Genetic Analysis of Heterosis for Yield Influencing Traits in Brassica juncea Using a Doubled Haploid Population and Its Backcross Progenies

The exploitation of heterosis through hybrid breeding is one of the major breeding objectives for productivity increase in crop plants. This research analyzes the genetic basis of heterosis in Brassica juncea by using a doubled haploid (DH) mapping population derived from F1 between two heterotic inbred parents, one belonging to the Indian and the other belonging to the east European gene pool, and their two corresponding sets of backcross hybrids. An Illumina Infinium Brassica 90K SNP array-based genetic map was used to identify yield influencing quantitative trait loci (QTL) related to plant architecture, flowering, and silique- and seed-related traits using five different data sets from multiple trials, allowing the estimation of additive and dominance effects, as well as digenic epistatic interactions. In total, 695 additive QTL were detected for the 14 traits in the three trials using five data sets, with overdominance observed to be the predominant type of effect in determining the expression of heterotic QTL. The results indicated that the design in the present study was efficient for identifying common QTL across multiple trials and populations, which constitute a valuable resource for marker-assisted selection and further research. In addition, a total of 637 epistatic loci were identified, and it was concluded that epistasis among loci without detectable main effects plays an important role in controlling heterosis in yield of B. juncea.

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Novel QTL Associated with Shoot Branching Identified in Doubled Haploid Rice (Oryza sativa L.) under Low Nitrogen Cultivation

Genes ◽

10.3390/genes12050745 ◽

2021 ◽

Vol 12 (5) ◽

pp. 745

Author(s):

Youngho Kwon ◽

Nkulu Rolly Kabange ◽

Ji-Yun Lee ◽

So-Myeong Lee ◽

Jin-Kyung Cha ◽

...

Keyword(s):

Doubled Haploid ◽

Phenotypic Variation ◽

Growth And Development ◽

Oryza Sativa L ◽

Tiller Number ◽

Yield Potential ◽

Doubled Haploid Population ◽

Shoot Branching ◽

Normal Nitrogen ◽

Low Nitrogen

Shoot branching is considered as an important trait for the architecture of plants and contributes to their growth and productivity. In cereal crops, such as rice, shoot branching is controlled by many factors, including phytohormones signaling networks, operating either in synergy or antagonizing each other. In rice, shoot branching indicates the ability to produce more tillers that are essential for achieving high productivity and yield potential. In the present study, we evaluated the growth and development, and yield components of a doubled haploid population derived from a cross between 93-11 (P1, indica) and Milyang352 (P2, japonica), grown under normal nitrogen and low nitrogen cultivation open field conditions. The results of the phenotypic evaluation indicated that parental lines 93-11 (P1, a high tillering indica cultivar) and Milyang352 (P2, a low tillering japonica cultivar) showed distinctive phenotypic responses, also reflected in their derived population. In addition, the linkage mapping and quantitative trait locus (QTL) analysis detected three QTLs associated with tiller number on chromosome 2 (qTNN2-1, 130 cM, logarithm of the odds (LOD) 4.14, PVE 14.5%; and qTNL2-1, 134 cM, LOD: 6.05, PVE: 20.5%) and chromosome 4 (qTN4-1, 134 cM, LOD 3.92, PVE 14.5%), with qTNL2-1 having the highest phenotypic variation explained, and the only QTL associated with tiller number under low nitrogen cultivation conditions, using Kompetitive Allele-Specific PCR (KASP) and Fluidigm markers. The additive effect (1.81) of qTNL2-1 indicates that the allele from 93-11 (P1) contributed to the observed phenotypic variation for tiller number under low nitrogen cultivation. The breakthrough is that the majority of the candidate genes harbored by the QTLs qTNL2-1 and qTNN4-1 (here associated with the control of shoot branching under low and normal nitrogen cultivation, respectively), were also proposed to be involved in plant stress signaling or response mechanisms, with regard to their annotations and previous reports. Therefore, put together, these results would suggest that a possible crosstalk exists between the control of plant growth and development and the stress response in rice.

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Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape (Brassica napus L.)

Journal of Genetics ◽

10.1007/s12041-018-1044-3 ◽

2018 ◽

Vol 97 (5) ◽

pp. 1389-1406 ◽

Cited By ~ 3

Author(s):

Farshad Fattahi ◽

Barat Ali Fakheri ◽

Mahmood Solouki ◽

Christian Möllers ◽

Abbas Rezaizad

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Doubled Haploid ◽

Agronomic Traits ◽

Doubled Haploid Population ◽

Winter Oilseed Rape ◽

Brassica Napus L ◽

Mapping Qtl ◽

Haploid Population

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Mapping of grain alkali digestion trait using a Cheongcheong/Nagdong doubled haploid population in rice

Journal of Plant Biotechnology ◽

10.5010/jpb.2016.43.1.76 ◽

2016 ◽

Vol 43 (1) ◽

pp. 76-81 ◽

Cited By ~ 2

Author(s):

Hak Yoon Kim ◽

Kyung-Min Kim

Keyword(s):

Doubled Haploid ◽

Doubled Haploid Population ◽

Haploid Population ◽

Alkali Digestion

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Genetic Mapping of Quantitative Trait Loci for Yield-Affecting Traits in a Barley Doubled Haploid Population Derived from Clipper × Sahara 3771

Frontiers in Plant Science ◽

10.3389/fpls.2017.00688 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 6

Author(s):

Fatemeh Vafadar Shamasbi ◽

Seyed Hossein Jamali ◽

Behzad Sadeghzadeh ◽

Babak Abdollahi Mandoulakani

Keyword(s):

Quantitative Trait Loci ◽

Genetic Mapping ◽

Doubled Haploid ◽

Quantitative Trait ◽

Doubled Haploid Population ◽

Trait Loci ◽

Haploid Population

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Mapping Quantitative Trait Loci for High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 197734 Using a Doubled Haploid Population and Genotyping by Multiplexed Sequencing

Frontiers in Plant Science ◽

10.3389/fpls.2020.596962 ◽

2020 ◽

Vol 11 ◽

Author(s):

Lu Liu ◽

Congying Yuan ◽

Meinan Wang ◽

Deven R. See ◽

Xianming Chen

Keyword(s):

High Temperature ◽

Quantitative Trait Loci ◽

Spring Wheat ◽

Plant Resistance ◽

Doubled Haploid ◽

Quantitative Trait ◽

Adult Plant Resistance ◽

Adult Plant ◽

Doubled Haploid Population ◽

Haploid Population

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Characterization of QTL and eQTL controlling early Fusarium graminearum infection and deoxynivalenol levels in a Wuhan 1 x Nyubai doubled haploid wheat population

BMC Plant Biology ◽

10.1186/s12870-019-2149-4 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 2

Author(s):

François Fauteux ◽

Yunli Wang ◽

Hélène Rocheleau ◽

Ziying Liu ◽

Youlian Pan ◽

...

Keyword(s):

Fusarium Graminearum ◽

Doubled Haploid ◽

Expression Patterns ◽

Doubled Haploid Population ◽

Post Inoculation ◽

Head Blight ◽

Wheat Genotypes ◽

Wheat Population ◽

Fhb Resistance ◽

Moderately Resistant

Abstract Background Fusarium head blight (FHB) is a major disease of cereal crops, caused by the fungal pathogen Fusarium graminearum and related species. Breeding wheat for FHB resistance contributes to increase yields and grain quality and to reduce the use of fungicides. The identification of genes and markers for FHB resistance in different wheat genotypes has nevertheless proven challenging. Results In this study, early infection by F. graminearum was analyzed in a doubled haploid population derived from the cross of the moderately resistant wheat genotypes Wuhan 1 and Nyubai. Three quantitative trait loci (QTL) were identified: 1AL was associated with lower deoxynivalenol content, and 4BS and 5A were associated with reduced F. graminearum infection at 2 days post inoculation. Early resistance alleles were inherited from Wuhan 1 for QTL 1AL and 4BS and inherited from Nyubai for the 5A QTL. Cis and trans expression QTL (eQTL) were identified using RNA-seq data from infected head samples. Hotspots for trans eQTL were identified in the vicinity of the 1AL and 4BS QTL peaks. Among differentially expressed genes with cis eQTL within the QTL support intervals, nine genes had higher expression associated with FHB early resistance, and four genes had higher expression associated with FHB early susceptibility. Conclusions Our analysis of genotype and gene expression data of wheat infected by F. graminearum identified three QTL associated with FHB early resistance, and linked genes with eQTL and differential expression patterns to those QTL. These findings may have applications in breeding wheat for early resistance to FHB.

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Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding

Czech Journal of Genetics and Plant Breeding ◽

10.17221/3668-cjgpb ◽

2011 ◽

Vol 41 (No. 3) ◽

pp. 105-111 ◽

Cited By ~ 2

Author(s):

R. Koprna ◽

V. Kučera ◽

O. Kolovrat ◽

M. Vyvadilová ◽

M. Klíma

Keyword(s):

Erucic Acid ◽

Doubled Haploid ◽

Acid Content ◽

Seed Quality ◽

Quality Parameters ◽

Hplc Method ◽

Hybrid Breeding ◽

Erucic Acid Content ◽

Glucosinolate Content ◽

Brassica Napus L

Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different “00” quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 µmol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified.  

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