scholarly journals CFD-Based and Experimental Hydrodynamic Characterization of the Single-Use Bioreactor XcellerexTM XDR-10

2022 ◽  
Vol 9 (1) ◽  
pp. 22
Author(s):  
Diana Kreitmayer ◽  
Srikanth R. Gopireddy ◽  
Tomomi Matsuura ◽  
Yuichi Aki ◽  
Yuta Katayama ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Mixing Time ◽  
Vortex Formation ◽  
Operating Conditions ◽  
Impeller Speed ◽  
Oxygen Mass Transfer ◽  
Hydrodynamic Conditions ◽  
Single Use ◽  
Selection Of ◽  
Surface Vortex

Understanding the hydrodynamic conditions in bioreactors is of utmost importance for the selection of operating conditions during cell culture process development. In the present study, the two-phase flow in the lab-scale single-use bioreactor XcellerexTM XDR-10 is characterized for working volumes from 4.5 L to 10 L, impeller speeds from 40 rpm to 360 rpm, and sparging with two different microporous spargers at rates from 0.02 L min−1 to 0.5 L min−1. The numerical simulations are performed with the one-way coupled Euler–Lagrange and the Euler–Euler models. The results of the agitated liquid height, the mixing time, and the volumetric oxygen mass transfer coefficient are compared to experiments. For the unbaffled XDR-10, strong surface vortex formation is found for the maximum impeller speed. To support the selection of suitable impeller speeds for cell cultivation, the surface vortex formation, the average turbulence energy dissipation rate, the hydrodynamic stress, and the mixing time are analyzed and discussed. Surface vortex formation is observed for the maximum impeller speed. Mixing times are below 30 s across all conditions, and volumetric oxygen mass transfer coefficients of up to 22.1 h−1 are found. The XDR-10 provides hydrodynamic conditions which are well suited for the cultivation of animal cells, despite the unusual design of a single bottom-mounted impeller and an unbaffled cultivation bioreactor.

scholarly journals Power Consumption, Mixing Time, and Oxygen Mass Transfer in a Gas-Liquid Contactor Stirred with a Dual Impeller for Different Spacing

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Hayder Mohammed Issa
Keyword(s):  
Mass Transfer ◽  
Power Consumption ◽  
Transfer Coefficient ◽  
Mass Transfer Coefficient ◽  
Mixing Time ◽  
Oxygen Mass Transfer ◽  
System A ◽  
Phase Water ◽  
Pharmaceutical Production ◽  
Slight Alteration

Multiple or dual impellers are widely implemented in stirred contactors used in various biological processes like fermentation, water treatment, and pharmaceutical production. The spacing between impellers is considered as a crucial factor in designing of these types of contactors resulting in variation of oxygen mass transfer, mixing time, or power consumption for such biological system. A study of three parts was conducted to characterize the effect of the spacing between impellers on the most important parameters that related to biological contactor performance: oxygen mass transfer coefficientklafrom the gas phase (air) to the liquid phase (water), mixing time, and power consumption for different operating rotational speeds (1.67–3.33 rps) and for three different spacing positions. The used impellers system in the study is a dual impeller system which consists of an inverted and bladed rotated cone (IBRC) and a pitched-blade up-flow propeller (PBPU). The experimental results showed that the shorter spacing (the lower PBPU in a higher position) is more convenient, as the achieved oxygen mass transfer coefficient has showed an improvement in its values with lower mixing time and with a slight alteration in power consumption.

scholarly journals Does the BioBLU 0.3f single-use scale to the BioFlo® 320 reuseable bioreactor on a matched volumetric oxygen mass transfer coefficient?

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Williams Olughu ◽  
Duncan Galbraith ◽  
Cillian Paget ◽  
Steve Ruscoe ◽  
Josh Smith ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Transfer Coefficient ◽  
Mass Transfer Coefficient ◽  
High Cell Density ◽  
Scale Up ◽  
Limiting Factor ◽  
Oxygen Mass Transfer ◽  
Addition Method ◽  
Essential Parameter ◽  
Single Use

AbstractThe volumetric oxygen mass transfer coefficient ($$k_{l} a$$ k l a ) is an essential parameter in aerobic high-cell density fermentation where the availability of oxygen to growing microorganisms is a limiting factor. Bioprocess teams looking to scale-up/down between the Eppendorf BioBLU 0.3f single-use vessel and the BioFlo® 320 reusable vessel bioreactors may find it challenging using a matched $$k_{l} a$$ k l a . The maximum $$k_{l} a$$ k l a of the BioFlo® 320 reusable bioreactor was 109 h−1, which was approximately twice that of the BioBLU 0.3f single-use vessel. The results here show no overlap in $$k_{l} a$$ k l a values when both bioreactors were compared and thus conclude that scalability based on $$k_{l} a$$ k l a is not viable. The maximum $$k_{l} a$$ k l a of the Eppendorf BioBLU 0.3f single-use reported here was 47 h−1 compared to that of the manufacturer’s value of 2500 h−1, indicating a 53-fold difference. This discrepancy was attributed to the incompatible sulfite addition method used by the manufacturer for estimation.

Modeling mass transfer and substrate utilization in the boundary layer of biofilm systems

1998 ◽  
Vol 37 (4-5) ◽  
pp. 139-147 ◽  
Author(s):  
Harald Horn ◽  
Dietmar C. Hempel
Keyword(s):  
Boundary Layer ◽  
Mass Transfer ◽  
Reynolds Number ◽  
Film Theory ◽  
Substrate Utilization ◽  
The Other ◽  
Concentration Profiles ◽  
Hydrodynamic Conditions ◽  
Time Axis ◽  
Transfer Coefficients

The use of microelectrodes in biofilm research allows a better understanding of intrinsic biofilm processes. Little is known about mass transfer and substrate utilization in the boundary layer of biofilm systems. One possible description of mass transfer can be obtained by mass transfer coefficients, both on the basis of the stagnant film theory or with the Sherwood number. This approach is rather formal and not quite correct when the heterogeneity of the biofilm surface structure is taken into account. It could be shown that substrate loading is a major factor in the description of the development of the density. On the other hand, the time axis is an important factor which has to be considered when concentration profiles in biofilm systems are discussed. Finally, hydrodynamic conditions become important for the development of the biofilm surface when the Reynolds number increases above the range of 3000-4000.

scholarly journals Two-Phase Flow Mass Transfer Analysis of Airlift Pump for Aquaculture Applications

Fluids ◽  
2021 ◽  
Vol 6 (6) ◽  
pp. 226
Author(s):  
Rashal Abed ◽  
Mohamed M. Hussein ◽  
Wael H. Ahmed ◽  
Sherif Abdou
Keyword(s):  
Mass Transfer ◽  
Oxygen Transfer ◽  
Transfer Rate ◽  
Two Phase Flow ◽  
Oxygen Transfer Rate ◽  
Flow Patterns ◽  
Oxygen Mass Transfer ◽  
Phase Flow ◽  
Two Phase ◽  
Airlift Pump

Airlift pumps can be used in the aquaculture industry to provide aeration while concurrently moving water utilizing the dynamics of two-phase flow in the pump riser. The oxygen mass transfer that occurs from the injected compressed air to the water in the aquaculture systems can be experimentally investigated to determine the pump aeration capabilities. The objective of this study is to evaluate the effects of various airflow rates as well as the injection methods on the oxygen transfer rate within a dual injector airlift pump system. Experiments were conducted using an airlift pump connected to a vertical pump riser within a recirculating system. Both two-phase flow patterns and the void fraction measurements were used to evaluate the dissolved oxygen mass transfer mechanism through the airlift pump. A dissolved oxygen (DO) sensor was used to determine the DO levels within the airlift pumping system at different operating conditions required by the pump. Flow visualization imaging and particle image velocimetry (PIV) measurements were performed in order to better understand the effects of the two-phase flow patterns on the aeration performance. It was found that the radial injection method reached the saturation point faster at lower airflow rates, whereas the axial method performed better as the airflow rates were increased. The standard oxygen transfer rate (SOTR) and standard aeration efficiency (SAE) were calculated and were found to strongly depend on the injection method as well as the two-phase flow patterns in the pump riser.

Influence of pluronic F68 on oxygen mass transfer

2013 ◽  
Vol 29 (5) ◽  
pp. 1278-1288 ◽  
Author(s):  
Christian Sieblist ◽  
Marco Jenzsch ◽  
Michael Pohlscheidt
Keyword(s):  
Mass Transfer ◽  
Oxygen Mass Transfer ◽  
Pluronic F68

Analysis of Vapor Evaporation Loss in Filling Gasoline into Tank

2011 ◽  
Vol 347-353 ◽  
pp. 372-375 ◽  
Author(s):  
Wei Qiu Huang ◽  
Feng Li ◽  
Shu Hua Zhao ◽  
Jing Zhong
Keyword(s):  
Mass Transfer ◽  
Experimental System ◽  
Pilot Scale ◽  
Convective Transport ◽  
Operating Conditions ◽  
Vapor Concentration ◽  
Air Mass ◽  
Evaporation Loss ◽  
Gas Space ◽  
Gasoline Evaporation

A pilot-scale experimental system of filling gasoline into a tank was built to investigate gasoline vapor-air mass transfer in the tank gas space and the vapor evaporation loss from the tank in different operating conditions. The results showed that the higher the location of filling pipe exit inside the tank, the quicker the speed of the filling gasoline, and the higher the initial vapor concentration in the tank gas space, then the more severe the vapor-air convective transport and the larger the gasoline evaporation loss rates.

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