Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles

Pikeperch Sander lucioperca is a piscivorous species considered a promising candidate for the diversification of intensive aquaculture. This study aimed to determine the effect of a sustained-release delivery system incorporating mammalian gonadotropin-releasing hormone agonist (mGnRHa) into poly(lactic-co-glycolic acid) (PLGA) microparticles on the sex steroid levels and aspects of artificial reproduction of pikeperch. Fish were divided into four groups and injected with 20 µg mGnRHa/kg, 5-day release microparticles encapsulated with 5 µg GnRHa/kg BW (PLGA 5), 20 µg GnRHa/kg (PLGA 20), or 1 mL/kg 0.9% NaCl (control). Cumulative percentage ovulation was 100% in the PLGA 5 group, significantly higher than in other tested groups. No differences among groups were observed in latency or fecundity. The level of 11-ketotestosterone (11-KT) peaked at 40 h post-injection, and was sustained during ovulation, in all treated groups. The 17β-estradiol (E2) concentration increased in the mGnRHa-only group immediately after hormone injection, while both PLGA groups showed a reduction in E2 after injection, continuing to decrease until ovulation. A low dose of mGnRHa in PLGA microparticles significantly improves induction of ovulation and results in acceptable reproductive performance, which may positively affect pikeperch production under controlled conditions.

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Individualized correction of sex steroid deficiency in early postmenopausal women with uterine leiomyoma

Obstetrics Gynecology and Reproduction ◽

10.17749/2313-7347/ob.gyn.rep.2020.161 ◽

2020 ◽

Vol 14 (4) ◽

pp. 457-468

Author(s):

M. V. Koval ◽

T. A. Oboskalova

Keyword(s):

Postmenopausal Women ◽

Uterine Leiomyoma ◽

Low Dose ◽

Resistance Index ◽

Sex Steroid ◽

Menopausal Syndrome ◽

Early Postmenopausal Women ◽

17Β Estradiol ◽

Group 2 ◽

Group 1

Aim: to develop individualized options for correcting sex steroid deficiency in early postmenopausal patients with uterine leiomyoma.Materials and Methods. 180 postmenopausal patients with uterine leiomyoma were examined. To correct menopausal manifestations, patients were prescribed: Group 1 (n = 60) – low-dose drug estradiol 1 mg/drospirenone 2 mg, Group 2 (n = 60) – ultra-low-dose drug estradiol 0.5 mg/drospirenone 0.25 mg, Group 3 (n = 60) – patients refused to take menopausal hormone therapy (MHT). Clinical and anamnestic, laboratory and instrumental examination was carried out by assessing vasomotor and genitourinary symptoms. Special attention was paid to ultrasound examination of the pelvic organs with Dopplerometry of the intratumoral vessels.Results. The vasomotor manifestations of menopausal syndrome in postmenopausal patients with uterine leiomyoma were effectively relieved with drug containing 17β-estradiol/drospirenone, regardless of component dosage (RR = 0.25). The size of nodes in uterine leiomyoma type 2–6 according to classification of The International Federation of Obstetricians and Gynecologists (FIGO) as well as Dopplerometry data in the uterine artery in postmenopausal women showed no changes after 9-month use of 17β-estradiol/drospirenone. In Group 1, increased vascularization intensity (RR = 1.4) and decreased peripheral vascular resistance in intranodular vessels were revealed in 6 % patients.Conclusion. The use of MHT drugs at varying component doses did not enlarge size of leiomyoma nodes nor affected hemodynamic parameters in the uterine arteries during 6-month-treatment. However, while prolonging MHT duration, it increased vascularization of myoma nodes assessed by measuring intranodular blood flow, increased vascularization and decreased resistance index after administering low-dose vs. ultra-low-dose MHT therapy that should be taken into account upon planning therapeutic regimen and its timeframe.

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Effect of raloxifene and low-dose percutaneous 17β-estradiol on menopause symptoms and endometrium—A randomized controlled trial

Maturitas ◽

10.1016/j.maturitas.2008.10.013 ◽

2009 ◽

Vol 62 (1) ◽

pp. 81-84 ◽

Cited By ~ 9

Author(s):

Beatriz Valiati ◽

Edison Capp ◽

Maria Isabel Edelweiss ◽

Fernando Monteiro de Freitas ◽

Maria Celeste Osório Wender

Keyword(s):

Randomized Controlled Trial ◽

Low Dose ◽

Controlled Trial ◽

Randomized Controlled ◽

17Β Estradiol

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Cortical reaction as an egg quality indicator in artificial reproduction of pikeperch, Sander lucioperca

Reproduction Fertility and Development ◽

10.1071/rd11264 ◽

2012 ◽

Vol 24 (6) ◽

pp. 843 ◽

Cited By ~ 16

Author(s):

Daniel Żarski ◽

Sławomir Krejszeff ◽

Katarzyna Palińska ◽

Katarzyna Targońska ◽

Krzysztof Kupren ◽

...

Keyword(s):

Quality Evaluation ◽

Egg Quality ◽

Effect Of Temperature ◽

Artificial Reproduction ◽

Female Fish ◽

Sander Lucioperca ◽

Embryo Survival ◽

Hormonal Stimulation ◽

Cortical Reaction

The aim of this study was to investigate the process of the cortical reaction in eggs of pikeperch, Sander lucioperca (L.), as well as the application of microscopic assessment of this process in egg quality evaluation. The analysis was carried out with eggs obtained from 10 females by artificial reproduction, in which hormonal stimulation with hCG was applied. Subsequently, each sample of eggs (separately from each female fish) was analysed. The analysis included observation of the cortical reaction and the process of egg swelling, and determination of the effect of temperature (12, 14 and 16°C) and the presence of spermatozoa on the cortical reaction. The results indicate that the cortical reaction in pikeperch eggs is quite violent, resulting in visible deformation of eggs between 3 and 5 min after activation. No effect of temperature or the presence of spermatozoa on the cortical reaction was observed. A strong correlation was recorded for the percentage of egg deformations observed and embryo survival rate. The described method of determination of pikeperch egg quality (based on egg deformation rate between 3 and 5 min after activation) may be highly useful, both in scientific research (where high-quality eggs are required) and in hatchery practice.

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Effects of SCH-23390 and Sulpiride on Active Avoidance in Ovariectomized Rats Treated with a Low Dose of 17β-Estradiol

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-014-2407-2 ◽

2014 ◽

Vol 156 (5) ◽

pp. 612-614 ◽

Cited By ~ 2

Author(s):

Yu. O. Fedotova ◽

N. S. Sapronov

Keyword(s):

Active Avoidance ◽

Low Dose ◽

Sch 23390 ◽

Ovariectomized Rats ◽

17Β Estradiol

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Severe hyperpigmentation and scarring following glycolic acid peel treatment in combination with low-dose isotretinoin

European Journal of Medical Research ◽

10.1186/s40001-014-0060-x ◽

2014 ◽

Vol 19 (1) ◽

Cited By ~ 7

Author(s):

Peter Arne Gerber ◽

Gabriela Kukova ◽

Edwin Bölke ◽

Bernhard Homey ◽

Evelyn Diedrichson

Keyword(s):

Glycolic Acid ◽

Low Dose

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Trimester-specific, gender-specific, and low-dose effects associated with non-monotonic relationships of bisphenol A on estrone, 17β-estradiol and estriol

Environment International ◽

10.1016/j.envint.2019.105304 ◽

2020 ◽

Vol 134 ◽

pp. 105304 ◽

Cited By ~ 2

Author(s):

Jiufeng Li ◽

Wenxin Zhang ◽

Hongzhi Zhao ◽

Yanqiu Zhou ◽

Shunqing Xu ◽

...

Keyword(s):

Bisphenol A ◽

Low Dose ◽

Dose Effects ◽

17Β Estradiol ◽

Gender Specific ◽

Low Dose Effects

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Management of Puberty in Constitutional Delay of Growth and Puberty

Journal of Pediatric Endocrinology and Metabolism ◽

10.1515/jpem-2001-s207 ◽

2001 ◽

Vol 14 (s2) ◽

Cited By ~ 10

Author(s):

F. De Luca ◽

J. Argente ◽

L. Cavallo ◽

E. Crowne ◽

H.A. Delemarre-Van de Waal ◽

...

Keyword(s):

Social Relationships ◽

School Performance ◽

Low Dose ◽

Well Being ◽

Growth Potential ◽

Sex Steroid ◽

Optimal Timing ◽

Short Course ◽

Constitutional Delay

AbstractConstitutional delay of growth and puberty (CDGP) is the most common presenting form of short stature, but no single test can infallibly discriminate CDGP and isolated hypogonado- trophic hypogonadism. Management of puberty in CDGP aims to optimise not only growth - maintaining body proportions and improving peak bone mass without impairing growth potential - but also well-being; for example, the distress boys often suffer because of their lack of growth and pubertal progression can affect their school performance and social relationships. Typical sex steroid treatments to induce puberty in boys with CDGP include testosterone (T) enanthate, T undecanoate, mixed T esters, T transdermal patches, and oxandrolone p.o. Compared with other regimens, short-course low-dose depot T i.m. is an effective, practical, safe, well tolerated, and inexpensive regimen. Some unresolved problems in management include optimal timing and dose of sex steroid treatment, the role of GH in CDGP, and the management of CDGP in girls.

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Glyphosate-based herbicide enhances the uterine sensitivity to estradiol in rats

Journal of Endocrinology ◽

10.1530/joe-18-0207 ◽

2018 ◽

Vol 239 (2) ◽

pp. 197-213 ◽

Cited By ~ 9

Author(s):

Marlise Guerrero Schimpf ◽

María M Milesi ◽

Enrique H Luque ◽

Jorgelina Varayoud

Keyword(s):

Cell Proliferation ◽

Endometrial Hyperplasia ◽

Low Dose ◽

Saline Solution ◽

Female Rats ◽

Postnatal Exposure ◽

Rat Uterus ◽

Mrna Extraction ◽

17Β Estradiol ◽

Prepubertal Rats

In a previous work, we detected that postnatal exposure to a glyphosate-based herbicide (GBH) alters uterine development in prepubertal rats causing endometrial hyperplasia and increasing cell proliferation. Our goal was to determine whether exposure to low dose of a GBH during postnatal development might enhance the sensitivity of the uterus to an estrogenic treatment. Female Wistar pups were subcutaneously injected with saline solution (control) or GBH using the reference dose (2 mg/kg/day, EPA) on postnatal days (PND) 1, 3, 5 and 7. At weaning (PND21), female rats were bilaterally ovariectomized and treated with silastic capsules containing 17β-estradiol (E2, 1 mg/mL) until they were 2 months of age. On PND60, uterine samples were removed and processed for histology, immunohistochemistry and mRNA extraction to evaluate: (i) uterine morphology, (ii) uterine cell proliferation by the detection of Ki67, (iii) the expression of the estrogen receptors alpha (ESR1) and beta (ESR2) and (iv) the expression of WNT7A and CTNNB1. GBH-exposed animals showed increased luminal epithelial height and stromal nuclei density. The luminal and glandular epithelium were markedly hyperplastic in 43% of GBH-exposed animals. GBH exposure caused an increase in E2-induced cell proliferation in association with an induction of both ESR1 and ESR2. GBH treatment decreased membranous and cytoplasmic expression of CTNNB1 in luminal and glandular epithelial cells and increased WNT7A expression in the luminal epithelium. These results suggest that early postnatal exposure to a GBH enhances the sensitivity of the rat uterus to estradiol and induces histomorphological and molecular changes associated with uterine hyperplasia.

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