scholarly journals Performance Efficiency of Conventional Treatment Plants and Constructed Wetlands towards Reduction of Antibiotic Resistance

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 114
Author(s):  
Moushumi Hazra ◽  
Lisa M. Durso
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Constructed Wetlands ◽  
Low Income ◽  
Conventional Treatment ◽  
Animal Health ◽  
Control Point ◽  
Antibiotic Resistant ◽  
Critical Control Point ◽  
Entry Points

Domestic and industrial wastewater discharges harbor rich bacterial communities, including both pathogenic and commensal organisms that are antibiotic-resistant (AR). AR pathogens pose a potential threat to human and animal health. In wastewater treatment plants (WWTP), bacteria encounter environments suitable for horizontal gene transfer, providing an opportunity for bacterial cells to acquire new antibiotic-resistant genes. With many entry points to environmental components, especially water and soil, WWTPs are considered a critical control point for antibiotic resistance. The primary and secondary units of conventional WWTPs are not designed for the reduction of resistant microbes. Constructed wetlands (CWs) are viable wastewater treatment options with the potential for mitigating AR bacteria, their genes, pathogens, and general pollutants. Encouraging performance for the removal of AR (2–4 logs) has highlighted the applicability of CW on fields. Their low cost of construction, operation and maintenance makes them well suited for applications across the globe, especially in developing and low-income countries. The present review highlights a better understanding of the performance efficiency of conventional treatment plants and CWs for the elimination/reduction of AR from wastewater. They are viable alternatives that can be used for secondary/tertiary treatment or effluent polishing in combination with WWTP or in a decentralized manner.

scholarly journals Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

2019 ◽  
Author(s):  
Cian Smyth ◽  
Aidan O’Flaherty ◽  
Fiona Walsh ◽  
Thi Thuy Do
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Control Point ◽  
Treated Wastewater ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Faecal Coliforms ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Extended Spectrum

AbstractWastewater treatment plants (WWTPs) provide optimal conditions for the maintenance and spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this work we describe the occurrence of antibiotic resistant faecal coliforms and their mechanisms of antibiotic resistance in the effluent of two urban WWTPs in Ireland. Effluent samples were collected from two WWTPs in Spring and Autumn of 2015 and 2016. The bacterial susceptibility patterns to 13 antibiotics were determined. The phenotypic tests were carried out to identify AmpC or extended-spectrum β-lactamase (ESBL) producers. The presence of ESBL genes were detected by PCR. Plasmids carrying ESBL genes were transformed into Escherichia coli DH5α recipient and underwent plasmid replicon typing to identify incompatibility groups. More than 90% of isolated faecal coliforms were resistant to amoxicillin and ampicillin, followed by tetracycline (up to 39.82%), ciprofloxacin (up to 31.42%) and trimethoprim (up to 37.61%). Faecal coliforms resistant to colistin and imipenem were detected in all effluent samples. Up to 53.98% of isolated faecal coliforms expressed a multi-drug resistance (MRD) phenotype. AmpC production was confirmed in 5.22% of isolates. The ESBL genes were confirmed for 11.84% of isolates (9.2% of isolates carried blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15). Plasmids extracted from 52 ESBL isolates were successfully transformed into recipient E. coli. The detected plasmid incompatibility groups included the IncF group, IncI1, IncHI1/2 and IncA/C. These results provide evidence that treated wastewater is polluted with ARB and MDR faecal coliforms and are sources of ESBL-producing, carbapenem and colistin resistant Enterobacteriaceae.ImportanceAntibiotic resistant bacteria (ARB) are an emerging environmental concern with a potential impact on human health. The results provide the evidence that treated wastewater is polluted with antibiotic resistant bacteria containing mobile resistance mechanisms of importance to clinical treatment of pathogens and multi-drug resistant (MDR) faecal coliforms. They are sources of relatively high proportions of ESBL-producing Enterobacteriaceae, and include carbapenem and colistin resistant Enterobacteriaceae. The significance of this study is the identification of the role of WWTPs as a potential control point to reduce or stop the movement of ESBL, MDR and colistin resistant bacteria into the environment from further upstream sources, such as human or animal waste.

scholarly journals Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

2019 ◽  
Author(s):  
Cian Smyth ◽  
Aidan O’Flaherty ◽  
Fiona Walsh ◽  
Thi Thuy Do
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Control Point ◽  
Treated Wastewater ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Faecal Coliforms ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Extended Spectrum

AbstractWastewater treatment plants (WWTPs) provide optimal conditions for the maintenance and spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this work we describe the occurrence of antibiotic resistant faecal coliforms and their mechanisms of antibiotic resistance in the effluent of two urban WWTPs in Ireland. Effluent samples were collected from two WWTPs in Spring and Autumn of 2015 and 2016. The bacterial susceptibility patterns to 13 antibiotics were determined. The phenotypic tests were carried out to identify AmpC or extended-spectrum β-lactamase (ESBL) producers. The presence of ESBL genes were detected by PCR. Plasmids carrying ESBL genes were transformed into Escherichia coli DH5α recipient and underwent plasmid replicon typing to identify incompatibility groups. More than 90% of isolated faecal coliforms were resistant to amoxicillin and ampicillin, followed by tetracycline (up to 39.82%), ciprofloxacin (up to 31.42%) and trimethoprim (up to 37.61%). Faecal coliforms resistant to colistin and imipenem were detected in all effluent samples. Up to 53.98% of isolated faecal coliforms expressed a multi-drug resistance (MRD) phenotype. AmpC production was confirmed in 5.22% of isolates. The ESBL genes were confirmed for 11.84% of isolates (9.2% of isolates carried blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15). Plasmids extracted from 52 ESBL isolates were successfully transformed into recipient E. coli. The detected plasmid incompatibility groups included the IncF group, IncI1, IncHI1/2 and IncA/C. These results provide evidence that treated wastewater is polluted with ARB and MDR faecal coliforms and are sources of ESBL-producing, carbapenem and colistin resistant Enterobacteriaceae.ImportanceAntibiotic resistant bacteria (ARB) are an emerging environmental concern with a potential impact on human health. The results provide the evidence that treated wastewater is polluted with antibiotic resistant bacteria containing mobile resistance mechanisms of importance to clinical treatment of pathogens and multi-drug resistant (MDR) faecal coliforms. They are sources of relatively high proportions of ESBL-producing Enterobacteriaceae, and include carbapenem and colistin resistant Enterobacteriaceae. The significance of this study is the identification of the role of WWTPs as a potential control point to reduce or stop the movement of ESBL, MDR and colistin resistant bacteria into the environment from further upstream sources, such as human or animal waste.

scholarly journals Technologies in Wastewater Treatment Plants for the Removal of Antibiotics, Resistant Bacteria and Antibiotic Resistance Genes: a Review of the Current Literature

2021 ◽  
Vol 26 ◽  
Author(s):  
Maria Camila Zapata Zúñiga ◽  
Miguel Angel Parra-Pérez ◽  
Johan Alexander Álvarez-Berrio ◽  
Nidia Isabel Molina-Gómez
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Resistant Bacteria ◽  
Sources Of Information ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Treatment Processes

This study aimed to evaluate the efficiency of technologies for removing antibiotics, antibiotic-resistant bacteria and their antibiotic resistance genes, and the countries where they have been developed. For this purpose, was conducted a systematic review to identify the tertiary treatments to remove the above-mentioned pollutants. The ScienceDirect and Scopus databases were used as sources of information, taking into account only experimental research from 2006 to 2019 and technologies with removal rates higher than 70% to the information analyses. From the analysis of 9 technologies evaluated, in a set of 47 investigations, photo-Fenton, and electrochemical treatments were found to be the most efficient in the removal of antibiotics; gamma radiation and photocatalysis with TiO2 and UV revealed better results in the removal of resistant microbial agents and their resistance genes, with efficiencies of 99.9%. As one of the largest producers and consumers of antibiotics, China appears to be the country with the most scientific research on the area. The importance of innovation in wastewater treatment processes to achieve better results in the remotion of antibiotics, antibiotic-resistant bacteria, and their resistance genes is highlighted, given the effects on the aquatic ecosystems and public health.

scholarly journals Genome Characterization of the Novel Lytic Genome Sequence of Phage YUEEL01 of the Myoviridae Family

2021 ◽  
Author(s):  
Gurusamy Raman ◽  
Ayyaru Sivasankaran ◽  
SeonJoo Park ◽  
Young-Ho Ahn
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Animal Health ◽  
Gc Content ◽  
Phenotypic Characterization ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Urban Hospital ◽  
Protein Coding ◽  
Antibiotic Resistant

Abstract Antimicrobial resistance is a global concern due to its rapid emergence in the environment and the associated high risk to human and animal health. Municipal wastewater, including urban, hospital, and pharmaceutical effluent, is the main source of antibiotic and antibiotic resistant bacteria (ARB) contamination, and biological processes are commonly used for wastewater treatment. Biologically based strategies seem to be a promising approach to effective integrated ARB control since they can focus on the bases of antibiotic resistance. To develop an effective bacteriophage against multi-drug resistance (MDR) microbes in municipal wastewater, phage YUEEL01 was isolated from a livestock farm, and its genome sequenced. Phenotypic characterization suggested that the phage is a member of the Myoviridae family, and that it encodes 168,266 bp with an overall GC content of 35.4% had 259 putative protein-coding genes, 11 tRNA and six hypothetical genes. A number of putative DNA replication and regulation, DNA packing and structure and host lysis genes were identified. Further, whole genome phylogenetic analysis demonstrated that phage YUEEL01 is closely related to phage slur03. Elucidation of the YUEEL01 phage might be helpful when developing antibacterial tools for controlling MDR bacteria in wastewater treatment systems.

scholarly journals Antibiotic-Resistant Escherichia coli in Wastewaters, Surface Waters, and Oysters from an Urban Riverine System

2007 ◽  
Vol 73 (17) ◽  
pp. 5667-5670 ◽  
Author(s):  
A. J. Watkinson ◽  
G. B. Micalizzi ◽  
G. M. Graham ◽  
J. B. Bates ◽  
S. D. Costanzo
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Surface Water ◽  
Surface Waters ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Antibiotic Resistant

ABSTRACT The antibiotic resistance (AR) patterns of 462 Escherichia coli isolates from wastewater, surface waters, and oysters were determined. Rates of AR and multiple-AR among isolates from surface water sites adjacent to wastewater treatment plant (WWTP) discharge sites were significantly higher (P < 0.05) than those among other isolates, whereas the rate of AR among isolates from oysters exposed to WWTP discharges was low (<10%).

scholarly journals Antibiotic Resistance: One Health One World Outlook

2021 ◽  
Vol 11 ◽  
Author(s):  
Bilal Aslam ◽  
Mohsin Khurshid ◽  
Muhammad Imran Arshad ◽  
Saima Muzammil ◽  
Maria Rasool ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Global Health ◽  
One Health ◽  
Animal Health ◽  
Significant Risk ◽  
World Health ◽  
Health Concern ◽  
Antibiotic Resistant ◽  
Human Animal ◽  
The One

Antibiotic resistance (ABR) is a growing public health concern worldwide, and it is now regarded as a critical One Health issue. One Health’s interconnected domains contribute to the emergence, evolution, and spread of antibiotic-resistant microorganisms on a local and global scale, which is a significant risk factor for global health. The persistence and spread of resistant microbial species, and the association of determinants at the human-animal-environment interface can alter microbial genomes, resulting in resistant superbugs in various niches. ABR is motivated by a well-established link between three domains: human, animal, and environmental health. As a result, addressing ABR through the One Health approach makes sense. Several countries have implemented national action plans based on the One Health approach to combat antibiotic-resistant microbes, following the Tripartite’s Commitment Food and Agriculture Organization (FAO)-World Organization for Animal Health (OIE)-World Health Organization (WHO) guidelines. The ABR has been identified as a global health concern, and efforts are being made to mitigate this global health threat. To summarize, global interdisciplinary and unified approaches based on One Health principles are required to limit the ABR dissemination cycle, raise awareness and education about antibiotic use, and promote policy, advocacy, and antimicrobial stewardship.

scholarly journals Do wastewater treatment plants increase antibiotic resistant bacteria or genes in the environment? Protocol for a systematic review

2019 ◽  
Author(s):  
Daloha Rodríguez-Molina ◽  
Petra Mang ◽  
Heike Schmitt ◽  
Mariana Carmen Chifiriuc ◽  
Katja Radon ◽  
...  
Keyword(s):  
Systematic Review ◽  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Water Samples ◽  
Wastewater Treatment Plants ◽  
Resistant Bacteria ◽  
Process Data ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Antimicrobial Resistance Genes

Background. Antibiotic resistance is a global public health threat. Water from human activities is collected at wastewater treatment plants where processes often do not sufficiently neutralize antibiotic resistant bacteria and genes, which are further shed into the local environment. This protocol outlines the steps to conduct a systematic review based on the Population, Exposure, Comparator and Outcome (PECO) framework, aiming at answering the question Are antimicrobial-resistant enterobacteriaceae and antimicrobial resistance genes present (O) in air and water samples (P) taken either near or downstream or downwind or down-gradient from wastewater treatment plants (E), as compared to air and water samples taken either further away or upstream or upwind or up-gradient from such wastewater treatment plant (C)? Presence of antimicrobial-resistant bacteria and genes will be quantitatively measured by extracting their prevalence or concentration, depending on the reviewed study. Methods. We will search PubMed, EMBASE, the Cochrane database and Web of Science for original articles published from 01-Jan-2000 to 03-Sep-2018 with language restriction. Articles will undergo a relevance and a design screening process. Data from eligible articles will be extracted by two independent reviewers. Further, we will perform a risk of bias assessment using a decision matrix. We will synthesize and present results in narrative and tabular form and will perform a meta-analysis if heterogeneity of results allows it. Discussion. Antibiotic resistance in environmental samples around wastewater treatment plants may pose a risk of exposure to workers and nearby residents. Results from the systematic review outlined in this protocol will allow to estimate the extend of exposure, to inform policy making and help to design future studies.

scholarly journals Characterization and Quantitation of a Novel β-Lactamase Gene Found in a Wastewater Treatment Facility and the Surrounding Coastal Ecosystem

2011 ◽  
Vol 77 (23) ◽  
pp. 8226-8233 ◽  
Author(s):  
Miguel I. Uyaguari ◽  
Erin B. Fichot ◽  
Geoffrey I. Scott ◽  
R. Sean Norman
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Antibiotic Resistance Gene ◽  
Significant Proportion ◽  
Coastal Ecosystem ◽  
Treated Wastewater ◽  
Gene Copy ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Content Type

ABSTRACTWastewater treatment plants (WWTPs) are engineered structures that collect, concentrate, and treat human waste, ultimately releasing treated wastewater into local environments. While WWTPs efficiently remove most biosolids, it has been shown that many antibiotics and antibiotic-resistant bacteria can survive the treatment process. To determine how WWTPs influence the concentration and dissemination of antibiotic-resistant genes into the environment, a functional metagenomic approach was used to identify a novel antibiotic resistance gene within a WWTP, and quantitative PCR (qPCR) was used to determine gene copy numbers within the facility and the local coastal ecosystem. From the WWTP metagenomic library, the fosmid insert contained in one highly resistant clone (MIC, ∼416 μg ml−1ampicillin) was sequenced and annotated, revealing 33 putative genes, including a 927-bp gene that is 42% identical to a functionally characterized β-lactamase fromStaphylococcus aureusPC1. Isolation and subcloning of this gene, referred to asblaM-1, conferred ampicillin resistance to itsEscherichia colihost. When normalized to volume, qPCR showed increased concentrations ofblaM-1during initial treatment stages but 2-fold-decreased concentrations during the final treatment stage. The concentration ng−1DNA increased throughout the WWTP process from influent to effluent, suggesting thatblaM-1makes up a significant proportion of the overall genetic material being released into the coastal ecosystem. Average discharge was estimated to be 3.9 × 1014copies of theblaM-1gene released daily into this coastal ecosystem. Furthermore, the gene was observed in all sampled coastal water and sediment samples surrounding the facility. Our results suggest that WWTPs may be a pathway for the dissemination of novel antibiotic resistance genes into the environment.

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