scholarly journals Accumulation of Phenolic Compounds and Glucosinolates in Sprouts of Pale Green and Purple Kohlrabi (Brassica oleracea var. gongylodes) under Light and Dark Conditions

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1939
Author(s):  
Ramaraj Sathasivam ◽  
Min Cheol Kim ◽  
Hyeon Ji Yeo ◽  
Bao Van Nguyen ◽  
Soo In Sohn ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Brassica Oleracea ◽  
Root Length ◽  
Dry Weight ◽  
Light Treatment ◽  
Total Phenolic ◽  
Light Conditions ◽  
Fresh Weight ◽  
Dark Conditions ◽  
Pale Green

Kohlrabi is considered an important dietary vegetable worldwide. In this study, we investigated the growth and accumulation of phenolic compounds (PCs) and glucosinolates in sprouts of pale green and purple kohlrabi (Brassica oleracea var. gongylodes) in response to light and dark conditions. Pale green kohlrabi presented high fresh weight and root length irrespective of light treatment, whereas under dark conditions, it presented higher fresh weight and shoot length than purple kohlrabi. In contrast, the root length of both kohlrabies increased markedly under light conditions compared to that under dark conditions. Thirteen PCs and eight glucosinolates were detected and quantified in 10-day-old pale green and purple kohlrabies. In both kohlrabies, the individual and total phenolic levels were much higher under the light treatment than under the dark treatment. Under light and dark conditions, the total phenolic content was 6362.13 and 5475.04 µg/g dry weight in the pale green kohlrabi, respectively, whereas in the purple kohlrabi, it was 10,115.76 and 9361.74 µg/g dry weight, respectively. Dark conditions favored higher accumulation of glucosinolates than light conditions. Progoitrin, neoglucobrassicin, glucoerucin, and 4-methoxyglucobrassicin were the predominant glucosinolates in both kohlrabies and were present in much higher amounts in the pale green kohlrabi. In pale green kohlrabi under dark conditions, the total glucosinolates content was 4.75 and 2.62 times higher than that of the purple kohlrabi under light and dark conditions, respectively. Among individual glucosinolates, in the pale green kohlrabi under the dark condition, progoitrin was found to have the highest content, which was 90.28 and 54.51 times higher than that in the purple kohlrabi under light and dark conditions, respectively. These results show that the phenolic and glucosinolates levels varied widely, and these variations between the two types of kohlrabi under both light and dark conditions were significant. Our findings suggest that light and dark conditions enhance the accumulation of PCs and glucosinolates, respectively, during the development of kohlrabi seedlings.

scholarly journals Screening for Innovative Sources of Carotenoids and Phenolic Antioxidants among Flowers

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2625
Author(s):  
Antonio J. Meléndez-Martínez ◽  
Ana Benítez ◽  
Mireia Corell ◽  
Dolores Hernanz ◽  
Paula Mapelli-Brahm ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Vitamin A ◽  
Phenolic Content ◽  
Dry Weight ◽  
Punica Granatum ◽  
Total Phenolic ◽  
Phenolic Antioxidants ◽  
Fresh Weight ◽  
Phenolic Contents

Flowers have been used for centuries in decoration and traditional medicine, and as components of dishes. In this study, carotenoids and phenolics from 125 flowers were determined by liquid chromatography (RRLC and UHPLC). After comparing four different extractants, the carotenoids were extracted with acetone: methanol (2:1), which led to a recovery of 83%. The phenolic compounds were extracted with 0.1% acidified methanol. The petals of the edible flowers Renealmia alpinia and Lantana camara showed the highest values of theoretical vitamin A activity expressed as retinol activity equivalents (RAE), i.e., 19.1 and 4.1 RAE/g fresh weight, respectively. The sample with the highest total phenolic contents was Punica granatum orange (146.7 mg/g dry weight). It was concluded that in most cases, flowers with high carotenoid contents did not contain high phenolic content and vice versa. The results of this study can help to develop innovative concepts and products for the industry.

scholarly journals Effect of cytokinins on growth and phenylpropanoid accumulation in Tartary buckwheat sprouts (Fagopyrum esculentum Moench)

2021 ◽  
pp. 89-94
Author(s):  
Ramaraj Sathasivam ◽  
Min Cheol Kim ◽  
Yong Suk Chung ◽  
Sang Un Park
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Root Length ◽  
Growth Parameters ◽  
Hormonal Treatment ◽  
Tartary Buckwheat ◽  
Total Phenolic ◽  
Fresh Weight ◽  
Fagopyrum Esculentum Moench ◽  
Hormonal Concentration

This study analyzed the effect of plant hormones, zeatin, 6-benzyl amino purine (BAP), kinetin, and thidiazuron (TDZ) on the growth of Tartary buckwheat sprouts and analyzed the fresh weight, shoot and root length, and production of phenolic compounds. All the hormone-treated plants at the lowest concentration (0.1 mg/L) showed the highest levels of growth parameters (fresh weight, shoot, and root length) when compared to the control. Among the various hormones treatment, the plant treated with 1 mg/L of BAP, kinetin, and zeatin showed the highest total phenolic level, whereas the TDZ showed the highest accumulation of total phenolic at the lowest concentration (0.1 mg/L). A total of 6 compounds were identified (4-hydroxybenzoic acid, caffeic acid, chlorogenic acid, p-coumaric acid, rutin, and trans-cinnamic acid) were quantified by high liquid performance chromatography (HPLC) after treatment of plant with different concentrations of hormones. Among these individual phenolic compounds, at the higher hormonal concentration (1 mg/L) the rutin showed the highest accumulation in BAP, zeatin, and kinetin treated sprout, whereas in the TDZ treated sprout the rutin content was highest at the lowest concentration (0.1 mg/L). From these results, it is suggested that BAP, zeatin, and kinetin at the lowest concentrations might positively enhance the growth of buckwheat sprouts, whereas at the highest hormonal treatment the accumulation of the phenolic compounds was higher. However, in TDZ treatment the growth and phenolic compound accumulation were highest at the lowest concentration. From these results, it is showed that suitable concentrations might enhance the growth and phenolic compound accumulation in Tatary buckwheat sprout.

scholarly journals Growth and yield of kale (Brassica oleracea var. Acephala) on different vermicompost dosages and plant spacings

2019 ◽  
Vol 6 (3) ◽  
pp. 394-402
Author(s):  
Saraswati Kurnia Dewanti ◽  
Eny Fuskhah ◽  
Sutarno
Keyword(s):  
Factorial Design ◽  
Brassica Oleracea ◽  
Plant Height ◽  
Root Length ◽  
Crop Production ◽  
Dry Weight ◽  
Growth And Yield ◽  
Plant Spacing ◽  
Fresh Weight ◽  
Agricultural Sciences

This study aimed to examine the effect and interaction between vermicompost dosage and plantspacing on kale growth and yield. The research was held on March – June 2019 in Tegalsari,Sidomukti, Semarang Regency and at Ecology and Crop Production Laboratory, Faculty of Animaland Agricultural Sciences, Diponegoro University, Semarang. The study used a completelyrandomized factorial design 4 x 2 with 3 replications. The first factor was the vermicompost dosage of100 kg N/Ha (A1), 150 kg N/Ha (A2), 200 kg N/Ha (A3), and 250 kg N/Ha (A4). The second factorwas plant spacing of 20 x 40 cm (B1) and 30 x 40 cm (B2). Data were analyzed by variance andproceeded with DMRT test at level 5%. The observed variables were plant height, amount of leaves,chlorophyll, root length, fresh weight, and dry weight. The results showed that different dosages ofvermicompost and plant spacing only affected chlorophyll and root length of kale. The highestchlorophyll was attained at the treatment of 150 kg N/Ha vermicompost dosage and 20 x 30 cm plantspacing, equal to a treatment of 250 kg N/Ha vermicompost dosage and 30 x 40 cm plant spacing.The highest root length was attained at the treatment of 250 kg N/Ha vermicompost dosage and 30 x40 cm plant spacing, equal to the treatments of 100 kg N/Ha vermicompost dosage and 30 x 40 cmplant spacing and 200 kg N/Ha vermicompost dosage and 20 x 40 cm plant spacing.

scholarly journals Effects of Light-Emitting Diodes on the Accumulation of Glucosinolates and Phenolic Compounds in Sprouting Canola (Brassica napus L.)

Foods ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 76 ◽  
Author(s):  
Chang Park ◽  
Nam Kim ◽  
Jong Park ◽  
Sook Lee ◽  
Jong-Won Lee ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Phenolic Compounds ◽  
Light Emitting Diode ◽  
Red Light ◽  
Dry Weight ◽  
Total Phenolic ◽  
Brassica Napus L ◽  
Light Emitting ◽  
Led Light ◽  
Sprout Growth

In this study, we investigated optimal light conditions for enhancement of the growth and accumulation of glucosinolates and phenolics in the sprouts of canola (Brassica napus L.). We found that the shoot lengths and fresh weights of red light-irradiated sprouts were higher than those of sprouts exposed to white, blue, and blue + red light, whereas root length was not notably different among red, blue, white, and blue + red light treatments. The accumulations of total glucosinolates in plants irradiated with white, blue, and red lights were not significantly different (19.32 ± 0.13, 20.69 ± 0.05, and 20.65 ± 1.70 mg/g dry weight (wt.), respectively). However, sprouts exposed to blue + red light contained the lowest levels of total glucosinolates (17.08 ± 0.28 mg/g dry wt.). The accumulation of total phenolic compounds was the highest in plants irradiated with blue light (3.81 ± 0.08 mg/g dry wt.), 1.33 times higher than the lowest level in plants irradiated with red light (2.87 ± 0.05 mg/g dry wt.). These results demonstrate that red light-emitting diode (LED) light is suitable for sprout growth and that blue LED light is effective in increasing the accumulation of glucosinolates and phenolics in B. napus sprouts.

The Effect of Heating Temperature on Total Phenolic Content, Antioxidant Activity, and Phenolic Compounds of Plum and Mahaleb Fruits

2019 ◽  
Vol 15 (11-12) ◽  
Author(s):  
Kashif Ghafoor ◽  
Isam A. Mohamed Ahmed ◽  
Süleyman Doğu ◽  
Nurhan Uslu ◽  
Gbemisola J. Fadimu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Heating Temperature ◽  
Dry Weight ◽  
Total Phenolic ◽  
Dihydroxybenzoic Acid ◽  
Different Temperatures

AbstractThe effect of heating at different temperatures (60, 80, 90, 110, and 130 °C) on the total phenolic content, antioxidant activity, and phenolic compounds present in plum and mahaleb fruits was investigated. The antioxidant activity values and total phenolic contents of fresh plum (93.82% measured by DPPH method, 787.79 mg gallic acid equivalent (GAE)/100 g dry weight determined by Folin method) and mahaleb fruits (81.80%, 634.47 mg GAE/100 g dry weight) were higher than plum and mahaleb fruits dried at different temperatures (p < 0.05). Generally, the heating process caused a reduction in both total phenolic content and antioxidant activity for plum and mahaleb. While (+)-catechin (92.62 mg/kg), 1,2-dihydroxybenzene (132.15 mg/kg), gallic acid (107.01 mg/kg), and 3,4-dihydroxybenzoic acid (74.59 mg/kg) are the key phenolic compounds in fresh plum, (+)-catechin, 3,4-dihydroxybenzoic acid, 1,2-dihydroxybenzene, and syringic and caffeic acids were the major phenolic compounds of mahaleb fruits. The polyphenol content of fruits and the class of phenolics present are significantly affected by heating temperature.

scholarly journals Variety and Year Impact on Phenolic Content of Arkansas-Grown Sweetpotato Leaves

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 371-371
Author(s):  
Josue Bolanos ◽  
Sun-Ok Lee ◽  
Luke Howard ◽  
Cindi Brownmiller ◽  
Shahidul Islam ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Statistical Significance ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antioxidant Potential ◽  
Total Phenolic

Abstract Objectives Sweetpotato leaf (SPL) is a natural source of phenolic compounds with potential utility as an antioxidant. The study aimed to measure the impacts of the years on SPL total phenolic content and antioxidant capacity and to identify and quantify the individual phenolic compounds. Methods Sweetpotato leaves in 2018 (27 varieties) and 2019 (24 varieties) were grinded and lyophilized. Phenolic compounds were extracted with 70% ethanol from SPLs. Total phenolic content was determined by Folin-Ciocalteu method and antioxidant potential was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Five SPL samples with statistically highest antioxidant capacity were identified and quantified by HPLC. All statistical analyses were carried out by SAS software using ANOVA. Statistical significance was accepted at P &lt; 0.05. Results The average of total phenolic contents in 2018 SPL samples was 43 ± 13 mg gallic acid equivalent (GAE)/g dry weight (DW) SPL whereas in 2019, it was 53 ± 9 mg GAE/g dry weight SPL. Antioxidant capacity in 2018 was 110 ± 75 µmol Trolox equivalent (TE)/g dry weight SPL whereas in 2019, it was 132 ± 32 µmol TE/g dry weight SPL. SPL1 had the highest antioxidant potential, followed by SPL9, SPL3, SPL28, and SPL11 (P &lt; 0.05). The concentrations of major identified phenolics from the five SPL samples (#1, 9, 3, 28, and 11) in dry weight of SPL were: chlorogenic acid at 3.05 ± 0.35 mg/g, 5-monocaffeoylquinic acid (CQA) at 0.71 ± 0.06 mg chlorogenic acid equivalent (ChAE)/g, 4-CQA at 0.99 ± 0.07 mg ChAE/g, 3,4-diCQA at 1.22 ± 0.18 mg ChAE/g, 3,4,5-triCQA at 1.2 ± 0.18 mg ChAE/g, and 3,5-diCQA at 15.5 ± 4.05 mg ChAE/g. 3,5-diCQA, the predominant phenolic, was present in the highest amounts in SPL1. Conclusions The results showed that sweetpotato leaves collected in 2019 contained higher total phenolics and antioxidant capacity than the ones collected in 2018. Phenolic compounds have strong antioxidant activity in Arkansas-grown sweetpotato leaves. This study warrants further investigation of sweetpotato leaves to be utilized as an antioxidant. Funding Sources The work was supported by USDA-NIFA.

scholarly journals Variation of Glucosinolate Accumulation and Gene Expression of Transcription Factors at Different Stages of Chinese Cabbage Seedlings under Light and Dark Conditions

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Yeon Bok Kim ◽  
Jin-Hyuk Chun ◽  
Hye Ran Kim ◽  
Sun-Ju Kim ◽  
Yong Pyo Lim ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Chinese Cabbage ◽  
High Performance ◽  
Dry Weight ◽  
Seedling Development ◽  
Leafy Vegetables ◽  
Light Conditions ◽  
Quantitative Real Time Pcr ◽  
Dark Conditions ◽  
Almost All

Chinese cabbage is one of the most important leafy vegetables widely used in East Asian cuisines. The glucosinolate (GSL) accumulation and transcript levels of 7 transcription factors (Dofl.l, IQD1-1, MYB28, MYB29, MYB34, MYB51, and MYB122, and their isoforms) involved in the biosynthesis of aliphatic and indolic glucosinolates (GSLs) were analyzed at different stages of Chinese cabbage (Brassica rapa ssp. pekinensis) seedlings under light and dark conditions using high performance liquid chromatography and quantitative real time PCR. During seedling development, transcription of almost all transcription factors under light conditions was higher expressed than under dark conditions. Five aliphatic GSLs (progoitrin, sinigrin, glucoalyssin, gluconapin, and glucobrassicanapin) and four indolic GSLs (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrasscin) were detected. Total GSL contents under light conditions 6, 8, and 10 days after sowing (DAS) were 3.2-, 3.9-, and 6.9-fold higher, respectively than those of dark conditions. Interestingly, total GSL contents 2 {85.4 μmol/g dry weight (DW)} to 10 (7.74 μmol/g DW) DAS under dark conditions were gradually decreased. In this study, our results suggest that light affects the levels of GSL in Chinese cabbage seedlings. These results could be useful for obtaining cabbage varieties rich in GSLs.

Utilization of kudzu as a lead phytoremediator and the impact of lead on selected physiological responses

2013 ◽  
Vol 93 (5) ◽  
pp. 951-959 ◽  
Author(s):  
Kristin Schwarzauer-Rockett ◽  
Safaa H. Al-Hamdani ◽  
James R. Rayburn ◽  
Nixon O. Mwebi
Keyword(s):  
Phenolic Compounds ◽  
Stomatal Conductance ◽  
Lead Concentration ◽  
Ethylenediaminetetraacetic Acid ◽  
Physiological Responses ◽  
Dry Weight ◽  
Control Treatment ◽  
Total Phenolic ◽  
Plant Responses ◽  
The Impact

Schwarzauer-Rockett, K., Al-Hamdani, S. H., Rayburn, J. R. and Mwebi, N. O. 2013. Utilization of kudzu as a lead phytoremediator and the impact of lead on selected physiological responses. Can. J. Plant Sci. 93: 951–959. This study was carried out to evaluate kudzu [Pueraria montana var. lobata (Willd.)] in lead phytoremediation. The impact of lead (PbNO3) concentrations of 100 and 200 mg L−1 on plant growth, photosynthetic pigments, photosynthesis, and stomatal conductance were evaluated. Additionally, concentrations of phenolic compounds and anthocyanin were determined. The lead was dissolved in 0.142 g L−1 ethylenediaminetetraacetic acid (EDTA) and an EDTA control treatment was added to evaluate the impact of EDTA on the above selected plant responses. Root accumulation of lead was significantly higher than in the shoot. Kudzu growth in response to the presence of lead and EDTA in the Hoagland's solution was similar to that of the EDTA control, except for plants growing at 200 mg L−1, which showed significantly lower root dry weight. Total phenolic compounds increased with the presence of EDTA and lead in the Hoagland's solution. Photosynthetic rate, stomatal conductance, chlorophyll a and chlorophyll b, carotenoids, and anthocyanin were not different (P≤0.05) among the treatments, with the exception that carotenoids were significantly higher in plants growing in the presence of EDTA without lead compared with 200 mg L−1 lead concentration. Kudzu accumulated most of the lead in the root and therefore can be considered as a rhizofiltrator. As kudzu was able to accumulate 1.02% (wt/wt) of lead it can be considered a hyper-accumulator.

scholarly journals Inhibitory Effect ofLoranthus parasiticuson IgE-Mediated Allergic Responses in RBL-2H3 Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Jae-Myung Yoo ◽  
Ju-Hye Yang ◽  
Young Soo Kim ◽  
Won-Kyung Cho ◽  
Jin Yeul Ma
Keyword(s):  
Phenolic Compounds ◽  
Late Phase ◽  
Water Extract ◽  
Dry Weight ◽  
Interleukin 4 ◽  
Total Phenolic ◽  
Prostaglandin E ◽  
Dependent Manner ◽  
Antiallergic Activity ◽  
Ige Mediated

The mistletoeLoranthus parasiticushas been used as a compound for traditional medicine in Northeast Asia for a long time and is known to possess neuroprotective action. Nonetheless, the effect ofLoranthus parasiticuson allergic responses remains unknown. In the present study, we evaluated whether the water extract ofLoranthus parasiticus(LPE) could inhibit IgE-mediated allergic responses in RBL-2H3 cells. LPE inhibited the release ofβ-hexosaminidase (IC50, 184.5 μg/mL) and the formation of tumor necrosis factor-α(IC50, 84.27 μg/mL), interleukin-4 (IC50, 93.43 μg/mL), prostaglandin E2(IC50, 84.10 μg/mL), prostaglandin D2, and leukotriene C4(IC50, 43.27 μg/mL) in a concentration-dependent manner. Moreover, LPE inhibited phosphorylation of Syk, PLCγ1/2, PKCδ, ERK, JNK, p38, and Akt. In the late phase, LPE decreased 5-lipoxygenase phosphorylation and COX-2 expression but not cPLA2phosphorylation. Additionally, LPE included total phenolic compounds (10.72 mg/g dry weight) and total flavonoids (56.20 mg/g dry weight). These results suggest that the phenolic compounds or flavonoids contained in LPE may be associated with antiallergic activity. The phenolic compounds and flavonoids in LPE are antiallergic phytochemicals capable of inhibiting the activation of the FcεRI signaling cascade in mast cells. Such effects may provide further information for the development of a phytomedicine for allergic diseases.

scholarly journals The Hormonal Seed Priming in Relation to Carrot Germination

2020 ◽  
Vol 1 (6) ◽  
pp. 1-5
Author(s):  
Filza Ghafoor ◽  
Sana Liaqat ◽  
Waseem Iqbal
Keyword(s):  
Root Length ◽  
Dry Matter ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Matter Content ◽  
Dry Matter Content ◽  
Fresh Weight ◽  
Stress Effects ◽  
Seedling Length

Seed priming is a pre-sowing treatment used to reduce stress effects in crops .This technique has been used to increase the germination rate of seeds mainly under unfavorable environmental condition. Crop productivity faces many problems like that unavailability of suitable condition that causes unbalanced seedling growth and poor germination. Hormones are essential to improve plant growth, development, crop yield quality and quantity. Auxin is essential hormones that improves germination of seeds and reduce stress effects. Laboratory experiment was conducted to study the effect of seed priming using different concentrations of IAA and IBA on carrot seeds (Daucus carota). In laboratory experiment, three concentrations of IAA and IBA (75 ppm, 50ppm, 25ppm) treated with distilled water with priming duration of 24 hours. Experiment was laid out in CRD with three replications. Data was collected to investigate the effect of seed priming with IAA and IBA under these condition like that (fresh weight, dry weight, speed of germination, root length, seed length, seedling length, final germination rate, mean germination time and dry matter content). Maximum fresh weight, final germination percentage, seedling length, energy of germination, speed of germination and mean germination rate was recorded IBA 75ppm. Maximum dry weight and dry matter content was observed IBA 25ppm.Maximum shoot length was recorded IBA 50ppm. Non-significant difference was recorded in root length.

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