Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds

The extraction of phenolic compounds from olive leaves was optimized using three glycerol-based deep eutectic solvents (DESs) with lysine, proline, and arginine. A three-level Box–Behnken design was used to examine the influence of the liquid/solid ratio, concentration of DESs, and extraction temperature on the yield of the extraction process. A second-order polynomial model was used for predicting the polyphenol extraction yield. The optimal predicted conditions were used for extractions and they provided the highest total phenol yields with the glycerol–lysine exhibiting the best performance. Quantification of tyrosol, hydroxytyrosol, oleuropein, luteolin-7-O-glucoside, and rutin in the extracts showed high content in tyrosol in all DESs, particularly with glycerol–lysine and relatively similar contents with other studies for the other phenolic compounds. Finally, a linear relationship between tyrosol content and the total phenolic content of the extracts was observed.

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Subcritical Water Extraction of Antioxidants from Curculigo latifolia Root

Journal of Chemistry ◽

10.1155/2019/8047191 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Nur Athirah Zabidi ◽

Nur Akmal Ishak ◽

Muhajir Hamid ◽

Siti Efliza Ashari

Keyword(s):

Phenolic Compounds ◽

Subcritical Water ◽

Extraction Yield ◽

Water Extraction ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Root Extract ◽

Extraction Temperature ◽

Subcritical Water Extraction ◽

Trolox Equivalent

This study aims to extract antioxidant compounds from Curculigo latifolia root by subcritical water extraction (SWE). The influence of extraction temperature and time on the antioxidant activity of C. latifolia root extract was investigated in terms of extraction yield, total phenolic content (TPC), total flavonoid content (TFC), 2, 2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The highest extraction yield (36.5%), TPC (92.55 mg GAE/g), TFC (13.26 mg RE/g), and antioxidant activities by ABTS (66.8 mg trolox equivalent/g sample) and DPPH (128.7 mg trolox equivalent/g sample) were detected at 180°C and 30 minutes extraction time. Based on the results, the optimum condition was selected at 180°C and 30 minutes of extraction. The sample was screened by using liquid chromatography-mass spectrometry (LC-MS) analysis where the phenolic compounds detected were structured based on their [M-H]-peak. The detected phenolic compounds were monobenzone, hydroquinone, phloridzin, pomiferin, mundulone, scandenin, and dimethyl caffeic acid. According to these findings, SWE is a promising, environmentally friendly, and efficient technology in the exploitation of natural products for the development of food and nutraceutical commodities.

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Effect of Time, Solvent-Solid Ratio, Ethanol Concentration and Temperature on Extraction Yield of Phenolic Compounds From Olive Leaves

Engineering, Technology & Applied Science Research ◽

10.48084/etasr.1983 ◽

2018 ◽

Vol 8 (2) ◽

pp. 2805-2808

Author(s):

N. Elboughdiri

Keyword(s):

Phenolic Compounds ◽

Ethanol Concentration ◽

Extraction Yield ◽

Extraction Time ◽

Total Phenolic ◽

Optimal Conditions ◽

Total Phenolic Compounds ◽

Olive Leaves ◽

Factors Affecting ◽

Solid Ratio

This study aims to determine the factors affecting the process of extraction of phenolic compounds from olive leaves. Two methods of extraction were used in this work and different tests were implemented with the aim of optimizing the recovery of phenolic compounds from olive leaves. The factors considered were the extraction time, temperature, solvent-solid ratio and the ethanol concentration. Obtained results show that the recommended optimal conditions for the extraction of the total phenolic compounds from olive leaves were found at 40°C with a solvent/solid ratio of 30:1 and ethanol concentration of 80% (v/v) when the dried olive leaves are stored at -21°C at the beginning of the experiments.

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Effect of Extraction Process on Extraction Yeild, Total Polyphenol Content and Antioxidant Activity of Jasminum Subtriplinerve

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.814.527 ◽

2019 ◽

Vol 814 ◽

pp. 527-531

Author(s):

Trieu Tuan Anh ◽

Luu Tu Hao ◽

Long Giang Bach ◽

Duy Chinh Nguyen ◽

Tri Duc Lam

Keyword(s):

Antioxidant Activity ◽

Extraction Process ◽

Extraction Yield ◽

Total Phenolic ◽

Extraction Temperature ◽

Total Phenolic Compounds ◽

Polyphenol Content ◽

Total Polyphenol ◽

Herbal Plants ◽

Optimized Conditions

Jasminum Subtriplinerve Blume (Oleaceae) is herbal plants widely a tea for weight loss, stimulates milk glands. Extract product it easy used yet had little research. This study was carried out to evaluate the effect of extracting conditions such as water/material ratio, extraction temperature and extracting time on extracting yield, polyphenol content and antioxidant activity of J.subtriplinerve extract. The total phenolic compounds were determined according to the Folin–Ciocalteu method. Extraction yield (0.22g/100 g) was obtained at water/material ratio 15:1 (ml/g), 40°C and 4 hours extraction time. Under these optimized conditions, the polyphenol content of was 2640.4 μg/1g extract. And the extract can harvest 46.11 % of DPPH (30 μg/ mL) at 10 000 μg/ml concentration

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Susam Kepeğinden Protein ve Antioksidan Özellikli Maddelerin Geri Kazanım Potansiyelinin Bazı Temel Bileşim Analizleri ile İncelenmesi

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v7i4.624-630.2347 ◽

2019 ◽

Vol 7 (4) ◽

pp. 624 ◽

Cited By ~ 1

Author(s):

Ahmet Görgüç ◽

Fatih Mehmet Yılmaz

Keyword(s):

Phenolic Compounds ◽

Protein Extraction ◽

Animal Feed ◽

Sesame Seed ◽

Extraction Yield ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Dry Processing ◽

Wet Processing ◽

Processing Line

Sesame bran is an industrial waste which is either not currently evaluated technologically or has limited use as animal feed. In this study, recovery potentials of protein and antioxidant compounds were investigated from three different sesame brans which are emerge as waste from dry and wet processing lines of the sesame - an intensively used input by roasted sesame seed, tahini and tahini halva plants especially in our country. In this context, protein extraction by alkaline method was conducted on brans obtained from three different processing lines (Dry line I, Dry line II and Wet line II). In addition, effects of aqueous solutions (70%, v/v) of acetone, ethanol and methanol on the extraction of phenolic compounds from sesame brans were investigated. Dry processing line sesame bran was found to have lower protein content than wet processing line bran; however, higher protein extraction yield was obtained by dry processing line bran. Phenolic compound extracts of dry and wet processing line sesame brans were found to have higher total phenolic content and antioxidant capacity values (DPPH and ABTS assays) than protein extracts obtained by alkaline method. It was determined that the most effective solvent for the extraction of phenolic compounds from sesame bran was 70% acetone solution; and the results of the ethanol and methanol extracts used as solvents were not significantly different.

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Oil Extraction and Evaluation from Yellow Horn Using a Microwave-Assisted Aqueous Saline Process

Molecules ◽

10.3390/molecules24142598 ◽

2019 ◽

Vol 24 (14) ◽

pp. 2598 ◽

Cited By ~ 4

Author(s):

Yulong Huang ◽

Zhenxiong Yin ◽

Jie Guo ◽

Fengxia Wang ◽

Ji Zhang

Keyword(s):

Seed Oil ◽

Extraction Process ◽

Oil Extraction ◽

Extraction Yield ◽

Gas Chromatography Mass Spectrometry ◽

Extraction Temperature ◽

Box Behnken Design ◽

Microwave Assisted ◽

Assisted Extraction ◽

Nacl Concentration

This study investigates an aqueous salt process (ASP) combined with microwave-assisted extraction (MAE) for the seed oil extraction from yellow horn (Xanthoceras sorbifolium Bunge). The NaCl concentration in the oil extraction process affected the oil extraction yield. Box–Behnken design (BBD) and response surface methodology (RSM) were used to optimize the extraction process. The optimal operating parameters were: 24 g/L NaCl, 300 W microwave power, 4:1 water to material ratio, an 80 min extraction time, and 45 °C extraction temperature. The chemical composition of the extracted seed oil was analyzed using gas chromatography–mass spectrometry (GC-MS). This extraction technique for yellow horn seed oil provided high throughput and high-quality oil. The present research offers a kind of green extraction method for edible oil in the food industry.

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Optimization of Ultrasound Assisted Extraction of Bioactive Compounds from Apple Pomace

Molecules ◽

10.3390/molecules26133783 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3783

Author(s):

Itziar Egüés ◽

Fabio Hernandez-Ramos ◽

Iván Rivilla ◽

Jalel Labidi

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Extraction Yield ◽

Apple Pomace ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Maximum Extraction ◽

Ultrasound Technology

In the present work, the optimization of the extraction of antioxidant compounds from apple pomace using ultrasound technology as an environmentally friendly and intensification process was developed. Different sonication powers, extraction temperatures and extraction times were studied and their influence on extraction yield and characteristics of the extracted samples (total phenolic compounds, flavonoid content and antioxidant capacity) are presented. The elaborated experimental design and the analysis of Pareto and response surface diagrams allowed us to determine the optimal extraction conditions. The conditions that allow the maximum extraction of phenolic compounds were found at 20 min, 90 °C and 50% ultrasound amplitude. Nevertheless, at these conditions, the antioxidant capacity measured by DPPH decreased in the extracted samples.

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Green Extraction of Phenolic Compounds from Lotus Seedpod (Receptaculum Nelumbinis) Assisted by Ultrasound Coupled with Glycerol

Foods ◽

10.3390/foods10020239 ◽

2021 ◽

Vol 10 (2) ◽

pp. 239

Author(s):

Nina Bao ◽

Di Wang ◽

Xizhe Fu ◽

Hujun Xie ◽

Guizhen Gao ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Extraction Yield ◽

Total Phenolic ◽

Glycerol Concentration ◽

Extraction Temperature ◽

Characteristic Functional ◽

Antioxidant Power ◽

Ultrasonic Time

Lotus Receptaculum Nelumbinis has been sparking wide research interests due to its rich phenolic compounds. In the present work, ultrasonic-assisted extraction coupled with glycerol was employed to extract phenolic compounds from Receptaculum Nelumbinis and the process was optimized using a response surface methodology with Box-Behnken design (BBD). The optimal conditions for the total phenolic content (TPC) extract were obtained: glycerol concentration of 40%, an extraction temperature of 66 °C, ultrasonic time of 44 min, and the solvent-to-solid ratio of 55 mL/g. Under these optimum extraction conditions, the extraction yield of TPC was 92.84 ± 2.13 mg gallic acid equivalents (GAE) /g. Besides, the antioxidant activities demonstrated the ability of free radical scavenging by four different methods that included 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing activity (RA) were 459.73 ± 7.07, 529.97 ± 7.30, 907.61 ± 20.28, and 983.66 ± 11.80 μmol TE/g, respectively. Six phenolic compounds were identified by ultra-high pressure liquid chromatography combined with triple-time-of-flight mass spectrophotometry (UPLC-Triple-TOF/MS) from the extracts. Meanwhile, Fourier transform infrared (FTIR) was conducted to identify the characteristic functional groups of the extracts and thus reflected the presence of polyphenols and flavonoids. Scanning electron microscopy (SEM) illustrated the microstructure difference of four treatments, which might explain the relationships between antioxidant activities and the structures of phenolic compounds.

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DES Based Efficient Extraction Method for Bioactive Coumarins from Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav.

Separations ◽

10.3390/separations9010005 ◽

2021 ◽

Vol 9 (1) ◽

pp. 5

Author(s):

Ting Wang ◽

Qian Li

Keyword(s):

Choline Chloride ◽

Radical Scavenging ◽

Deep Eutectic Solvents ◽

Extraction Yield ◽

Molar Ratio ◽

Angelica Dahurica ◽

Extraction Temperature ◽

Solid Ratio ◽

Before And After ◽

Ultrasonic Assisted

In this study, a simple and environmentally friendly method was developed for the extraction of seven active coumarins from Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav.(A. dahurica) based on deep eutectic solvents (DESs). Among the 16 kinds of DES based on choline chloride, the DES system with the molar ratio of choline chloride, citric acid, and water as 1:1:2 had the best extraction effect. Ultrasonic-assisted response surface methodology (RSM) was used to investigate the optimal extraction scheme. The results showed that the optimal extraction conditions were a liquid–solid ratio of 10:1 (mL/g), an extraction time of 50 min, an extraction temperature of 59.85 °C, and a moisture content of 49.28%. Under these conditions, the extraction yield reached 1.18%. In addition, scanning electron microscopy (SEM) was used to observe the degree of powder fragmentation before and after extraction with different solvents. The cells of A. dahurica medicinal materials obtained by DES ultrasonic-assisted treatment were the most seriously broken, indicating that DES had the highest efficiency in the treatment of A. dahurica. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) DPPH radical scavenging model was used to evaluate the biological activity of DES extract. The results showed that DES extract had better scavenging ability of DPPH free radical. Therefore, DES is a green solvent suitable for extracting coumarin compounds of A. dahurica, with great potential to replace organic solvents.

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Aqueous Enzymatic Extraction of Buriti (Mauritia Flexuosa) Oil: Yield and Antioxidant Compounds

The Open Food Science Journal ◽

10.2174/1874256401911010009 ◽

2019 ◽

Vol 11 (1) ◽

pp. 9-17 ◽

Cited By ~ 1

Author(s):

Jezica P.P. Silva ◽

Antonio M.C. Rodrigues ◽

Luiza H.M. Silva

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Extraction Process ◽

Aqueous Extraction ◽

Carotenoid Content ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Total Phenolic Compounds ◽

Buriti Oil ◽

Aqueous Extraction Process

Introduction: Enzyme-assisted aqueous extraction is considered an emerging green technique that has been applied to different oilseeds. Objective: This study aimed to study the enzymatic aqueous extraction process of buriti oil using a central composite rotatable design (CCRD) combined with the response surface methodology aiming to obtain higher yield and antioxidant compounds in the oil. Methods: The study was carried out in two steps. The first assessed the efficiency of different enzymes (cellulase, pectinase, and protease) and the variables of greater influence in the extraction process, being conducted for each enzyme a CCRD design. The second step was carried out with the enzyme that showed the best performance on the extraction yield, changing the experimental bands of the variables that had greater significance in the first step, with the goal of broadening the spectrum of study. Were also evaluated in this step, total carotenoids, total phenolic compounds, and the antioxidant capacity of the oils extracted. Results: In the first experiment, cellulase gave the highest yield, while the most significant variables were temperature and time. For the second design, performed with cellulase, were defined as optimal operating conditions at 55 °C temperature, 2% enzyme concentration and 6 hours extraction. For these conditions, the yield obtained was 76.5%, with total carotenoid concentration of 3,119.5 µg β-carotene.g-1. Analysis of variance was performed and showed the significance of the regression and non-significance of the lack-of-fit (p<0.05). The coefficients of determination of the yield and carotenoid content were 95.6% and 94.5%, respectively. The highest value of total phenolic compounds determined for buriti oil in this study was 254 ± 5 µg GAE.g-1 oil, while for the antioxidant capacity was 218.0 ± 0.3 µmol Trolox.g-1 oil. Conclusion: The enzymatic aqueous extraction process is viable for buriti oil and produced oils with high concentrations of antioxidant compounds.

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Extraction yield, antioxidant activity and total phenolic content of Mimusops elengi L. fruit

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/922/1/012021 ◽

2021 ◽

Vol 922 (1) ◽

pp. 012021

Author(s):

Z Zaidiyah ◽

M G A Ghifari ◽

Y Abubakar

Keyword(s):

Antioxidant Activity ◽

Extraction Process ◽

Extraction Yield ◽

Effect Of Temperature ◽

Total Phenolic ◽

Extraction Temperature ◽

Fruit Extract ◽

Phenolic Contents ◽

Total Phenolic Contents ◽

Mimusops Elengi

Abstract Mimusops elengi L. fruit or known as Bakul fruit is believed to have a lot of benefit due to its flavonoid group compound that can have antioxidant effects. This study aimed to determine the effect of temperature and ratio between material and solvent in the extraction process on the antioxidant activity and content of phenolic compounds in Mimusops elengi L. fruit extract. This research used factorial completely randomized design with 2 factors : extraction temperature (45¼C, 60¼C, and 75¼C) and ratio of the material and solvent (1:4, 1:6, and 1:8) with 3 replications. The extraction yield, total phenolic contents and antioxidant activity of Mimusops elengi L. fruit extract were examined. Furthermore, the application of extraction temperature in 75¼C with ratio 1:8 material to solvent (w/v) has the highest extraction yield, total phenolic contents and antioxidant activity compared to others treatments.

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