Biocompatibility and Antioxidant Capabilities of Carbon Dots Obtained from Tomato (Solanum lycopersicum)

Since their discovery in 2004, carbon dots have attracted strong interest in the scientific community due to their characteristic properties, particularly their luminescence and their ease of synthesis and derivatization. Carbon dots can be obtained from different carbon sources, including natural products, resulting in a so-called ’green synthesis’. In this work, we obtain carbon dots from tomato juice in order to obtain nanoparticles with the antioxidant capabilities of the natural antioxidants present in that fruit. The obtained material is characterized regarding nanoparticle size distribution, morphology, surface functional groups and optic properties. Antioxidant properties are also evaluated through the DPPH method and their cytotoxicity is checked against human dermal fibroblast and A549 cell-lines. The results indicate that carbon dots obtained from tomato have a higher antioxidant power than other already-published antioxidant carbon dots. The bandgap of the synthesized materials was also estimated and coherent with the literature values. Moreover, carbon dots obtained from tomato juice are barely toxic for healthy cells up to 72 h, while they induce a certain cytotoxicity in A549 lung carcinoma cells.

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Antioxidant Capacity and Phenolic and Sugar Profiles of Date Fruits Extracts from Six Different Algerian Cultivars as Influenced by Ripening Stages and Extraction Systems

Foods ◽

10.3390/foods10030503 ◽

2021 ◽

Vol 10 (3) ◽

pp. 503

Author(s):

Malika Tassoult ◽

Djamel Edine Kati ◽

María África Fernández-Prior ◽

Alejandra Bermúdez-Oria ◽

Juan Fernandez-Bolanos ◽

...

Keyword(s):

Sugar Content ◽

Antioxidant Properties ◽

Dry Weight ◽

Natural Antioxidants ◽

Antioxidant Power ◽

Fruit Extracts ◽

Ripening Stages ◽

Date Fruit ◽

Ferric Reducing Antioxidant Power ◽

First Time

The study investigated the phenols, sugar and the antioxidant capacities of date fruit extracts obtained by organic solvents and by hydrothermal treatment from six different Algerian cultivars at two ripening stages for the first time. The analyzed cultivars exhibited potent antioxidant properties (ferric reducing antioxidant power (FRAP), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging capacities) and different phenols regardless of the solvents and the maturity stages. About 18 phenols were identified and quantified, mainly in the hydrothermal extracts. The earlier stages were characterized by high amounts of o-coumaric acid, cinnamic acid and luteolin, with a noticeable absence of quercetin. The tamr stage presented the highest sugar content (78.15–86.85 mg/100 mg dry weight (DW)) with an abundance of glucose. Galactose was present only in some cultivars from the kimri stage (tamjouhert). Uronic acids were mostly detected at the tamr stage (4.02–8.82 mg gallic acid equivalent/100 mg dried weight). The obtained results highlight the potential of using date fruit extracts as natural antioxidants, especially at industrial scales that tend use hydrothermal extraction.

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POTENSI SENYAWA BIOAKTIF MESOCARP BUAH LONTAR (Borassus ﬂ abeliffer L.) SEBAGAI SUMBER ANTIOKSIDAN ALAMI (Potency of Mesocarp Bioactive Compounds in Lontar Fruit (Borassus ﬂ abeliffer L.) as A Source of Natural Antioxidant)

Jurnal Agritech ◽

10.22146/agritech.9455 ◽

2014 ◽

Vol 34 (03) ◽

pp. 277 ◽

Cited By ~ 1

Author(s):

Eny Idayati ◽

Suparmo Suparmo ◽

Purnama Darmadji

Keyword(s):

Bioactive Compounds ◽

Antioxidant Properties ◽

Chemical Properties ◽

Natural Antioxidants ◽

Chromatography Method ◽

Carotene Content ◽

Palm Fruit ◽

Dpph Method ◽

Total Fat ◽

Physical And Chemical

This study was aimed to characterize the physical and chemical properties of borassus palm fruit mesocarp, to determine the best type of solvent to extract bioactive compounds by maceration method, and to evaluate the antioxidant properties of bioactive compounds using DPPH (1,1-difenil-2-pikrilhidrazil) method. Carotenoid was separated based on TLC(thin layer chromatography) method, which produced spots. To conﬁ rm the results, the spots were scanned using UV-vis spectrofotometry. The results showed that the water content of borassus fruit mesocarp was 77.31%, while total fat, ash content, and tannin were 0.11%, 1.43%, 0.08%, respectively. Total Carotenoid was 8324.6 µg/100g with β carotene content was 6217.48 microgram/100g. The best solvent used in extracting the bioactive compounds was ethanol and acetone with (1:1) ratio. The highest yield was 4.3% and bioactive compounds in palm fruit mesocarpextracts as antioxidants was about 87% as carried out by DPPH method, so it could become a potential antioxidant. TLC Identiﬁ cation produced two spots. One spot was identiﬁ ed as carotenoids from xanthophyll group and the other one was β-carotene.Keywords: Natural antioxidants, borassus palm fruit mesocarp, bioactive compounds ABSTRAKPenelitian ini bertujuan untuk karakterisasi senyawa bioaktif dalam mesocarp buah lontar berdasarkan sifat ﬁsik dan kimia, mengetahui jenis pelarut terbaik untuk mengekstrak senyawa bioaktif dengan metode maserasi, serta mengevaluasi sifat antioksidan senyawa bioaktif mesocarp dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Dasarpemisahan karotenoid dengan metoda KLT (kromatograﬁ lapis tipis) pada ekstrak lalu dikuatkan dengan hasil scanning menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa mesocarp buah lontar mengandung kadar air 77,31%; total lemak 0,11%, kadar abu 1,43%; tanin 0,08%; total karotenoid 8324,6 µg/100g dengan kandungan senyawa karoten 6217,48 µg/100g. Perlakuan pelarut terbaik untuk proses ekstraksi senyawa bioaktif adalah etanol dan aseton dengan rasio (1:1). Hasil rendemen tertinggi yaitu 4,3% dan potensi senyawa bioaktif dalam ekstrak mesocarp buah lontar sebagai antioksidan dengan metode DPPH yaitu sekitar 87%, sehingga berpotensi sebagai salahsatu senyawa antioksidan. Identiﬁ kasi dengan metode KLT yang menghasilkan 2 noda yaitu noda 1 diduga karotenoid dari golongan xantoﬁ l dan noda 2 yatu karoten.Kata kunci: Antioksidan alami, mesocarp lontar, senyawa bioaktif

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The Effect of Parietin Isolated From Rheum ribes L on In Vitro Wound Model Using Human Dermal Fibroblast Cells

The International Journal of Lower Extremity Wounds ◽

10.1177/1534734618819660 ◽

2019 ◽

Vol 18 (1) ◽

pp. 56-64 ◽

Cited By ~ 1

Author(s):

Gulsah Gundogdu ◽

Koksal Gundogdu ◽

Kemal Alp Nalci ◽

Alper Kursat Demirkaya ◽

Seymanur Yılmaz Tascı ◽

...

Keyword(s):

Cell Proliferation ◽

Cell Viability ◽

Dermal Fibroblast ◽

Dose Range ◽

Human Dermal Fibroblast ◽

Fibroblast Cells ◽

Wound Model ◽

Dpph Method ◽

Pipette Tip

Parietin is one of the well-known anthraquinone compounds that can be extracted from Rheum ribes L. In this study, we aimed to investigate the effects of parietin isolated from Rheum ribes L on an in vitro wound model using human dermal fibroblast cells and compare its effectiveness against zinc. The antioxidant effect of parietin was determined by using the 1,1-diphenyl-2-picrylhydrazine (DPPH) method. Human dermal fibroblast cells were cultured in proculture medium and were kept until 100% confluence was achieved. The wound model was created by using a pipette tip. After that, different concentrations of parietin and zinc (final concentrations in the well to be 5-250 µM and 25-200 µM, respectively) were added into the medium. The proliferation-inducing effect on cell viability was determined by using MTT assay. Images of cells were taken at 0, 12, and 24 hours. According to the DPPH method, parietin exhibited have antioxidant activity. According to the MTT results, parietin exhibited significant proliferation-inducing effect on cell viability in a dose range of 5 to 10 M, and zinc showed significant proliferation-inducing effect on cell viability at dose 50 µM ( P < .05). In addition, the image of cell proliferation was also shown at the same doses at 24 hours. In this study, we claim that parietin induces cell proliferation at low doses in cases of dermal fibroblast loss. In conclusion, parietin as an alternative to zinc in wound healing could be used by clinicians in the future with more extensive studies.

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Antioxidant potential of Pfaffia glomerata and Arrabidaea chica extracts combination

Research Society and Development ◽

10.33448/rsd-v10i4.14150 ◽

2021 ◽

Vol 10 (4) ◽

pp. e33910414150

Author(s):

Lucas Dutra Zani da Silva Souza ◽

Ariana Ferrari ◽

Daniele Fernanda Felipe

Keyword(s):

Synergistic Effect ◽

Antioxidant Properties ◽

Natural Antioxidants ◽

Antioxidant Property ◽

Antioxidant Potential ◽

Antioxidant Compounds ◽

Pfaffia Glomerata ◽

Therapeutic Benefits ◽

Dpph Method ◽

Arrabidaea Chica

Several therapeutic benefits of medicinal plants are attributed to antioxidant properties, since antioxidant compounds play an important role in the prevention of various diseases and aging. Many products have synergistic action from combinations of plant extracts that contain natural antioxidants. The present study aimed to evaluate the synergistic effect of Pfaffia glomerata extract with Arrabidaea chica extract for antioxidant action. In the study the extract of the inflorescences of P. glomerata and the extract of the leaves of A. chica were used. The synergism between P. glomerata extract and A. chica extract was evaluated using the ratio between experimental and theoretical antioxidant activity, which was determined using the DPPH method and the potential total reactive antioxidant method (TRAP). The combination of P. glomerata extract and A. chica extract showed a synergistic effect, in which the antioxidant property of this combination was substantially superior to the sum of the individual antioxidant effects. This demonstrates that the combined extracts can be important for the development of products that have a high antioxidant potential.

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The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed (Nigella sativaL.) Oil

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/6273817 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 16

Author(s):

Nameer Khairullah Mohammed ◽

Mohd Yazid Abd Manap ◽

Chin Ping Tan ◽

Belal J. Muhialdin ◽

Amaal M. Alhelli ◽

...

Keyword(s):

Antioxidant Activity ◽

Thermal Behavior ◽

Nigella Sativa ◽

Antioxidant Properties ◽

Natural Antioxidants ◽

Extraction Methods ◽

Total Phenolic ◽

Antioxidant Power ◽

Black Seeds ◽

High Level

TheNigella sativaL. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine.N.sativaseeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in theNigella sativaoil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE.

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Synthesis and Characterization of Green Carbon Dots for Scavenging Radical Oxygen Species in Aqueous and Oil Samples

Antioxidants ◽

10.3390/antiox9111147 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1147

Author(s):

Clarissa Murru ◽

Rosana Badía-Laíño ◽

Marta Elena Díaz-García

Keyword(s):

Carbon Dots ◽

Metal Ion ◽

Radical Scavenging Activity ◽

Aqueous Media ◽

Antioxidant Properties ◽

Carbon Sources ◽

Radical Scavenging ◽

Radical Oxygen Species ◽

Ion Sensing ◽

Bio Imaging

Carbon dots (CDs) due to their unique optical features, chemical stability and low environmental hazard are applied in different fields such as metal ion sensing, photo-catalysis, bio-imaging and tribology, among others. The aims of the present research were to obtain CDs from vegetable wastes (tea and grapes) as carbon sources and to explore their potential properties as radical scavengers. CDs from glutathione/citric acid (GCDs) were synthetized for comparison purposes. The CDs were investigated for their chemical structure, morphology, optical and electronical properties. The antioxidant activity has been explored by DPPH and Folin-Ciocelteau assays in aqueous media. Due to their solubility in oil, the CDs prepared from tea wastes and GCDs were assayed as antioxidants in a mineral oil lubricant by potentiometric determination of the peroxide value. CDs from tea wastes and GCDs exhibited good antioxidant properties both in aqueous and oil media. Possible mechanisms, such as C-addition to double bonds, H-abstraction and SOMO-CDs conduction band interaction, were proposed for the CDs radical scavenging activity. CDs from natural sources open new application pathways as antioxidant green additives.

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Antioxidant Activity of Spray-Dried Extracts of Psidium guajava Leaves

Journal of Food Research ◽

10.5539/jfr.v7n4p141 ◽

2018 ◽

Vol 7 (4) ◽

pp. 141 ◽

Cited By ~ 1

Author(s):

Maurette R. V. Fernandes ◽

Luciana M. Kabeya ◽

Cláudia R. F. Souza ◽

Adna P. Massarioli ◽

Severino M. Alencar ◽

...

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Natural Antioxidants ◽

Psidium Guajava ◽

Absorption Capacity ◽

Antioxidant Power ◽

Dpph Method ◽

Dpph Scavenging ◽

Spray Dried

Antioxidants from Psidium guajava leaves were extracted with 70% ethanol in water. The extractive solution was concentrated and submitted to spray drying in the presence of technological adjuvants at a proportion of 8 % wet base. Three distinct technological adjuvants were evaluated: β-cyclodextrin (βCD-80), maltodextrin DE10:Aerosil® (MA-80 - 7:1), and maltodextrin DE10:Encapsia®:Aerosil® (MDEA-80 - 5:2:1). The antioxidant activity of the concentrated extract and spray-dried powders was assessed by three antioxidant assays, namely: the2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP), and oxygen radical absorption capacity (ORAC). The 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging capacity was determined previously. The spray-dried powders exhibited strong antioxidant activity (IC50 value = 7.96 to 9.76 μg/mL – DPPH method; 3,125.1 to 3,406.0 μM TE/g dry weight – ABTS method; 4,210 to 4,540 μM FeSO4 E/g – FRAP method; 1,820-2,020 μM TE/g – ORAC method). The technological adjuvants did not significantly interfere with the antioxidant activity of the dried products, regardless the type of antioxidant assay used. The results here reported strongly evidenced that the concentrated and spray-dried extracts of Psidium guajava are rich sources of natural antioxidants with potential application in food, pharmaceutical, and cosmeceutical products.

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The Contribution of Carotenoids, Phenolic Compounds, and Flavonoids to the Antioxidative Properties of Marine Microalgae Isolated from Mediterranean Morocco

Molecules ◽

10.3390/molecules24224037 ◽

2019 ◽

Vol 24 (22) ◽

pp. 4037 ◽

Cited By ~ 9

Author(s):

Imane Haoujar ◽

Francesco Cacciola ◽

Jamal Abrini ◽

Domenica Mangraviti ◽

Daniele Giuffrida ◽

...

Keyword(s):

Antioxidant Activity ◽

Sea Water ◽

Antioxidant Properties ◽

Natural Antioxidants ◽

In Vitro Assays ◽

Ferrous Ions ◽

Nannochloropsis Gaditana ◽

Antioxidant Power ◽

Ferric Reducing Antioxidant Power

This study aimed to investigate the potential of four sea water microalgae, isolated and cultivated at M′diq Bay in Morocco, as a new source of natural antioxidants. These microalgae belong to different classes, including Phaedactylium tricornitum (Bacillariophyceae), Nannochloropsis gaditana (Eustigmatophyceae), Nannochloris sp (Trebouxiophyceae), and Tetraselmis suecica (Chlorodendrophycea). The antioxidant properties were screened by the use of in vitro assays, namely 2,2-difenil-1-picrylhydrazyl, Ferric reducing antioxidant power, and Ferrous ions chelating activity, and compoundidentification was carried out in methanol and acetone extracts of both dried and fresh microalgae biomass by HPLC–PDA–MS analysis. Among the investigated microalgae, Phaedactylium tricornutum was the richest one regarding its carotenoid (especially all-E-fucoxanthin) and phenolic (especially protocatechuic acid) contents, as well as antioxidant activity (65.5%), followed by Nannochloris sp, Tetraselmis suicica, and Nannochloropsis gaditana, with antioxidant activity of 56.8%, 54.9%, and 51.1%, respectively.

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Phenolic Composition, Antioxidant Capacity and Antibacterial Activity of White Wormwood (Artemisia herba-alba)

Plants ◽

10.3390/plants10010164 ◽

2021 ◽

Vol 10 (1) ◽

pp. 164

Author(s):

Muthanna J. Mohammed ◽

Uttpal Anand ◽

Ammar B. Altemimi ◽

Vijay Tripathi ◽

Yigong Guo ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Properties ◽

Soxhlet Extraction ◽

Hydroxyl Group ◽

Frap Assay ◽

Antioxidant Power ◽

Antimicrobial Effects ◽

Dpph Method ◽

Ic50 Values ◽

Fraction I

Artemisia herba-alba Asso. (Wormwood) is a wild aromatic herb that is popular for its healing and medicinal effects and has been used in conventional as well as modern medicine. This research aimed at the extraction, identification, and quantification of phenolic compounds in the aerial parts of wormwood using Soxhlet extraction, as well as characterizing their antimicrobial and anitoxidant effects. The phenolic compounds were identified in different extracts by column chromatography, thin layer chromatography (TLC), and high performance liquid chromatography. Five different fractions, two from ethyl acetate extraction and three from ethanolic extraction were obtained and evaluated further. The antimicrobial activity of each fractions was evaluated against two Gram-positive (Bacillus cereus and Staphylococcus aureus) and two Gram-negative microorganisms (Escherichia coli and Proteus vulgaris) using the disc-diffusion assay and direct TLC bioautography assay. Fraction I inhibited B. cereus and P. vulgaris, Fraction II inhibited B. cereus and E. coli, Fraction III inhibited all, except for P. vulgaris, while Fractions IV and V did not exhibit strong antimicrobial effects. Their antioxidant capabilities were also measured by calculating their ability to scavenge the free radical using DPPH method and the ferric reducing antioxidant power (FRAP) assay. Ethanolic fractions III and V demonstrated excellent antioxidant properties with IC50 values less than 15.0 μg/mL, while other fractions also had IC50 values less than 80.0 μg/mL. These antioxidant effects were highly associated with the number of phenolic hydroxyl group on the phenolics they contained. These extracts demonstrated antimicrobial effects, suggesting the different phenolic compounds in these extracts had specific inhibitory effects on the growth of each bacteria. The results of this study suggested that the A. herba-alba can be a source of phenolic compounds with natural antimicrobial and antioxidant properties which can be used for potential pharmaceutical applications.

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Comparative analysis of the chemical composition and antioxidant properties of different types of tea as a raw material for the production of tea extracts

Proceedings of the Voronezh State University of Engineering Technologies ◽

10.20914/2310-1202-2018-2-249-255 ◽

2018 ◽

Vol 80 (2) ◽

pp. 249-255 ◽

Cited By ~ 1

Author(s):

D. F. Valiulina ◽

N. V. Makarova ◽

D. V. Budylin

Keyword(s):

Green Tea ◽

Antiradical Activity ◽

Antioxidant Properties ◽

Total Content ◽

Natural Antioxidants ◽

Raw Material ◽

Soluble Solids ◽

Restoring Force ◽

Dpph Method ◽

Phenolic Substances

One of the most frequently consumed sources of natural antioxidants is tea. High content of phenolic substances in it, makes it an excellent functional drink. At present, interest in tea is great not only as an independent drink, but also as a raw material for the production of tea extracts. The study was conducted on extracts obtained from samples of leaf tea of foreign and Russian origin in the following parameters: the content of soluble solids in tea extracts; total content of phenolic substances; total content of flavonoids; total content of tannins; antiradical activity by the DPPH method; restoring force by the method of FRAP. During the study of tea species, different in the way of processing and in the region of origin, it was determined that the leader among the presented teas is the white tea of Chinese origin, which has the highest antiradical activity and the highest content of tannins. Comparing green tea from China and Russia, it can be concluded that the content of individual groups of substances varies slightly against the background of slightly higher anti-radical activity of Chinese tea. In the group of black teas, the Indian "Harmutti" tea is in the lead by the results of the test, significantly exceeding tea from the Krasnodar Territory by anti-radical activity and the total content of phenolic substances. Thus, promising types of tea for the prevention of diseases (diabetes, atherosclerosis, hypertension, Alzheimer's disease), arising as a consequence of oxidative stress, are tea white Chinese and green tea Krasnodar.

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