scholarly journals Abnormal Prothrombin (PIVKA-II) Expression in Canine Tissues as an Indicator of Anticoagulant Poisoning

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2612
Author(s):  
Lorella Maniscalco ◽  
Katia Varello ◽  
Simona Zoppi ◽  
Giuseppina Abbamonte ◽  
Marta Ferrero ◽  
...  
Keyword(s):  
Veterinary Medicine ◽  
Histological Examination ◽  
Vitamin K ◽  
Clinical Condition ◽  
Forensic Pathology ◽  
Causes Of Death ◽  
Coagulation Disorders ◽  
Immunohistochemical Expression ◽  
Neoplastic Lesions ◽  
Aberrant Form

PIVKA-II is an aberrant form of vitamin K that has been demonstrated to be increased in human coagulation disorders and in some neoplastic diseases. In veterinary medicine, PIVKA-II levels have been demonstrated to be useful for distinguishing anticoagulant poisoning from other coagulopathies. In forensic pathology, there is the need to distinguish malicious poisoning from other causes of death and, in some cases, identifying poisoned dogs from dogs that died as a result of other coagulative disorders can be challenging. In this study, dogs that suddenly died underwent necropsy, histological examination, and toxicological analysis to establish cause of death. PIVKA-II immunohistochemical expression was evaluated on hepatic and renal tissues, and on neoplastic lesions when present. A total of 61 dogs were analyzed and anticoagulant substances were identified in 16 of the 61. Immunolabelling for PIVKA-II was observed in 27 of 61 cases in the liver and in 24 of 61 cases in the kidneys. Among the poisoned dogs, the PIVKA-II expression was present in the liver in 15 of 16 cases and in the kidneys in 16 of 16. Neoplastic lesions represented mainly by haemangiosarcomas were negative. This study highlights how the immunohistochemical expression of PIVKA-II in hepatic and renal tissues can be useful to identify patients with coagulative disorders due to clinical condition or the ingestion of anticoagulants substances.

scholarly journals Metastatic mast cell tumour in African hedgehog: A case report

2020 ◽  
Vol 65 (No. 8) ◽  
pp. 371-376
Author(s):  
M Kandefer-Gola ◽  
R Ciaputa ◽  
K Sulima ◽  
M Miszczak ◽  
S Lachowska ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Lymph Nodes ◽  
Clinical Condition ◽  
Cell Tumour ◽  
Mesenteric Lymph Nodes ◽  
Mast Cell Tumour ◽  
Tumour Metastasis ◽  
Mesenteric Lymph ◽  
Neoplastic Lesions

Neoplastic lesions are common in African hedgehogs (Atelerix albiventris). To our knowledge, so far mast cell tumour metastases have only been described in local lymph nodes. An African hedgehog was referred to the veterinary clinic. A nodule was located on the right part of the mandible. Based on the clinical trial, an abscess was suspected. Surgery was performed, but a histopathological examination was not undertaken. After few months, tumour recurrence was detected and, additionally, a second tumour was found on the metatarsal skin. Due to the deteriorating clinical condition of the patient, euthanasia was decided, and a post-mortem examination was performed. During the post-mortem examination, a spleen tumour was detected, and, furthermore, mesenteric lymph nodes, liver and lung sections were also collected. The histopathological examination confirmed mast cell tumour metastasis to the spleen and lymph nodes, additionally single mast cells were found in the liver. Interestingly, the histological picture of the tumour does not show high malignancy. To our knowledge, this is the first description of a mast cell tumour with metastases to the spleen and lymph in the African hedgehog.

Differential Immunohistochemical Expression of CD44s, E-Cadherin and �-Catenin among Hyperplastic and Neoplastic Lesions of the Prostate Gland

2013 ◽  
Vol 90 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Paraskevi Lazari ◽  
Hercules Poulias ◽  
Hariklia Gakiopoulou ◽  
Geogia-Heleni Thomopoulou ◽  
Calypso Barbatis ◽  
...  
Keyword(s):  
Prostate Gland ◽  
Immunohistochemical Expression ◽  
Neoplastic Lesions ◽  
E Cadherin

An unusual pedestrian road trauma: From forensic pathology to forensic veterinary medicine

2014 ◽  
Vol 234 ◽  
pp. e1-e4 ◽  
Author(s):  
Isabella Aquila ◽  
Ciro Di Nunzio ◽  
Orlando Paciello ◽  
Domenico Britti ◽  
Francesca Pepe ◽  
...  
Keyword(s):  
Veterinary Medicine ◽  
Forensic Pathology

scholarly journals Coagulation disorders during treatment with cefazolin and rifampicin: rare but dangerous

2021 ◽  
Vol 6 (5) ◽  
pp. 131-134
Author(s):  
Ines Kouki ◽  
Clémence Montagner ◽  
Wladimir Mauhin ◽  
Jonathan London ◽  
Thierry Lazard ◽  
...  
Keyword(s):  
Prothrombin Time ◽  
Vitamin K ◽  
Clotting Factor ◽  
Severe Bleeding ◽  
Coagulation Disorders ◽  
Severe Vitamin ◽  
Digestive Hemorrhage ◽  
Factor Deficiency

Abstract. We describe a 79-year-old man with spondylodiscitis and unknown pathogen, treated with cefazolin and rifampicin. He developed a massive digestive hemorrhage. Prothrombin time was prolonged with severe vitamin-K-dependent clotting-factor deficiency. Severe bleeding can occur during cefazolin and rifampicin use. This deficiency should be assessed before prescribing cefazolin–rifampicin and prothrombin time monitored.

scholarly journals STROKE – CORRELATIONS BETWEEN CLINICAL EXAMINATION, IMAGING FINDINGS AND MICROSCOPICAL ASPECTS

2015 ◽  
Vol 14 (3) ◽  
pp. 150-157
Author(s):  
Anca Hancu ◽  
◽  
Mariana Deacu ◽  
Madalina Bosoteanu ◽  
◽  
...  
Keyword(s):  
Histological Examination ◽  
Clinical Examination ◽  
Vessel Wall ◽  
Causes Of Death ◽  
Imaging Techniques ◽  
Pathological Process ◽  
Neurologic Diseases ◽  
Death Cases ◽  
The Brain

Stroke is the most frequent cause of disability among the neurologic diseases of the adult. In the last two decades, the extraordinary development of brain imaging techniques allows the rapid recognition of normal, ischemic, infarction or hemorrhagic brain tissue. The term cerebrovascular disease designates any abnormality of the brain resulting from a pathological process of the blood vessels, including occlusion by embolus or thrombus, rupture of a vessel, an altered permeability of the vessel wall, or increased viscosity or other changes in quality of the blood flowing through the cerebral vessels. The clinical, imagistic and histological correlations in the study of arteriosclerosis are very important due to the fact that cardiovascular disease and stroke are the main causes of death in developped countries. Our study consists of a group of 36 patients admitted in the Neurology department of Constanta County Emergency Hospital in the first six months of 2012 with the diagnosis of acute stroke, which died within the first 5 days from admission. The studied group was divided into four subgroups, according to the CT scan findings and clinical examination on admission and the autopsy results. In the sudden death cases that had no acute lesions on the brain CT on admission, histological examination revealed the diagnosis. In all cases, histological examination included in showed atherosclerotic modifications of cerebral and peripheral vessels (the circle of Willis arteries, coronary arteries, aorta and renal arteries).

scholarly journals Evaluation of Region of Interest Digital Cytology Compared to Light Microscopy for Veterinary Medicine

2019 ◽  
Vol 56 (5) ◽  
pp. 725-731 ◽  
Author(s):  
Conor J. K. Blanchet ◽  
Eric J. Fish ◽  
Amy G. Miller ◽  
Laura A. Snyder ◽  
Julia D. Labadie ◽  
...  
Keyword(s):  
Veterinary Medicine ◽  
Light Microscopy ◽  
Region Of Interest ◽  
Primary Diagnosis ◽  
General Category ◽  
Tumor Type ◽  
Research And Teaching ◽  
Neoplastic Lesions ◽  
Malignant Lesions ◽  
Small File

Digital microscopy (DM) has been employed for primary diagnosis in human medicine and for research and teaching applications in veterinary medicine, but there are few veterinary DM validation studies. Region of interest (ROI) digital cytology is a subset of DM that uses image-stitching software to create a low-magnification image of a slide, then selected ROI at higher magnification, and stitches the images into a relatively small file of the embedded magnifications. This study evaluated the concordance of ROI-DM compared to traditional light microscopy (LM) between 2 blinded clinical pathologists. Sixty canine and feline cytology samples from a variety of anatomic sites, including 31 cases of malignant neoplasia, 15 cases of hyperplastic or benign neoplastic lesions, and 14 infectious/inflammatory lesions, were evaluated. Two separate nonblinded adjudicating clinical pathologists evaluated the reports and diagnoses and scored each paired case as fully concordant, partially concordant, or discordant. The average overall concordance (full and partial concordance) for both pathologists was 92%. Full concordance was significantly higher for malignant lesions than benign. For the 40 neoplastic lesions, ROI-DM and LM agreed on general category of tumor type in 78 of 80 cases (98%). ROI-DM cytology showed robust concordance with the current gold standard of LM cytology and is potentially a viable alternative to current LM cytology techniques.

scholarly journals Макроскопічні зміни у цуценят за експериментального відтворення коронавірусного ентериту

2018 ◽  
pp. 174-177
Author(s):  
М. Л. Радзиховський
Keyword(s):  
Spinal Cord ◽  
Veterinary Medicine ◽  
Histological Examination ◽  
Internal Organs ◽  
Experimental Reproduction ◽  
Literary Sources ◽  
Significant Damage ◽  
Viral Enteritis ◽  
Brain And Spinal Cord ◽  
The Brain

На основі проведеного нами аналізу літературних джерел, результатів моніторингових та власних досліджень з’ясовано, що вірусні ентерити займають провідне місце в інфекційній патології собак і наносять значні збитки власникам тварин. Враховуючи актуальність даного питання, результатом наших досліджень є уточнення, доповнення та узагальнення даних щодо патоморфології різних органів собак за коронавірусного ентериту, що дасть можливість більш детально з’ясувати вплив збудника хвороби на організм тварин. У статті представлено результати вивчення макроскопічних змін у собак за експериментального відтворення коронавірусного ентериту. За результатами патолого-анатомічного розтину трупів цуценят спостерігаються мікроскопічні зміни у внутрішніх органах, а саме серозний лімфаденіт, тимусі та підшлунковій залозі набряк і гіперемію, нирки нерівномірно забарвлені в коричневий колір, печінка і серце збільшені, розширені судини головного і спинного мозку. Таким чином, виявлений нами комплекс патологоанатомічних змін у цуценят за експериментального відтворення хвороби можна вважати характерним критерієм патоморфологічної діагностики коронавірусного ентериту у собак. On the basis of our analysis of literary sources, the results of monitoring and our own research, it was found out that viral enteritis occupies a leading place in the infectious pathology of dogs and causes significant damage to animal owners. Taking into account the relevance of this issue, the result of our research is specification, supplement and generalization of the data on the pathomorphology of various organs of dogs for coronaviral enteritis, which will give an opportunity to more precisely determine the effect of the pathogen on the animal's body. The purpose of this work was to study and characterize the pathomorphological changes in the large intestine in experimental contamination of dogs with coronavirus enteritis. The work was carried out at the Faculty of Veterinary Medicine of Zhytomyr National Agroecological University (ZNAEU). Thanatopsy of the animals was carried out in the special laboratory of the Department of Anatomy and Histology. The article presents the results of the study of macroscopic changes in dogs in experimental reproduction of coronaviral enteritis. According to the results of the pathological anatomical section of the corpses of the puppies, microscopic changes in the internal organs are observed, namely, serous lymphadenitis, thymus and pancreas, edema and hyperemia, the kidneys are irregularly brown-colored, liver, heart as well as vessels of the brain and spinal cord are enlarged. Thus, our complex of pathoanatomical changes in puppies in the experimental reproduction of the disease has been identified by us as a characteristic criterion for the pathomorphological diagnosis of coronaviral enteritis in dogs. In the future, in order to fully cover the pathomorphological picture of the disease, it is advisable to carry out histological examination of the organs and tissues in the dog-infected Coronaviridae.

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