scholarly journals Correlating Structure and Morphology of Andiroba Leaf (Carapa guianensis Aubl.) by Microscopy and Fractal Theory Analyses

2021 ◽  
Vol 11 (13) ◽  
pp. 5848
Author(s):  
Robert S. Matos ◽  
Ştefan Ţălu ◽  
Gunar V. S. Mota ◽  
Erveton P. Pinto ◽  
Marcelo A. Pires ◽  
...  

The Amazon rainforest is considered a megadiverse biome, where several species of its rich flora are still unknown. The anatomy of their leaves usually identifies species. In this paper, we present a complete characterization of the leaf surface of Amazon Carapa guianensis Aubl. (Andiroba), using microscopy and fractal theory to be considered a possible tool for investigating different leaves spatial patterns, especially in species with similar leaf architecture. The SEM results revealed the cellular structures and other non-cellular structures that make up the leaf architecture, both for the abaxial and adaxial sides. The cells responsible for the plant photosynthesis process were observed in the internal structure of the leaf. The wettability analysis showed that the abaxial side is more hydrophobic, while the adaxial side is more hydrophilic. AFM images exposed the relevant details of the microstructure of the leaf abaxial side, such as stomata, pores, furrows, contour, particles, and rough profiles generated by topographic irregularities. The statistical parameters revealed that the scale size influences the topographic roughness, surface asymmetry, and shape of the height distribution, also observed by advanced parameters obtained according to the standard of the international organization for standardization (ISO). The fractal and advanced fractal parameters confirmed changes in spatial patterns as a function of scale size. The largest area exhibited greater spatial complexity, low dominant spatial frequencies, more excellent surface percolation, intermediate topographic homogeneity, and high uniformity of spatial patterns.


2010 ◽  
Vol 73 (6) ◽  
pp. 1023-1029 ◽  
Author(s):  
MARILYN C. ERICKSON ◽  
CATHY C. WEBB ◽  
JUAN CARLOS DIAZ-PEREZ ◽  
SHARAD C. PHATAK ◽  
JOHN J. SILVOY ◽  
...  

Numerous field studies have revealed that irrigation water can contaminate the surface of plants; however, the occurrence of pathogen internalization is unclear. This study was conducted to determine the sites of Escherichia coli O157:H7 contamination and its survival when the bacteria were applied through spray irrigation water to either field-grown spinach or lettuce. To differentiate internalized and surface populations, leaves were treated with a surface disinfectant wash before the tissue was ground for analysis of E. coli O157:H7 by direct plate count or enrichment culture. Irrigation water containing E. coli O157:H7 at 102, 104, or 106 CFU/ml was applied to spinach 48 and 69 days after transplantation of seedlings into fields. E. coli O157:H7 was initially detected after application on the surface of plants dosed at 104 CFU/ml (4 of 20 samples) and both on the surface (17 of 20 samples) and internally (5 of 20 samples) of plants dosed at 106 CFU/ml. Seven days postspraying, all spinach leaves tested negative for surface or internal contamination. In a subsequent study, irrigation water containing E. coli O157:H7 at 108 CFU/ml was sprayed onto either the abaxial (lower) or adaxial (upper) side of leaves of field-grown lettuce under sunny or shaded conditions. E. coli O157:H7 was detectable on the leaf surface 27 days postspraying, but survival was higher on leaves sprayed on the abaxial side than on leaves sprayed on the adaxial side. Internalization of E. coli O157:H7 into lettuce leaves also occurred with greater persistence in leaves sprayed on the abaxial side (up to 14 days) than in leaves sprayed on the adaxial side (2 days).



2009 ◽  
Vol 72 (10) ◽  
pp. 2028-2037 ◽  
Author(s):  
GUODONG ZHANG ◽  
LI MA ◽  
LARRY R. BEUCHAT ◽  
MARILYN C. ERICKSON ◽  
VANESSA H. PHELAN ◽  
...  

Survival and internalization characteristics of Escherichia coli O157:H7 in iceberg, romaine, and leaf lettuce after inoculation of leaf surfaces and soil were determined. A five-strain mixture of E. coli O157:H7 in water and cow manure extract was used as an inoculum for abaxial and adaxial sides of leaves at populations of 6 to 7 log and 4 log CFU per plant. The five strains were individually inoculated into soil at populations of 3 and 6 log CFU/g. Soil, leaves, and roots were analyzed for the presence and population of E. coli O157:H7. Ten (4.7%) of 212 samples of leaves inoculated on the adaxial side were positive for E. coli O157:H7, whereas 38 (17.9%) of 212 samples inoculated on the abaxial side were positive. E. coli O157:H7 survived for at least 25 days on leaf surfaces, with survival greater on the abaxial side of the leaves than on the adaxial side. All 212 rhizosphere samples and 424 surface-sanitized leaf and root samples from plants with inoculated leaves were negative for E. coli O157:H7, regardless of plant age at the time of inoculation or the location on the leaf receiving the inoculum. The pathogen survived in soil for at least 60 days. Five hundred ninety-eight (99.7%) of 600 surface-sanitized leaf and root samples from plants grown in inoculated soil were negative for E. coli O157:H7. Internalization of E. coli O157:H7 in lettuce leaves and roots did not occur, regardless of the type of lettuce, age of plants, or strain of E. coli O157:H7.



Development ◽  
2002 ◽  
Vol 129 (18) ◽  
pp. 4281-4289
Author(s):  
Robert J. Meister ◽  
Louren M. Kotow ◽  
Charles S. Gasser

The outer integument of Arabidopsis ovules exhibits marked polarity in its development, growing extensively from the abaxial side, but only to a very limited extent from the adaxial side of the ovule. Mutations in two genes affect this asymmetric growth. In strong inner no outer (ino) mutants outer integument growth is eliminated, whereas in superman (sup) mutants integument growth on the adaxial side is nearly equal to wild-type growth on the abaxial side. Through complementation and reporter gene analysis, a region of INO 5′-flanking sequences was identified that contains sufficient information for appropriate expression of INO. Using this INO promoter (P-INO) we show that INO acts as a positive regulator of transcription from P-INO, but is not sufficient for de novo initiation of transcription in other plant parts. Protein fusions demonstrate nuclear localization of INO, consistent with a proposed role as a transcription factor for this member of the YABBY protein family. Through its ability to inhibit expression of the endogenous INO gene and transgenes driven by P-INO, SUP is shown to be a negative regulator of INO transcription. Substitution of another YABBY protein coding region (CRABS CLAW) for INO overcomes this negative regulation, indicating that SUP suppresses INO transcription through attenuation of the INO positive autoregulatory loop.



Phytotaxa ◽  
2019 ◽  
Vol 391 (3) ◽  
pp. 167 ◽  
Author(s):  
MIN-JUNG KONG ◽  
SUK-PYO HONG

A comparative study of the leaf microstructures of 19 taxa belonging to the Persicaria sect. Cephalophilon and related four Koenigia taxa was performed by LM and SEM to evaluate their systematic significance. Both amphistomatic and hypostomatic leaves were observed in the taxa studied. The stomatal size ranged from 17.04–41.96 × 13.41–37.30 μm, and stomata on the adaxial side were larger than those on the abaxial side in general. Anomocytic stomata occurred most commonly, but more than one type of stomata was observed on the same surface. Paracytic stomata was found in both Persicaria palmata and P. criopolitana. The epidermal cells usually have straight to sinuate anticlinal cell walls (ACW), and the ACW on the abaxial side of most taxa is much more undulated than that on the adaxial side. Cuticular striation was observed in most of the studied taxa, which was restricted to only the adaxial side. Two types of crystals were observed: druse and prismatic, and seven types of trichome were recognized: five types of non-glandular trichomes (stellate with smooth surface, multiseriate with either smooth or striated surface, and uniseriate with either smooth or papillose surface), and two types of glandular trichomes (peltate and long-stalked pilate). The leaf micromorphology in this study was categorized into five types based on the stomata, epidermis, crystal and trichome, as further systematic significance of the leaf epidermal characters within the P. sect. Cephalophilon are discussed. In addition, we propose a new taxonomic combination in the P. sect. Cephalophilon.



2014 ◽  
Vol 675-677 ◽  
pp. 1120-1124
Author(s):  
Zi Song Yang

In this paper, the correlation dimension of the population distribution of L. regale were analyzed by fractal theory. The results show: (1) The fractal characters in different areas are obvious; (2)In most cases, the correlation dimension of L. regale population are so high ranged from 1.4664 to 1.7384, indicating higher individual spatial correlation degree and little difference of the scaling properties of spatial autocorrelation of individuals in different plots; (3)Irregular distributions, and great difference of scaling; (4) Ten correlation dimensions of L. regale are changing as the latitude regularly decreases or increases.



2018 ◽  
Vol 10 (4) ◽  
pp. 547-553
Author(s):  
Bhavna Girish SHARMA ◽  
Susy ALBERT ◽  
Haresh Kumar DHADUK

Leaves of thirty cultivars of Mangifera indica L. were investigated to compare their anatomical variations and identify the characteristic features which are potential markers for the identification of the cultivars. Variations were noted in the thickness of cuticle, length of epidermal cells in the abaxial and adaxial surfaces, length of palisade and spongy tissue. The length of epidermal cell varied from 10 µm in ‘Goto’ to 25 µm in ‘Desi’ cultivars on adaxial side, while on the abaxial side it varied from 15.5 µm in ‘Alphonso’ to 6.9 µm in ‘Sopari’. The palisade tissue length was maximum in ‘Jahangir’ (111.36 µm), while it was lowest in ‘Fazli’ (24.13 µm). Spongy tissue length was the highest in ‘Jamadar’ (199.92 µm) and lowest in ‘Fazli’ (90.55 µm). Two layers of palisade tissue were seen in ‘Sindoria’, ‘Jhumakhiya 2’, ‘Aambadi’, ‘Neelam’,  ‘Rajapuri’, ‘Fazli’, ‘Jahangir’, ‘Kaju’, and ‘Aamir pasand’, while three layers were seen in ‘Alphonso’, ‘Jamadar’, ‘Ladvo’, ‘Sopari’ and ‘Dudhpendo’. Such parameters can be used for distinctly differentiating varieties among them and thus have an exact identification when morphological features are indistinguishable.



1987 ◽  
Vol 65 (9) ◽  
pp. 1870-1875 ◽  
Author(s):  
L. Citharel ◽  
J. Citharel

The presence of globoids in cotyledon protein bodies is confirmed in several Genistae. In addition, the duality (homogeneous protein bodies in cells on the adaxial side and heterogeneous protein bodies in cells on the abaxial side), previously reported in Cytisus scoparius cotyledons, has now been established in other Cytisus species, as well as in other genera of the Genistae tribe.



Geophysics ◽  
2010 ◽  
Vol 75 (3) ◽  
pp. R47-R59 ◽  
Author(s):  
R. P. Srivastava ◽  
M. K. Sen

In general, inversion algorithms rely on good starting models to produce realistic earth models. A new method, based on a fractional Gaussian distribution derived from the statistical parameters of available well logs to generate realistic initial models, uses fractal theory to generate these models. When such fractal-based initial models estimate P- and S-impedance profiles in a prestack stochastic inversion of seismic angle gathers, very fast simulated annealing — a global optimization method — finds the minimum of an objective function that minimizes data misfit and honors the statistics derived from well logs. The new stochastic inversion method addresses frequencies missing because of band limitation of the wavelet; it combines the low- and high-frequency variation from well logs with seismic data. This method has been implemented successfully using real prestack seismic data, and results have been compared with deterministic inversion. Models derived by a deterministic inversion are devoid of high-frequency variations in the well log; however, models derived by stochastic inversion reveal high-frequency variations that are consistent with seismic and well-log data.



Author(s):  
Jiang Zhao ◽  
zekun wang ◽  
Zhengminqing Li ◽  
Rupeng Zhu

Abstract A machined surface has observable fractal characteristics, with infinite local and overall self-similar consistency. Therefore, the fractal theory is considered to provide a better description of the morphological characteristics of rough surfaces, which accurately reflects the randomness and multi-scale characteristics of rough surfaces and it is not comparable with the surface characteristics obtained based on statistical parameters limited by sampling length and device resolution. In this study, the Weierstrass-Mandelbrot (W-M) function was applied to construct a fractal reconstruction surface, and the mixed elastohydrodynamic lubrication model was used to investigate the lubrication characteristics of real and reconstructed surfaces under the same fractal parameters. The effects of the fractal parameters on the fractal surface lubrication characteristics were further analyzed. The results demonstrate that the lateral roughness fractal surface provides greater resistance to the entrained flow of lubricant, which leads to a larger average film thickness, than the longitudinal roughness and isotropic fractal surface. With the increase in fractal dimension, the surface roughness peak density increases, which reduces the surface film thickness by 47%, and the friction coefficient increases by 46%. The lubrication parameter fluctuates slightly with the change in the number of overlapping ridges M of the fractal surface. Generally, M has little effect on the surface lubrication characteristics.



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