soong ◽  
H M Wu
Keyword(s):  
Endoplasmic Reticulum ◽  
Large Scale ◽  
Smooth Endoplasmic Reticulum ◽  
Polar Body ◽  
Live Birth Rate ◽  
Fertilization Rate ◽  
Chang Gung Memorial Hospital ◽  
Preimplantation Genetic Testing ◽  
Blastocyst Rate ◽  
Euploidy Rate

Abstract Study question Does the presence of smooth endoplasmic reticulum clusters (sERCs) in oocytes affect the human embryo ploidy? Summary answer The euploidy rate of embryos originating from sERCs + oocytes is lower What is known already While an expert panel strongly recommended that sERCs+ oocytes should not be inseminated, some normal healthy babies derived from sERCs+ oocytes have been reported. In previous studies have shown that declined fertilization rate and lower proportions of good quality embryos are found in oocytes showing sERCs. The updating findings of the molecular status of sERC+ oocytes elucidated the sERCs+ oocytes may have impaired chromosomal segregation ability. However, no study reveals the relation between sERCs and embryo ploidy. Study design, size, duration A retrospective study enrolled 129 preimplantation genetic testing (PGT) cycles from January 2017 to March 2020 at Chang Gung Memorial Hospital, Lonkou. Participants/materials, setting, methods ICSI fertilization rate, Day5 usable blastocyst rate (D5UBR), total usable blastocyst rate (TUBR), euploidy rate, mosaic rate, and aneuploidy rate are investigated between embryo originating from sERCs+ and sERCs- oocytes. Main results and the role of chance Although higher TBUR in blastocyst derived from sERCs+ oocytes than sERCs- group (73.7% vs. 62.5%) but accompanied lower euploidy rate (7% vs. 29%) and higher aneuploid rate (79% vs. 54%). Limitations, reasons for caution Limited sample size, need a large-scale study to confirm the conclusion. The live-birth rate per embryo transfer cycle was not included for analysis. As we did not perform polar body analysis, we cannot state for sure that embryonic aneuploidy was related to the oocyte. Wider implications of the findings: This study demonstrates that embryos originating from sERCs+ oocytes have a lower euploidy rate. Trial registration number CMRPG3H0751

Interaction of the smooth endoplasmic reticulum and mitochondria

2006 ◽  
Vol 34 (3) ◽  
pp. 370-373 ◽  
Author(s):  
J.G. Goetz ◽  
I.R. Nabi
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Close Association ◽  
Cytosolic Calcium ◽  
Functional Roles ◽  
Calcium Dependent ◽  
Motility Factor ◽  
Calcium Sequestration ◽  
Multiple Domains ◽  
The Relationship

The ER (endoplasmic reticulum) is composed of multiple domains including the nuclear envelope, ribosome-studded rough ER and the SER (smooth ER). The SER can also be functionally segregated into domains that regulate ER–Golgi traffic (transitional ER), ERAD (ER-associated degradation), sterol and lipid biosynthesis and calcium sequestration. The last two, as well as apoptosis, are critically regulated by the close association of the SER with mitochondria. Studies with AMFR (autocrine motility factor receptor) have defined an SER domain whose integrity and mitochondrial association can be modulated by ilimaquinone as well as by free cytosolic calcium levels in the normal physiological range. AMFR is an E3 ubiquitin ligase that targets its ligand directly to the SER via a caveolae/raft-dependent pathway. In the present review, we will address the relationship between the calcium-dependent morphology and mitochondrial association of the SER and its various functional roles in the cell.

Prognostic Nomogram on Clinicopathologic Features and Serum Uric Acid for Precancerous Lesions and Gastric Cancer

2021 ◽  
Author(s):  
Shanshan Tang ◽  
Ying Chen ◽  
Chenhong Fu ◽  
Xin Xie ◽  
Ziyu Song ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Uric Acid ◽  
Serum Uric Acid ◽  
Precancerous Lesions ◽  
Multiple Logistic Regression Analysis ◽  
Precancerous Lesion ◽  
Multiple Logistic Regression ◽  
Diagnosis Rate ◽  
H Pylori ◽  
The Relationship

Abstract The relationship between Uric acid (UA) and malignant tumor are still confusing. Gastric cancer(GC) is recognized to be closely related to Helicobacter pylori (H. pylori) infection, early diagnosis rate is very low. In this study, we aimed to investigate the relationship between H. pylori and hyperuricemia (HUA), and evaluate the predictive value of serum uric acid (SUA) in gastric precancerous lesion (GPL) and gastric cancer (GC). This retrospective study included 486 patients who underwent gastroscopy (155 controls, 272 GPL, 59 GC patients). The risk factors for GPL and GC were identified by multiple logistic regression analysis and nomogram was constructed to evaluate the ability of SUA to predict the risk of these diseases based on SUA score. We found that in healthy controls, HUA is positively correlated with H. Pylori (+). SUA was an independent risk factor for GPL and GC. Verification shows that the nomogram was better fitted for GC than for GPL. In conclusion, our study established nomogram based on SUA to predict the risk of GPL and GC, suggested that the incidence of GPL and GC is higher in H. pylori (+) HUA patients, so early intervention and vigilance should be raised.

scholarly journals C-MAC Video Laryngoscope versus Conventional Direct Laryngoscopy for Endotracheal Intubation During Cardiopulmonary Resuscitation

Medicina ◽  
2019 ◽  
Vol 55 (6) ◽  
pp. 225 ◽  
Author(s):  
Byeong Chul Min ◽  
Jong Eun Park ◽  
Gun Tak Lee ◽  
Tae Rim Kim ◽  
Hee Yoon ◽  
...  
Keyword(s):  
Emergency Department ◽  
Logistic Regression ◽  
Cardiopulmonary Resuscitation ◽  
Endotracheal Intubation ◽  
Direct Laryngoscopy ◽  
Multiple Logistic Regression Analysis ◽  
Video Laryngoscope ◽  
Multiple Logistic Regression ◽  
Total N ◽  
Significant Difference

Background and objectives: To compare the first pass success (FPS) rate of the C-MAC video laryngoscope (C-MAC) and conventional Macintosh-type direct laryngoscopy (DL) during cardiopulmonary resuscitation (CPR) in the emergency department (ED). Materials and Methods: This study was a single-center, retrospective study conducted from April 2014 to July 2018. Patients were categorized into either the C-MAC or DL group, according to the device used on the first endotracheal intubation (ETI) attempt. The primary outcome was the FPS rate. A multiple logistic regression model was developed to identify factors related to the FPS. Results: A total of 573 ETIs were performed. Of the eligible cases, 263 and 310 patients were assigned to the C-MAC and DL group, respectively. The overall FPS rate was 75% (n = 431/573). The FPS rate was higher in the C-MAC group than in the DL group, but there was no statistically significant difference (total n = 431, 79% compared to 72%, p = 0.075). In the multiple logistic regression analysis, the C-MAC use had higher FPS rate (adjusted odds ratio: 1.80; 95% CI, 1.17–2.77; p = 0.007) than that of the DL use. Conclusions: The C-MAC use on the first ETI attempt during cardiopulmonary resuscitation in the emergency department had a higher FPS rate than that of the DL use.

scholarly journals The distribution of the components of mixed-function oxidase between the rough and the smooth endoplasmic reticulum of liver cells

1968 ◽  
Vol 110 (3) ◽  
pp. 407-412 ◽  
Author(s):  
J. L. Holtzman ◽  
T. E. Gram ◽  
P. L. Gigon ◽  
J. R. Gillette
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Reductase Activity ◽  
Mixed Function Oxidase ◽  
Protein Ratio ◽  
Nadph Cytochrome ◽  
Rate Limiting Step ◽  
Significant Difference ◽  
The Difference ◽  
Cytochrome P 450

Mixed-function oxidase activity, when measured by the N-demethylation of ethylmorphine or the hydroxylation of aniline, is significantly higher in the smooth hepatic endoplasmic reticulum than in the rough. In the rabbit the smooth membrane/rough membrane activity ratios are significantly greater than 1 whether the activities are expressed per g. of liver (ratio 5), per mg. of protein (ratio 3–5), per μg. of phospholipid phosphorus (ratio 2), per unit of cytochrome P-450 (ratio 1·7) or per unit of NADPH–cytochrome c reductase activity (ratio 2). On the other hand, if the activities are normalized to the NADPH–cytochrome P-450 reductase, there is no significant difference between the rough and smooth membranes. These results suggest that, in the rabbit, the rate-limiting step is the reduction of cytochrome P-450. In contrast, in the rat the difference in activities can be explained by differences in the concentration of cytochrome P-450.

Sleep habits and psychosomatic health complaints of bank workers in a megacity in Japan

1995 ◽  
Vol 27 (4) ◽  
pp. 467-472 ◽  
Author(s):  
Yutaka Motohashi ◽  
Takehito Takano
Keyword(s):  
Mental Stress ◽  
Sleep Disturbances ◽  
Multiple Logistic Regression Analysis ◽  
Depressive Mood ◽  
Health Complaints ◽  
Multiple Logistic Regression ◽  
Sleep Habits ◽  
Psychosomatic Health ◽  
The Relationship ◽  
Rising Time

SummaryThe relationship between sleep habits and psychosomatic health complaints was investigated by a questionnaire study in 148 bank workers in Tokyo. Daily bedtime was more flexible than daily rising time, and both mean bedtime and rising time tended to be earlier with increasing age. Multiple logistic regression analysis showed that psychosomatic health complaints of sleep disturbances, fatigue, digestive troubles, and depressive mood were significantly associated with sleep habits and perceived mental stress.

scholarly journals REPRODUCTIVE PERFORMANCE OF DOMESTICATED BROODSTOCK OF SILVER PERCH, Bidyanus bidyanus (MITCHELL 1838) AND THE RELATIONSHIP BETWEEN OIL GLOBULE FRAGMENTATION AND EGG QUALITY

2017 ◽  
Vol 12 (2) ◽  
pp. 43 ◽  
Author(s):  
Sulaeman Sulaeman ◽  
Ravi Fotedar
Keyword(s):  
Survival Rate ◽  
Reproductive Performance ◽  
Egg Quality ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
High Fecundity ◽  
Fertilisation Rate ◽  
Significant Difference ◽  
The Relationship

The experiments investigated the reproductive performance of the domesticated broodstock of the silver perch and the relationship between various degrees of oil globule fragmentation and egg quality. Six years old of second generation broodstock (n=3) were evaluated based on the fecundity, fertilisation rate, hatching rate, the degree of oil fragmentation of egg, and the quality of embryos and larvae produced. The fragmentation were grouped into three categories: un-fragmented (cat-1), moderately fragmented (cat-2), and highly fragmented (cat-3). The results showed that the broodstock had a relatively high fecundity (132,400 ± 7,22), fertilization rate (94.27 ± 1.28%), and hatching rates (87.94 ± 1.23%). The survival rate of larvae at 12 days post hatching (dph) in cat-1 (71.3 ± 0.9%) was higher than cat-2 (66.7 ± 0.9%) whereas cat-2 was higher than cat-3 (61.3 ± 0.3%). The eggs was dominated by cat-1 (78.11 ± 2.44%) which was significantly higher than cat-2 (21.26 ± 2.45%) and cat-3 ones (0.40 ± 0.21%). The survival rate of embryo at 20 hours post spawning (hps) and hatching rate of cat-1 (95.33 ± 0.00% and 93.33 ± 0.00%) and cat-2 (90.00 ± 0.00% and 85.00 ± 0.00%) were significantly higher than cat-3 (72.33 ± 1.76% and 60.33 ± 0.00%). The total length (TL) of the larvae of cat-1 and cat-2 (8.44 ± 0.21 mm and 8.35 ± 0.23 mm respectively) were significantly higher than larvae of cat-3 (7.09 ± 0.14 mm). No significant difference was found in the larval deformities among any categories. In conclusion, the reproductive performance of six year-old broodstock silver perch showing acceptable performance and egg categorisation based on oil globule fragmentation can be used as a useful tool to indicate eggs quality of silver perch.

scholarly journals Association of MC4R (rs17782313) with diabetes and cardiovascular disease in Korean men and women

2020 ◽  
Author(s):  
Jae Woong Sull ◽  
Gitae Kim ◽  
Sun Ha Jee
Keyword(s):  
Cardiovascular Disease ◽  
Regression Models ◽  
Fasting Glucose ◽  
Multiple Logistic Regression Analysis ◽  
Multiple Logistic Regression ◽  
Fasting Glucose Level ◽  
Linear Regression Models ◽  
Glucose Levels ◽  
Mc4r Rs17782313 ◽  
The Relationship

Abstract Background: Diabetes is mostly assessed by the fasting glucose level. Several studies reported that serum fasting glucose levels and cardiovascular disease are associated with MC4R. Methods: A total of 4,294 subjects participated in this study. There were 1810 subjects with cardiovascular disease among the 4294 subjects. We used multivariate linear regression models and multiple logistic regression analysis. Results: Individuals with the TC/CC genotype had a 1.29-fold higher risk of diabetes than did those with the TT genotype when adjusting for age, sex, and BMI (OR, 1.29; 95% CI, 1.04-1.60). For healthy subjects, the association was significant in women (OR, 1.99; 95% CI, 1.01-3.93). Men with the TC/CC genotype had a 1.21-fold higher risk of cardiovascular disease than did those with the TT genotype when adjusting for age, sex, and BMI (OR, 1.21; 95% CI, 1.04-1.41). The relationship between MC4R and cardiovascular disease was stronger in lean men (OR, 1.40; 95% CI, 1.12–1.74, p = 0.0028) than in overweight men.Conclusions: This study suggests that the rs17782313 SNP in MC4R is related to diabetes and the SNP is also associated with cardiovascular disease in lean men.

scholarly journals Oocytes With Smooth Endoplasmic Reticulum Aggregates Are Not Associated With Impaired Reproductive Outcomes: A Matched Retrospective Cohort Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Jian Xu ◽  
Li Yang ◽  
Zhi-Heng Chen ◽  
Min-Na Yin ◽  
Juan Chen ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Embryo Transfer ◽  
Live Birth ◽  
Birth Defects ◽  
Birth Rate ◽  
Smooth Endoplasmic Reticulum ◽  
Live Birth Rate ◽  
Clinical Pregnancy ◽  
Reproductive Outcomes ◽  
Significant Difference

ObjectiveTo investigate whether the reproductive outcomes of oocytes with smooth endoplasmic reticulum aggregates (SERa) are impaired.MethodsA total of 2893 intracytoplasmic sperm injection (ICSI) cycles were performed between January 2010 and December 2019 in our center. In 43 transfer cycles, transferred embryos were totally derived from SERa+ oocytes. Each of the 43 cycles was matched with a separate control subject from SERa- patient of the same age ( ± 1 year), embryo condition, main causes of infertility, type of protocols used for fresh or frozen embryo transfer cycles. The clinical pregnancy, implantation, ectopic pregnancy and live birth rate were compared between the two groups.Results43 embryo transfer cycles from SERa- patient were matched to the 43 transferred cycles with pure SERa+ oocytes derived embryos. No significant difference was observed in clinical pregnancy rate (55.81% vs. 65.11%, p=0.5081), implantation rate (47.89% vs. 50.70%, p=0.8667) and live birth rate (48.84% vs. 55.81%, p=0.6659) between the SERa+ oocyte group and the matched group. No congenital birth defects were found in the two groups.ConclusionOur results suggest that the implantation, clinical pregnancy, live birth and birth defects rate of embryos derived from oocytes with SERa are not impaired.

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