scholarly journals Nitric Oxide as a Remedy against Oxidative Damages in Apple Seeds Undergoing Accelerated Ageing

Antioxidants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 70
Author(s):  
Katarzyna Ciacka ◽  
Marcin Tyminski ◽  
Agnieszka Gniazdowska ◽  
Urszula Krasuska
Keyword(s):  
Nitric Oxide ◽  
Oxidative Modification ◽  
Accelerated Ageing ◽  
Artificial Ageing ◽  
Enzymatic Antioxidants ◽  
High Concentration ◽  
Seed Vigour ◽  
Transcript Levels ◽  
Oxidative Damages ◽  
Apple Seeds

Seed ageing is associated with a high concentration of reactive oxygen species (ROS). Apple (Malus domestica Borkh.) seeds belong to the orthodox type. Due to a deep dormancy, they may be stored in dry condition at 5 °C for a long time, without viability loss. In the laboratory, artificial ageing of apple seeds is performed by imbibition in wet sand at warm temperature (33 °C). The aim of the work was to study nitric oxide (NO) as a seed vigour preservation agent. Embryos isolated from apple seeds subjected to accelerated ageing for 7, 14, 21 or 40 days were fumigated with NO. Embryo quality was estimated by TTC and MDA tests. ROS level was confirmed by NBT staining. We analysed the alteration in transcript levels of CAT, SOD and POX. NO fumigation of embryos of seeds aged for 21 days stimulated germination and increased ROS level which correlated to the elevated expression of RBOH. The increased total antioxidant capacity after NO fumigation was accompanied by the increased transcript levels of genes encoding enzymatic antioxidants, that could protect against ROS overaccumulation. Moreover, post-aged NO application diminished the nitro-oxidative modification of RNA, proving NO action as a remedy in oxidative remodelling after seeds ageing.

Effect of nitric oxide on Sertoli cell microtubule of piglets

2009 ◽  
Vol 6 (3) ◽  
pp. 257-263 ◽  
Author(s):  
Yang Li ◽  
Wang Xian-zhong ◽  
Yang Meng-bo ◽  
Zhang Jia-hua
Keyword(s):  
Nitric Oxide ◽  
Enzyme Activity ◽  
Protein Kinase ◽  
Cell Viability ◽  
Sodium Nitroprusside ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
High Concentration ◽  
Treatment Results ◽  
Anti Oxidant

AbstractTo illustrate the effect of nitric oxide (NO) on the microtubules of Sertoli cells (SC), SCs of piglets were treated with sodium nitroprusside (SNP). Changes in cell viability, anti-oxidant activity, enzyme activity and p38 mutagen-activated protein kinase (p38MAPK) activation were detected. The results were as follows. A low concentration of NO can keep SC microtubule and cell viability normal, and a high concentration of NO could increase p38MAPK activation, decrease anti-oxidant activity and transferrin secretion, and destroy the structure and distribution of the microtubules. The results suggest that SNP treatment results in an increase in NO in SCs and decreased cell anti-oxidant activity. The high concentration of NO destroys cell microtubules by activating p38MAPK.

Genetic dissection of seed vigour under artificial ageing conditions using two joined maize recombinant inbred line populations

2014 ◽  
Vol 133 (6) ◽  
pp. 728-737 ◽  
Author(s):  
Lixia Ku ◽  
Xinjian Cui ◽  
Fangfang Cheng ◽  
Shulei Guo ◽  
Jianshuang Qi ◽  
...  
Keyword(s):  
Inbred Line ◽  
Recombinant Inbred Line ◽  
Recombinant Inbred ◽  
Artificial Ageing ◽  
Genetic Dissection ◽  
Seed Vigour

scholarly journals SALMONELLA TYPHIMURIUM INFECTION: ANTIOXIDANT STATUS OF INFECTED POULTRY AND EFFICACY OF TREATMENT WITH KHAYA GRANDIFOLIOLA EXTRACT

2021 ◽  
Vol 10 (3) ◽  
pp. 72-79
Author(s):  
Elodie Yamako Konack ◽  
Jean Baptiste Sokoudjou ◽  
Norbert Kodjio ◽  
Gabriel Tchuente Kamsu ◽  
Huguette Bocanestine Laure Feudjio ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Biochemical Markers ◽  
Broiler Chickens ◽  
Antioxidant Status ◽  
Antioxidant Activities ◽  
The Body ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Salmonella Infections

Salmonella infections remain one of the major health problems in both poultry farming and human medicine. In addition, resistance to Salmonella has emerged as a global health problem in both sectors. The present study aimed at evaluating the in vivo antisalmonellal and antioxidant activities of 95° ethanol extract of Khaya grandifoliola using broiler chickens as animal model. Animals were divided into the normal control group, negative control group, positive control group and three test groups treated with the extract at 10, 20 and 40 mg/kg respectively. The antioxidant status of broiler chickens was also evaluated by measuring the concentration of enzymatic antioxidants (catalase, glutathione peroxidase) and biochemical markers (malondialdehyde and nitric oxide). The results showed that infected animals treated with the Khaya grandifoliola extract at 40 mg/kg recovered on day 11 after the beginning of the treatment and on day 13 for those treated at the 10 and 20 mg/kg. The antioxidant assay showed that the infection led to the reduction of enzymatic markers in the body of infected animals, while the treatment increases such makers. The infection resulted in a significant increase in serum and pulmonary malondialdehyde. It also caused a significant decrease in cardiac and pulmonary nitric oxide whereas the treatment depending on the doses of the extract tends to normalize these biochemical markers. The overall results showed that Khaya grandifoliola extract can be successfully used in the treatment of avian salmonellosis as well as the management of the oxidative stress caused by the infection.

Human placental and fetal membrane nitric oxide synthase activity before, during and after labour at term

1995 ◽  
Vol 7 (6) ◽  
pp. 1505 ◽  
Author(s):  
Iulio JL Di ◽  
NM Gude ◽  
RG King ◽  
SP Brennecke
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Caesarean Section ◽  
Normal Vaginal Delivery ◽  
Fetal Membrane ◽  
High Concentration ◽  
Experimental Conditions ◽  
Nos Inhibitor ◽  
Tissue Specimens ◽  
Oxide Synthase

The aim of this study was to determine whether any labour-associated changes in nitric oxide synthase (NOS) activity occur in human placenta and fetal membranes. NOS activity in amnion, choriodecidua, and placenta obtained from women before (at Caesarean section, not in labour), during (at Caesarean section, in labour) and after (spontaneous onset labour, normal vaginal delivery) labour was assessed by measuring conversion of radio-labelled L-arginine to L-citrulline. NOS activity, as judged by its inhibition by the specific NOS inhibitor N omega-nitro-L-arginine, was present in placental and amnionic tissues, but not in choriodecidual tissue specimens. Activity detected in choriodecidua was significantly blocked during incubation with a high concentration of valine, suggesting that L-arginine was being consumed by reactions other than NOS under the experimental conditions in that tissue. There were no significant differences among the labour groups in either amnion or placental NOS activities measured in the presence of 1 microM L-arginine. Amnion NOS activity was significantly less than that in placenta. Placental V(max) and Km values (determined after removal of endogenous L-arginine) did not differ significantly among the different labour groups.

scholarly journals A Note on the Surface Deterioration of Scrimber Composites Exposed to Artificial Ageing

Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 846
Author(s):  
Minzhen Bao ◽  
Fei Rao ◽  
Sheng He ◽  
Yongjie Bao ◽  
Zaixing Wu ◽  
...  
Keyword(s):  
Light Exposure ◽  
Surface Characteristics ◽  
Resin Content ◽  
Color Variation ◽  
Accelerated Ageing ◽  
Artificial Ageing ◽  
Surface Color ◽  
Surface Contact Angle ◽  
Xenon Light ◽  
Surface Gloss

The effect of ultraviolet radiation on the surface deterioration of scrimber composites with different densities and levels of resin content was investigated for this paper. The surface color, gloss, roughness, and wettability of the samples were investigated to determine the extent of surface changes due to accelerated ageing. The results indicated that all scrimber composites exhibited variation in the color of their surfaces during artificial xenon light exposure. After artificial accelerated ageing, the surface contact angle and roughness were found to increase, whereas the surface gloss and surface free energy decreased. Furthermore, it was observed that the density and resin content of the scrimber composites contributed to reductions in their color variation, wettability, and roughness during weathering, which suggested that the density and resin content played important roles in determining their surface photodegradation properties. Artificial ageing resulted in the degradation of lignin and hemicellulose, thus causing severe surface characteristics.

Gentamicin induced nitric oxide-related oxidative damages on vestibular afferents in the guinea pig

2006 ◽  
Vol 211 (1-2) ◽  
pp. 46-53 ◽  
Author(s):  
Sung Hwa Hong ◽  
Sook Kyung Park ◽  
Yang-Sun Cho ◽  
Hyun-Seok Lee ◽  
Ki Ryung Kim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Guinea Pig ◽  
Oxidative Damages ◽  
Vestibular Afferents

Abstract 6371: Gaseous nitric oxide at high concentration is a powerful anti-tumor agent both in-vitro and in-vivo

2020 ◽  
Author(s):  
Hila Confino ◽  
Steve Lisi ◽  
Rinat Kalaora ◽  
Matan Goldshtein ◽  
Amir Avniel
Keyword(s):  
Nitric Oxide ◽  
High Concentration ◽  
Tumor Agent

scholarly journals Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

2020 ◽  
Vol 21 (4) ◽  
pp. 1410 ◽  
Author(s):  
Sreeja Sarasamma ◽  
Gilbert Audira ◽  
Petrus Siregar ◽  
Nemi Malhotra ◽  
Yu-Heng Lai ◽  
...  
Keyword(s):  
Health Risk ◽  
Tissue Distribution ◽  
Reproductive Function ◽  
Aquatic Organisms ◽  
Behavioral Changes ◽  
High Concentration ◽  
Plastic Pollution ◽  
Oxidative Damages ◽  
New Evidence ◽  
Locomotion Activity

Plastic pollution is a growing global emergency and it could serve as a geological indicator of the Anthropocene era. Microplastics are potentially more hazardous than macroplastics, as the former can permeate biological membranes. The toxicity of microplastic exposure on humans and aquatic organisms has been documented, but the toxicity and behavioral changes of nanoplastics (NPs) in mammals are scarce. In spite of their small size, nanoplastics have an enormous surface area, which bears the potential to bind even bigger amounts of toxic compounds in comparison to microplastics. Here, we used polystyrene nanoplastics (PS-NPs) (diameter size at ~70 nm) to investigate the neurobehavioral alterations, tissue distribution, accumulation, and specific health risk of nanoplastics in adult zebrafish. The results demonstrated that PS-NPs accumulated in gonads, intestine, liver, and brain with a tissue distribution pattern that was greatly dependent on the size and shape of the NPs particle. Importantly, an analysis of multiple behavior endpoints and different biochemical biomarkers evidenced that PS-NPs exposure induced disturbance of lipid and energy metabolism as well as oxidative stress and tissue accumulation. Pronounced behavior alterations in their locomotion activity, aggressiveness, shoal formation, and predator avoidance behavior were exhibited by the high concentration of the PS-NPs group, along with the dysregulated circadian rhythm locomotion activity after its chronic exposure. Moreover, several important neurotransmitter biomarkers for neurotoxicity investigation were significantly altered after one week of PS-NPs exposure and these significant changes may indicate the potential toxicity from PS-NPs exposure. In addition, after ~1-month incubation, the fluorescence spectroscopy results revealed the accumulation and distribution of PS-NPs across zebrafish tissues, especially in gonads, which would possibly further affect fish reproductive function. Overall, our results provided new evidence for the adverse consequences of PS-NPs-induced behavioral dysregulation and changes at the molecular level that eventually reduce the survival fitness of zebrafish in the ecosystem.

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