scholarly journals Effects of Nerve Growth Factor-β From Bull Seminal Plasma on Steroidogenesis and Angiogenic Markers of the Bovine Pre-ovulatory Follicle Wall Cell Culture

2022 ◽  
Vol 8 ◽  
Author(s):  
Jamie L. Stewart ◽  
Liying Gao ◽  
Jodi A. Flaws ◽  
Vitor R. G. Mercadante ◽  
Nicholas W. Dias ◽  
...  

Nerve growth factor-β (NGF) is critical for ovulation in the mammalian ovary and is luteotrophic when administered systemically to camelids and cattle. This study aimed to assess the direct effects of purified bovine NGF on steroidogenesis and angiogenic markers in the bovine pre-ovulatory follicle. Holstein heifers (n = 2) were synchronized with a standard protocol, and heifers with a preovulatory follicle (≥ 12 mm) had the ovary containing the dominant follicle removed via colpotomy. Pre-ovulatory follicles were dissected into 24 pieces containing theca and granulosa cells that were randomly allocated into culture media supplemented with either purified bovine NGF (100 ng/mL) or untreated (control) for 72 h. The supernatant media was harvested for quantification of progesterone, testosterone, and estradiol concentrations, whereas explants were subjected to mRNA analyses to assess expression of steroidogenic and angiogenic markers. Treatment of follicle wall pieces with NGF upregulated gene expression of steroidogenic enzyme HDS17B (P = 0.04) and increased testosterone production (P < 0.01). However, NGF treatment did not alter production of progesterone (P = 0.81) or estradiol (P = 0.14). Consistently, gene expression of steroidogenic enzymes responsible for producing these hormones (STAR, CYP11A1, HSD3B, CYP17A1, CYP19A1) were unaffected by NGF treatment (P ≥ 0.31). Treatment with NGF downregulated gene expression of the angiogenic enzyme FGF2 (P = 0.02) but did not alter PGES (P = 0.63), VEGFA (P = 0.44), and ESR1 (P = 0.77). Collectively, these results demonstrate that NGF from seminal plasma may interact directly on the theca and granulosa cells of the bovine pre-ovulatory follicle to stimulate testosterone production, which may be secondary to theca cell proliferation. Additionally, decreased FGF2 expression in NGF-treated follicle wall cells suggests hastened onset of follicle wall cellular remodeling that occurs during early luteal development.

2020 ◽  
Author(s):  
Jamie L. Stewart ◽  
Liying Gao ◽  
Jodi A. Flaws ◽  
Vitor R.G. Mercadante ◽  
Nicholas W. Dias ◽  
...  

Abstract Nerve growth factor-β (NGF) is critical for ovulation in the mammalian ovary and is luteotrophic when administered systemically to camelids and cattle. This study aimed to assess the direct effects of purified bovine NGF on steroidogenesis and angiogenic markers in the bovine pre-ovulatory follicle. A chort of Holstein heifers were synchronized with a standard protocol and heifers with the preovulatory follicle (≥ 12 mm) had an the ovary containing the dominant follicle removed via colpotomy. Pre-ovulatory follicles were dissected in 24 pieces containing theca and granulosa cells that were randomly allocated to receive either cultured in media supplemented with purified bovine NGF (100 ng/mL) or untreated (control) for 72 h. The supernatant media was harvested for determination of progesterone, testosterone, and estradiol, whereas explants were used for mRNA analyses for steroidogenesis and angiogenic markers. Treatment of follicle tissue with NGF upregulated gene expression of steroidogenic enzyme HDS17B ( P = 0.04) and increased testosterone production ( P < 0.01). However, NGF treatment did not alter production of progesterone ( P = 0.81) or estradiol ( P = 0.14). Consistently, gene expression of steroidogenic enzymes responsible for producing these hormones ( STAR , CYP11A1 , HSD3B , CYP17A1 , CYP19A1 ) were unaffected by NGF treatment ( P ≥ 0.31). Treatment with NGF downregulated gene expression of the angiogenic enzyme FGF2 ( P = 0.02) but did not alter PGES ( P = 0.63), VEGFA ( P = 0.44), and ESR1 ( P = 0.77). Collectively, these results demonstrate that NGF from seminal plasma may interact directly with the bovine pre-ovulatory follicle to alter downstream steroidogenesis and luteal development.


Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1558
Author(s):  
Margherita Maranesi ◽  
Francesco Alessandro Palermo ◽  
Antonello Bufalari ◽  
Francesca Mercati ◽  
Daniele Paoloni ◽  
...  

The grey squirrel is an invasive alien species that seriously threatens the conservation of the native red squirrel species. With the aim of characterizing the reproductive physiology of this species due to its great reproductive success, the function of the ovarian nerve growth factor (NGF) system was analyzed in a grey squirrel population living in central Italy. During the breeding and nonbreeding seasons, the ovarian presence, distribution, and gene expression of NGF, neurotrophic tyrosine kinase receptor 1 (NTRK1), and nerve growth factor receptor (NGFR), as well as NGF plasma concentrations, were evaluated in female grey squirrels. NGF was found in the luteal cells and in the thecal and granulosa cells of follicles, while NTRK1 and NGFR were only observed in follicular thecal and granulosa cells. NGF and NGFR transcripts were almost two-fold greater during the breeding season, while no seasonal differences were observed in NTRK1 gene expression. During the breeding season, NGFR was more expressed than NTRK1. Moreover, no changes were observed in NGF plasma levels during the reproductive cycle. The NGF system seems to be involved in regulating the ovarian cycle mainly via local modulation of NGF/NGFR, thus playing a role in the reproductive physiology of this grey squirrel population.


2018 ◽  
Vol 197 ◽  
pp. 335-342 ◽  
Author(s):  
Jamie L. Stewart ◽  
Igor F. Canisso ◽  
Robyn E. Ellerbrock ◽  
Vitor R.G. Mercadante ◽  
Fabio S. Lima

2007 ◽  
Vol 5 (8) ◽  
pp. 1715-1725 ◽  
Author(s):  
A. GUTIÉRREZ-FERNÁNDEZ ◽  
R. J. PARMER ◽  
L. A. MILES

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