scholarly journals Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Elizabeth Hortêncio de Melo ◽  
Harrison Magdinier Gomes ◽  
Philip Noel Suffys ◽  
Márcia Quinhones Pires Lopes ◽  
Raquel Lima de Figueiredo Teixeira ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Essential Role ◽  
Allelic Diversity ◽  
Infectious Agent ◽  
The State ◽  
Dairy Herds ◽  
Genotypic Characterization ◽  
Epidemiological Tool

Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis represents a risk to human health. This study aimed to perform the genotypic characterization of M. bovis isolated from bovines diagnosed as tuberculosis from dairy herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for microbiological culture, and colonies compatible with Mycobacterium spp. were obtained in at least one culture from 17/30 granulomas. All isolates were confirmed to be M. bovis by spoligotyping and 24loci MIRU-VNTR typing. While spoligotyping characterized the isolates as SB0121, SB0295, SB0852, SB0120, and an unclassified genotype, 24loci MIRU-VNTR rendered two clusters of two isolates each and 13 unique profiles. Loci ETR-A showed higher discriminatory power, and loci (ETR-B, ETR-C, MIRU16, MIRU27, and QUB26) showed moderate allelic diversity. This is the first study on the genetic variability of the infectious agent cause of bovine TB in Pernambuco and demonstrates variability of strains in the state. Thus, it corroborates the importance of this microorganism as agent of bovine tuberculosis and its zoonotic potential, this epidemiological tool being a determinant in the rigor of the sanitary practices of disease control in dairy herds.

scholarly journals Genotypic characterization of Mycobacterium bovis isolates from dairy cattle

2020 ◽  
Author(s):  
Elizabeth Melo ◽  
Harrison Gomes ◽  
caro Santos ◽  
Jos Augusto Afonso ◽  
Mar lia Junqueira Franco ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Mycobacterium Bovis ◽  
Genotypic Characterization

scholarly journals Prevalence and herd-level risk factors of bovine tuberculosis in the State of Santa Catarina

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3659 ◽  
Author(s):  
Flávio Pereira Veloso ◽  
Karina Diniz Baumgarten ◽  
Ana Lourdes Arrais de Alencar Mota ◽  
Fernando Ferreira ◽  
José Soares Ferreira Neto ◽  
...  
Keyword(s):  
Risk Factors ◽  
Bovine Tuberculosis ◽  
Management Practices ◽  
Animal Health ◽  
Health Agency ◽  
The State ◽  
Dairy Herds ◽  
Santa Catarina ◽  
Significant Difference ◽  
The University

With the aim of supporting the strategic planning of the National Program for Control and Eradication of Brucellosis and Tuberculosis, different Brazilian states have been conducting cross-sectional studies, coordinated by the Ministry of Agriculture, Livestock, and Supply and with scientific support from the University of São Paulo and the University of Brasilia. In Santa Catarina, the State Animal Health Agency (CIDASC) conducted a study on bovine tuberculosis (TB) prevalence and assessment of risk factors in 2012. The state was divided into five regions and, in each region, independent sampling was performed in two steps: (i) cattle herds with reproductive activity were randomly selected; and (ii) in each herd, a sample of females aged 24 months or older underwent the intradermal comparative tuberculin test. A questionnaire was used to collect data on production characteristics and management practices that could be associated with the tuberculosis infection. Herd prevalence of bovine TB was 0.50% (95% confidence interval [CI]: 0.074–0.93%) while the prevalence of TB in adult females was 0.06% (95% CI: 0–0.12%). No significant difference in the prevalence of infected herds and of positive females was observed among the five regions. The logistic regression model revealed that herds with 19 or more females showed an odds ratio (OR) of 7.68 (95% CI: 1.22–48.39) compared to smaller herds, while dairy herds presented an OR of 10.43 (95% CI: 2.00–54.25) relative to beef or dual-purpose herds. The results suggest that dairy herds, in which animals are kept in partial or total confinement, and larger herds, which tend to acquire animals more often, are at a higher risk of bovine TB. Given the low prevalence and the type of higher-risk properties, a bovine TB surveillance system should be targeted at the state’s dairy basins, particularly the western region that accounts for the major industries and more intensive dairy farms.

scholarly journals Proposal of a Screening MIRU-VNTR Panel for the Preliminary Genotyping ofMycobacterium bovisin Mexico

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Enrique Bolado-Martínez ◽  
Iliana Benavides-Dávila ◽  
Maria del Carmen Candia-Plata ◽  
Moisés Navarro-Navarro ◽  
Magali Avilés-Acosta ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Causative Agent ◽  
Bovine Tuberculosis ◽  
Allelic Diversity ◽  
Geographical Regions ◽  
Wide Range ◽  
The World ◽  
Allelic Variability ◽  
High Discriminatory Power ◽  
Geographic Regions

Mycobacterium bovisis the major causative agent of bovine tuberculosis, one of the most relevant zoonoses in the world, and affects a wide range of wild and domesticated animals. Development of screening panels in mycobacterial genotyping, according to specific geographical regions, is strongly needed. The aim of this study is to select a panel, constituted by highly polymorphic MIRU-VNTRloci, to discriminate clinical isolates ofM. bovisin Mexico. In this study, 65 isolates ofM. bovisobtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24-locusMIRU-VNTR panel. The most polymorphiclociwere selected to build a panel with a high discriminatory power similar to the 24-locuspanel results. A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation. The selected MIRU-VNTR elements, according to the regional allelic variability, may be used in the preliminary genotyping ofMycobacterium bovisisolates in Mexico.

scholarly journals Epidemiologic characterization of bovine tuberculosis in the State of Rondônia, Brazil

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3639 ◽  
Author(s):  
Fabiano Benitez Vendrame ◽  
Marcos Amaku ◽  
Fernando Ferreira ◽  
Evelise Oliveira Telles ◽  
José Henrique Hildebrand Grisi-Filho ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Reproductive Activity ◽  
Cross Sectional Study ◽  
Fixed Number ◽  
The State ◽  
Cross Sectional ◽  
Apparent Prevalence ◽  
Primary Sample

A cross sectional study was performed between June 2009 and March 2010 to determine the situation of bovine tuberculosis (bTB) in Rondônia. The state was divided into three regions and, in each of them, 300 farms with reproductive activity were randomly chosen and considered as primary sample units. In the selected farms, an epidemiologic questionnaire was applied. A fixed number of bovine females older than two years of age was randomly selected and tested through comparative cervical tuberculin test. Considering the State of Rondônia, the apparent prevalence of bTB positive farms was 2.3% (95% CI = 1.5–3.5%). The prevalence in the regions varied from 1.7% (95% CI = 0.7 – 4%) to 3% (95% CI = 1.6–5.7%). The apparent prevalence of bTB positive animals in the State of Rondônia was 0.12% (95% CI = 0.06–0.25%) and varied from 0.08% (95% CI = 0.04–0.18%) to 0.15% (95% CI = 0.07–0.33%) in the regions. The risk factor associated to tuberculosis in the State of Rondônia was the acquisition of animals (OR = 7.1; 95% CI = 1.6–31.1). The State of Rondônia should implement a surveillance system to detect bTB-infected herds to certify them as bTB-free. Moreover, an efficient health education program to inform farmers to test replacement animals for bTB prior to introduction in their herds should also be implemented.

scholarly journals First approach to molecular epidemiology of bovine tuberculosis in Colombia

2016 ◽  
pp. 5222-5236 ◽  
Author(s):  
Jimena Jojoa-Jojoa ◽  
Maira Wintaco M ◽  
Francisco Osorio R ◽  
Gloria Puerto-Castro ◽  
Martha Guerrero-Guerrero
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Microbiological Analysis ◽  
Mycobacterium Species ◽  
Molecular Assays ◽  
Nasal Swabs ◽  
The World ◽  
Gross Lesions

ABSTRACT Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.RESUMEN Objetivo. Investigar la presencia de Mycobacterium bovis y otras especies de Mycobacterium en muestras de ganado bovino y búfalino en Colombia e iniciar la caracterización molecular de M. bovis en el país. Materiales y métodos. 492 muestras fueron recogidas de hatos identificados con presencia de animales infectados a través de la prueba de PPD, por el Grupo de Tuberculosis Bovina del Instituto Colombiano Agropecuario ICA en ocho departamentos de Colombia. Se incluyeron ganglios linfáticos de cabeza, tórax y abdomen, tejidos con lesiones macroscópicas de tuberculosis, hisopos nasales, leche, sangre y quesos frescos. Las muestras se sometieron a investigación de Mycobacterium bovis y otras micobacterias mediante el análisis microbiológico convencional y pruebas moleculares de PCR-IS6110 y spoligotyping. Resultados. En las muestras analizadas especialmente en los ganglios linfáticos, se evidenció Mycobacterium bovis con genotipos reportados y no reportados previamente en el mundo, así como M. tuberculosis en los departamentos de Antioquia, Cundinamarca, Magdalena y Boyacá. Conclusiones. En Colombia existen al menos 7 genotipos de M. bovis que se encuentran infectando y enfermando a bovinos y bufalinos de cuatro diferentes departamentos del país constituyéndose en seria amenaza para la salud pública.

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