scholarly journals A Time-Course Study on a Food Contact Material (FCM)-Certified Coating Based on Titanium Oxide Deposited onto Aluminum

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 97
Author(s):  
Alessandro Di Cerbo ◽  
Andrea Mescola ◽  
Giuseppe Rosace ◽  
Valentina Trovato ◽  
Roberto Canton ◽  
...  

Aluminum is the second most widely used metal worldwide. It is present as an additive in cosmetics, pharmaceuticals, food, and food contact materials (FCM). In this study, we confirm the bactericidal effect of a special anodizing method, based on TiO2 nanoparticles (DURALTI®) deposited on aluminum disks with different roughness and subjected to two sanitizing treatments: UV and alcohol 70%. Consequently, we perform a time-course evaluation against both Gram-negative and Gram-positive bacteria to better frame the time required to achieve the best result. Approximately 106 CFU/mL of Escherichia coli ATCC 25922; Salmonella Typhimurium ATCC 1402; Yersinia enterocolitica ATCC 9610; Pseudomonas aeruginosa ATCC 27588; Staphylococcus aureus ATCC 6538; Enterococcus faecalis ATCC 29212; Bacillus cereus ATCC 14579 and Listeria monocytogenes NCTT 10888 were inoculated onto each aluminum surface and challenged with UV and alcohol 70% at 0, 15”, 30”, 1′, 5′, 15′, 30′, 1, 2, 4 and 6 h. DURALTI® coating already confirmed its ability to induce a 4-logarithmic decrease (from 106 to 102 CFU/mL) after 6 h. Once each sanitizing treatment was applied, an overall bacterial inhibition occurred in a time ranging from 15′′ to 1′. The results are innovative in terms of preventing microbial adhesion and growth in the food industry.

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 901
Author(s):  
Alessandro Di Cerbo ◽  
Giuseppe Rosace ◽  
Stefano Rea ◽  
Roberta Stocchi ◽  
Julio Cesar Morales-Medina ◽  
...  

One of the most-used food contact materials is stainless steel (AISI 304L or AISI 316L), owing to its high mechanical strength, cleanability, and corrosion resistance. However, due to the presence of minimal crevices, stainless-steel is subject to microbial contamination with consequent significant reverb on health and industry costs due to the lack of effective reliability of sanitizing agents and procedures. In this study, we evaluated the noncytotoxic effect of an amorphous SiOxCyHz coating deposited on stainless-steel disks and performed a time-course evaluation for four Gram-negative bacteria and four Gram-positive bacteria. A low cytotoxicity of the SiOxCyHz coating was observed; moreover, except for some samples, a five-logarithm decrease was visible after 1 h on coated surfaces without any sanitizing treatment and inoculated with Gram-negative and Gram-positive bacteria. Conversely, a complete bacterial removal was observed after 30 s–1 min application of alcohol and already after 15 s under UVC irradiation against both bacterial groups. Moreover, coating deposition changed the wetting behaviors of treated samples, with contact angles increasing from 90.25° to 113.73°, realizing a transformation from hydrophilicity to hydrophobicity, with tremendous repercussions in various technological applications, including the food industry.


2020 ◽  
Vol 145 ◽  
pp. 01024
Author(s):  
Yue Qiu ◽  
Yan Ruan ◽  
Genrong Li

The migration rules of two triazine type of UV absorbents (UV-1577 and UV-1579) from polypropylene (PP) food contact materials to food simulant were investigated. A rapid detection method of UV-1577 and UV-1579 in PP food contact materials was established by ultra-performance convergence chromatography (UPC2). The optimal extraction method of the initial solution was determined, and the pretreatment methods of two UV absorbents in the liquid simulants were optimized. Experiment results showed that the interaction effects between the two UV absorbers and the liquid simulants were weak, and the migration behavior was the most obvious in the fatty simulant of isooctane. At the same temperature, the migration amount of both UV-1577 and UV-1579 increased with the extension of time, and UV-1577 was more prone to migration than UV-1579. Within a certain range, the migration amount of the two UV absorbents increased significantly with the increase of the migration temperature. The higher the temperature was, the shorter the time required to reach the equilibrium.


1990 ◽  
Vol 258 (1) ◽  
pp. R99-R103
Author(s):  
M. Leite ◽  
W. N. Suki

The V2-selective analogue of arginine vasopressin (AVP), dDAVP, has been used to distinguish between the effects of V1- and V2-receptor activation by AVP in different cell types of the kidney. Based on studies showing different effects of AVP and dDAVP on prostaglandin secretion, and also on cytosolic Ca2+, we designed a comparative time-course study of both agonists on rabbit microdissected cortical collecting tubules (CCT) microperfused in vitro at 38 degrees C. Plots of the effects of AVP (10 microU/ml or 2.2 x 10(-11) M and 100 microU/ml or 2.2 x 10(-10) M) and dDAVP (10 microU/ml or 0.8 x 10(-11) M) on osmotic water permeability (Pf) at comparable antidiuretic activities, revealed an increase of Pf that was maintained for as long as 170 min of hormone exposure. Also the magnitude of increase in Pf and the time required to achieve the more sustained phase of response were comparable, with no significant difference between the two agonists. These results clearly demonstrate a stable response of rabbit CCT to AVP and dDAVP at physiological temperature, and they reveal no evidence for a difference between the native hormone AVP and its V2 selective analogue on the net hydrosmotic response of the CCT.


2018 ◽  
Vol 22 (12) ◽  
pp. 1249-1254 ◽  
Author(s):  
Mercedes Peltzer ◽  
Juan F. Delgado ◽  
Andres G. Salvay ◽  
Jorge R. Wagner

Hypertension ◽  
1980 ◽  
Vol 2 (4) ◽  
pp. 102-108 ◽  
Author(s):  
A. W. Voors ◽  
L. S. Webber ◽  
G. S. Berenson

2021 ◽  
Vol 38 (3) ◽  
pp. 513-531
Author(s):  
Yoon S. Song ◽  
John L. Koontz ◽  
Rima O. Juskelis ◽  
Eduardo Patazca ◽  
William Limm ◽  
...  

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 591
Author(s):  
Nayely Padilla-Montaño ◽  
Leandro de León Guerra ◽  
Laila Moujir

Species of the Celastraceae family are traditionally consumed in different world regions for their stimulating properties. Celastrol, a triterpene methylene quinone isolated from plants of celastraceas, specifically activates satiety centers in the brain that play an important role in controlling body weight. In this work, the antimicrobial activity and mechanism of action of celastrol and a natural derivative, pristimerin, were investigated in Bacillus subtilis. Celastrol showed a higher antimicrobial activity compared with pristimerin, being active against Gram-positive bacteria with minimum inhibitory concentrations (MICs) that ranged between 0.16 and 2.5 µg/mL. Killing curves displayed a bactericidal effect that was dependent on the inoculum size. Monitoring of macromolecular synthesis in bacterial populations treated with these compounds revealed inhibition in the incorporation of all radiolabeled precursors, but not simultaneously. Celastrol at 3 µg/mL and pristimerin at 10 µg/mL affected DNA and RNA synthesis first, followed by protein synthesis, although the inhibitory action on the uptake of radiolabeled precursors was more dramatic with celastrol. This compound also caused cytoplasmic membrane disruption observed by potassium leakage and formation of mesosome-like structures. The inhibition of oxygen consumption of whole and disrupted cells after treatments with both quinones indicates damage in the cellular structure, suggesting the cytoplasmic membrane as a potential target. These findings indicate that celastrol could be considered as an interesting alternative to control outbreaks caused by spore-forming bacteria.


Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1443
Author(s):  
Arabela Ramírez Carnero ◽  
Antía Lestido-Cardama ◽  
Patricia Vazquez Loureiro ◽  
Letricia Barbosa-Pereira ◽  
Ana Rodríguez Bernaldo de Quirós ◽  
...  

Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are synthetic chemical compounds widely used in different industry fields including food contact materials (FCM), providing resistance to fat and humidity, and non-stick properties. PFAS enter into the food chain directly from the intake of contaminated food or indirectly from the migration of the FCM into the food. This exposure published in different research highlights a public health concern. Therefore, it is necessary to perform analysis of the content of different FCM and evaluate the migration from the FCM under normal conditions of use and storage. This bibliographical review proves that different perfluoroalkyl and polyfluoroalkyl compounds are detected in fast food packaging, microwave popcorn bags, and frying pans, among others. Furthermore, it shows the conditions or factors that favor the migration of the PFAS from the FCM into the food.


2021 ◽  
Vol 11 (9) ◽  
pp. 3789
Author(s):  
Emmanouil D. Tsochatzis ◽  
Georgios Theodoridis ◽  
Helen G. Gika

Nuclear clarifying agents (NCAs) are a class of substances frequently used as additives in the production of polymers to improve their physical properties. Some are EU regulated under Commission Regulation (EU) no. 10/2011 can be used as additives in the production of food contact plastics. However, limited analytical methods for their analysis are currently available, in part due to poor solubility in most common organic solvents and lack of analytical standards of known purity. In this work, a simple and sensitive method was developed to analyze 4 EU-regulated sorbitol-based nucleating agents in food simulants, following solubility studies to establish effective solvents. The method was shown to be accurate and precise and can be used with official food simulant D1 (50% v/v ethanol/H2O). Application to other ethanolic simulants is also possible, but due to solubility issues, a posteriori conversion of those simulants into simulant D1 is required. Finally, the method was applied to quantify the target analytes in simulants after migration testing with polypropylene (PP) beverage cups.


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