scholarly journals Synchrotron FTIR Microspectroscopy Investigations on Biochemical Changes Occurring in Human Cells Exposed to Proton Beams

2021 ◽  
Vol 12 (1) ◽  
pp. 336
Author(s):  
Ines Delfino ◽  
Valerio Ricciardi ◽  
Maria Lepore

Fourier transform infrared microspectroscopy using a synchrotron radiation source (SR-μFTIR) has great potential in the study of the ionizing radiation effects of human cells by analyzing the biochemical changes occurring in cell components. SR-μFTIR spectroscopy has been usefully employed in recent years in some seminal work devoted to shedding light on processes occurring in cells treated by hadron therapy, that is, radiotherapy with charged heavy particles (mainly protons and carbon ions), which is gaining popularity as a cancer treatment modality. These studies are particularly useful for increasing the effectiveness of radiotherapy cancer treatments with charged particles that can offer significant progress in the treatment of deep-seated and/or radioresistant tumors. In this paper, we present a concise revision of these studies together with the basic principles of μFTIR spectroscopy and a brief presentation of the main characteristics of infrared SR sources. From the analysis of the literature regarding the SR-μFTIR spectroscopy investigation on human cells exposed to proton beams, it is clearly shown that changes in DNA, protein, and lipid cell components are evident. In addition, this review points out that the potential offered by SR-μFTIR in investigating the effects induced by charged particle irradiation have not been completely explored. This is a crucial point for the continued improvement of hadron therapy strategies.

2019 ◽  
Vol 186 (2-3) ◽  
pp. 172-175 ◽  
Author(s):  
Werner Friedland ◽  
Pavel Kundrát ◽  
Janine Becker ◽  
Markus Eidemüller

ABSTRACT The biophysical simulation tool PARTRAC has been primarily developed to model radiation physics, chemistry and biology on nanometre to micrometre scales. However, the tool can be applied in simulating radiation effects in an event-by-event manner over macroscopic volumes as well. Benchmark simulations are reported showing that PARTRAC does reproduce the macroscopic Bragg peaks of proton beams, although the penetration depths are underestimated by a few per cent for high-energy beams. PARTRAC also quantifies the increase in DNA damage and its complexity along the beam penetration depth. Enhanced biological effectiveness is predicted in particular within distal Bragg peak parts of therapeutic proton beams.


2001 ◽  
Vol 692 ◽  
Author(s):  
R. J. Walters ◽  
G. P. Summers

AbstractAn investigation of the physical mechanisms governing the response of III-V based solar cells to particle irradiation is presented. The effect of particle irradiation on single and multijunction solar cells is studied through current vs. voltage, spectral response, and deep level transient spectroscopy measurements. The basic radiation response mechanisms are identified, and their effects on the solar cell electrical performance are described. In particular, a detailed analysis of multijunction InxGa1-xP/InyGa1-yAs/Ge devices is presented. The MJ cell response is found to be more strongly affected by the internal cell structure than by the In content.


Author(s):  
Margaret A. McMahan ◽  
Ewart Blackmore ◽  
Ethan W. Cascio ◽  
Carlos Castaneda ◽  
Barbara von Przewoski ◽  
...  

Author(s):  
Y. Ito ◽  
K. Yasuda ◽  
M. Sasase ◽  
R. Ishigami ◽  
S. Hatori ◽  
...  

2001 ◽  
Vol 20 (4) ◽  
pp. 325-338 ◽  
Author(s):  
Sejuty HAQUE ◽  
Shuichi TAKINAMI ◽  
Fumio WATARI ◽  
Mahfujul Haq KHAN ◽  
Motoyasu NAKAMURA

Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2418 ◽  
Author(s):  
Maria Lasalvia ◽  
Giuseppe Perna ◽  
Vito Capozzi

The toxicological implications of nanoparticles deserve accurate scientific investigation for the protection of human health. Although toxic effects involve specific organs, the events that cause them have their origin from biochemical modifications of some cellular constituents. Therefore, a first analysis to evaluate the effects due to the action of nanoparticles is achieved by investigation of in vitro cells, which allows the identification of the cellular modifications caused by nanoparticles (NPs) even at much lower doses than the lethal ones. This work evaluated the Raman microspectroscopy capability to monitor biochemical changes occurring in human cells as a consequence of exposure to a suspension of gold nanoparticles with a non-cytotoxic concentration. Human keratinocyte cells were used as a model cell line, because they are mainly involved in environmental exposure. A trypan blue assay revealed that the investigated concentration, 650 ng/mL, is non-cytotoxic (about 5% of cells died after 48 h exposure). Specific Raman spectral markers to represent the cell response to nanoparticle exposure were found (at 1450 and 2865 cm−1) in the cytoplasm spectra, with the aid of ratiometric and principal component analysis.


2014 ◽  
Vol 89 (5) ◽  
Author(s):  
J. Dudouet ◽  
D. Cussol ◽  
D. Durand ◽  
M. Labalme

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