scholarly journals Metabolomic Analysis and Identification of Sperm Freezability-Related Metabolites in Boar Seminal Plasma

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1939
Author(s):  
Yuting Zhang ◽  
Hanlin Liang ◽  
Yan Liu ◽  
Meng Zhao ◽  
Qianqian Xu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Aspartic Acid ◽  
Seminal Plasma ◽  
Metabolomic Analysis ◽  
Sperm Cryopreservation ◽  
Accessory Sex Glands ◽  
Plasma Metabolome ◽  
Significant Difference ◽  
Boar Sperm ◽  
Boar Semen

Some potential markers of boar sperm freezability have been found in spermatozoa, but little attention has been paid to seminal plasma. The seminal plasma is composed of secretions from the testis, epididymis, and accessory sex glands. The exposure of spermatozoa to small molecules such as metabolites can affect sperm function. However, details and significance of the seminal plasma metabolome related to boar sperm freezability are unknown. Therefore, the main aim of this study was to explore the differences in the metabolic level of seminal plasma between boars with differential freezability and to explore the candidate biomarkers of semen freezability. A total of 953 metabolites were identified in boar semen plasma by UHPLC-qTOF-MS analysis, and 50 metabolites showed significant change between the GFE group and PFE group. Further, twelve metabolites were subjected to metabolic target analysis, and three metabolites (D-aspartic acid, N-acetyl-L-glutamate (NAG), and inosine) showed differences. In conclusion, there is significant difference in the metabolome of seminal plasma between GFE and PFE individuals. D-aspartic acid, NAG, and inosine in seminal plasma may be potential markers for assessing sperm cryopreservation resistance in boars.

Metabolomic Analysis and Identification of Sperm Freezability Biomarkers in Boar Seminal Plasma

2021 ◽  
Author(s):  
Yu-ting Zhang ◽  
Han-lin Liang ◽  
Yan Liu ◽  
Meng Zhao ◽  
Qian-qian Xu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Aspartic Acid ◽  
Seminal Plasma ◽  
Freezing Process ◽  
Accessory Sex Glands ◽  
Plasma Metabolome ◽  
Boar Sperm ◽  
Boar Semen ◽  
The Difference ◽  
Sperm Functions

Abstract Background: In the freezing process of boar sperm, there are obvious differences of freezability between individuals. Studies suggest that specific freezability markers might be useful in good (GFE) and poor freezabitity ejaculates (PFE) selection prior to cryopreservation. Some potential markers of boar sperm freezability have been found from spermatozoa, little attention has been paid to seminal plasma. The seminal plasma is composed of secretions from testis, epididymis, and accessory sex glands, and the exposure of spermatozoa to small molecules such as metabolites can affect the sperm functions. However, details and significance of the seminal plasma metabolome related to boar sperm freezability are unknown. Therefore, the main aim of this study was to explore the difference in the metabolic level of seminal plasma between boars with differential freezability, and to explore the biomarkers of semen freezing tolerance. Results: A total of 953 metabolites were identified in boar semen plasma by UHPLC-qTOF-MS analysis, and 50 metabolites show significant change between GFE group and PFE group. Further, twelve metabolites were subjected to metabolic target analysis and three metabolites (D-Aspartic acid, N-Acetyl-L-glutamate (NAG), and Inosine) show differences.Conclusions: There is significant differece on metabolome of seminal plasma between GFE and PFE individuals. The D-Aspartic acid, NAG, and Inosine in seminal plasma may be potential markers for assessing sperm cryopreservation resistance in boars.

scholarly journals Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 203
Author(s):  
María Gemma Millán de la Blanca ◽  
Eva Martínez-Nevado ◽  
Cristina Castaño ◽  
Juncal García ◽  
Berenice Bernal ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Genetic Material ◽  
First Year ◽  
Sperm Cryopreservation ◽  
Straight Line ◽  
The Family ◽  
Second Year ◽  
American Flamingo ◽  
Phoenicopterus Ruber

The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen–thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µm/s vs. 16.64 µm/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01); but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos; seminal plasma removal did not provide a benefit for sperm cryopreservation.

scholarly journals Thawing boar semen in the presence of seminal plasma improves motility, modifies subpopulation patterns and reduces chromatin alterations

2017 ◽  
Vol 29 (8) ◽  
pp. 1576 ◽  
Author(s):  
Rocío Fernández-Gago ◽  
Manuel Álvarez-Rodríguez ◽  
Marta E. Alonso ◽  
J. Ramiro González ◽  
Beatriz Alegre ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Chromatin Structure ◽  
Sperm Chromatin ◽  
Chromatin Compaction ◽  
Sperm Analysis ◽  
Positive Effects ◽  
Fragmentation Index ◽  
Non Linear ◽  
Boar Semen ◽  
Dna Fragmentation Index

Seminal plasma could have positive effects on boar semen after thawing. In the present study we investigated changes in the motility and chromatin structure in spermatozoa over 4 h incubation (37°C) of boar semen thawed in the presence of 0%, 10% or 50% seminal plasma from good-fertility boars. Cryopreserved doses were used from seven males, three of which were identified as susceptible to post-thawing chromatin alterations. Motility was analysed by computer-aided sperm analysis every hour, and data were used in a two-step clustering, yielding three subpopulations of spermatozoa (slow non-linear, fast non-linear, fast linear). Chromatin structure was analysed using a sperm chromatin structure assay and flow cytometry to determine the DNA fragmentation index (%DFI) as a percentage, the standard deviation of the DFI (SD-DFI) and the percentage of high DNA stainability (%HDS), indicating chromatin compaction. Thawing without seminal plasma resulted in a rapid loss of motility, whereas seminal plasma helped maintain motility throughout the incubation period and preserved the subpopulation comprising fast and linear spermatozoa. The incidence of chromatin alterations was very low in samples from non-susceptible males, whereas samples from males susceptible to post-thawing chromatin alterations exhibited marked alterations in %DFI and %HDS. Seminal plasma partly prevented these alterations in samples from susceptible males. Overall, 50% seminal plasma was the most efficient concentration to protect motility and chromatin. Some changes were concomitant with physiological events reported previously (e.g., semen thawed with 50% seminal plasma increased the production of reactive oxygen species and yielded higher fertility after AI). Thawing in the presence of seminal plasma could be particularly useful in the case of samples susceptible to post-thawing chromatin damage.

scholarly journals A proton NMR study of the effect of a new intravasal injectable male contraceptive RISUG on seminal plasma metabolites

Reproduction ◽  
2001 ◽  
pp. 431-436 ◽  
Author(s):  
U Sharma ◽  
K Chaudhury ◽  
NR Jagannathan ◽  
SK Guha
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Seminal Plasma ◽  
Vas Deferens ◽  
Plasma Metabolites ◽  
Obstructive Azoospermia ◽  
Male Contraceptive ◽  
Nmr Study ◽  
Significant Difference ◽  
Styrene Maleic Anhydride ◽  
Prostatic Diseases

Nuclear magnetic resonance (NMR) spectroscopy was used to quantify citrate, glucose, lactate, glycerophosphorylcholine and choline in seminal plasma from subjects injected with a new male contraceptive RISUG, a copolymer of styrene maleic anhydride dissolved in dimethyl sulphoxide, and in seminal plasma from normal ejaculates. No significant difference in the concentration of citrate was observed between the groups, indicating that the prostate is not affected by the contraceptive. The concentrations of glucose, lactate, glycerophosphorylcholine and choline were significantly lower (P < 0.01) in subjects injected with RISUG compared with controls. In addition, metabolite ratios such as choline:citrate, citrate:lactate, choline:lactate and glycerophosphorylcholine:choline were calculated. Citrate:lactate and glycerophosphorylcholine:choline ratios were significantly lower in RISUG-injected subjects than in controls (P < 0.01), thereby indicating the occurrence of partial obstructive azoospermia. The most important finding of the present study was that the intervention of RISUG in the vas deferens even for a period as long as 8 years is absolutely safe and does not lead to prostatic diseases.

Spermatozoa and seminal plasma small extracellular vesicles miRNAs as biomarkers of boar semen cryotolerance

2021 ◽  
Author(s):  
Ana Carolina Pedrosa ◽  
Mariana A. Torres ◽  
Diego V. Alkmin ◽  
Jorge E.P. Pinzon ◽  
Simone Maria M.K. Martins ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Seminal Plasma ◽  
Boar Semen

scholarly journals Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing1,2

2016 ◽  
Vol 94 (5) ◽  
pp. 1906-1912 ◽  
Author(s):  
M. A. Torres ◽  
G. M. Ravagnani ◽  
D. F. Leal ◽  
S. M. M. K. Martins ◽  
B. B. D. Muro ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Boar Sperm ◽  
Sperm Membrane ◽  
The Stability

Seasonal changes of steroid concentrations in seminal plasma of a European wild boar

1984 ◽  
Vol 107 (3) ◽  
pp. 425-427 ◽  
Author(s):  
D. Schopper ◽  
J. Gaus ◽  
R. Claus ◽  
H. Bader
Keyword(s):  
Wild Boar ◽  
Seminal Plasma ◽  
Seasonal Pattern ◽  
Plasma Concentrations ◽  
Boar Taint ◽  
Steroid Production ◽  
European Wild Boar ◽  
Clear Seasonal Pattern ◽  
Boar Semen ◽  
Low Levels

Abstract. The influence of season on testicular steroid production as a parameter of testicular function has been studied in a wild boar. Semen was collected once weekly while it served the dummy. In seminal plasma concentrations of the following steroids were determined by radioimmunoassay: unconjugated testosterone, conjugated testosterone, unconjugated total oestrogens, conjugated total oestrogens and 5α-androst-16-en-3-one ('boar-taint steroid'). All steroids showed a clear seasonal pattern with highest concentrations in autumn and early winter and low levels from January to July. Maxima during the rutting season were 10–25 times greater than average values out of season. During a 2-month-period (mid-July until mid-September) libido was abolished and the wild boar refused to mount the dummy. These results indicate that the seasonal variation in testicular steroid production by the wild boar, regulated by photoperiod, are similar to those of the domestic boar.

Absence of seminal plasma from sperm-rich fraction decreases boar sperm quality characteristics during the course of liquid storage

2018 ◽  
Vol 198 ◽  
pp. 20-26 ◽  
Author(s):  
D.F. Leal ◽  
M.A. Torres ◽  
G.M. Ravagnani ◽  
S.M.M.K. Martins ◽  
F.V. Meirelles ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Quality ◽  
Quality Characteristics ◽  
Liquid Storage ◽  
Boar Sperm
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