scholarly journals Probiotic Characteristics of Ligilactobacillus salivarius AS22 Isolated from Sheep Dung and Its Application in Corn-Fox Tail Millet Silage

2021 ◽  
Vol 11 (20) ◽  
pp. 9447
Author(s):  
Balamuralikrishnan Balasubramanian ◽  
Ilavenil Soundharrajan ◽  
Naif Abdullah Al-Dhabi ◽  
Ponnuswamy Vijayaraghavan ◽  
Kaleeswaran Balasubramanian ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Animal Feed ◽  
Ph Value ◽  
Lactic Acid Production ◽  
Foxtail Millet ◽  
High Growth ◽  
Antimicrobial Activities ◽  
Matter Content ◽  
Dry Matter Content ◽  
Forage Crops

The forage crops corn (Zea mays) and foxtail millet (Panicum italicum L.) are widely used as animal feed because of their high nutritive values. The ensiling of corn and foxtail millet is often associated with the growth of undesirable microbes, which cause severe loss of dry matter content during the storage periods. The selection of suitable Ligilactobacillus species for corn-fox tail millet silage production can improve the quality. In this study, we aimed to select potent lactic acid bacteria (LAB) from sheep dung and analyses their biological application such as probiotic features, antimicrobial activities and fermentation capability of silage. A total of nine Lactobacillus strains were inoculated in MRS medium to evaluate lactic acid concentration. The isolated strain, Ligilactobacillus salivarius AS22, produced a higher lactic acid level (40.2 ± 2.2 µg/mL) with high growth rates (2.24 ± 0.12 OD at 600 nm) compared to other strains. The silage treated with inoculant (L. salivarius AS22) decreased the pH value (p < 0.05) and enhanced lactic acid production (p < 0.05) than the control at ensiled silages. LAB inoculated silage had reduced numbers of fungal colonies than control (p < 0.05). In conclusion, the addition of L. salivarius AS22 improved the quality of whole corn and foxtail millet silages with significant probiotic potential.

scholarly journals Effect of the different level of a dry feed additive on the lupin silage quality

2005 ◽  
Vol 53 (5) ◽  
pp. 21-30
Author(s):  
Petr Doležal ◽  
Josef Rotter ◽  
Jan Doležal ◽  
Václav Pyrochta ◽  
Jaroslav Poul
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Ph Value ◽  
Total Content ◽  
Matter Content ◽  
Feed Additive ◽  
Dry Matter Content ◽  
Higher Alcohol

In the experiment, the effect of additive on the fermentation quality of lupine was examined, by comparing with the untreated control. Fresh green Lupine (Lupines lupine), variete Juno, dry matter content 187.15 g/kg at full waxy stage of maturiy were chopped to the legth of cut ca 30–50 mm. The crop was artificially wilted for a periody 24 h and ensiled as described above. Lupine were ensiled for 98 days in laboratory silos, capacity about 4 L alone or with supplementation of feed additive (5, 10, 20, 30, 40, 50 or 70 kg/tone forage respectively). The composition and as effective substances of this silage ingredient were dry whey (30%), maize meal (40%) and dry molases (30%). The silages fermented rapidly and changes in volatile fatty acids (VFA) production (P<0.01) and in sum of acids were noted. The different supplementation of additive in our experiment conditions increased significantly (P<0.01) the dry matter content, and decreased statistically significantly (P<0.01) the pH value. The higher DM content was in all experimental silages, but the highest DM content (P<0.01) was in silage with addition of 70 kg/t (231.58Ī0.91 g/kg). All treated silages were well fermented with low levels of ammonia and pH. The different addition of this aditive increased significantly (P<0.01) the contents of lactic acid and total content of acids in comparison with control silage. The experimental silage with higher (5%, resp. 7%) feed supplementation was of better quality (significantly higher ratio LA/sum of acids, higher content of lactic acid, lower NH3 content and pH value) than the control silage, or silage with lower concentration. In experiment feed additive–treated silages (by groups of 5 or 50 and 70 kg/t) had significantly higher alcohol content than untreated silage. It was concluded that feed additive used as a silage additive improved fermentation of lupine, reduced acetic acid and ammonia production and increased silage nutritive value.

scholarly journals Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs

2021 ◽  
Vol 9 (7) ◽  
pp. 1346
Author(s):  
Mariana Petkova ◽  
Petya Stefanova ◽  
Velitchka Gotcheva ◽  
Angel Angelov
Keyword(s):  
Lactic Acid ◽  
Sequence Analysis ◽  
Lactic Acid Bacteria ◽  
Antimicrobial Properties ◽  
Matter Content ◽  
Starter Cultures ◽  
Rrna Gene ◽  
Dry Matter Content ◽  
Isolation And Characterization ◽  
Selection Of

Traditional sourdoughs in Bulgaria were almost extinct during the centralized food production system. However, a rapidly developing trend of sourdough revival in the country is setting the demand for increased production and use of commercial starter cultures. The selection of strains for such cultures is based on geographical specificity and beneficial technological properties. In this connection, the aim of this study was to isolate, identify and characterize lactic acid bacteria (LAB) and yeasts from typical Bulgarian sourdoughs for the selection of strains for commercial sourdough starter cultures. Twelve samples of typical Bulgarian sourdoughs were collected from different geographical locations. All samples were analyzed for pH, total titratable acidity and dry matter content. Enumeration of LAB and yeast was also carried out. Molecular identification by 16S rDNA sequence analysis was performed for 167 LAB isolates, and 106 yeast strains were identified by ITS1-5.8S-ITS2 rRNA gene partial sequence analysis. The LAB strains were characterized according to their amylolytic and proteolytic activity and acidification capacity, and 11 strains were selected for further testing of their antimicrobial properties. The strains with the most pronounced antibacterial and antifungal activity are listed as recommended candidates for the development of starter cultures for sourdoughs or other food products.

Carbohydrate Metabolism of Chick Fibroblasts in Vitro

1942 ◽  
Vol 18 (3) ◽  
pp. 237-256
Author(s):  
E. N. WILLMER
Keyword(s):  
Lactic Acid ◽  
Carbohydrate Metabolism ◽  
Acid Production ◽  
Lactic Acid Production ◽  
High Growth ◽  
Glucose Consumption ◽  
High Growth Rate ◽  
Absolute Correlation ◽  
Heart Cultures

1. The glucose consumption of osteoblasts growing in a medium of plasma and embryo juice has been compared with that of the same cells in plasma and Tyrode medium. Embryo juice causes an initial increased glucose consumption which later falls below the level characteristic of a Tyrode-plasma medium. 2. A modification of a method of measuring the nucleoprotein phosphorus content of tissue cultures is described and among other data obtained the changes which occur in this figure have been noted for fourth passage chick osteoblast cultures and for fresh heart explants during the first 4 days of culture in media of plasma and Tyrode and of plasma and embryo juice. 3. The figures for nucleoprotein content have been correlated with those for glucose consumption and lactic acid production and pronounced differences have been observed in the behaviour of the two types of tissue. The possible significance of the findings is discussed. 4. There is no absolute correlation between high growth rate and high sugar consumption or lactic acid production. 5. The data discussed are consistent with the idea that an increased protein metabolism, and a decreased carbohydrate metabolism result from the addition of embryo juice to osteoblast cultures. Embryo juice has no detectable effects on the carbohydrate metabolism of fresh heart cultures.

scholarly journals Effects of chemical preservative and pressing of ensiled sugar-beet pulp on the quality of fermentation process

2011 ◽  
Vol 50 (No. 12) ◽  
pp. 553-560 ◽  
Author(s):  
P. Doležal ◽  
V. Pyrochta ◽  
J. Doležal
Keyword(s):  
Lactic Acid ◽  
Sugar Beet ◽  
Dry Matter ◽  
Fermentation Process ◽  
Ph Value ◽  
Sugar Beet Pulp ◽  
Dry Matter Content ◽  
Chemical Preservative ◽  
Beet Pulp

This study deals with effects of pressing of ensiled sugar-beet pulp and of application of a chemical preservative on the quality of fermentation process. The experimental silages had a better sensory evaluation than the control ones. In silages treated chemically with a mixture of acids, statistically significantly (P &lt; 0.01) higher dry matter content, lowest pH value, the value of lactic acid and the lowest content of all acids in dry matter were found after 180 days of storage from the beginning of the experiment. The statistically significantly (P &lt; 0.01) highest lactic acid content (43.39 &plusmn; 1.25 g/kg DM) was determined in the control pressed silage. The highest LA/VFA ratio (1.40 &plusmn; 0.18) was calculated for non-pressed experimental silage (D &ndash; 3 l/t of KEM). As compared with untreated control the highest percentage (P &lt; 0.01) of lactic acid and of all fermentation acids was found out in silage D treated with 3 l/t of KEM (58.18 &plusmn; 0.47 g/kg DM). Undesirable butyric and propionic acids were not found in chemically treated silage samples (C, D, E, F). However, the highest (P &lt; 0.01) contents of butyric acid (26.37 &plusmn; 0.91 g/DM) and propionic acid (4.58 &plusmn; 0.78 g/DM) were measured in untreated non-pressed silage samples (B). The highest (P &lt; 0.01) contents of acetic acid and ethanol were found in control silage samples. The quality of these silages was evaluated as very low. &nbsp;

Effect of cereal and pea monocultures and combinations and silage additives on whole-crop cereal silage nutritive and fermentation characteristics

2013 ◽  
Vol 53 (5) ◽  
pp. 427 ◽  
Author(s):  
J. L. Jacobs ◽  
G. N. Ward
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Nutritive Value ◽  
Matter Content ◽  
Efficient Production ◽  
Dry Matter Content ◽  
Total N ◽  
Winter Cereals ◽  
Fermentation Parameters ◽  
Silage Additives

The efficient production and utilisation of home-grown feed is considered one of the key factors that underpins the profitability of dairy systems in southern Australia. The use of winter forage cereals for grazing and silage provides an opportunity to achieve high dry matter yields over the winter and spring period. However, questions remain on the nutritive value of whole-crop cereal silage and its subsequent use as a production feed in livestock systems. This experiment examined the nutritive characteristics of winter wheat, triticale, forage peas and bi-crops of cereals and peas sown at different proportions, cut for silage at the soft dough growth stage of the cereals and their subsequent silage nutritive characteristics and fermentation patterns when ensiled with and without bacterial inoculant additives over 2 consecutive years. The estimated metabolisable energy (ME) (Year 1) and crude protein (CP) (Years 1 and 2) concentrations of the forage pea before and after ensiling were higher (P < 0.05) than all other forages in both years. The cereal–pea mixes had similar estimated ME values to the cereal monocultures both before harvesting and as silage, although there were significant improvements in CP concentration at the higher rates of pea inclusion. All resultant silages were well fermented as indicated by low pH, low proportions of total N as ammonia-N and high lactic acid concentrations. There were marked differences in the proportions of lactic acid and acetic acid in the pea silages between years and this is likely a result of dry matter content differences at ensiling. There was no effect of silage additives on resultant silage nutritive characteristics or fermentation parameters indicating that well fermented silage can be achieved without the additional cost of using a silage additive. This study has indicated that forage peas can be ensiled with winter cereals and produce silages that have higher CP concentrations than cereal silage but with similar fermentation parameters. Furthermore, this experiment has highlighted the potential of growing a monoculture of forage peas for ensiling with the resulting silage having higher estimated ME and CP concentrations.

Influence of wilting time on silage compositional quality and microbiology

2001 ◽  
Vol 2001 ◽  
pp. 88-88 ◽  
Author(s):  
I.A. Bouriako ◽  
H. Shihab ◽  
V. Kuri ◽  
J. K. Margerison
Keyword(s):  
Dry Matter ◽  
Microbial Growth ◽  
Matter Content ◽  
Dry Matter Content ◽  
Forage Crops ◽  
Fermentation Quality ◽  
Feeding Value ◽  
Silage Quality ◽  
Compositional Quality

Wilting grass before ensiling has become a firmly established practice, since it provides economic advantages due to the increment of the dry matter content of the forage before ensiling. Most forage crops contain less than 20 per cent of dry matter when they are cut thus reduction of the weight of crop to be transported provides advantages (Gordon et al., 1999). Also, research has shown that such practices yield improved and reduced effluent loss from the silo and higher feeding value of the silage. Wilting of grass prior to ensiling has been widely adopted as a means of reducing effluents produced and improving the fermentation quality of silage. The aim of this research was to assess the effect of differing wilting periods and silage moisture levels of silage quality and microbial growth levels.

scholarly journals Lactic acid production from cane molasses

2017 ◽  
Vol 2 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Aladár Vidra ◽  
András József Tóth ◽  
Áron Németh
Keyword(s):  
Heavy Metals ◽  
Lactic Acid ◽  
Acid Production ◽  
Animal Feed ◽  
Sugar Content ◽  
Lactic Acid Production ◽  
Raw Material ◽  
Lactobacillus Species ◽  
Alcohol Production ◽  
Study Results

Abstract Molasses, a by-product of the sugar manufacturing process, generally comprises approximately 50% (w/w) of total sugars, but it is currently used primarily [1] as an animal feed and as a raw material in alcohol production. Currently, the sugar production is more than 160 million tones worldwide. Its byproduct molasses contain heavy metals which have growthinhibitory effect. The main sugar content in molasses is sucrose which often need to be hydrolyzed to glucose and fructose especially for utilization by Lactobacillus species. Lactobacillus species can convert sugar content to lactic acid with great efficiency, which is a valuable chemical. Lactic acid production from sugar molasses using batch fermentations with Lactobacillus casei and Lactobacillus sp. MKT878 were investigated in this study. Results showed, that both examined Lactobacillus species could grow on molasses despite the heavy metals inhibitory effects. The conversion of sugar content to lactic acid was successful with yield between 55-80 g/g.

scholarly journals KUALITAS SILASE DAUN GAMAL DENGAN PENAMBAHAN MOLASES SEBAGAI ZAT ADITIF

2017 ◽  
Vol 7 (2) ◽  
Author(s):  
Ervi Herawati ◽  
Mega Royani
Keyword(s):  
Lactic Acid ◽  
Animal Feed ◽  
Ph Value ◽  
Gliricidia Sepium ◽  
Feed Material ◽  
Randomized Design ◽  
Coumarin Compound ◽  
Optimal Quality ◽  
Acid Percentage

AbstrakGamal adalah tanaman leguminosa pohon yang dapat tumbuh dengan cepat namun pemanfaatannya sebagai bahan pakan ternak memiliki palatabilitas yang rendah akibat bau dan rasanya yang menyengat dan pahit, berasal dari senyawa kumarin, sehingga kurang disukai oleh ternak. Salah satu alternatif yang dapat digunakan untuk menghilangkan zat antinutrisi tersebut yaitu dengan cara dibuat silase. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan molasses terhadap kualitas silase pada daun gamal terfermentasi yang memberikan hasil silase terbaik. Uji kualitas yang diukur adalah pH, persentase asam laktat, dan kadar amonia. Metode penelitian yang digunakan adalah metode eksperimental dengan metode Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 4 ulangan. Perlakuannya terdiri dari penambahan molasses pada daun gamal yaitu P1=1%, P2=2%, P3=3%, P4=4%, dan P5=5%. Hasil pengujian menunjukkan bahwa kualitas silase daun gamal yang optimal didapat pada perlakuan 4 (P4=4% molasses) yang menghasilkan rata-rata nilai pH sebesar 4,18, persentase asam laktat 1,143%, dan kadar NH3 sebesar 6,06 mM. Kata kunci: asam laktat, gamal, molases, NH3, pH. AbstractGliricidia sepium is a leguminous tree that grows rapidly but its use as animal feed material has low palatability due to its stinky smell and bitter taste, caused by coumarin compound, hence it is less favored by livestock. One alternative way to remove this compound is by making silage. This study was aimed to determine the effect of adding molasses to the quality of silage on fermented Gliricidia sepium leaves to obtain the best silage result. Quality tests measured include pH, lactic acid percentage, and ammonia levels. Method used was experimental Completely Randomized Design with 5 treatments and 4 replications. The treatment consisted of the addition of molasses on the Gliricidia sepium leaf, P1 = 1%, P2 = 2%, P3 = 3%, P4 = 4%, and P5 = 5%. Results showed that the optimal quality of Gliricidia sepium leaf silage was obtained from P4 (4% molasses) which resulted in average pH value of 4.18, lactic acid percentage 1.143%, and NH3 level of 6.06 mM.Keywords: lactic acid, Gliricidia sepium, molasses, NH3, pH.

scholarly journals The quality of silage of corn grain and spent P. ostreatus mushroom substrate

2007 ◽  
pp. 211-218 ◽  
Author(s):  
Milan Adamovic ◽  
Aleksandra Bocarov-Stancic ◽  
Ivanka Milenkovic ◽  
Snezana Strbac ◽  
Ivana Adamovic
Keyword(s):  
Volatile Fatty Acids ◽  
Ph Value ◽  
Matter Content ◽  
Dry Matter Content ◽  
Wood Shaving ◽  
Fermentation Quality ◽  
Substrate Ratio ◽  
Ground Corn ◽  
Corn Grain

The chemical composition, fermentation quality, mycological and mycotoxicological analyses of silage mixture, made of ground corn grain and spent P. ostreatus mushroom substrate, were investigated in this paper. Dry matter content in high moisture ground corn, at the time of ensiling was 70%, and in the spent substrate (on the Salt Cedar wood shaving basis) was 52.7%. Corn grain to spent substrate ratio in trials was: 100:0% (I), 90:10% (II), 80:20% (III) and 70:30% (IV) respectively. Content of the lignocellulose fractions in silage was slightly increased, and protein content was slightly decreased with the increase of spent substrate content. Contents of the VFA (volatile fatty acids) in silage, pH value, and NH3-N content were for the silage of very good quality. In the spent substrate 9 mold species were found, from which the most frequent were genus Penicillium, Paecilomyces variotii, and Trichoderma harzianum. In ground corn grain silage (I) presence of the yeasts was dominant (90.000/g). In combined trials (II-IV) only Penicillium (P. brevicompactum and P. echinulatum) mold species were found. Presence of molds and yeasts in investigated trials was within tolerated values for ensiled feedstuffs. Mycotoxin presence in silage was not determined.

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